Click here to switch to the map view.

The map label for this gene is ldhA [H]

Identifier: 15606122

GI number: 15606122

Start: 504614

End: 505618

Strand: Direct

Name: ldhA [H]

Synonym: aq_727

Alternate gene names: 15606122

Gene position: 504614-505618 (Clockwise)

Preceding gene: 15606121

Following gene: 15606123

Centisome position: 32.53

GC content: 40.4

Gene sequence:

>1005_bases
ATGAACGTATTATTTACAAGTGTTCCACAGGAAGATGTACCCTTCTACCAAGAGGCTTTAAAGGACCTATCACTAAAAAT
TTACACAACAGACGTTTCAAAAGTACCCGAAAATGAATTAAAAAAAGCGGAACTAATTTCAGTCTTTGTTTACGATAAGC
TTACGGAAGAATTACTTTCCAAGATGCCTAGGTTAAAGCTTATCCACACACGTTCCGTAGGTTTCGACCATATAGACTTA
GATTACTGCAAGAAGAAAGGCATTCTGGTTACTCACATACCTGCTTATTCTCCCGAATCTGTAGCTGAACACACCTTTGC
CATGATCCTTACTCTGGTAAAGAGGCTAAAGAGGATAGAGGATAGAGTAAAAAAACTGAACTTTTCGCAGGATAGCGAAA
TTCTGGCACGGGAGTTAAATAGGCTCACGCTTGGAGTTATCGGAACCGGAAGGATAGGAAGTAGAGTTGCTATGTACGGT
TTAGCGTTTGGAATGAAGGTTCTGTGTTACGATGTCGTTAAGAGGGAGGACTTAAAAGAAAAAGGATGTGTTTACACCTC
CCTGGATGAATTACTGAAGGAAAGCGATGTTATTTCCCTTCACGTTCCGTATACGAAAGAAACCCATCACATGATAAACG
AAGAAAGGATTAGTTTAATGAAGGACGGTGTTTATTTGATAAACACCGCGAGGGGAAAAGTTGTAGATACTGATGCACTT
TACAGAGCCTACCAGAGGGGTAAGTTTTCAGGCTTGGGACTGGACGTATTTGAGGACGAGGAAATCTTAATCCTGAAGAA
GTACACGGAAGGTAAGGCTACTGATAAAAACCTGAAAATACTCGAACTTGCCTGTAAAGATAACGTGATAATTACTCCCC
ATATAGCCTATTACACGGACAAATCCTTAGAGAGGATCAGGGAAGAAACAGTAAAAGTTGTTAAAGCGTTCGTGAAAGGA
GATCTGGAACAAATAAAGGGGAACTTTGTTGTAGGTCCTTCCTGA

Upstream 100 bases:

>100_bases
TAGAACACTGGTTTTACGTAATCTTAACAACATTATCAGTTTTCATCTTCATAGAAATAAGGAAATTAGTTTTTACAATT
TTCAGTGAGAGGTAATCATT

Downstream 100 bases:

>100_bases
AGCAAATATCTATAAAAGTATTTATATTTATCGGAATGACTAAGGTTGTAAATTACGGGGAAGTTCAGTACGGTTTTGCT
AAGAGGTACTTTGAAGAATT

Product: D-lactate dehydrogenase

Products: NA

Alternate protein names: D-LDH; D-specific D-2-hydroxyacid dehydrogenase [H]

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MNVLFTSVPQEDVPFYQEALKDLSLKIYTTDVSKVPENELKKAELISVFVYDKLTEELLSKMPRLKLIHTRSVGFDHIDL
DYCKKKGILVTHIPAYSPESVAEHTFAMILTLVKRLKRIEDRVKKLNFSQDSEILARELNRLTLGVIGTGRIGSRVAMYG
LAFGMKVLCYDVVKREDLKEKGCVYTSLDELLKESDVISLHVPYTKETHHMINEERISLMKDGVYLINTARGKVVDTDAL
YRAYQRGKFSGLGLDVFEDEEILILKKYTEGKATDKNLKILELACKDNVIITPHIAYYTDKSLERIREETVKVVKAFVKG
DLEQIKGNFVVGPS

Sequences:

>Translated_334_residues
MNVLFTSVPQEDVPFYQEALKDLSLKIYTTDVSKVPENELKKAELISVFVYDKLTEELLSKMPRLKLIHTRSVGFDHIDL
DYCKKKGILVTHIPAYSPESVAEHTFAMILTLVKRLKRIEDRVKKLNFSQDSEILARELNRLTLGVIGTGRIGSRVAMYG
LAFGMKVLCYDVVKREDLKEKGCVYTSLDELLKESDVISLHVPYTKETHHMINEERISLMKDGVYLINTARGKVVDTDAL
YRAYQRGKFSGLGLDVFEDEEILILKKYTEGKATDKNLKILELACKDNVIITPHIAYYTDKSLERIREETVKVVKAFVKG
DLEQIKGNFVVGPS
>Mature_334_residues
MNVLFTSVPQEDVPFYQEALKDLSLKIYTTDVSKVPENELKKAELISVFVYDKLTEELLSKMPRLKLIHTRSVGFDHIDL
DYCKKKGILVTHIPAYSPESVAEHTFAMILTLVKRLKRIEDRVKKLNFSQDSEILARELNRLTLGVIGTGRIGSRVAMYG
LAFGMKVLCYDVVKREDLKEKGCVYTSLDELLKESDVISLHVPYTKETHHMINEERISLMKDGVYLINTARGKVVDTDAL
YRAYQRGKFSGLGLDVFEDEEILILKKYTEGKATDKNLKILELACKDNVIITPHIAYYTDKSLERIREETVKVVKAFVKG
DLEQIKGNFVVGPS

Specific function: Fermentative Lactate Dehydrogenase. [C]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI61743967, Length=311, Percent_Identity=30.8681672025724, Blast_Score=145, Evalue=4e-35,
Organism=Homo sapiens, GI4557497, Length=311, Percent_Identity=30.8681672025724, Blast_Score=145, Evalue=4e-35,
Organism=Homo sapiens, GI23308577, Length=283, Percent_Identity=32.1554770318021, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI145580578, Length=322, Percent_Identity=31.055900621118, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI4557499, Length=322, Percent_Identity=31.055900621118, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI145580575, Length=322, Percent_Identity=31.055900621118, Blast_Score=134, Evalue=9e-32,
Organism=Homo sapiens, GI6912396, Length=290, Percent_Identity=29.3103448275862, Blast_Score=110, Evalue=1e-24,
Organism=Escherichia coli, GI1787645, Length=283, Percent_Identity=36.7491166077738, Blast_Score=179, Evalue=3e-46,
Organism=Escherichia coli, GI87082289, Length=256, Percent_Identity=33.203125, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI1789279, Length=305, Percent_Identity=30.8196721311475, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1788660, Length=313, Percent_Identity=28.1150159744409, Blast_Score=92, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17532191, Length=272, Percent_Identity=31.9852941176471, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI25147481, Length=292, Percent_Identity=30.1369863013699, Blast_Score=121, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6320925, Length=294, Percent_Identity=35.7142857142857, Blast_Score=159, Evalue=8e-40,
Organism=Saccharomyces cerevisiae, GI6322116, Length=294, Percent_Identity=35.7142857142857, Blast_Score=157, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6324055, Length=241, Percent_Identity=33.195020746888, Blast_Score=122, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6324964, Length=260, Percent_Identity=30.3846153846154, Blast_Score=111, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6324980, Length=135, Percent_Identity=31.1111111111111, Blast_Score=70, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6325144, Length=122, Percent_Identity=32.7868852459016, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI62472511, Length=288, Percent_Identity=34.375, Blast_Score=145, Evalue=6e-35,
Organism=Drosophila melanogaster, GI24646446, Length=288, Percent_Identity=34.375, Blast_Score=144, Evalue=6e-35,
Organism=Drosophila melanogaster, GI24646448, Length=288, Percent_Identity=34.375, Blast_Score=144, Evalue=6e-35,
Organism=Drosophila melanogaster, GI24646452, Length=288, Percent_Identity=34.375, Blast_Score=144, Evalue=6e-35,
Organism=Drosophila melanogaster, GI24646450, Length=288, Percent_Identity=34.375, Blast_Score=144, Evalue=6e-35,
Organism=Drosophila melanogaster, GI28574286, Length=312, Percent_Identity=27.5641025641026, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI28571528, Length=302, Percent_Identity=32.4503311258278, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI19921140, Length=269, Percent_Identity=27.5092936802974, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24585514, Length=307, Percent_Identity=26.3843648208469, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI28574282, Length=307, Percent_Identity=26.3843648208469, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI45552429, Length=307, Percent_Identity=26.3843648208469, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI28574284, Length=307, Percent_Identity=26.3843648208469, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI45551003, Length=307, Percent_Identity=26.3843648208469, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24585516, Length=263, Percent_Identity=25.0950570342205, Blast_Score=88, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.28 [H]

Molecular weight: Translated: 38072; Mature: 38072

Theoretical pI: Translated: 7.97; Mature: 7.97

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1 ; PS00670 D_2_HYDROXYACID_DH_2 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVLFTSVPQEDVPFYQEALKDLSLKIYTTDVSKVPENELKKAELISVFVYDKLTEELLS
CCEEECCCCCCCCCHHHHHHHHCEEEEEECCHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
KMPRLKLIHTRSVGFDHIDLDYCKKKGILVTHIPAYSPESVAEHTFAMILTLVKRLKRIE
HCCCEEEEEECCCCCCCCCHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
DRVKKLNFSQDSEILARELNRLTLGVIGTGRIGSRVAMYGLAFGMKVLCYDVVKREDLKE
HHHHHCCCCCCHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KGCVYTSLDELLKESDVISLHVPYTKETHHMINEERISLMKDGVYLINTARGKVVDTDAL
CCCEEHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCEEEEECCCCCEECHHHH
YRAYQRGKFSGLGLDVFEDEEILILKKYTEGKATDKNLKILELACKDNVIITPHIAYYTD
HHHHHCCCCCCCCCEEECCCCEEEEEECCCCCCCCCCCEEEEEECCCCEEECCCHHHHCC
KSLERIREETVKVVKAFVKGDLEQIKGNFVVGPS
HHHHHHHHHHHHHHHHHHHCCHHHHCCCEEECCC
>Mature Secondary Structure
MNVLFTSVPQEDVPFYQEALKDLSLKIYTTDVSKVPENELKKAELISVFVYDKLTEELLS
CCEEECCCCCCCCCHHHHHHHHCEEEEEECCHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
KMPRLKLIHTRSVGFDHIDLDYCKKKGILVTHIPAYSPESVAEHTFAMILTLVKRLKRIE
HCCCEEEEEECCCCCCCCCHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
DRVKKLNFSQDSEILARELNRLTLGVIGTGRIGSRVAMYGLAFGMKVLCYDVVKREDLKE
HHHHHCCCCCCHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KGCVYTSLDELLKESDVISLHVPYTKETHHMINEERISLMKDGVYLINTARGKVVDTDAL
CCCEEHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCEEEEECCCCCEECHHHH
YRAYQRGKFSGLGLDVFEDEEILILKKYTEGKATDKNLKILELACKDNVIITPHIAYYTD
HHHHHCCCCCCCCCEEECCCCEEEEEECCCCCCCCCCCEEEEEECCCCEEECCCHHHHCC
KSLERIREETVKVVKAFVKGDLEQIKGNFVVGPS
HHHHHHHHHHHHHHHHHHHCCHHHHCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9665876 [H]