Click here to switch to the map view.

The map label for this gene is glgB

Identifier: 15606119

GI number: 15606119

Start: 498743

End: 500635

Strand: Direct

Name: glgB

Synonym: aq_722

Alternate gene names: 15606119

Gene position: 498743-500635 (Clockwise)

Preceding gene: 15606118

Following gene: 15606120

Centisome position: 32.15

GC content: 46.38

Gene sequence:

>1893_bases
ATGAAGAAGTTCAGTCTCATCAGTGATTACGACGTTTACCTCTTTAAGGAGGGAACGCACACGAGACTTTACGATAAACT
TGGCTCCCACGTTATAGAACTAAACGGGAAAAGGTATACCTTCTTTGCGGTTTGGGCACCCCACGCGGATTACGTATCAC
TTATAGGCGATTTTAACGAATGGGATAAAGGTTCTACTCCCATGGTAAAGAGGGAGGACGGCTCCGGAATATGGGAGGTT
TTACTTGAAGGAGACCTGACTGGTTCAAAGTACAAGTACTTTATAAAGAACGGGAATTACGAAGTTGATAAGTCCGATCC
CTTCGCATTTTTCTGTGAGCAACCCCCCGGAAACGCTTCCGTAGTGTGGAAGCTCAATTACAGGTGGAACGACTCCGAAT
ACATGAAAAAGAGGAAAAGAGTAAACTCACACGACTCGCCTATATCCATATACGAAGTTCACGTGGGTTCTTGGAGGAGA
GTTCCAGAAGAGGGAAACAGATTTTTGAGCTATAGGGAACTTGCCGAATACCTCCCATACTACGTAAAAGAGATGGGATT
TACTCACGTTGAGTTCTTACCCGTTATGGAACATCCCTTTTACGGCTCTTGGGGCTACCAGATAACGGGCTACTTCGCTC
CGACTTCCAGATACGGAACTCCTCAGGACTTTATGTACTTAATAGACAAACTTCATCAAGAAGGGATAGGTGTGATACTA
GACTGGGTTCCCTCTCACTTTCCCACCGATGCCCACGGGCTCGCATACTTTGACGGGACTCACCTTTACGAGTACGAGGA
CTGGAGAAAGAGGTGGCATCCCGACTGGAACAGCTTTGTTTTTGATTACGGAAAACCGGAAGTTCGCTCCTTTCTCCTGA
GTTCTGCCCACTTCTGGCTCGACAAGTACCACGCAGACGGTCTCAGAGTGGATGCAGTTGCTTCAATGCTTTACCTAGAT
TACTCTAGGAAAGAATGGGTTCCAAACATATACGGAGGGAAAGAAAACCTCGAGGCTATAGAATTCCTCAGGAAGTTTAA
CGAAAGCGTTTACAGAAATTTTCCAGACGTCCAGACAATAGCGGAGGAATCAACAGCCTGGCCTATGGTGTCCAGACCTA
CATACGTGGGGGGACTGGGATTTGGAATGAAGTGGAATATGGGTTGGATGAACGACACACTCTTTTACTTTTCAAAGGAT
CCCATCTACAGGAAGTACCACCATGAAGTCCTCACTTTCAGTATATGGTACGCTTTTTCCGAGAACTTCGTCCTTCCACT
ATCCCACGATGAAGTTGTTCACGGAAAGGGTTCTCTGATAGGGAAGATGCCAGGAGATTACTGGCAGAAGTTTGCAAACC
TTAGAGCCCTTTTCGGATACATGTGGGCACACCCAGGGAAAAAACTCCTCTTTATGGGGGGAGAGTTCGGACAGTTTAAG
GAATGGGATCACGAAACGAGTCTCGACTGGCACCTCTTGGAATACCCTTCTCACAGAGGTATTCAGAGATTAGTTAAGGA
CTTAAACGAAGTTTACAGGAGGGAAAAGGCTTTGCACGAAACGGATTTTTCACCTGAGGGCTTTGAGTGGGTAGACTTCC
ACGACTGGGAAAAGAGCGTTATATCCTTCTTGAGAAAGGACAAAAGCGGTAAGGAAATTATACTCGTAGTTTGCAACTTC
ACACCCGTTCCGAGATACGATTACAGGGTAGGTGTACCGAAAGGCGGATACTGGAGGGAGATAATGAATACCGATGCAAA
GGAGTACTGGGGCTCCGGAATGGGAAATCTGGGTGGAAAAGAGGCTGATAAAATCCCGTGGCACGGAAGAAAATTCTCAC
TTTCACTTACCCTGCCTCCCCTTTCCGTGATCTATTTAAAGCACGAAGGATGA

Upstream 100 bases:

>100_bases
GAACTTTACGAAAATGCGAAGAAGTTCAGAAATTCCGTTAAGATAGTGATGAGCCTTGACTTTTCCTGCGATAGGATGAC
AAAAGAATACATAGAGTGCT

Downstream 100 bases:

>100_bases
GATTGGCAGGTATTTTACTTCACGTAACTTCACTTCCCTCTCCTTACGGGATAGGGGATCTCGGAAAAGAAGCCTACAGG
TTTCTGGACTTCTTAAAGGA

Product: glycogen branching enzyme

Products: NA

Alternate protein names: 1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase; Glycogen-branching enzyme; BE

Number of amino acids: Translated: 630; Mature: 630

Protein sequence:

>630_residues
MKKFSLISDYDVYLFKEGTHTRLYDKLGSHVIELNGKRYTFFAVWAPHADYVSLIGDFNEWDKGSTPMVKREDGSGIWEV
LLEGDLTGSKYKYFIKNGNYEVDKSDPFAFFCEQPPGNASVVWKLNYRWNDSEYMKKRKRVNSHDSPISIYEVHVGSWRR
VPEEGNRFLSYRELAEYLPYYVKEMGFTHVEFLPVMEHPFYGSWGYQITGYFAPTSRYGTPQDFMYLIDKLHQEGIGVIL
DWVPSHFPTDAHGLAYFDGTHLYEYEDWRKRWHPDWNSFVFDYGKPEVRSFLLSSAHFWLDKYHADGLRVDAVASMLYLD
YSRKEWVPNIYGGKENLEAIEFLRKFNESVYRNFPDVQTIAEESTAWPMVSRPTYVGGLGFGMKWNMGWMNDTLFYFSKD
PIYRKYHHEVLTFSIWYAFSENFVLPLSHDEVVHGKGSLIGKMPGDYWQKFANLRALFGYMWAHPGKKLLFMGGEFGQFK
EWDHETSLDWHLLEYPSHRGIQRLVKDLNEVYRREKALHETDFSPEGFEWVDFHDWEKSVISFLRKDKSGKEIILVVCNF
TPVPRYDYRVGVPKGGYWREIMNTDAKEYWGSGMGNLGGKEADKIPWHGRKFSLSLTLPPLSVIYLKHEG

Sequences:

>Translated_630_residues
MKKFSLISDYDVYLFKEGTHTRLYDKLGSHVIELNGKRYTFFAVWAPHADYVSLIGDFNEWDKGSTPMVKREDGSGIWEV
LLEGDLTGSKYKYFIKNGNYEVDKSDPFAFFCEQPPGNASVVWKLNYRWNDSEYMKKRKRVNSHDSPISIYEVHVGSWRR
VPEEGNRFLSYRELAEYLPYYVKEMGFTHVEFLPVMEHPFYGSWGYQITGYFAPTSRYGTPQDFMYLIDKLHQEGIGVIL
DWVPSHFPTDAHGLAYFDGTHLYEYEDWRKRWHPDWNSFVFDYGKPEVRSFLLSSAHFWLDKYHADGLRVDAVASMLYLD
YSRKEWVPNIYGGKENLEAIEFLRKFNESVYRNFPDVQTIAEESTAWPMVSRPTYVGGLGFGMKWNMGWMNDTLFYFSKD
PIYRKYHHEVLTFSIWYAFSENFVLPLSHDEVVHGKGSLIGKMPGDYWQKFANLRALFGYMWAHPGKKLLFMGGEFGQFK
EWDHETSLDWHLLEYPSHRGIQRLVKDLNEVYRREKALHETDFSPEGFEWVDFHDWEKSVISFLRKDKSGKEIILVVCNF
TPVPRYDYRVGVPKGGYWREIMNTDAKEYWGSGMGNLGGKEADKIPWHGRKFSLSLTLPPLSVIYLKHEG
>Mature_630_residues
MKKFSLISDYDVYLFKEGTHTRLYDKLGSHVIELNGKRYTFFAVWAPHADYVSLIGDFNEWDKGSTPMVKREDGSGIWEV
LLEGDLTGSKYKYFIKNGNYEVDKSDPFAFFCEQPPGNASVVWKLNYRWNDSEYMKKRKRVNSHDSPISIYEVHVGSWRR
VPEEGNRFLSYRELAEYLPYYVKEMGFTHVEFLPVMEHPFYGSWGYQITGYFAPTSRYGTPQDFMYLIDKLHQEGIGVIL
DWVPSHFPTDAHGLAYFDGTHLYEYEDWRKRWHPDWNSFVFDYGKPEVRSFLLSSAHFWLDKYHADGLRVDAVASMLYLD
YSRKEWVPNIYGGKENLEAIEFLRKFNESVYRNFPDVQTIAEESTAWPMVSRPTYVGGLGFGMKWNMGWMNDTLFYFSKD
PIYRKYHHEVLTFSIWYAFSENFVLPLSHDEVVHGKGSLIGKMPGDYWQKFANLRALFGYMWAHPGKKLLFMGGEFGQFK
EWDHETSLDWHLLEYPSHRGIQRLVKDLNEVYRREKALHETDFSPEGFEWVDFHDWEKSVISFLRKDKSGKEIILVVCNF
TPVPRYDYRVGVPKGGYWREIMNTDAKEYWGSGMGNLGGKEADKIPWHGRKFSLSLTLPPLSVIYLKHEG

Specific function: Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position

COG id: COG0296

COG function: function code G; 1,4-alpha-glucan branching enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family

Homologues:

Organism=Homo sapiens, GI189458812, Length=667, Percent_Identity=27.1364317841079, Blast_Score=182, Evalue=1e-45,
Organism=Escherichia coli, GI1789839, Length=631, Percent_Identity=56.418383518225, Blast_Score=694, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17554896, Length=635, Percent_Identity=26.7716535433071, Blast_Score=194, Evalue=9e-50,
Organism=Caenorhabditis elegans, GI32564391, Length=626, Percent_Identity=25.7188498402556, Blast_Score=173, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6320826, Length=642, Percent_Identity=28.0373831775701, Blast_Score=188, Evalue=2e-48,
Organism=Drosophila melanogaster, GI28573410, Length=600, Percent_Identity=29.5, Blast_Score=186, Evalue=4e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLGB_AQUAE (O66936)

Other databases:

- EMBL:   AE000657
- PIR:   D70363
- RefSeq:   NP_213496.1
- ProteinModelPortal:   O66936
- SMR:   O66936
- GeneID:   1193385
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_722
- NMPDR:   fig|224324.1.peg.511
- HOGENOM:   HBG287139
- OMA:   RVYHQNG
- PhylomeDB:   O66936
- ProtClustDB:   PRK12313
- BioCyc:   AAEO224324:AQ_722-MONOMER
- BRENDA:   2.4.1.18
- HAMAP:   MF_00685
- InterPro:   IPR006407
- InterPro:   IPR006048
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- Gene3D:   G3DSA:2.60.40.1180
- Gene3D:   G3DSA:3.20.20.80
- Gene3D:   G3DSA:2.60.40.10
- TIGRFAMs:   TIGR01515

Pfam domain/function: PF00128 Alpha-amylase; PF02806 Alpha-amylase_C; PF02922 CBM_48; SSF51445 Glyco_hydro_cat

EC number: =2.4.1.18

Molecular weight: Translated: 74168; Mature: 74168

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: NA

Important sites: ACT_SITE 206-206 ACT_SITE 241-241 ACT_SITE 246-246 ACT_SITE 309-309 ACT_SITE 311-311 ACT_SITE 362-362 ACT_SITE 429-429 ACT_SITE 430-430

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKFSLISDYDVYLFKEGTHTRLYDKLGSHVIELNGKRYTFFAVWAPHADYVSLIGDFNE
CCCCCEECCCEEEEEECCCCHHHHHHHCCEEEEECCCEEEEEEEECCCCHHHHHHCCCCC
WDKGSTPMVKREDGSGIWEVLLEGDLTGSKYKYFIKNGNYEVDKSDPFAFFCEQPPGNAS
CCCCCCCCEEECCCCCEEEEEEECCCCCCEEEEEEECCCEEECCCCCEEEEECCCCCCCE
VVWKLNYRWNDSEYMKKRKRVNSHDSPISIYEVHVGSWRRVPEEGNRFLSYRELAEYLPY
EEEEEEEEECCHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
YVKEMGFTHVEFLPVMEHPFYGSWGYQITGYFAPTSRYGTPQDFMYLIDKLHQEGIGVIL
HHHHCCCCEEEEEEHHCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEE
DWVPSHFPTDAHGLAYFDGTHLYEYEDWRKRWHPDWNSFVFDYGKPEVRSFLLSSAHFWL
ECCCCCCCCCCCCEEEECCCEEEECHHHHHHCCCCHHHEEEECCCHHHHHHHHHHHHHHH
DKYHADGLRVDAVASMLYLDYSRKEWVPNIYGGKENLEAIEFLRKFNESVYRNFPDVQTI
HHHCCCCEEHHHHHHHHHHCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHH
AEESTAWPMVSRPTYVGGLGFGMKWNMGWMNDTLFYFSKDPIYRKYHHEVLTFSIWYAFS
HHCCCCCCCCCCCCEEECCCCCCEECCCCCCCEEEEEECCCHHHHHHHHEEEEEEEEEEC
ENFVLPLSHDEVVHGKGSLIGKMPGDYWQKFANLRALFGYMWAHPGKKLLFMGGEFGQFK
CCEEEECCCCCEECCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCC
EWDHETSLDWHLLEYPSHRGIQRLVKDLNEVYRREKALHETDFSPEGFEWVDFHDWEKSV
CCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCHHHHHH
ISFLRKDKSGKEIILVVCNFTPVPRYDYRVGVPKGGYWREIMNTDAKEYWGSGMGNLGGK
HHHHHCCCCCCEEEEEEECCCCCCCCCEEECCCCCCHHHHHHCCCHHHHHCCCCCCCCCC
EADKIPWHGRKFSLSLTLPPLSVIYLKHEG
CCCCCCCCCCEEEEEEECCCEEEEEEEECC
>Mature Secondary Structure
MKKFSLISDYDVYLFKEGTHTRLYDKLGSHVIELNGKRYTFFAVWAPHADYVSLIGDFNE
CCCCCEECCCEEEEEECCCCHHHHHHHCCEEEEECCCEEEEEEEECCCCHHHHHHCCCCC
WDKGSTPMVKREDGSGIWEVLLEGDLTGSKYKYFIKNGNYEVDKSDPFAFFCEQPPGNAS
CCCCCCCCEEECCCCCEEEEEEECCCCCCEEEEEEECCCEEECCCCCEEEEECCCCCCCE
VVWKLNYRWNDSEYMKKRKRVNSHDSPISIYEVHVGSWRRVPEEGNRFLSYRELAEYLPY
EEEEEEEEECCHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
YVKEMGFTHVEFLPVMEHPFYGSWGYQITGYFAPTSRYGTPQDFMYLIDKLHQEGIGVIL
HHHHCCCCEEEEEEHHCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEE
DWVPSHFPTDAHGLAYFDGTHLYEYEDWRKRWHPDWNSFVFDYGKPEVRSFLLSSAHFWL
ECCCCCCCCCCCCEEEECCCEEEECHHHHHHCCCCHHHEEEECCCHHHHHHHHHHHHHHH
DKYHADGLRVDAVASMLYLDYSRKEWVPNIYGGKENLEAIEFLRKFNESVYRNFPDVQTI
HHHCCCCEEHHHHHHHHHHCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHH
AEESTAWPMVSRPTYVGGLGFGMKWNMGWMNDTLFYFSKDPIYRKYHHEVLTFSIWYAFS
HHCCCCCCCCCCCCEEECCCCCCEECCCCCCCEEEEEECCCHHHHHHHHEEEEEEEEEEC
ENFVLPLSHDEVVHGKGSLIGKMPGDYWQKFANLRALFGYMWAHPGKKLLFMGGEFGQFK
CCEEEECCCCCEECCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCC
EWDHETSLDWHLLEYPSHRGIQRLVKDLNEVYRREKALHETDFSPEGFEWVDFHDWEKSV
CCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCHHHHHH
ISFLRKDKSGKEIILVVCNFTPVPRYDYRVGVPKGGYWREIMNTDAKEYWGSGMGNLGGK
HHHHHCCCCCCEEEEEEECCCCCCCCCEEECCCCCCHHHHHHCCCHHHHHCCCCCCCCCC
EADKIPWHGRKFSLSLTLPPLSVIYLKHEG
CCCCCCCCCCEEEEEEECCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320