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The map label for this gene is hypB [H]

Identifier: 15606084

GI number: 15606084

Start: 456335

End: 457114

Strand: Direct

Name: hypB [H]

Synonym: aq_671

Alternate gene names: 15606084

Gene position: 456335-457114 (Clockwise)

Preceding gene: 15606083

Following gene: 15606085

Centisome position: 29.42

GC content: 46.15

Gene sequence:

>780_bases
ATGTGTAAAGACTGCGGATGTTCCATCCCTGAGCACAACCACGAGCATGAACACCATCATCACCATCACCATACAAATCC
CGCACTCGGTGATAAGAAGACCGTTGAGGTCCTGAAAAAGATACTCTCCGCAAACGACGAGCAGGCGGAGAGCAACAGGA
GGCACTTTGAAAGGCACGGAATACTGGCTGTTAACTTGATGAGTTCCCCGGGATCGGGAAAGACGACACTCCTTGAAAGA
ACCATAGAACTCCTGAAAGACGAACTCAAGATAGGTGTAATTGAAGGAGATTTGGAAACAAACAGGGACGCGGAGAGGAT
TAAAAAGAAGGGGGCGCCGGCATATCAGATAACAACGGGACAGGCTTGCCACCTTGATGCCTTTATGGTTCACGAAGGCA
TACACCACCTTCCCTTAGAAGAACTTGATATTGTCTTCATAGAAAACGTCGGAAACTTAGTTTGTCCGGCTTCTTACGAC
GTGGGAGCACACCTCAACGTCGTTCTCCTCTCCACAACAGAAGGAGAAGACAAGCCAGAGAAGTATCCGGTGATGTTTAA
AAACTCTCAATTGATGTTAATAACTAAGGCAGATCTCTTACCTTACATGGACTTTGACGTTGAAAAGGCTGTAGAGTCCG
CAAGAAAGGTAAATCCTTCCCTTGACGTCATTACGCTTTCCGCAAAAACTGGAGAGGGAATGGAATTGTGGCTTGATTAT
CTGAAGTTTAAGTTGAAGGTCCTCAGGAATGCCCTTCAAAAGGCTAAAGCTTCACCTTAA

Upstream 100 bases:

>100_bases
AAACTCGCATCAGCCCAAAAGGAAGACGTACAGGAGAGTATTAAGTACCTTGAAGAAAAGATTTCGGAGATTGAAACTAA
ATTAAAAGAGGAGGAGAAAA

Downstream 100 bases:

>100_bases
AGGGGCCGTTCAGGGTGTCGGGTTTCGCCCCTTCGTTTACAGAATAGCCAAAGAGCTGGGACTAAAAGGTTTCGTTATAA
ACGACTCAAAAGGTGTTTAC

Product: hydrogenase expression/formation protein B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MCKDCGCSIPEHNHEHEHHHHHHHTNPALGDKKTVEVLKKILSANDEQAESNRRHFERHGILAVNLMSSPGSGKTTLLER
TIELLKDELKIGVIEGDLETNRDAERIKKKGAPAYQITTGQACHLDAFMVHEGIHHLPLEELDIVFIENVGNLVCPASYD
VGAHLNVVLLSTTEGEDKPEKYPVMFKNSQLMLITKADLLPYMDFDVEKAVESARKVNPSLDVITLSAKTGEGMELWLDY
LKFKLKVLRNALQKAKASP

Sequences:

>Translated_259_residues
MCKDCGCSIPEHNHEHEHHHHHHHTNPALGDKKTVEVLKKILSANDEQAESNRRHFERHGILAVNLMSSPGSGKTTLLER
TIELLKDELKIGVIEGDLETNRDAERIKKKGAPAYQITTGQACHLDAFMVHEGIHHLPLEELDIVFIENVGNLVCPASYD
VGAHLNVVLLSTTEGEDKPEKYPVMFKNSQLMLITKADLLPYMDFDVEKAVESARKVNPSLDVITLSAKTGEGMELWLDY
LKFKLKVLRNALQKAKASP
>Mature_259_residues
MCKDCGCSIPEHNHEHEHHHHHHHTNPALGDKKTVEVLKKILSANDEQAESNRRHFERHGILAVNLMSSPGSGKTTLLER
TIELLKDELKIGVIEGDLETNRDAERIKKKGAPAYQITTGQACHLDAFMVHEGIHHLPLEELDIVFIENVGNLVCPASYD
VGAHLNVVLLSTTEGEDKPEKYPVMFKNSQLMLITKADLLPYMDFDVEKAVESARKVNPSLDVITLSAKTGEGMELWLDY
LKFKLKVLRNALQKAKASP

Specific function: Could be involved in nickel binding and accumulation. Binds 3.9 nickel ions per molecule [H]

COG id: COG0378

COG function: function code OK; Ni2+-binding GTPase involved in regulation of expression and maturation of urease and hydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hypB/hupM family [H]

Homologues:

Organism=Escherichia coli, GI1789082, Length=236, Percent_Identity=49.5762711864407, Blast_Score=242, Evalue=2e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003495
- InterPro:   IPR004392
- InterPro:   IPR012202 [H]

Pfam domain/function: PF02492 cobW [H]

EC number: NA

Molecular weight: Translated: 29016; Mature: 29016

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: PS00028 ZINC_FINGER_C2H2_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCKDCGCSIPEHNHEHEHHHHHHHTNPALGDKKTVEVLKKILSANDEQAESNRRHFERHG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCC
ILAVNLMSSPGSGKTTLLERTIELLKDELKIGVIEGDLETNRDAERIKKKGAPAYQITTG
EEEEEEECCCCCCHHHHHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHCCCCEEEEECC
QACHLDAFMVHEGIHHLPLEELDIVFIENVGNLVCPASYDVGAHLNVVLLSTTEGEDKPE
CCCCHHHHHHHCCCCCCCHHHHCEEEEECCCCEECCCCCCCCCCEEEEEEEECCCCCCCC
KYPVMFKNSQLMLITKADLLPYMDFDVEKAVESARKVNPSLDVITLSAKTGEGMELWLDY
CCCEEEECCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHH
LKFKLKVLRNALQKAKASP
HHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MCKDCGCSIPEHNHEHEHHHHHHHTNPALGDKKTVEVLKKILSANDEQAESNRRHFERHG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCC
ILAVNLMSSPGSGKTTLLERTIELLKDELKIGVIEGDLETNRDAERIKKKGAPAYQITTG
EEEEEEECCCCCCHHHHHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHCCCCEEEEECC
QACHLDAFMVHEGIHHLPLEELDIVFIENVGNLVCPASYDVGAHLNVVLLSTTEGEDKPE
CCCCHHHHHHHCCCCCCCHHHHCEEEEECCCCEECCCCCCCCCCEEEEEEEECCCCCCCC
KYPVMFKNSQLMLITKADLLPYMDFDVEKAVESARKVNPSLDVITLSAKTGEGMELWLDY
CCCEEEECCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHH
LKFKLKVLRNALQKAKASP
HHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8326860 [H]