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The map label for this gene is hupD [H]

Identifier: 15606081

GI number: 15606081

Start: 455140

End: 455628

Strand: Direct

Name: hupD [H]

Synonym: aq_667

Alternate gene names: 15606081

Gene position: 455140-455628 (Clockwise)

Preceding gene: 15606080

Following gene: 15606082

Centisome position: 29.34

GC content: 43.97

Gene sequence:

>489_bases
ATGATTACGGTACTCGGTATAGGGAACATACTCTTATCGGATGAAGGACTGGGAGTGAGAACGGTTGAGGAGCTCCAGAG
GAGGTACGAGTTTCCCGAAAACGTGAAAGTGTTGGACGGCGGAACTCTCGGGATAGACTTACTTTACCATATAGAGGGAA
CGGAAAAACTCCTCATAATTGATGCGGTTCTAGGGGGAAAGAAACCTGGAACCTTTTACAAAATAAAAAACGAAAATGTA
AAGAAGTACTTTAAAAGTAAAGTTTCCATGCACGAACTCGGAATTCAGGAAGTTCTTGCACTCTTAGAGGTTCTGGAAAA
ACCCATAAAAGAAATAGTAGTTCTGGGACTGGAGCCCAAAAGCCTTGAAGTTTCAACAGAGCTCACTCCGGAAATACAGG
AAAAAATTCCCCTCCTCGTGGAAGAAGCCCTATCTCAACTAAAGGAGTGGGGCGTTGAGGTGAAGGAGAAGAGCCATGAG
CAGTGTTAA

Upstream 100 bases:

>100_bases
CACCGGATTGCTTTTCGGGAAGGCTCTTAAAGAGAAACTCGTTAGGGTATCGGGACTACTCCTCCTTGCCCTTGCCTTCC
TCCTTTGATACCCTTTTCCC

Downstream 100 bases:

>100_bases
GTTTTTACAAAATTTAAAAAGGATAAACGAGGAAGATTTGAGGAACTTAATGGAAAAAGGTGAGACCTTTGTCCTTTACG
TGAGAAGCGAAAGGCTCTAC

Product: HupD hydrogenase related function

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 162; Mature: 162

Protein sequence:

>162_residues
MITVLGIGNILLSDEGLGVRTVEELQRRYEFPENVKVLDGGTLGIDLLYHIEGTEKLLIIDAVLGGKKPGTFYKIKNENV
KKYFKSKVSMHELGIQEVLALLEVLEKPIKEIVVLGLEPKSLEVSTELTPEIQEKIPLLVEEALSQLKEWGVEVKEKSHE
QC

Sequences:

>Translated_162_residues
MITVLGIGNILLSDEGLGVRTVEELQRRYEFPENVKVLDGGTLGIDLLYHIEGTEKLLIIDAVLGGKKPGTFYKIKNENV
KKYFKSKVSMHELGIQEVLALLEVLEKPIKEIVVLGLEPKSLEVSTELTPEIQEKIPLLVEEALSQLKEWGVEVKEKSHE
QC
>Mature_162_residues
MITVLGIGNILLSDEGLGVRTVEELQRRYEFPENVKVLDGGTLGIDLLYHIEGTEKLLIIDAVLGGKKPGTFYKIKNENV
KKYFKSKVSMHELGIQEVLALLEVLEKPIKEIVVLGLEPKSLEVSTELTPEIQEKIPLLVEEALSQLKEWGVEVKEKSHE
QC

Specific function: Not known. Could be involved in the processing of hydrogenase [H]

COG id: COG0680

COG function: function code C; Ni,Fe-hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A31 family [H]

Homologues:

Organism=Escherichia coli, GI1789367, Length=162, Percent_Identity=41.358024691358, Blast_Score=122, Evalue=1e-29,
Organism=Escherichia coli, GI1787209, Length=163, Percent_Identity=36.1963190184049, Blast_Score=107, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004419
- InterPro:   IPR000671 [H]

Pfam domain/function: PF01750 HycI [H]

EC number: 3.4.24.- [C]

Molecular weight: Translated: 18178; Mature: 18178

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITVLGIGNILLSDEGLGVRTVEELQRRYEFPENVKVLDGGTLGIDLLYHIEGTEKLLII
CEEEEEECEEEECCCCCCHHHHHHHHHHCCCCCCCEEEECCCEEEEEEEEECCCCEEEEE
DAVLGGKKPGTFYKIKNENVKKYFKSKVSMHELGIQEVLALLEVLEKPIKEIVVLGLEPK
EEECCCCCCCCEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHEEECCCCC
SLEVSTELTPEIQEKIPLLVEEALSQLKEWGVEVKEKSHEQC
CEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCC
>Mature Secondary Structure
MITVLGIGNILLSDEGLGVRTVEELQRRYEFPENVKVLDGGTLGIDLLYHIEGTEKLLII
CEEEEEECEEEECCCCCCHHHHHHHHHHCCCCCCCEEEECCCEEEEEEEEECCCCEEEEE
DAVLGGKKPGTFYKIKNENVKKYFKSKVSMHELGIQEVLALLEVLEKPIKEIVVLGLEPK
EEECCCCCCCCEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHEEECCCCC
SLEVSTELTPEIQEKIPLLVEEALSQLKEWGVEVKEKSHEQC
CEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8230232; 8163174; 12597275 [H]