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The map label for this gene is mbhL1 [H]

Identifier: 15606078

GI number: 15606078

Start: 451992

End: 453893

Strand: Direct

Name: mbhL1 [H]

Synonym: aq_662

Alternate gene names: 15606078

Gene position: 451992-453893 (Clockwise)

Preceding gene: 15606077

Following gene: 15606079

Centisome position: 29.14

GC content: 49.05

Gene sequence:

>1902_bases
ATGAAGAGAGTTGTAGTTGATCCTGTAACAAGGATAGAAGGTCATTTGAGAATTGAGATAATGGTTGACGAGGAAACAGG
ACAAGTGAAAGACGCCCTCTCCGCCGGAACTATGTGGAGGGGGATTGAACTCATAGTGAGAAACAGGGACCCGAGAGACG
TATGGGCATTTACCCAGAGAATATGCGGAGTTTGCACGTCCATACACGCCCTCGCATCACTGAGAGCTGTAGAAGACGCA
CTTGAGATAACTATTCCCAAAAACGCAAACTACATAAGGAATATAATGTACGGTTCACTTCAGGTTCACGACCACGTGGT
TCACTTTTACCACCTCCATGCACTCGACTGGGTTTCTCCTGTAGAAGCCCTGAAGGCGGACCCCGTCGCCACAGCGGCAC
TTGCAAACAAAATCCTTGAAAAGTACGGTGTTCTGAACGAATTTATGCCCGACTTTTTAGGCCACAGGGCTTACCCCAAA
AAGTTCCCCAAAGCCACTCCCGGTTACTTCAGGGAGTTTCAGAAGAAGATAAAGAAGCTCGTTGAGAGCGGACAGCTCGG
AATATTTGCCGCACACTGGTGGGATCACCCAGATTACCAGATGCTCCCGCCGGAAGTTCACCTCATAGGAATAGCCCACT
ACCTAAACATGCTTGACGTCCAGAGAGAACTCTTTATACCTCAGGTGGTGTTCGGCGGAAAGAACCCCCACCCCCACTAC
ATAGTTGGAGGAGTTAACTGTTCAATCTCCATGGACGATATGAACGCACCCGTAAACGCGGAAAGACTCGCGGTCGTAGA
AGACGCTATATACACTCAGGTAGAGTCCACAGACTTTTTCTACATTCCCGACATTCTCGCAATCGCCGATATATACCTCA
ATCAACATAACTGGTTCTACGGCGGTGGACTTTCCAAGAAGAGGGTAATAGGATACGGAGACTATCCCGATGAGCCCTAC
ACGGGCATAAAGAACGGGGATTACCACAAGAAGATACTGTGGCACTCAAACGGAGTCGTTGAAGACTTTTACAAGGGTGT
GGAAAAGGCCAAGTTCTACAACCTAGAAGGAAAGGACTTTACGGATCCAGAGCAAATTCAGGAGTTCGTCACCCACTCCT
GGTATAAGTATCCCGACGAAACGAAGGGACTTCACCCCTGGGATGGAATAACCGAGCCGAATTACACCGGACCGAAGGAA
GGAACGAAAACCCACTGGAAGTACCTTGACGAAAACGGAAAGTACTCTTGGATAAAAGCTCCGAGATGGAGAGGAAAGGC
CTGTGAAGTAGGACCTCTGGCAAGGTACATAATCGTCTACACAAAAGTAAAGCAGGGACACATAAAACCCACCTGGGTAG
ACGAACTCATAGTCAATCAAATAGACACCGTGTCCAAGATATTGAACCTCCCTCCCGAAAAGTGGCTGCCCACTACGGTC
GGAAGGACAATCGCGAGGGCACTGGAAGCCCAGATGTCAGCACATACAAACCTCTACTGGATGAAGAAACTCTATGACAA
CATAAAGGCGGGAGACACGAGCGTGGCAAACATGGAAAAGTGGGACCCCTCCACATGGCCAAAAGAGGCAAAGGGAGTGG
GATTGACGGAAGCCCCGAGAGGTGCTCTCGGACACTGGGTGATAATAAAAGACGGAAAAGTGGCGAATTATCAGTGCGTT
GTTCCAACCACATGGAACGGTTCTCCCAAGGACCCGAAGGGACAGCACGGAGCATTTGAAGAGTCAATGATTGACACGAA
GGTAAAGGTTCCCGAAAAGCCCCTTGAAGTTCTCAGAGGTATTCACTCATTTGACCCGTGCCTTGCATGTTCAACGCACC
TCTACAACGAAAAGGGAGAAGAGATAGCCAGCGTAAGGGTTCAGGGGGTGGTTCATGTTTAA

Upstream 100 bases:

>100_bases
CTATTGCGGCGGCAGCTGCTGCAGGCGGTGCAATAATTCACGGTATAGCTTCCAAGATAAGAAAGTCAGGTGAAAAGGAA
GAATAAGGGAGGTGTTATAG

Downstream 100 bases:

>100_bases
GAGGATTTTTATATTCAGCGCTTCCTTGAGGATATGGCACTGGATAAACTTCCTCACCATAATGGTCTTATTTTTTACGG
GACTCTACATAGGGACGCCT

Product: hydrogenase large subunit

Products: NA

Alternate protein names: Hydrogenlyase; Membrane-bound hydrogenase large subunit [H]

Number of amino acids: Translated: 633; Mature: 633

Protein sequence:

>633_residues
MKRVVVDPVTRIEGHLRIEIMVDEETGQVKDALSAGTMWRGIELIVRNRDPRDVWAFTQRICGVCTSIHALASLRAVEDA
LEITIPKNANYIRNIMYGSLQVHDHVVHFYHLHALDWVSPVEALKADPVATAALANKILEKYGVLNEFMPDFLGHRAYPK
KFPKATPGYFREFQKKIKKLVESGQLGIFAAHWWDHPDYQMLPPEVHLIGIAHYLNMLDVQRELFIPQVVFGGKNPHPHY
IVGGVNCSISMDDMNAPVNAERLAVVEDAIYTQVESTDFFYIPDILAIADIYLNQHNWFYGGGLSKKRVIGYGDYPDEPY
TGIKNGDYHKKILWHSNGVVEDFYKGVEKAKFYNLEGKDFTDPEQIQEFVTHSWYKYPDETKGLHPWDGITEPNYTGPKE
GTKTHWKYLDENGKYSWIKAPRWRGKACEVGPLARYIIVYTKVKQGHIKPTWVDELIVNQIDTVSKILNLPPEKWLPTTV
GRTIARALEAQMSAHTNLYWMKKLYDNIKAGDTSVANMEKWDPSTWPKEAKGVGLTEAPRGALGHWVIIKDGKVANYQCV
VPTTWNGSPKDPKGQHGAFEESMIDTKVKVPEKPLEVLRGIHSFDPCLACSTHLYNEKGEEIASVRVQGVVHV

Sequences:

>Translated_633_residues
MKRVVVDPVTRIEGHLRIEIMVDEETGQVKDALSAGTMWRGIELIVRNRDPRDVWAFTQRICGVCTSIHALASLRAVEDA
LEITIPKNANYIRNIMYGSLQVHDHVVHFYHLHALDWVSPVEALKADPVATAALANKILEKYGVLNEFMPDFLGHRAYPK
KFPKATPGYFREFQKKIKKLVESGQLGIFAAHWWDHPDYQMLPPEVHLIGIAHYLNMLDVQRELFIPQVVFGGKNPHPHY
IVGGVNCSISMDDMNAPVNAERLAVVEDAIYTQVESTDFFYIPDILAIADIYLNQHNWFYGGGLSKKRVIGYGDYPDEPY
TGIKNGDYHKKILWHSNGVVEDFYKGVEKAKFYNLEGKDFTDPEQIQEFVTHSWYKYPDETKGLHPWDGITEPNYTGPKE
GTKTHWKYLDENGKYSWIKAPRWRGKACEVGPLARYIIVYTKVKQGHIKPTWVDELIVNQIDTVSKILNLPPEKWLPTTV
GRTIARALEAQMSAHTNLYWMKKLYDNIKAGDTSVANMEKWDPSTWPKEAKGVGLTEAPRGALGHWVIIKDGKVANYQCV
VPTTWNGSPKDPKGQHGAFEESMIDTKVKVPEKPLEVLRGIHSFDPCLACSTHLYNEKGEEIASVRVQGVVHV
>Mature_633_residues
MKRVVVDPVTRIEGHLRIEIMVDEETGQVKDALSAGTMWRGIELIVRNRDPRDVWAFTQRICGVCTSIHALASLRAVEDA
LEITIPKNANYIRNIMYGSLQVHDHVVHFYHLHALDWVSPVEALKADPVATAALANKILEKYGVLNEFMPDFLGHRAYPK
KFPKATPGYFREFQKKIKKLVESGQLGIFAAHWWDHPDYQMLPPEVHLIGIAHYLNMLDVQRELFIPQVVFGGKNPHPHY
IVGGVNCSISMDDMNAPVNAERLAVVEDAIYTQVESTDFFYIPDILAIADIYLNQHNWFYGGGLSKKRVIGYGDYPDEPY
TGIKNGDYHKKILWHSNGVVEDFYKGVEKAKFYNLEGKDFTDPEQIQEFVTHSWYKYPDETKGLHPWDGITEPNYTGPKE
GTKTHWKYLDENGKYSWIKAPRWRGKACEVGPLARYIIVYTKVKQGHIKPTWVDELIVNQIDTVSKILNLPPEKWLPTTV
GRTIARALEAQMSAHTNLYWMKKLYDNIKAGDTSVANMEKWDPSTWPKEAKGVGLTEAPRGALGHWVIIKDGKVANYQCV
VPTTWNGSPKDPKGQHGAFEESMIDTKVKVPEKPLEVLRGIHSFDPCLACSTHLYNEKGEEIASVRVQGVVHV

Specific function: This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions [H]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787207, Length=630, Percent_Identity=48.5714285714286, Blast_Score=602, Evalue=1e-173,
Organism=Escherichia coli, GI1789368, Length=632, Percent_Identity=40.1898734177215, Blast_Score=400, Evalue=1e-112,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 71966; Mature: 71966

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: PS00507 NI_HGENASE_L_1 ; PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRVVVDPVTRIEGHLRIEIMVDEETGQVKDALSAGTMWRGIELIVRNRDPRDVWAFTQR
CCCEEECCHHHCCCEEEEEEEEECCCCHHHHHHHHCCHHHCEEEEEECCCCHHHHHHHHH
ICGVCTSIHALASLRAVEDALEITIPKNANYIRNIMYGSLQVHDHVVHFYHLHALDWVSP
HHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHCCEEHHHHHHHHHHHHHHHHHCH
VEALKADPVATAALANKILEKYGVLNEFMPDFLGHRAYPKKFPKATPGYFREFQKKIKKL
HHHHCCCCHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCHHCCCCCCHHHHHHHHHHHHH
VESGQLGIFAAHWWDHPDYQMLPPEVHLIGIAHYLNMLDVQRELFIPQVVFGGKNPHPHY
HHCCCCEEEEEECCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCHHEECCCCCCCCE
IVGGVNCSISMDDMNAPVNAERLAVVEDAIYTQVESTDFFYIPDILAIADIYLNQHNWFY
EEECEEEEEEECCCCCCCCHHHHHHHHHHHHHEECCCCEEECCHHHHHHHHHCCCCCEEE
GGGLSKKRVIGYGDYPDEPYTGIKNGDYHKKILWHSNGVVEDFYKGVEKAKFYNLEGKDF
CCCCCCCEEEECCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHEEECCCCCCC
TDPEQIQEFVTHSWYKYPDETKGLHPWDGITEPNYTGPKEGTKTHWKYLDENGKYSWIKA
CCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHEECCCCCEEEEEC
PRWRGKACEVGPLARYIIVYTKVKQGHIKPTWVDELIVNQIDTVSKILNLPPEKWLPTTV
CCCCCCCCCCCHHEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
GRTIARALEAQMSAHTNLYWMKKLYDNIKAGDTSVANMEKWDPSTWPKEAKGVGLTEAPR
HHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCC
GALGHWVIIKDGKVANYQCVVPTTWNGSPKDPKGQHGAFEESMIDTKVKVPEKPLEVLRG
CCCCEEEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHH
IHSFDPCLACSTHLYNEKGEEIASVRVQGVVHV
HHCCCHHHHHHHHHHCCCCHHHHHEEEEEEEEC
>Mature Secondary Structure
MKRVVVDPVTRIEGHLRIEIMVDEETGQVKDALSAGTMWRGIELIVRNRDPRDVWAFTQR
CCCEEECCHHHCCCEEEEEEEEECCCCHHHHHHHHCCHHHCEEEEEECCCCHHHHHHHHH
ICGVCTSIHALASLRAVEDALEITIPKNANYIRNIMYGSLQVHDHVVHFYHLHALDWVSP
HHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHCCEEHHHHHHHHHHHHHHHHHCH
VEALKADPVATAALANKILEKYGVLNEFMPDFLGHRAYPKKFPKATPGYFREFQKKIKKL
HHHHCCCCHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCHHCCCCCCHHHHHHHHHHHHH
VESGQLGIFAAHWWDHPDYQMLPPEVHLIGIAHYLNMLDVQRELFIPQVVFGGKNPHPHY
HHCCCCEEEEEECCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCHHEECCCCCCCCE
IVGGVNCSISMDDMNAPVNAERLAVVEDAIYTQVESTDFFYIPDILAIADIYLNQHNWFY
EEECEEEEEEECCCCCCCCHHHHHHHHHHHHHEECCCCEEECCHHHHHHHHHCCCCCEEE
GGGLSKKRVIGYGDYPDEPYTGIKNGDYHKKILWHSNGVVEDFYKGVEKAKFYNLEGKDF
CCCCCCCEEEECCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHEEECCCCCCC
TDPEQIQEFVTHSWYKYPDETKGLHPWDGITEPNYTGPKEGTKTHWKYLDENGKYSWIKA
CCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHEECCCCCEEEEEC
PRWRGKACEVGPLARYIIVYTKVKQGHIKPTWVDELIVNQIDTVSKILNLPPEKWLPTTV
CCCCCCCCCCCHHEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
GRTIARALEAQMSAHTNLYWMKKLYDNIKAGDTSVANMEKWDPSTWPKEAKGVGLTEAPR
HHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCC
GALGHWVIIKDGKVANYQCVVPTTWNGSPKDPKGQHGAFEESMIDTKVKVPEKPLEVLRG
CCCCEEEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHH
IHSFDPCLACSTHLYNEKGEEIASVRVQGVVHV
HHCCCHHHHHHHHHHCCCCHHHHHEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2402452; 2103457 [H]