Click here to switch to the map view.

The map label for this gene is def

Identifier: 15606030

GI number: 15606030

Start: 394092

End: 394601

Strand: Direct

Name: def

Synonym: aq_579

Alternate gene names: 15606030

Gene position: 394092-394601 (Clockwise)

Preceding gene: 15606028

Following gene: 15606031

Centisome position: 25.4

GC content: 46.67

Gene sequence:

>510_bases
ATGGTCAGAGACATAGTAATTTACCCCAACGAAATCTTGAAGAAACCGACCGAAAAAGTTGACGTAATAGATAAAGAGGT
TAAGAACCTCATAAGGGATATGTTCGATACCATGTACGAAGCGGAAGGTGTGGGACTCGCCGCAAACCAGATAGGTGTTC
CCTTGAGCGTTATGGTCATAGATACCTCACCCAAAGAAGACGCCCCTCCCCTCAAGCTCGTACTCATAAACCCCGAAATA
AAGGAAGGAGAGGGAAAGATTAAGTACAAGGAGGGATGTCTTTCCTTTCCGGGCCTCAGCGTTGAGGTAGAGCGTTTTCA
AAAGGTAAAGGTAAACGCTTTAAACGAACACGGAGAACCCGTTGAGTTAACTCTGGAGGGATTTCCAGCGATAGTTTTCC
AGCACGAGCTTGACCACCTGAAGGGCATAACTTTTGTGGACAGACTCAAGGGCTGGCGAAGGAGAATGGCACTCGAGAAG
TACCAGAAACTCCTCAAGTCCAGAAAATGA

Upstream 100 bases:

>100_bases
TATCCCGTGTAAACCTTTCCCCTCCAACCTTCTGCGAGCTCCTGTAAGTTTTGAACTTCCTTTATAAATTCTGAGAACTT
CATACCTTATATTTAATAGA

Downstream 100 bases:

>100_bases
GCACAAAGGAAATCATATGCACCCTTAAAGGGCAGTTTTTCCTCTCCCCGAGGGAAGAGAAGTTTTTAAGACTTCTGGAG
GAAATGGGCATACCAGAGGA

Product: polypeptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase

Number of amino acids: Translated: 169; Mature: 169

Protein sequence:

>169_residues
MVRDIVIYPNEILKKPTEKVDVIDKEVKNLIRDMFDTMYEAEGVGLAANQIGVPLSVMVIDTSPKEDAPPLKLVLINPEI
KEGEGKIKYKEGCLSFPGLSVEVERFQKVKVNALNEHGEPVELTLEGFPAIVFQHELDHLKGITFVDRLKGWRRRMALEK
YQKLLKSRK

Sequences:

>Translated_169_residues
MVRDIVIYPNEILKKPTEKVDVIDKEVKNLIRDMFDTMYEAEGVGLAANQIGVPLSVMVIDTSPKEDAPPLKLVLINPEI
KEGEGKIKYKEGCLSFPGLSVEVERFQKVKVNALNEHGEPVELTLEGFPAIVFQHELDHLKGITFVDRLKGWRRRMALEK
YQKLLKSRK
>Mature_169_residues
MVRDIVIYPNEILKKPTEKVDVIDKEVKNLIRDMFDTMYEAEGVGLAANQIGVPLSVMVIDTSPKEDAPPLKLVLINPEI
KEGEGKIKYKEGCLSFPGLSVEVERFQKVKVNALNEHGEPVELTLEGFPAIVFQHELDHLKGITFVDRLKGWRRRMALEK
YQKLLKSRK

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Homo sapiens, GI11641243, Length=151, Percent_Identity=30.4635761589404, Blast_Score=84, Evalue=8e-17,
Organism=Escherichia coli, GI1789682, Length=162, Percent_Identity=42.5925925925926, Blast_Score=127, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24645728, Length=155, Percent_Identity=34.8387096774194, Blast_Score=91, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24645726, Length=146, Percent_Identity=33.5616438356164, Blast_Score=80, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF_AQUAE (O66847)

Other databases:

- EMBL:   AE000657
- PIR:   C70352
- RefSeq:   NP_213407.1
- ProteinModelPortal:   O66847
- SMR:   O66847
- GeneID:   1193265
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_579
- NMPDR:   fig|224324.1.peg.422
- HOGENOM:   HBG665227
- OMA:   EFLAIVM
- PhylomeDB:   O66847
- ProtClustDB:   CLSK2299438
- BioCyc:   AAEO224324:AQ_579-MONOMER
- BRENDA:   3.5.1.88
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 19270; Mature: 19270

Theoretical pI: Translated: 7.59; Mature: 7.59

Prosite motif: NA

Important sites: ACT_SITE 136-136

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRDIVIYPNEILKKPTEKVDVIDKEVKNLIRDMFDTMYEAEGVGLAANQIGVPLSVMVI
CCCEEEECCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHCCCCCEEEEEE
DTSPKEDAPPLKLVLINPEIKEGEGKIKYKEGCLSFPGLSVEVERFQKVKVNALNEHGEP
ECCCCCCCCCEEEEEECCCCCCCCCCEEECCCCCCCCCCCEEHHHHHHHHHEECCCCCCC
VELTLEGFPAIVFQHELDHLKGITFVDRLKGWRRRMALEKYQKLLKSRK
EEEEECCCCEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVRDIVIYPNEILKKPTEKVDVIDKEVKNLIRDMFDTMYEAEGVGLAANQIGVPLSVMVI
CCCEEEECCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHCCCCCEEEEEE
DTSPKEDAPPLKLVLINPEIKEGEGKIKYKEGCLSFPGLSVEVERFQKVKVNALNEHGEP
ECCCCCCCCCEEEEEECCCCCCCCCCEEECCCCCCCCCCCEEHHHHHHHHHEECCCCCCC
VELTLEGFPAIVFQHELDHLKGITFVDRLKGWRRRMALEKYQKLLKSRK
EEEEECCCCEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320