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The map label for this gene is rfaE [H]

Identifier: 15606019

GI number: 15606019

Start: 385335

End: 386282

Strand: Direct

Name: rfaE [H]

Synonym: aq_565

Alternate gene names: 15606019

Gene position: 385335-386282 (Clockwise)

Preceding gene: 15606018

Following gene: 15606020

Centisome position: 24.84

GC content: 44.73

Gene sequence:

>948_bases
ATGCTATCCGAAGAAAGGGTAAAGGAACTTCTTAAAAAACTCAAAGATTTAAAAATCCTCGTGGTGGGAGACGTAATCCT
TGACACCTACCTTTACGGGAATGTGGAGAGGATTTCGCCCGAAGCTCCCGTTCCCGTTTTGGAAGTAGAAGGGGAAGAGT
TCAGACTGGGAGGAGCCGGAAACGTAGCGAAAAACTTATCTTCTTTAGGCGTGGAGACTTACCTTTGCGGTGTAGTAGGA
AAGGACGAAGCGGGGAAAAGATTAAGGGAATTAATAGAGGGAAATTCCATAAGGAGTTTTTTAATTGAGGACGAGAGACC
AACGACCAAGAAAACTAGGGTGGTATCGAGAAACCAGCAACTTCTTAGAATTGATTGGGAAGACAGGAGCTACATTTCCG
GAAAAGCCCTTGAGAAAATTCTGGACGTTATAAAGAACTTGCAGGTTGACGGGATAATCGTTTCAGACTACGCCAAGGGC
GTTATCACGGAAGAGGTAATAAAGACTATCAGAGAGAAAGGCGTTTTTTACTCAGTGGACCCGAGACCAAAGCACAGGGA
ACTCTACAGAAAAGCATATCTTATGACACCCAACGAAAAGGAATTGAGAGAAATGTCAAAATTTGAAGGAAGTGTTGAAG
AACTCGGTAAAAAATTAAAAGAGGAGCTGGAACTTGAAACGCTTATAGTTACCCGCGGGGAAAAAGGGATGAGTTTATTT
ACGGAGAAAGGAGTTAAGCACTTTCCAGCTAGGGCGAGAAAGGTTTACGATGTCACGGGAGCAGGGGACACGGTAATTGC
AAGCATAACAGCCTTTAAACTCGCGGGGGCTACATGGGAGGAGTGTTGTGAACTCGGAAACGTGTGCGGCGGTATAGTGG
TGGGTGAGTTTGGAACGGCAACCGTCACACCGGAAAAACTCCTGAAAGCCGTAAAACTAAATTCTTAA

Upstream 100 bases:

>100_bases
GCAGACGTTGCCCTCTACAAGGCTAAGGAAGAGGGGAGGAACAGGGTGGTCCGCTTCAAGCCCGAAATGTGGCAGGAGAA
GGAGTATTAAAATAATCCCG

Downstream 100 bases:

>100_bases
TTATGTTCAAAAACTTAATCTGGCTCAAAGAAGTAGACTCCACACAGGAAAGATTAAAGGAGTGGAACGTAAGCTACGGC
ACAGCTCTCGTTGCGGACAG

Product: ADP-heptose synthase

Products: NA

Alternate protein names: D-beta-D-heptose 7-phosphate kinase; D-beta-D-heptose 7-phosphotransferase; D-beta-D-heptose 1-phosphate adenosyltransferase [H]

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MLSEERVKELLKKLKDLKILVVGDVILDTYLYGNVERISPEAPVPVLEVEGEEFRLGGAGNVAKNLSSLGVETYLCGVVG
KDEAGKRLRELIEGNSIRSFLIEDERPTTKKTRVVSRNQQLLRIDWEDRSYISGKALEKILDVIKNLQVDGIIVSDYAKG
VITEEVIKTIREKGVFYSVDPRPKHRELYRKAYLMTPNEKELREMSKFEGSVEELGKKLKEELELETLIVTRGEKGMSLF
TEKGVKHFPARARKVYDVTGAGDTVIASITAFKLAGATWEECCELGNVCGGIVVGEFGTATVTPEKLLKAVKLNS

Sequences:

>Translated_315_residues
MLSEERVKELLKKLKDLKILVVGDVILDTYLYGNVERISPEAPVPVLEVEGEEFRLGGAGNVAKNLSSLGVETYLCGVVG
KDEAGKRLRELIEGNSIRSFLIEDERPTTKKTRVVSRNQQLLRIDWEDRSYISGKALEKILDVIKNLQVDGIIVSDYAKG
VITEEVIKTIREKGVFYSVDPRPKHRELYRKAYLMTPNEKELREMSKFEGSVEELGKKLKEELELETLIVTRGEKGMSLF
TEKGVKHFPARARKVYDVTGAGDTVIASITAFKLAGATWEECCELGNVCGGIVVGEFGTATVTPEKLLKAVKLNS
>Mature_315_residues
MLSEERVKELLKKLKDLKILVVGDVILDTYLYGNVERISPEAPVPVLEVEGEEFRLGGAGNVAKNLSSLGVETYLCGVVG
KDEAGKRLRELIEGNSIRSFLIEDERPTTKKTRVVSRNQQLLRIDWEDRSYISGKALEKILDVIKNLQVDGIIVSDYAKG
VITEEVIKTIREKGVFYSVDPRPKHRELYRKAYLMTPNEKELREMSKFEGSVEELGKKLKEELELETLIVTRGEKGMSLF
TEKGVKHFPARARKVYDVTGAGDTVIASITAFKLAGATWEECCELGNVCGGIVVGEFGTATVTPEKLLKAVKLNS

Specific function: Catalyzes the ADP transfer to D-glycero-D-manno-heptose 1-phosphate, yielding ADP-D,D-heptose [H]

COG id: COG2870

COG function: function code M; ADP-heptose synthase, bifunctional sugar kinase/adenylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the cytidylyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789432, Length=315, Percent_Identity=42.5396825396825, Blast_Score=221, Evalue=4e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023030
- InterPro:   IPR011611
- InterPro:   IPR004821
- InterPro:   IPR004820
- InterPro:   IPR011913
- InterPro:   IPR011914
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01467 CTP_transf_2; PF00294 PfkB [H]

EC number: 2.7.-.-

Molecular weight: Translated: 35108; Mature: 35108

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS00583 PFKB_KINASES_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSEERVKELLKKLKDLKILVVGDVILDTYLYGNVERISPEAPVPVLEVEGEEFRLGGAG
CCCHHHHHHHHHHHHCCEEEEEHHHHHHHHHHCCHHHCCCCCCCCEEEECCCEEEECCCC
NVAKNLSSLGVETYLCGVVGKDEAGKRLRELIEGNSIRSFLIEDERPTTKKTRVVSRNQQ
HHHHHHHHHCHHHHHCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHCCCE
LLRIDWEDRSYISGKALEKILDVIKNLQVDGIIVSDYAKGVITEEVIKTIREKGVFYSVD
EEEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEECCHHCCHHHHHHHHHHHHCCCEEECC
PRPKHRELYRKAYLMTPNEKELREMSKFEGSVEELGKKLKEELELETLIVTRGEKGMSLF
CCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEEEECCCCCHHHH
TEKGVKHFPARARKVYDVTGAGDTVIASITAFKLAGATWEECCELGNVCGGIVVGEFGTA
HHHCHHHCCHHHHEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEECCCCCC
TVTPEKLLKAVKLNS
CCCHHHHHHHHHCCC
>Mature Secondary Structure
MLSEERVKELLKKLKDLKILVVGDVILDTYLYGNVERISPEAPVPVLEVEGEEFRLGGAG
CCCHHHHHHHHHHHHCCEEEEEHHHHHHHHHHCCHHHCCCCCCCCEEEECCCEEEECCCC
NVAKNLSSLGVETYLCGVVGKDEAGKRLRELIEGNSIRSFLIEDERPTTKKTRVVSRNQQ
HHHHHHHHHCHHHHHCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHCCCE
LLRIDWEDRSYISGKALEKILDVIKNLQVDGIIVSDYAKGVITEEVIKTIREKGVFYSVD
EEEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEECCHHCCHHHHHHHHHHHHCCCEEECC
PRPKHRELYRKAYLMTPNEKELREMSKFEGSVEELGKKLKEELELETLIVTRGEKGMSLF
CCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEEEECCCCCHHHH
TEKGVKHFPARARKVYDVTGAGDTVIASITAFKLAGATWEECCELGNVCGGIVVGEFGTA
HHHCHHHCCHHHHEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEECCCCCC
TVTPEKLLKAVKLNS
CCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA