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The map label for this gene is phoB [H]

Identifier: 15605840

GI number: 15605840

Start: 207352

End: 208038

Strand: Direct

Name: phoB [H]

Synonym: aq_319

Alternate gene names: 15605840

Gene position: 207352-208038 (Clockwise)

Preceding gene: 15605835

Following gene: 15605841

Centisome position: 13.37

GC content: 42.94

Gene sequence:

>687_bases
ATGAGGAGGAAGATGGAAAGGATAGCTCTAATTGAGGACGACAAGGATTTAGCCTTTCTCGTGAAACTGAATTTAGAAAG
GGAGGGGTTTGAAGTAGAGCACTTTGAACGTGCCACTCCCTTCTTTAAGTTCATTTCTGAAAACAGTGTAGACCTTATCC
TCATAGACATTATGCTTCCCGACCTAGATGGATTCAGGATAGCTAACTTCTTAAAGAGCAGGGCGGATTTAAAAGAAATT
CCCGTTATATTCATAACCGCAAAAGGGGAGGAAGAGGACAAACTAAAGGGCTTTGAACTGGGAGCGGACGATTACATAAC
TAAACCCTTTTCCATGAAAGAGTTAATAGCAAGAGTAAGAGCCGTTCTAAAGAGGTATAAAAAGGTTTCCGAGAGCAAAG
TTTTAAAGTACGAGGGCGTTGAGCTGGACACGGAAAGACAAAAACTTTTCGTGGACGGTAGGGAGGTATACCTGACTCCT
GCGGAGTTTAAAATCCTGAAAACCTTGATGGAAAACTTCGGAAGACCTGTCAGTCGTTCGTCCCTCGTTGAAAAGCTCTG
GGATTACGAGAGGGAAACCACGGAGAGGGCTATAGACGTTCACGTAAAGCACATAAGAGACAAGCTCGGAAAGTATAAGG
GTCTTATAAAAACTGTGAGGGGTGTCGGATACAAGTTCGAAAATTAA

Upstream 100 bases:

>100_bases
GTAATTAGTAGGTTTTTGAAAAAGAGGAAGGTTGTAGCGGTTGTGAACAATCCCTCTCAGCTGAAGAACGATATTTTAAA
GAAAGCGGATGTTGTAATTT

Downstream 100 bases:

>100_bases
ACTTTTATTTATGTCCAAACTCAAGACTGAAAAAGAGTTGAAGAAACGGGAAATAATGAGGGTAGCCTGTAAACTCTTTG
CCCAGAAAGGCTATCACAAC

Product: transcriptional regulator (PhoB-like)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 228; Mature: 228

Protein sequence:

>228_residues
MRRKMERIALIEDDKDLAFLVKLNLEREGFEVEHFERATPFFKFISENSVDLILIDIMLPDLDGFRIANFLKSRADLKEI
PVIFITAKGEEEDKLKGFELGADDYITKPFSMKELIARVRAVLKRYKKVSESKVLKYEGVELDTERQKLFVDGREVYLTP
AEFKILKTLMENFGRPVSRSSLVEKLWDYERETTERAIDVHVKHIRDKLGKYKGLIKTVRGVGYKFEN

Sequences:

>Translated_228_residues
MRRKMERIALIEDDKDLAFLVKLNLEREGFEVEHFERATPFFKFISENSVDLILIDIMLPDLDGFRIANFLKSRADLKEI
PVIFITAKGEEEDKLKGFELGADDYITKPFSMKELIARVRAVLKRYKKVSESKVLKYEGVELDTERQKLFVDGREVYLTP
AEFKILKTLMENFGRPVSRSSLVEKLWDYERETTERAIDVHVKHIRDKLGKYKGLIKTVRGVGYKFEN
>Mature_228_residues
MRRKMERIALIEDDKDLAFLVKLNLEREGFEVEHFERATPFFKFISENSVDLILIDIMLPDLDGFRIANFLKSRADLKEI
PVIFITAKGEEEDKLKGFELGADDYITKPFSMKELIARVRAVLKRYKKVSESKVLKYEGVELDTERQKLFVDGREVYLTP
AEFKILKTLMENFGRPVSRSSLVEKLWDYERETTERAIDVHVKHIRDKLGKYKGLIKTVRGVGYKFEN

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=224, Percent_Identity=36.6071428571429, Blast_Score=161, Evalue=4e-41,
Organism=Escherichia coli, GI1790860, Length=225, Percent_Identity=37.7777777777778, Blast_Score=150, Evalue=5e-38,
Organism=Escherichia coli, GI1788394, Length=225, Percent_Identity=36.8888888888889, Blast_Score=141, Evalue=3e-35,
Organism=Escherichia coli, GI1790552, Length=220, Percent_Identity=33.1818181818182, Blast_Score=138, Evalue=3e-34,
Organism=Escherichia coli, GI1789809, Length=229, Percent_Identity=34.4978165938865, Blast_Score=135, Evalue=2e-33,
Organism=Escherichia coli, GI1786784, Length=223, Percent_Identity=34.9775784753363, Blast_Score=135, Evalue=2e-33,
Organism=Escherichia coli, GI1789402, Length=220, Percent_Identity=35, Blast_Score=134, Evalue=4e-33,
Organism=Escherichia coli, GI87082012, Length=222, Percent_Identity=30.6306306306306, Blast_Score=124, Evalue=7e-30,
Organism=Escherichia coli, GI2367329, Length=233, Percent_Identity=35.1931330472103, Blast_Score=121, Evalue=4e-29,
Organism=Escherichia coli, GI1787375, Length=223, Percent_Identity=31.390134529148, Blast_Score=119, Evalue=1e-28,
Organism=Escherichia coli, GI1786911, Length=229, Percent_Identity=29.6943231441048, Blast_Score=117, Evalue=6e-28,
Organism=Escherichia coli, GI1790863, Length=234, Percent_Identity=32.0512820512821, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1787229, Length=224, Percent_Identity=30.3571428571429, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI145693140, Length=238, Percent_Identity=26.4705882352941, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1790299, Length=141, Percent_Identity=24.822695035461, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26730; Mature: 26730

Theoretical pI: Translated: 9.13; Mature: 9.13

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRKMERIALIEDDKDLAFLVKLNLEREGFEVEHFERATPFFKFISENSVDLILIDIMLP
CCCCHHHEEEEECCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECC
DLDGFRIANFLKSRADLKEIPVIFITAKGEEEDKLKGFELGADDYITKPFSMKELIARVR
CCCCHHHHHHHHHHCCHHHCCEEEEEECCCCCHHCCCEECCCCCCCCCCCCHHHHHHHHH
AVLKRYKKVSESKVLKYEGVELDTERQKLFVDGREVYLTPAEFKILKTLMENFGRPVSRS
HHHHHHHHHHHHHEEEECCCCCCCCHHEEEECCCEEEECHHHHHHHHHHHHHHCCCCCHH
SLVEKLWDYERETTERAIDVHVKHIRDKLGKYKGLIKTVRGVGYKFEN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRRKMERIALIEDDKDLAFLVKLNLEREGFEVEHFERATPFFKFISENSVDLILIDIMLP
CCCCHHHEEEEECCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECC
DLDGFRIANFLKSRADLKEIPVIFITAKGEEEDKLKGFELGADDYITKPFSMKELIARVR
CCCCHHHHHHHHHHCCHHHCCEEEEEECCCCCHHCCCEECCCCCCCCCCCCHHHHHHHHH
AVLKRYKKVSESKVLKYEGVELDTERQKLFVDGREVYLTPAEFKILKTLMENFGRPVSRS
HHHHHHHHHHHHHEEEECCCCCCCCHHEEEECCCEEEECHHHHHHHHHHHHHHCCCCCHH
SLVEKLWDYERETTERAIDVHVKHIRDKLGKYKGLIKTVRGVGYKFEN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]