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The map label for this gene is aspC4

Identifier: 15605813

GI number: 15605813

Start: 176409

End: 177572

Strand: Direct

Name: aspC4

Synonym: aq_273

Alternate gene names: 15605813

Gene position: 176409-177572 (Clockwise)

Preceding gene: 15605811

Following gene: 15605814

Centisome position: 11.37

GC content: 46.48

Gene sequence:

>1164_bases
GTGTTTGAGTTTTCGGACAGGTTAAAGGTTCTCCCGCCTTACCTTTTTGCAGAACTCGATAGGAAGAAACAGGAGAAGAT
AGAACAGGGTGTGGACGTTATAGACCTCGGTGTGGGAGACCCGGACATGCCCACACCAAAACCCATAGTTGAAGCTGCAA
AAAAAGCCTTAGAAAATCCTGAAAATCACAAGTACCCTTCTTACGTAGGGAAGTACGAGTTCAGGAAGGCGGTTGCAGAC
TGGTACAAGAGAAGGTTTGACGTTGATCTTGATCCAAACACCGAAGTTATAACCCTTATCGGTTCAAAGGAAGGGATAGC
ACACTTTCCCTTAGCCTTCGTAAATCCCGGAGACATAGTTCTCTGTCCAGACCCTGCGTATCCCGTTTACAGAATTGGAG
CAATATTTGCCGGAGGAACTCCATACACGGTTCCCTTAAAAGAAGAGAACAACTTCCTCCCCGACCTTGATTCAATTCCA
GAAGACGTGGCGAAAAAGGCAAAGATTATATGGATTAATTACCCTAATAACCCCACTTCCGCACCACCTACCCTTGAGTT
TTACAAAAAACTCGTTGACTGGGCTAAGGAGTACAACGTTATAATAGCCTCCGACAACGCTTACTCCGAGATTTATACGG
GACAGGAAAAGCCCCCTTCCATACTCCAAGTTCCCGGAGCCAAAGACGTAGCCATAGAGTTTCACTCCCTCTCCAAAACT
TACAATATGACGGGATGGCGTATAGGCATGGCTGTAGGGAATAAGGAACTCGTTGCGGGATTAGGAAAGGTAAAAACGAA
CGTGGACAGCGGTCAGTTCGGAGCTGTTCAGGATGCGGGAATAGTAGCCCTGAACCTGCCCGAAGAAGAGGTGGAAAAGA
TAAGGGACGTTTACAGGGAAAGAAAGAAGATAATGACGGAAGCCCTTGAAAAGATAGGACTCGAGATTTACAGGAGCGAT
TACACCTTCTACCTCTGGATAAAAGTACCGGAAGGATACACTTCCGCAGAGTTCGTGGGAAGACTCATAGACGAGGCGGG
TATAGTTTGCACCCCCGGAAACGGTTTCGGAGAGTACGGAGAGGGTTATTTTAGGATTTCCTTAACAGTTCCCACCGAGA
GACTCCTTGAGGCTGCGGAAAGAATTAAAAATTTAAAACTGTAA

Upstream 100 bases:

>100_bases
GTGCTCAAATCAAGCTTCACCTTTCTCCTCCTTTATTTAAGATAAGTGTAACTCTTACATACTGATATAATCTTCTAAAA
ACTTTAAAGGAGGTAGCGGA

Downstream 100 bases:

>100_bases
TCTCCCTTTCTCCTTTTCCTTTCTTTTAAGATTAAATCTTATGAACGCCTGTGAGAGGTTATCCCGCGTCCTTGAGAGAA
TTCAGAAAGCCTGCGAGAGA

Product: LL-diaminopimelate aminotransferase

Products: NA

Alternate protein names: DAP-AT; DAP-aminotransferase; LL-DAP-aminotransferase

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MFEFSDRLKVLPPYLFAELDRKKQEKIEQGVDVIDLGVGDPDMPTPKPIVEAAKKALENPENHKYPSYVGKYEFRKAVAD
WYKRRFDVDLDPNTEVITLIGSKEGIAHFPLAFVNPGDIVLCPDPAYPVYRIGAIFAGGTPYTVPLKEENNFLPDLDSIP
EDVAKKAKIIWINYPNNPTSAPPTLEFYKKLVDWAKEYNVIIASDNAYSEIYTGQEKPPSILQVPGAKDVAIEFHSLSKT
YNMTGWRIGMAVGNKELVAGLGKVKTNVDSGQFGAVQDAGIVALNLPEEEVEKIRDVYRERKKIMTEALEKIGLEIYRSD
YTFYLWIKVPEGYTSAEFVGRLIDEAGIVCTPGNGFGEYGEGYFRISLTVPTERLLEAAERIKNLKL

Sequences:

>Translated_387_residues
MFEFSDRLKVLPPYLFAELDRKKQEKIEQGVDVIDLGVGDPDMPTPKPIVEAAKKALENPENHKYPSYVGKYEFRKAVAD
WYKRRFDVDLDPNTEVITLIGSKEGIAHFPLAFVNPGDIVLCPDPAYPVYRIGAIFAGGTPYTVPLKEENNFLPDLDSIP
EDVAKKAKIIWINYPNNPTSAPPTLEFYKKLVDWAKEYNVIIASDNAYSEIYTGQEKPPSILQVPGAKDVAIEFHSLSKT
YNMTGWRIGMAVGNKELVAGLGKVKTNVDSGQFGAVQDAGIVALNLPEEEVEKIRDVYRERKKIMTEALEKIGLEIYRSD
YTFYLWIKVPEGYTSAEFVGRLIDEAGIVCTPGNGFGEYGEGYFRISLTVPTERLLEAAERIKNLKL
>Mature_387_residues
MFEFSDRLKVLPPYLFAELDRKKQEKIEQGVDVIDLGVGDPDMPTPKPIVEAAKKALENPENHKYPSYVGKYEFRKAVAD
WYKRRFDVDLDPNTEVITLIGSKEGIAHFPLAFVNPGDIVLCPDPAYPVYRIGAIFAGGTPYTVPLKEENNFLPDLDSIP
EDVAKKAKIIWINYPNNPTSAPPTLEFYKKLVDWAKEYNVIIASDNAYSEIYTGQEKPPSILQVPGAKDVAIEFHSLSKT
YNMTGWRIGMAVGNKELVAGLGKVKTNVDSGQFGAVQDAGIVALNLPEEEVEKIRDVYRERKKIMTEALEKIGLEIYRSD
YTFYLWIKVPEGYTSAEFVGRLIDEAGIVCTPGNGFGEYGEGYFRISLTVPTERLLEAAERIKNLKL

Specific function: Involved in the synthesis of meso-diaminopimelate (m-DAP or DL-DAP), required for both lysine and peptidoglycan biosynthesis. Catalyzes the direct conversion of tetrahydrodipicolinate to LL-diaminopimelate, a reaction that requires three enzymes in E.coli

COG id: COG0436

COG function: function code E; Aspartate/tyrosine/aromatic aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. LL-diaminopimelate aminotransferase subfamily

Homologues:

Organism=Homo sapiens, GI56713254, Length=302, Percent_Identity=25.1655629139073, Blast_Score=96, Evalue=7e-20,
Organism=Homo sapiens, GI56713256, Length=302, Percent_Identity=25.1655629139073, Blast_Score=96, Evalue=8e-20,
Organism=Homo sapiens, GI4507369, Length=373, Percent_Identity=26.2734584450402, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI95147551, Length=321, Percent_Identity=23.6760124610592, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI169881279, Length=321, Percent_Identity=23.6760124610592, Blast_Score=89, Evalue=6e-18,
Organism=Escherichia coli, GI1788722, Length=382, Percent_Identity=40.0523560209424, Blast_Score=306, Evalue=2e-84,
Organism=Escherichia coli, GI1786816, Length=367, Percent_Identity=24.7956403269755, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1788627, Length=231, Percent_Identity=31.1688311688312, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788332, Length=241, Percent_Identity=27.3858921161826, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17567369, Length=339, Percent_Identity=24.7787610619469, Blast_Score=114, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI71994472, Length=244, Percent_Identity=29.5081967213115, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71994476, Length=244, Percent_Identity=29.5081967213115, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17567663, Length=373, Percent_Identity=26.5415549597855, Blast_Score=96, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6322401, Length=391, Percent_Identity=25.8312020460358, Blast_Score=106, Evalue=7e-24,
Organism=Drosophila melanogaster, GI28573069, Length=389, Percent_Identity=23.3933161953728, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24646114, Length=389, Percent_Identity=23.3933161953728, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI28573067, Length=389, Percent_Identity=23.3933161953728, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI28573065, Length=389, Percent_Identity=23.3933161953728, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPAT_AQUAE (O66630)

Other databases:

- EMBL:   AE000657
- PIR:   B70325
- RefSeq:   NP_213190.1
- HSSP:   Q9X0Y2
- ProteinModelPortal:   O66630
- SMR:   O66630
- GeneID:   1192864
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_273
- NMPDR:   fig|224324.1.peg.205
- HOGENOM:   HBG645860
- OMA:   YSEIAFD
- PhylomeDB:   O66630
- ProtClustDB:   PRK09276
- BioCyc:   AAEO224324:AQ_273-MONOMER
- HAMAP:   MF_01642
- InterPro:   IPR004839
- InterPro:   IPR019881
- InterPro:   IPR004838
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- TIGRFAMs:   TIGR03540

Pfam domain/function: PF00155 Aminotran_1_2; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.6.1.83

Molecular weight: Translated: 43444; Mature: 43444

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00105 AA_TRANSFER_CLASS_1

Important sites: BINDING 39-39 BINDING 65-65 BINDING 127-127 BINDING 177-177 BINDING 177-177 BINDING 205-205 BINDING 208-208 BINDING 236-236 BINDING 238-238 BINDING 247-247 BINDING 365-365

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFEFSDRLKVLPPYLFAELDRKKQEKIEQGVDVIDLGVGDPDMPTPKPIVEAAKKALENP
CCCCCCCCHHCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHCCC
ENHKYPSYVGKYEFRKAVADWYKRRFDVDLDPNTEVITLIGSKEGIAHFPLAFVNPGDIV
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCEECCEEEECCCCEE
LCPDPAYPVYRIGAIFAGGTPYTVPLKEENNFLPDLDSIPEDVAKKAKIIWINYPNNPTS
ECCCCCCCCEEEEEEEECCCEEEEEECCCCCCCCCHHCCHHHHHCCEEEEEEECCCCCCC
APPTLEFYKKLVDWAKEYNVIIASDNAYSEIYTGQEKPPSILQVPGAKDVAIEFHSLSKT
CCCHHHHHHHHHHHHHHCCEEEECCCCHHHHHCCCCCCCCEEECCCCCEEEEEEEHHCCC
YNMTGWRIGMAVGNKELVAGLGKVKTNVDSGQFGAVQDAGIVALNLPEEEVEKIRDVYRE
CCCCCEEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHH
RKKIMTEALEKIGLEIYRSDYTFYLWIKVPEGYTSAEFVGRLIDEAGIVCTPGNGFGEYG
HHHHHHHHHHHHCCEEEECCCEEEEEEECCCCCCHHHHHHHHHHCCCEEEECCCCCCCCC
EGYFRISLTVPTERLLEAAERIKNLKL
CCEEEEEEECCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MFEFSDRLKVLPPYLFAELDRKKQEKIEQGVDVIDLGVGDPDMPTPKPIVEAAKKALENP
CCCCCCCCHHCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHCCC
ENHKYPSYVGKYEFRKAVADWYKRRFDVDLDPNTEVITLIGSKEGIAHFPLAFVNPGDIV
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCEECCEEEECCCCEE
LCPDPAYPVYRIGAIFAGGTPYTVPLKEENNFLPDLDSIPEDVAKKAKIIWINYPNNPTS
ECCCCCCCCEEEEEEEECCCEEEEEECCCCCCCCCHHCCHHHHHCCEEEEEEECCCCCCC
APPTLEFYKKLVDWAKEYNVIIASDNAYSEIYTGQEKPPSILQVPGAKDVAIEFHSLSKT
CCCHHHHHHHHHHHHHHCCEEEECCCCHHHHHCCCCCCCCEEECCCCCEEEEEEEHHCCC
YNMTGWRIGMAVGNKELVAGLGKVKTNVDSGQFGAVQDAGIVALNLPEEEVEKIRDVYRE
CCCCCEEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHH
RKKIMTEALEKIGLEIYRSDYTFYLWIKVPEGYTSAEFVGRLIDEAGIVCTPGNGFGEYG
HHHHHHHHHHHHCCEEEECCCEEEEEEECCCCCCHHHHHHHHHHCCCEEEECCCCCCCCC
EGYFRISLTVPTERLLEAAERIKNLKL
CCEEEEEEECCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320