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The map label for this gene is folE

Identifier: 15605786

GI number: 15605786

Start: 149720

End: 150274

Strand: Direct

Name: folE

Synonym: aq_239

Alternate gene names: 15605786

Gene position: 149720-150274 (Clockwise)

Preceding gene: 15605785

Following gene: 15605788

Centisome position: 9.65

GC content: 45.23

Gene sequence:

>555_bases
ATGGCTATCGATAAGGAAAAGATCAAGCAGGCTGTAAGACTGTTTTTGGAAGGAATAGGAGAAGATCCCGACAGGGAAGG
ACTGAAAGAGACCCCCGAACGCGTTGCGAGAATGTGGGAAGAGTTTGAGAGGATGAGAAACTTTGACATGAAGTTATTTG
AGGAGTTCGGGGGATACAACGAAATGGTTCTCGTAAAAGACATAAAGTTTTACAGTCTATGTGAGCACCATCTTTTACCT
TTCTTCGGTAAAGTTCACATAGCGTACATTCCCGATAAAAAAATCTCCGGACTTTCCAAACTCGTGAGAACGGTGAGAGC
TTTTGCCCTGAGACCGCAGGTTCAAGAGAGGCTAACCGAAGAGATAGCGGACTTTCTGGAAAAGGAACTAGAACCCAAAG
GTGTGGGAGTAGTTATAGAGGCAGAACACCTCTGTATGTCCATGAGAGGGGTTATGTCTCCCGGACACCTCACGGTCACC
TCAGCCCTGAGGGGTGTGTTTTTAAAGGACATAAAAACGAGGGAAGAGTTTTTAAAGCTCGTTAAAGGCGTTTAA

Upstream 100 bases:

>100_bases
CCTGCGGGGTGATAAGGTAATTTCCTTTCCTCTTTATCTTTTAAACTTTTGTATGACCCAAATTCTCAAACTTCTTTTCC
CGAAAATTTAAAATTTTCCT

Downstream 100 bases:

>100_bases
GGTATTGGAACCCTGCTTATGACTTCCTTTAAGACCGCTTCCGCCTTCACTCCTTCCCTTACCTTTATCCTGTGGGGAAT
TACGTACTTAAGTAAGTCCA

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I

Number of amino acids: Translated: 184; Mature: 183

Protein sequence:

>184_residues
MAIDKEKIKQAVRLFLEGIGEDPDREGLKETPERVARMWEEFERMRNFDMKLFEEFGGYNEMVLVKDIKFYSLCEHHLLP
FFGKVHIAYIPDKKISGLSKLVRTVRAFALRPQVQERLTEEIADFLEKELEPKGVGVVIEAEHLCMSMRGVMSPGHLTVT
SALRGVFLKDIKTREEFLKLVKGV

Sequences:

>Translated_184_residues
MAIDKEKIKQAVRLFLEGIGEDPDREGLKETPERVARMWEEFERMRNFDMKLFEEFGGYNEMVLVKDIKFYSLCEHHLLP
FFGKVHIAYIPDKKISGLSKLVRTVRAFALRPQVQERLTEEIADFLEKELEPKGVGVVIEAEHLCMSMRGVMSPGHLTVT
SALRGVFLKDIKTREEFLKLVKGV
>Mature_183_residues
AIDKEKIKQAVRLFLEGIGEDPDREGLKETPERVARMWEEFERMRNFDMKLFEEFGGYNEMVLVKDIKFYSLCEHHLLPF
FGKVHIAYIPDKKISGLSKLVRTVRAFALRPQVQERLTEEIADFLEKELEPKGVGVVIEAEHLCMSMRGVMSPGHLTVTS
ALRGVFLKDIKTREEFLKLVKGV

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family

Homologues:

Organism=Homo sapiens, GI66932968, Length=183, Percent_Identity=47.5409836065574, Blast_Score=168, Evalue=2e-42,
Organism=Homo sapiens, GI4503949, Length=183, Percent_Identity=47.5409836065574, Blast_Score=168, Evalue=2e-42,
Organism=Homo sapiens, GI66932970, Length=163, Percent_Identity=42.3312883435583, Blast_Score=124, Evalue=6e-29,
Organism=Homo sapiens, GI66932972, Length=141, Percent_Identity=45.3900709219858, Blast_Score=123, Evalue=1e-28,
Organism=Escherichia coli, GI1788476, Length=162, Percent_Identity=42.5925925925926, Blast_Score=115, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17560486, Length=179, Percent_Identity=50.2793296089386, Blast_Score=177, Evalue=3e-45,
Organism=Saccharomyces cerevisiae, GI6321706, Length=183, Percent_Identity=49.7267759562842, Blast_Score=169, Evalue=3e-43,
Organism=Drosophila melanogaster, GI24656782, Length=181, Percent_Identity=50.2762430939227, Blast_Score=165, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24656791, Length=182, Percent_Identity=50, Blast_Score=164, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24656796, Length=182, Percent_Identity=50, Blast_Score=164, Evalue=3e-41,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): GCH1_AQUAE (O66603)

Other databases:

- EMBL:   AE000657
- PIR:   G70321
- RefSeq:   NP_213163.1
- ProteinModelPortal:   O66603
- SMR:   O66603
- GeneID:   1192837
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_239
- NMPDR:   fig|224324.1.peg.178
- HOGENOM:   HBG370191
- OMA:   VTQTQAE
- PhylomeDB:   O66603
- ProtClustDB:   PRK09347
- BioCyc:   AAEO224324:AQ_239-MONOMER
- BRENDA:   3.5.4.16
- GO:   GO:0005737
- HAMAP:   MF_00223
- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234
- TIGRFAMs:   TIGR00063

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =3.5.4.16

Molecular weight: Translated: 21285; Mature: 21154

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIDKEKIKQAVRLFLEGIGEDPDREGLKETPERVARMWEEFERMRNFDMKLFEEFGGYN
CCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCC
EMVLVKDIKFYSLCEHHLLPFFGKVHIAYIPDKKISGLSKLVRTVRAFALRPQVQERLTE
CEEEEEHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHCHHHHHHHHH
EIADFLEKELEPKGVGVVIEAEHLCMSMRGVMSPGHLTVTSALRGVFLKDIKTREEFLKL
HHHHHHHHHCCCCCCEEEEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VKGV
HHCC
>Mature Secondary Structure 
AIDKEKIKQAVRLFLEGIGEDPDREGLKETPERVARMWEEFERMRNFDMKLFEEFGGYN
CCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCC
EMVLVKDIKFYSLCEHHLLPFFGKVHIAYIPDKKISGLSKLVRTVRAFALRPQVQERLTE
CEEEEEHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHCHHHHHHHHH
EIADFLEKELEPKGVGVVIEAEHLCMSMRGVMSPGHLTVTSALRGVFLKDIKTREEFLKL
HHHHHHHHHCCCCCCEEEEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VKGV
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320