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The map label for this gene is hemK

Identifier: 15605689

GI number: 15605689

Start: 61934

End: 62779

Strand: Direct

Name: hemK

Synonym: aq_099

Alternate gene names: 15605689

Gene position: 61934-62779 (Clockwise)

Preceding gene: 15605688

Following gene: 15605690

Centisome position: 3.99

GC content: 42.67

Gene sequence:

>846_bases
TTGAGAAAAGTGTTCAAACTGAACAAAGTGAAGGTAAAGGAACTACTCAAGAAAGTGGAAAGTGAGTACAGGAGAGACGC
GGAAATAATACTCTCCTACCTTTTGAAAGTTTCTCCCTCCCAGATTCCCCTGATGTATGCGAGAGAAATTCCTGAGGAGA
TTGTAAAAAGATTCTTCAAACAAATGAAGGAAAGAAAAAAAGGTATTCCCACAGCCTACGTTATCGGGGAGTGGGAGTGT
ATGGGAAGAGTTTTCAAAGTGAAAAAGGGCGTTCTCGTTCCAAGACCGGAAACGGAAATACTTATAGAAAGGACCTTAGA
GTTGATTCCACAAGACAGAGAAATGGTGGGGTTTGAGCTTGGAAGCGGAACGGGTTGTATATCCATTAATCTGTTAATTG
AAAGGCCGAAGCTAGTAATGTACGCCACAGATGTAAACCCCGATGCGGTGGAGCTCACAAAGGAAAACGCCAAACTCCAC
AAAGTTGACGACAGGCTTTTCGTTTTCTTGGGAAACGCCTTTGAACCCGTTAAAGGCATGAAGTTCGACTTCATAGTCTC
AAATCCCCCATACATACCCGAAAACTTCTGGGAAATTCTTCCAGAAGAAGTAAAAAAGGAGGGCTACACTTCTCTCATAG
GAGGCAAAAAGGGGTGGGAGTTTTACGAACTGATTGCGGAAGAGGGAACTAAACACCTGAAAGAGAATGGGTTTATAGCC
CTTGAGATAGGACACGACCAGGGAAAAGTTGTTAAGGAACTCCTTGAAAAGAAATGTTTTAAAGTTAATATTTTTAAGGA
CTACGCTGGATTTGACAGGGTGGTGATAGCACAGAGATGGAGCTGA

Upstream 100 bases:

>100_bases
GTTGAGACTATCCTCACAAAAGCCACTTACTGGCTTGGAGCACTCTTTTTAGTCCTTGCACTCCTTCTTTCGGTCATTCC
AAAGGAAAAGGGTTCTGTAG

Downstream 100 bases:

>100_bases
TAGGTTTTCTAATAGGAGTTTTCTTCTTCATAATCCTTGAGGGATTTTTTGCAGGGTCAGAGATAGCTCTAGTGAACGCT
GACAAAGGCTTTTTAAAGGC

Product: protoporphyrinogen oxidase

Products: NA

Alternate protein names: M.AaoHemKP

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MRKVFKLNKVKVKELLKKVESEYRRDAEIILSYLLKVSPSQIPLMYAREIPEEIVKRFFKQMKERKKGIPTAYVIGEWEC
MGRVFKVKKGVLVPRPETEILIERTLELIPQDREMVGFELGSGTGCISINLLIERPKLVMYATDVNPDAVELTKENAKLH
KVDDRLFVFLGNAFEPVKGMKFDFIVSNPPYIPENFWEILPEEVKKEGYTSLIGGKKGWEFYELIAEEGTKHLKENGFIA
LEIGHDQGKVVKELLEKKCFKVNIFKDYAGFDRVVIAQRWS

Sequences:

>Translated_281_residues
MRKVFKLNKVKVKELLKKVESEYRRDAEIILSYLLKVSPSQIPLMYAREIPEEIVKRFFKQMKERKKGIPTAYVIGEWEC
MGRVFKVKKGVLVPRPETEILIERTLELIPQDREMVGFELGSGTGCISINLLIERPKLVMYATDVNPDAVELTKENAKLH
KVDDRLFVFLGNAFEPVKGMKFDFIVSNPPYIPENFWEILPEEVKKEGYTSLIGGKKGWEFYELIAEEGTKHLKENGFIA
LEIGHDQGKVVKELLEKKCFKVNIFKDYAGFDRVVIAQRWS
>Mature_281_residues
MRKVFKLNKVKVKELLKKVESEYRRDAEIILSYLLKVSPSQIPLMYAREIPEEIVKRFFKQMKERKKGIPTAYVIGEWEC
MGRVFKVKKGVLVPRPETEILIERTLELIPQDREMVGFELGSGTGCISINLLIERPKLVMYATDVNPDAVELTKENAKLH
KVDDRLFVFLGNAFEPVKGMKFDFIVSNPPYIPENFWEILPEEVKKEGYTSLIGGKKGWEFYELIAEEGTKHLKENGFIA
LEIGHDQGKVVKELLEKKCFKVNIFKDYAGFDRVVIAQRWS

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family

Homologues:

Organism=Homo sapiens, GI7705409, Length=277, Percent_Identity=27.7978339350181, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1787463, Length=264, Percent_Identity=31.0606060606061, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI87082085, Length=194, Percent_Identity=28.8659793814433, Blast_Score=90, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24582226, Length=255, Percent_Identity=29.4117647058824, Blast_Score=108, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEMK_AQUAE (O66506)

Other databases:

- EMBL:   AE000657
- PIR:   G70309
- RefSeq:   NP_213065.1
- ProteinModelPortal:   O66506
- SMR:   O66506
- GeneID:   1192517
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_099
- NMPDR:   fig|224324.1.peg.80
- HOGENOM:   HBG732041
- OMA:   DPRPETE
- PhylomeDB:   O66506
- ProtClustDB:   CLSK2748244
- BioCyc:   AAEO224324:AQ_099-MONOMER
- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848
- TIGRFAMs:   TIGR00536
- TIGRFAMs:   TIGR03534

Pfam domain/function: PF05175 MTS

EC number: 2.1.1.-

Molecular weight: Translated: 32578; Mature: 32578

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: PS00092 N6_MTASE

Important sites: BINDING 144-144 BINDING 188-188

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKVFKLNKVKVKELLKKVESEYRRDAEIILSYLLKVSPSQIPLMYAREIPEEIVKRFFK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
QMKERKKGIPTAYVIGEWECMGRVFKVKKGVLVPRPETEILIERTLELIPQDREMVGFEL
HHHHHHCCCCEEEEEECHHHHHHHHHHHCCEECCCCCHHHHHHHHHHHCCCCCHHEEEEE
GSGTGCISINLLIERPKLVMYATDVNPDAVELTKENAKLHKVDDRLFVFLGNAFEPVKGM
CCCCCEEEEEEEEECCEEEEEEECCCCCCEEEECCCCEEEEECCEEEEEECCCCCCCCCC
KFDFIVSNPPYIPENFWEILPEEVKKEGYTSLIGGKKGWEFYELIAEEGTKHLKENGFIA
EEEEEECCCCCCCHHHHHHCCHHHHHCCCHHHHCCCCCHHHHHHHHHHHHHHHHHCCEEE
LEIGHDQGKVVKELLEKKCFKVNIFKDYAGFDRVVIAQRWS
EEECCCCCHHHHHHHHHHCEEEEEEHHHCCCCEEEEEECCC
>Mature Secondary Structure
MRKVFKLNKVKVKELLKKVESEYRRDAEIILSYLLKVSPSQIPLMYAREIPEEIVKRFFK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
QMKERKKGIPTAYVIGEWECMGRVFKVKKGVLVPRPETEILIERTLELIPQDREMVGFEL
HHHHHHCCCCEEEEEECHHHHHHHHHHHCCEECCCCCHHHHHHHHHHHCCCCCHHEEEEE
GSGTGCISINLLIERPKLVMYATDVNPDAVELTKENAKLHKVDDRLFVFLGNAFEPVKGM
CCCCCEEEEEEEEECCEEEEEEECCCCCCEEEECCCCEEEEECCEEEEEECCCCCCCCCC
KFDFIVSNPPYIPENFWEILPEEVKKEGYTSLIGGKKGWEFYELIAEEGTKHLKENGFIA
EEEEEECCCCCCCHHHHHHCCHHHHHCCCHHHHCCCCCHHHHHHHHHHHHHHHHHCCEEE
LEIGHDQGKVVKELLEKKCFKVNIFKDYAGFDRVVIAQRWS
EEECCCCCHHHHHHHHHHCEEEEEEHHHCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320