BacMap

Definition	Aquifex aeolicus VF5, complete genome.
Accession	NC_000918
Length	1,551,335

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The map label for this gene is tufA1

Identifier: 15605614

GI number: 15605614

Start: 2117

End: 3334

Strand: Direct

Name: tufA1

Synonym: aq_005

Alternate gene names: 15605614

Gene position: 2117-3334 (Clockwise)

Preceding gene: 15605613

Following gene: 15605615

Centisome position: 0.14

GC content: 49.59

Gene sequence:

>1218_bases
ATGGCAAAGGAGAAATTTGAAAGGACAAAAGAACACGTAAACGTGGGAACAATAGGACACGTTGACCACGGCAAAAGCAC
TCTCACTTCCGCTATAACATGCGTTCTCGCTGCAGGCCTCGTTGAAGGCGGTAAAGCTAAGTGCTTCAAATACGAAGAAA
TCGACAAGGCTCCAGAAGAAAAAGAAAGAGGAATCACGATAAACATCACCCACGTTGAGTACGAAACCGCCAAACGCCAC
TACGCTCACGTTGACTGCCCCGGTCACGCTGACTACATCAAGAATATGATCACCGGCGCAGCTCAAATGGACGGTGCAAT
ACTCGTTGTTTCCGCAGCAGACGGCCCCATGCCCCAAACAAGAGAACACGTGCTTCTCGCAAGACAGGTTAACGTTCCTT
ACATAGTCGTTTTCATGAACAAGTGTGATATGGTTGACGACGAAGAACTTTTAGAACTCGTTGAGCTCGAAGTTAGAGAA
CTTCTCTCCAAGTACGAGTATCCCGGAGACGAAGTTCCCGTTATCAGAGGATCGGCACTTGGAGCACTTCAAGAACTCGA
ACAAAACTCTCCCGGCAAGTGGGTTGAGTCCATAAAAGAGCTTCTCAACGCGATGGACGAGTACATACCGACACCACAAA
GGGAAGTGGACAAGCCCTTCCTTATGCCAATAGAAGACGTGTTTAGCATATCCGGAAGAGGAACGGTTGTAACCGGAAGA
GTAGAGAGAGGAGTTCTGAGGCCCGGAGACGAAGTAGAGATAGTAGGACTCAGGGAAGAGCCCCTCAAGACAGTTGCAAC
GTCTATAGAGATGTTCAGGAAGGTGCTTGACGAGGCACTGCCCGGAGACAACATAGGAGTGCTGCTGAGGGGAGTAGGAA
AGGACGACGTAGAAAGGGGACAGGTATTGGCGCAACCTGGGAGTGTAAAGGCTCACAAGAGGTTCAGGGCACAGGTATAT
GTACTTAGCAAGGAGGAAGGAGGAAGACACACTCCGTTCTTTGTGAACTACAGACCGCAGTTTTACTTCAGGACGGCTGA
CGTGACGGGAACTGTAGTGAAGCTTCCAGAAGGAGTGGAAATGGTAATGCCCGGGGACAACGTAGAGCTTGAAGTAGAGC
TCATAGCACCCGTGGCGTTGGAAGAAGGGCTCAGGTTTGCGATAAGGGAAGGTGGTAGAACAGTAGGTGCAGGTGTCGTT
ACTAAAATACTGGATTGA

Upstream 100 bases:

>100_bases
ATGAAATTTTCCCACTACGACGAAGTTCCGCAGCAAATTGCGGAAAAGATTATCGGCGAAAGAATGGCCGGTAAGAGCTC
TTAATTAGGAGGTAATAGAT

Downstream 100 bases:

>100_bases
GGGGATAAATCATGGAACAGGAAAAAATAAGGATAAAACTAAGAGCATACGACCACAGGTTGCTGGATCAATCGGTTAAA
CAGATCATAGAAACCGTAAA

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu

Number of amino acids: Translated: 405; Mature: 404

Protein sequence:

>405_residues
MAKEKFERTKEHVNVGTIGHVDHGKSTLTSAITCVLAAGLVEGGKAKCFKYEEIDKAPEEKERGITINITHVEYETAKRH
YAHVDCPGHADYIKNMITGAAQMDGAILVVSAADGPMPQTREHVLLARQVNVPYIVVFMNKCDMVDDEELLELVELEVRE
LLSKYEYPGDEVPVIRGSALGALQELEQNSPGKWVESIKELLNAMDEYIPTPQREVDKPFLMPIEDVFSISGRGTVVTGR
VERGVLRPGDEVEIVGLREEPLKTVATSIEMFRKVLDEALPGDNIGVLLRGVGKDDVERGQVLAQPGSVKAHKRFRAQVY
VLSKEEGGRHTPFFVNYRPQFYFRTADVTGTVVKLPEGVEMVMPGDNVELEVELIAPVALEEGLRFAIREGGRTVGAGVV
TKILD

Sequences:

>Translated_405_residues
MAKEKFERTKEHVNVGTIGHVDHGKSTLTSAITCVLAAGLVEGGKAKCFKYEEIDKAPEEKERGITINITHVEYETAKRH
YAHVDCPGHADYIKNMITGAAQMDGAILVVSAADGPMPQTREHVLLARQVNVPYIVVFMNKCDMVDDEELLELVELEVRE
LLSKYEYPGDEVPVIRGSALGALQELEQNSPGKWVESIKELLNAMDEYIPTPQREVDKPFLMPIEDVFSISGRGTVVTGR
VERGVLRPGDEVEIVGLREEPLKTVATSIEMFRKVLDEALPGDNIGVLLRGVGKDDVERGQVLAQPGSVKAHKRFRAQVY
VLSKEEGGRHTPFFVNYRPQFYFRTADVTGTVVKLPEGVEMVMPGDNVELEVELIAPVALEEGLRFAIREGGRTVGAGVV
TKILD
>Mature_404_residues
AKEKFERTKEHVNVGTIGHVDHGKSTLTSAITCVLAAGLVEGGKAKCFKYEEIDKAPEEKERGITINITHVEYETAKRHY
AHVDCPGHADYIKNMITGAAQMDGAILVVSAADGPMPQTREHVLLARQVNVPYIVVFMNKCDMVDDEELLELVELEVREL
LSKYEYPGDEVPVIRGSALGALQELEQNSPGKWVESIKELLNAMDEYIPTPQREVDKPFLMPIEDVFSISGRGTVVTGRV
ERGVLRPGDEVEIVGLREEPLKTVATSIEMFRKVLDEALPGDNIGVLLRGVGKDDVERGQVLAQPGSVKAHKRFRAQVYV
LSKEEGGRHTPFFVNYRPQFYFRTADVTGTVVKLPEGVEMVMPGDNVELEVELIAPVALEEGLRFAIREGGRTVGAGVVT
KILD

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily

Homologues:

Organism=Homo sapiens, GI34147630, Length=403, Percent_Identity=56.8238213399504, Blast_Score=467, Evalue=1e-132,
Organism=Homo sapiens, GI4503475, Length=451, Percent_Identity=32.5942350332594, Blast_Score=190, Evalue=2e-48,
Organism=Homo sapiens, GI4503471, Length=446, Percent_Identity=32.7354260089686, Blast_Score=189, Evalue=5e-48,
Organism=Homo sapiens, GI223555963, Length=443, Percent_Identity=31.8284424379233, Blast_Score=175, Evalue=7e-44,
Organism=Homo sapiens, GI5729864, Length=443, Percent_Identity=31.8284424379233, Blast_Score=175, Evalue=8e-44,
Organism=Homo sapiens, GI194018522, Length=349, Percent_Identity=29.7994269340974, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI194097354, Length=349, Percent_Identity=29.7994269340974, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI194018520, Length=349, Percent_Identity=29.7994269340974, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI46094014, Length=350, Percent_Identity=29.1428571428571, Blast_Score=128, Evalue=9e-30,
Organism=Homo sapiens, GI54607086, Length=322, Percent_Identity=28.8819875776398, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI82546879, Length=439, Percent_Identity=25.5125284738041, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI4503507, Length=348, Percent_Identity=27.2988505747126, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI157426893, Length=258, Percent_Identity=28.2945736434109, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI45593140, Length=334, Percent_Identity=24.8502994011976, Blast_Score=69, Evalue=9e-12,
Organism=Homo sapiens, GI53729339, Length=280, Percent_Identity=30, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI53729337, Length=280, Percent_Identity=30, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI94966754, Length=134, Percent_Identity=32.089552238806, Blast_Score=66, Evalue=7e-11,
Organism=Escherichia coli, GI1790412, Length=404, Percent_Identity=70.049504950495, Blast_Score=561, Evalue=1e-161,
Organism=Escherichia coli, GI1789737, Length=404, Percent_Identity=70.049504950495, Blast_Score=561, Evalue=1e-161,
Organism=Escherichia coli, GI2367247, Length=397, Percent_Identity=27.7078085642317, Blast_Score=117, Evalue=9e-28,
Organism=Escherichia coli, GI48994988, Length=244, Percent_Identity=30.7377049180328, Blast_Score=95, Evalue=9e-21,
Organism=Escherichia coli, GI1789108, Length=312, Percent_Identity=29.4871794871795, Blast_Score=90, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17556456, Length=408, Percent_Identity=54.656862745098, Blast_Score=409, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI25141371, Length=398, Percent_Identity=45.4773869346734, Blast_Score=340, Evalue=6e-94,
Organism=Caenorhabditis elegans, GI17552884, Length=459, Percent_Identity=33.5511982570806, Blast_Score=198, Evalue=5e-51,
Organism=Caenorhabditis elegans, GI17569207, Length=459, Percent_Identity=33.5511982570806, Blast_Score=198, Evalue=5e-51,
Organism=Caenorhabditis elegans, GI32566303, Length=452, Percent_Identity=31.6371681415929, Blast_Score=170, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI32566629, Length=457, Percent_Identity=27.5711159737418, Blast_Score=137, Evalue=9e-33,
Organism=Caenorhabditis elegans, GI115532067, Length=438, Percent_Identity=27.6255707762557, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI115532065, Length=438, Percent_Identity=27.6255707762557, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17506081, Length=261, Percent_Identity=32.183908045977, Blast_Score=114, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI32566301, Length=157, Percent_Identity=42.0382165605096, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17509919, Length=331, Percent_Identity=26.2839879154079, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17557151, Length=244, Percent_Identity=29.0983606557377, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17565438, Length=434, Percent_Identity=24.8847926267281, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17563834, Length=433, Percent_Identity=24.4803695150115, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17506493, Length=199, Percent_Identity=32.6633165829146, Blast_Score=66, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6324761, Length=404, Percent_Identity=65.0990099009901, Blast_Score=530, Evalue=1e-151,
Organism=Saccharomyces cerevisiae, GI6325337, Length=449, Percent_Identity=31.8485523385301, Blast_Score=179, Evalue=8e-46,
Organism=Saccharomyces cerevisiae, GI6319594, Length=449, Percent_Identity=31.8485523385301, Blast_Score=179, Evalue=8e-46,
Organism=Saccharomyces cerevisiae, GI6322937, Length=351, Percent_Identity=25.6410256410256, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6320377, Length=444, Percent_Identity=25.9009009009009, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6320863, Length=321, Percent_Identity=26.4797507788162, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323320, Length=253, Percent_Identity=27.2727272727273, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI281363316, Length=402, Percent_Identity=58.2089552238806, Blast_Score=463, Evalue=1e-130,
Organism=Drosophila melanogaster, GI17864358, Length=402, Percent_Identity=58.2089552238806, Blast_Score=463, Evalue=1e-130,
Organism=Drosophila melanogaster, GI19921738, Length=397, Percent_Identity=49.1183879093199, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24652838, Length=459, Percent_Identity=32.6797385620915, Blast_Score=184, Evalue=1e-46,
Organism=Drosophila melanogaster, GI17137572, Length=459, Percent_Identity=32.6797385620915, Blast_Score=184, Evalue=1e-46,
Organism=Drosophila melanogaster, GI45553807, Length=459, Percent_Identity=32.4618736383442, Blast_Score=184, Evalue=1e-46,
Organism=Drosophila melanogaster, GI45553816, Length=459, Percent_Identity=32.4618736383442, Blast_Score=184, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24651721, Length=459, Percent_Identity=32.4618736383442, Blast_Score=184, Evalue=1e-46,
Organism=Drosophila melanogaster, GI17864154, Length=459, Percent_Identity=32.4618736383442, Blast_Score=184, Evalue=1e-46,
Organism=Drosophila melanogaster, GI17137380, Length=350, Percent_Identity=31.4285714285714, Blast_Score=138, Evalue=7e-33,
Organism=Drosophila melanogaster, GI45550900, Length=436, Percent_Identity=28.2110091743119, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI19922690, Length=312, Percent_Identity=30.7692307692308, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24647048, Length=360, Percent_Identity=25.2777777777778, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24647046, Length=360, Percent_Identity=25.2777777777778, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI78706572, Length=257, Percent_Identity=26.4591439688716, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24585709, Length=184, Percent_Identity=33.1521739130435, Blast_Score=66, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24585711, Length=184, Percent_Identity=33.1521739130435, Blast_Score=66, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24585713, Length=184, Percent_Identity=33.1521739130435, Blast_Score=66, Evalue=4e-11,
Organism=Drosophila melanogaster, GI28572034, Length=169, Percent_Identity=36.094674556213, Blast_Score=65, Evalue=1e-10,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): EFTU_AQUAE (O66429)

Other databases:

- EMBL:   AE000657
- EMBL:   AE000657
- PIR:   B70300
- PIR:   G70465
- RefSeq:   NP_212987.1
- RefSeq:   NP_214323.1
- ProteinModelPortal:   O66429
- SMR:   O66429
- GeneID:   1192534
- GeneID:   1193652
- GenomeReviews:   AE000657_GR
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_005
- KEGG:   aae:aq_1928
- NMPDR:   fig|224324.1.peg.2
- HOGENOM:   HBG307581
- OMA:   RDKPFLM
- PhylomeDB:   O66429
- BioCyc:   AAEO224324:AQ_005-MONOMER
- BioCyc:   AAEO224324:AQ_1928-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00118_B
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000
- PANTHER:   PTHR23115:SF31
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00485
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3; SSF50465 Elong_init_C; SSF50447 Translat_factor

EC number: 3.6.5.3

Molecular weight: Translated: 44744; Mature: 44613

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEKFERTKEHVNVGTIGHVDHGKSTLTSAITCVLAAGLVEGGKAKCFKYEEIDKAPEE
CCHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHCCCCH
KERGITINITHVEYETAKRHYAHVDCPGHADYIKNMITGAAQMDGAILVVSAADGPMPQT
HHCCEEEEEEEEEEHHHHCCEEEECCCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCC
REHVLLARQVNVPYIVVFMNKCDMVDDEELLELVELEVRELLSKYEYPGDEVPVIRGSAL
HHHEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCHHH
GALQELEQNSPGKWVESIKELLNAMDEYIPTPQREVDKPFLMPIEDVFSISGRGTVVTGR
HHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHCCCCCCEEEECH
VERGVLRPGDEVEIVGLREEPLKTVATSIEMFRKVLDEALPGDNIGVLLRGVGKDDVERG
HHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHCC
QVLAQPGSVKAHKRFRAQVYVLSKEEGGRHTPFFVNYRPQFYFRTADVTGTVVKLPEGVE
EEEECCCCCHHHHEEEEEEEEEEECCCCCCCCEEEEECCEEEEEECCCCEEEEECCCCCE
MVMPGDNVELEVELIAPVALEEGLRFAIREGGRTVGAGVVTKILD
EEECCCCCEEEEEEECCHHHHHHHHHHHHCCCCEEHHHHHHHHCC
>Mature Secondary Structure 
AKEKFERTKEHVNVGTIGHVDHGKSTLTSAITCVLAAGLVEGGKAKCFKYEEIDKAPEE
CHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHCCCCH
KERGITINITHVEYETAKRHYAHVDCPGHADYIKNMITGAAQMDGAILVVSAADGPMPQT
HHCCEEEEEEEEEEHHHHCCEEEECCCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCC
REHVLLARQVNVPYIVVFMNKCDMVDDEELLELVELEVRELLSKYEYPGDEVPVIRGSAL
HHHEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCHHH
GALQELEQNSPGKWVESIKELLNAMDEYIPTPQREVDKPFLMPIEDVFSISGRGTVVTGR
HHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHCCCCCCEEEECH
VERGVLRPGDEVEIVGLREEPLKTVATSIEMFRKVLDEALPGDNIGVLLRGVGKDDVERG
HHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHCC
QVLAQPGSVKAHKRFRAQVYVLSKEEGGRHTPFFVNYRPQFYFRTADVTGTVVKLPEGVE
EEEECCCCCHHHHEEEEEEEEEEECCCCCCCCEEEEECCEEEEEECCCCEEEEECCCCCE
MVMPGDNVELEVELIAPVALEEGLRFAIREGGRTVGAGVVTKILD
EEECCCCCEEEEEEECCHHHHHHHHHHHHCCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320