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The map label for this gene is celM

Identifier: 11499384

GI number: 11499384

Start: 1611371

End: 1612285

Strand: Reverse

Name: celM

Synonym: AF1795

Alternate gene names: 11499384

Gene position: 1612285-1611371 (Counterclockwise)

Preceding gene: 11499385

Following gene: 11499383

Centisome position: 74.01

GC content: 53.77

Gene sequence:

>915_bases
ATGGGAAATCTGATCTGCACAAAGAATGGAGGAGAGCCGGAGATAATGGTTGCAGCGCACATGGACGAGATTGGCTTTGC
CGTGAAGTACATTGATGATAAGGGGTTCATCAGGATTGCTCCGATCGGCGGGTGGTTTTCCCAGATTGCTTTAGCTCAGA
GAGTCGTTTTGTACGGAAAAAAGAAGGTTTACGGTGTTATCGGCTGCAAGCCTCCCCACCTGATGAAGGATGAAGAGAGG
AAGAAGGGAATCGAGATAAAGGACATGTTCGTCGATATCGGGGCTAGCAGCAAGGAGGAAGTGCTTGAGATGGGCATCAA
CGTAGGCACTCCCGTTGCCCTTGACAGAGAGATCGTGGAGCTGGCAAATGGCAGAATAACGGGCAAAGCCTTCGACAACA
GGGTTGGTGTTGCGGTGATGATCGAGGCGGTGAGAAGGGCAAAGGCTGACATCACCATCCACGCGGTCGCGACGGTTCAG
GAGGAGGTCGGTTTGAAGGGGGCTAGAACTTCGGCCTTCGCCATCGAGCCCACAGCCGCAATTGCAGTTGACACCTGCGT
CGCTGCAGACTTTCCCGGCAGCGAGTCGGCGCACATGGACGTCAAGCTCGGTAAGGGAGCGGCAATCACAGTCATAGACG
CATCGGGGAGGGGGTTGATTGCCTCTCCAAAGGTTTTGAGGTGGCTGACCGAGACAGCTGAAAAATACGGCATCCCCTAT
CAGCTTGAGGTTGCTGAGGGCGGAACCACCGATGCAACTGCAATTCACCTCACAAAGGCCGGAATTCCAGCAGGGGTTGT
CAGCGTGCCTGCAAGATACATTCACAGCCCTGTGGAGGTTGTTGACTTGAAGGACGTTGAGAGCGCTGTAGAGATTGTGG
CAAGAGCAATCGAGAACGCCAAAAACTACTTCTGA

Upstream 100 bases:

>100_bases
ACTGAGCAACGCTCACGGAATTAGCGGTTATGAGGATGAAGTCAGAGAGATCGTAAGGGCGGAGCTTGAAGACTGGGCTG
ATGAAGTAAAGACGGACAGC

Downstream 100 bases:

>100_bases
AGGAAATTTTTTATTCCGAGAGGTTCGAGAACACACAGACATCTCCAATTTCTCTGAGGAGGTGATGCTTAATGAAGGTC
TGGCTGGTAGGCGCTTATGG

Product: endoglucanase (celM)

Products: D-glucose; GDP

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MGNLICTKNGGEPEIMVAAHMDEIGFAVKYIDDKGFIRIAPIGGWFSQIALAQRVVLYGKKKVYGVIGCKPPHLMKDEER
KKGIEIKDMFVDIGASSKEEVLEMGINVGTPVALDREIVELANGRITGKAFDNRVGVAVMIEAVRRAKADITIHAVATVQ
EEVGLKGARTSAFAIEPTAAIAVDTCVAADFPGSESAHMDVKLGKGAAITVIDASGRGLIASPKVLRWLTETAEKYGIPY
QLEVAEGGTTDATAIHLTKAGIPAGVVSVPARYIHSPVEVVDLKDVESAVEIVARAIENAKNYF

Sequences:

>Translated_304_residues
MGNLICTKNGGEPEIMVAAHMDEIGFAVKYIDDKGFIRIAPIGGWFSQIALAQRVVLYGKKKVYGVIGCKPPHLMKDEER
KKGIEIKDMFVDIGASSKEEVLEMGINVGTPVALDREIVELANGRITGKAFDNRVGVAVMIEAVRRAKADITIHAVATVQ
EEVGLKGARTSAFAIEPTAAIAVDTCVAADFPGSESAHMDVKLGKGAAITVIDASGRGLIASPKVLRWLTETAEKYGIPY
QLEVAEGGTTDATAIHLTKAGIPAGVVSVPARYIHSPVEVVDLKDVESAVEIVARAIENAKNYF
>Mature_303_residues
GNLICTKNGGEPEIMVAAHMDEIGFAVKYIDDKGFIRIAPIGGWFSQIALAQRVVLYGKKKVYGVIGCKPPHLMKDEERK
KGIEIKDMFVDIGASSKEEVLEMGINVGTPVALDREIVELANGRITGKAFDNRVGVAVMIEAVRRAKADITIHAVATVQE
EVGLKGARTSAFAIEPTAAIAVDTCVAADFPGSESAHMDVKLGKGAAITVIDASGRGLIASPKVLRWLTETAEKYGIPYQ
LEVAEGGTTDATAIHLTKAGIPAGVVSVPARYIHSPVEVVDLKDVESAVEIVARAIENAKNYF

Specific function: Unknown

COG id: COG1363

COG function: function code G; Cellulase M and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M42 family [H]

Homologues:

Organism=Escherichia coli, GI1790332, Length=296, Percent_Identity=33.445945945946, Blast_Score=195, Evalue=3e-51,
Organism=Escherichia coli, GI87082417, Length=305, Percent_Identity=35.4098360655738, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI1788727, Length=291, Percent_Identity=32.6460481099656, Blast_Score=157, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008007 [H]

Pfam domain/function: PF05343 Peptidase_M42 [H]

EC number: 3.2.1.4

Molecular weight: Translated: 32382; Mature: 32251

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNLICTKNGGEPEIMVAAHMDEIGFAVKYIDDKGFIRIAPIGGWFSQIALAQRVVLYGK
CCCEEEECCCCCCEEEEEEECCCCCEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHCCC
KKVYGVIGCKPPHLMKDEERKKGIEIKDMFVDIGASSKEEVLEMGINVGTPVALDREIVE
CEEEEEECCCCCCCCCCHHHHCCCEEEEEEEECCCCCHHHHHHHCCCCCCCCHHHHHHHH
LANGRITGKAFDNRVGVAVMIEAVRRAKADITIHAVATVQEEVGLKGARTSAFAIEPTAA
HHCCEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEEEEEHHHHCCCCCCCCEEEECCCHH
IAVDTCVAADFPGSESAHMDVKLGKGAAITVIDASGRGLIASPKVLRWLTETAEKYGIPY
EEEHHHEECCCCCCCCCEEEEEECCCEEEEEEECCCCCEECCHHHHHHHHHHHHHHCCCE
QLEVAEGGTTDATAIHLTKAGIPAGVVSVPARYIHSPVEVVDLKDVESAVEIVARAIENA
EEEECCCCCCCCEEEEEEECCCCCCHHHHCHHHHCCCCEEEEHHHHHHHHHHHHHHHHHH
KNYF
HCCC
>Mature Secondary Structure 
GNLICTKNGGEPEIMVAAHMDEIGFAVKYIDDKGFIRIAPIGGWFSQIALAQRVVLYGK
CCEEEECCCCCCEEEEEEECCCCCEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHCCC
KKVYGVIGCKPPHLMKDEERKKGIEIKDMFVDIGASSKEEVLEMGINVGTPVALDREIVE
CEEEEEECCCCCCCCCCHHHHCCCEEEEEEEECCCCCHHHHHHHCCCCCCCCHHHHHHHH
LANGRITGKAFDNRVGVAVMIEAVRRAKADITIHAVATVQEEVGLKGARTSAFAIEPTAA
HHCCEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEEEEEHHHHCCCCCCCCEEEECCCHH
IAVDTCVAADFPGSESAHMDVKLGKGAAITVIDASGRGLIASPKVLRWLTETAEKYGIPY
EEEHHHEECCCCCCCCCEEEEEECCCEEEEEEECCCCCEECCHHHHHHHHHHHHHHCCCE
QLEVAEGGTTDATAIHLTKAGIPAGVVSVPARYIHSPVEVVDLKDVESAVEIVARAIENA
EEEECCCCCCCCEEEEEEECCCCCCHHHHCHHHHCCCCEEEEHHHHHHHHHHHHHHHHHH
KNYF
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDPglucose; H2O

Specific reaction: GDP-glucose + H2O = D-glucose + GDP

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]