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The map label for this gene is ilvB-1

Identifier: 11499309

GI number: 11499309

Start: 1539831

End: 1541489

Strand: Reverse

Name: ilvB-1

Synonym: AF1720

Alternate gene names: 11499309

Gene position: 1541489-1539831 (Counterclockwise)

Preceding gene: 11499314

Following gene: 11499308

Centisome position: 70.76

GC content: 53.83

Gene sequence:

>1659_bases
ATGCGTGCTGCTGATGCCATTGTCAAGGCTTTGGAGATGGAAGGTATAGAGGTCGTGTTTGGAATACCCGGAGGTGCGAT
TATTGAGGTCTACGATGCGCTCTACGATTCGGGAATAAGGCACATAACAACGAGGCACGAGCAGGGTGCGACCCACGCGG
CAGACGGCTACGCAAGGGCGAGCGGCAAAGTGGGGGTTGCCTTCGCCACCTCCGGACCCGGAGCGACGAACACCGTTACC
GGCATAGCTACAGCCTACATGGACTCCTCACCGCTCGTTGTCTTCACCGGCCAGGTTCCGACCAGCATGATCGGAAACGA
TGCGTTTCAGGAGGCCGACATAACCGGAATCACGATGCCCATAACCAAGCACAACTACCTGGTTACGGACACCAAAGACC
TTCTGGGCACGATAAAGGAGGCTTTCCACATAGCCTCAACCGGCAGACCCGGTCCGGTTCTCGTTGACCTGCCCAAGGAC
ATCACAACCGCAGACATAGACTTCAACTACCCCAAGAAGGTCAGCCTCCCGGGATACAAGCCGAAGCTGGAGGGGCATCC
GAGGCAGATAGCGAAGGCGGCCGAGCTGATAATGAAGGCGGAGAGACCGGTTATACTTGCCGGTGGTGGGGTCATAATCT
CAAACGCCTCAAAGGAGCTTGTCGAGCTTGCAGAAACAATCCCGGCCTTCGTTGTTACAACGCTGATGGGAAAAGGCTCA
ATTCCAGAAACACACCCGCTGTCACTCGGATTCGCCGGAATGCACGGAACGAAGTACGCAAACTACGCTTTGAGTGAGAG
CGACTTGATTATCGCGGTCGGCTGCAGGTTCAGCGACAGAACAACCGGAAACGTCGCGATGTTCGCACCAGAGGCAAAGA
TAATCCACATCGACATCGATCCTGCAGAAATAGGAAAAAACGTCAGAGTTGACGTCCCTATAGTCGGAGATGCGAGGAAA
GTCCTTGCAAAGCTTAAGAAGGCCGTGGAGTACAAGCAAAGAAAAGAATGGGAGGACAAGGTGAACGACTGGAAGAAGAG
ATATCCGCTGAAGTACAAAAAGGAGGGGTTCAAGCCGCAGTACGTGATTGAGCGCGCCTGCGAGATTATGCCGGACGCTA
TTATCACGACAGAGGTTGGACAGAATCAGATGTGGGCTGCTCAGTTTTTCAAAACCAAGTACCCAAGGCAGTTCATAACA
AGCGGTGGATTGGGAACGATGGGTTTCGGCTTCCCTGCAGCGATGGGCGCACAGGTCGCGTTTCCAGAAAAGACAGTTAT
AGACATCGCAGGGGATGGGAGCTTCTTCATGAACATCCAGGAGCTTGCAACATGCGTCAAGTACGAGATTCCCGTGAAAG
TTCTGGTTCTGAATAACGGCTACCTCGGAATGGTCAGGCAGTGGCAGGAGCTGTTCTACAGAGAGAGGTACTCTGCAACC
TGCATTGGCTGCGAGAAAACGGGATTTGAAGCCATAGCAAGGGGTTTCGGTGCGATAGGCATGACCGTCGAGAAGCCTTC
GGAAGTCGATGATGCACTCAAGGAGGCAAAGGAAGTCGACGCGCCGGTGGTCATAGACTTCAGAGTTGACTACCAGGCAA
ACGTCTTCCCGATGGTCCCGCCGGGAGCGGCGTTAAACGAGATTATCGATGAGGAGTGA

Upstream 100 bases:

>100_bases
GCGCATGCTGGTGGTGGGAGTGATGAAAAAGTTCTCTGGAAAAGAGTTCATTGTAGTACAGCTTTTACTTCTTTTCTAAA
GGAGTTAGAGGTGGTTTTAA

Downstream 100 bases:

>100_bases
GGTGGTAGCGTGAAGCACACGATAGCCGTTCTGGTTGAGAACAAGCCCGGAGTCCTTGCGAGGGTTGCGGGACTGTTCAG
GAGGAGGGGTTTCAACATAG

Product: acetolactate synthase 3 catalytic subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 552; Mature: 552

Protein sequence:

>552_residues
MRAADAIVKALEMEGIEVVFGIPGGAIIEVYDALYDSGIRHITTRHEQGATHAADGYARASGKVGVAFATSGPGATNTVT
GIATAYMDSSPLVVFTGQVPTSMIGNDAFQEADITGITMPITKHNYLVTDTKDLLGTIKEAFHIASTGRPGPVLVDLPKD
ITTADIDFNYPKKVSLPGYKPKLEGHPRQIAKAAELIMKAERPVILAGGGVIISNASKELVELAETIPAFVVTTLMGKGS
IPETHPLSLGFAGMHGTKYANYALSESDLIIAVGCRFSDRTTGNVAMFAPEAKIIHIDIDPAEIGKNVRVDVPIVGDARK
VLAKLKKAVEYKQRKEWEDKVNDWKKRYPLKYKKEGFKPQYVIERACEIMPDAIITTEVGQNQMWAAQFFKTKYPRQFIT
SGGLGTMGFGFPAAMGAQVAFPEKTVIDIAGDGSFFMNIQELATCVKYEIPVKVLVLNNGYLGMVRQWQELFYRERYSAT
CIGCEKTGFEAIARGFGAIGMTVEKPSEVDDALKEAKEVDAPVVIDFRVDYQANVFPMVPPGAALNEIIDEE

Sequences:

>Translated_552_residues
MRAADAIVKALEMEGIEVVFGIPGGAIIEVYDALYDSGIRHITTRHEQGATHAADGYARASGKVGVAFATSGPGATNTVT
GIATAYMDSSPLVVFTGQVPTSMIGNDAFQEADITGITMPITKHNYLVTDTKDLLGTIKEAFHIASTGRPGPVLVDLPKD
ITTADIDFNYPKKVSLPGYKPKLEGHPRQIAKAAELIMKAERPVILAGGGVIISNASKELVELAETIPAFVVTTLMGKGS
IPETHPLSLGFAGMHGTKYANYALSESDLIIAVGCRFSDRTTGNVAMFAPEAKIIHIDIDPAEIGKNVRVDVPIVGDARK
VLAKLKKAVEYKQRKEWEDKVNDWKKRYPLKYKKEGFKPQYVIERACEIMPDAIITTEVGQNQMWAAQFFKTKYPRQFIT
SGGLGTMGFGFPAAMGAQVAFPEKTVIDIAGDGSFFMNIQELATCVKYEIPVKVLVLNNGYLGMVRQWQELFYRERYSAT
CIGCEKTGFEAIARGFGAIGMTVEKPSEVDDALKEAKEVDAPVVIDFRVDYQANVFPMVPPGAALNEIIDEE
>Mature_552_residues
MRAADAIVKALEMEGIEVVFGIPGGAIIEVYDALYDSGIRHITTRHEQGATHAADGYARASGKVGVAFATSGPGATNTVT
GIATAYMDSSPLVVFTGQVPTSMIGNDAFQEADITGITMPITKHNYLVTDTKDLLGTIKEAFHIASTGRPGPVLVDLPKD
ITTADIDFNYPKKVSLPGYKPKLEGHPRQIAKAAELIMKAERPVILAGGGVIISNASKELVELAETIPAFVVTTLMGKGS
IPETHPLSLGFAGMHGTKYANYALSESDLIIAVGCRFSDRTTGNVAMFAPEAKIIHIDIDPAEIGKNVRVDVPIVGDARK
VLAKLKKAVEYKQRKEWEDKVNDWKKRYPLKYKKEGFKPQYVIERACEIMPDAIITTEVGQNQMWAAQFFKTKYPRQFIT
SGGLGTMGFGFPAAMGAQVAFPEKTVIDIAGDGSFFMNIQELATCVKYEIPVKVLVLNNGYLGMVRQWQELFYRERYSAT
CIGCEKTGFEAIARGFGAIGMTVEKPSEVDDALKEAKEVDAPVVIDFRVDYQANVFPMVPPGAALNEIIDEE

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=561, Percent_Identity=26.0249554367201, Blast_Score=194, Evalue=2e-49,
Organism=Homo sapiens, GI21361361, Length=590, Percent_Identity=27.1186440677966, Blast_Score=183, Evalue=4e-46,
Organism=Escherichia coli, GI87081685, Length=564, Percent_Identity=46.2765957446808, Blast_Score=525, Evalue=1e-150,
Organism=Escherichia coli, GI1790104, Length=553, Percent_Identity=47.377938517179, Blast_Score=472, Evalue=1e-134,
Organism=Escherichia coli, GI1786717, Length=570, Percent_Identity=37.3684210526316, Blast_Score=350, Evalue=2e-97,
Organism=Escherichia coli, GI1787096, Length=542, Percent_Identity=30.2583025830258, Blast_Score=226, Evalue=4e-60,
Organism=Escherichia coli, GI1788716, Length=558, Percent_Identity=26.7025089605735, Blast_Score=169, Evalue=6e-43,
Organism=Caenorhabditis elegans, GI17531301, Length=557, Percent_Identity=27.8276481149013, Blast_Score=175, Evalue=6e-44,
Organism=Caenorhabditis elegans, GI17531299, Length=557, Percent_Identity=27.8276481149013, Blast_Score=175, Evalue=7e-44,
Organism=Caenorhabditis elegans, GI17542570, Length=564, Percent_Identity=26.241134751773, Blast_Score=171, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6323755, Length=567, Percent_Identity=46.7372134038801, Blast_Score=470, Evalue=1e-133,
Organism=Saccharomyces cerevisiae, GI6320816, Length=541, Percent_Identity=26.4325323475046, Blast_Score=136, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6320123, Length=529, Percent_Identity=23.8185255198488, Blast_Score=111, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6323163, Length=486, Percent_Identity=25.1028806584362, Blast_Score=104, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6321524, Length=492, Percent_Identity=26.4227642276423, Blast_Score=103, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6323073, Length=502, Percent_Identity=25.8964143426295, Blast_Score=100, Evalue=4e-22,
Organism=Drosophila melanogaster, GI19922626, Length=562, Percent_Identity=26.3345195729537, Blast_Score=172, Evalue=7e-43,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 59975; Mature: 59975

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAADAIVKALEMEGIEVVFGIPGGAIIEVYDALYDSGIRHITTRHEQGATHAADGYARA
CCHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHCCCHHEECCCCCCCCCCCCCHHCC
SGKVGVAFATSGPGATNTVTGIATAYMDSSPLVVFTGQVPTSMIGNDAFQEADITGITMP
CCCEEEEEEECCCCCCCHHHHHEEEEECCCCEEEEECCCCHHHHCCCHHCCCCCCEEEEE
ITKHNYLVTDTKDLLGTIKEAFHIASTGRPGPVLVDLPKDITTADIDFNYPKKVSLPGYK
EECCCEEEECHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEEEEECCCCCCCCCCCCCC
PKLEGHPRQIAKAAELIMKAERPVILAGGGVIISNASKELVELAETIPAFVVTTLMGKGS
CCCCCCHHHHHHHHHHHHHCCCCEEEECCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCC
IPETHPLSLGFAGMHGTKYANYALSESDLIIAVGCRFSDRTTGNVAMFAPEAKIIHIDID
CCCCCCCEEEEECCCCCCCCCEEECCCCEEEEEECCCCCCCCCCEEEEECCCEEEEEECC
PAEIGKNVRVDVPIVGDARKVLAKLKKAVEYKQRKEWEDKVNDWKKRYPLKYKKEGFKPQ
HHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCHH
YVIERACEIMPDAIITTEVGQNQMWAAQFFKTKYPRQFITSGGLGTMGFGFPAAMGAQVA
HHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCHHHHHCCCCCCCCCCCHHHCCCEEE
FPEKTVIDIAGDGSFFMNIQELATCVKYEIPVKVLVLNNGYLGMVRQWQELFYRERYSAT
CCCCEEEEEECCCCEEEEHHHHHHHHEEECCEEEEEECCCCHHHHHHHHHHHHHHCCCEE
CIGCEKTGFEAIARGFGAIGMTVEKPSEVDDALKEAKEVDAPVVIDFRVDYQANVFPMVP
EEECCCCCHHHHHHCCCCCCEEECCCHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEECC
PGAALNEIIDEE
CCHHHHHHHCCC
>Mature Secondary Structure
MRAADAIVKALEMEGIEVVFGIPGGAIIEVYDALYDSGIRHITTRHEQGATHAADGYARA
CCHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHCCCHHEECCCCCCCCCCCCCHHCC
SGKVGVAFATSGPGATNTVTGIATAYMDSSPLVVFTGQVPTSMIGNDAFQEADITGITMP
CCCEEEEEEECCCCCCCHHHHHEEEEECCCCEEEEECCCCHHHHCCCHHCCCCCCEEEEE
ITKHNYLVTDTKDLLGTIKEAFHIASTGRPGPVLVDLPKDITTADIDFNYPKKVSLPGYK
EECCCEEEECHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEEEEECCCCCCCCCCCCCC
PKLEGHPRQIAKAAELIMKAERPVILAGGGVIISNASKELVELAETIPAFVVTTLMGKGS
CCCCCCHHHHHHHHHHHHHCCCCEEEECCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCC
IPETHPLSLGFAGMHGTKYANYALSESDLIIAVGCRFSDRTTGNVAMFAPEAKIIHIDID
CCCCCCCEEEEECCCCCCCCCEEECCCCEEEEEECCCCCCCCCCEEEEECCCEEEEEECC
PAEIGKNVRVDVPIVGDARKVLAKLKKAVEYKQRKEWEDKVNDWKKRYPLKYKKEGFKPQ
HHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCHH
YVIERACEIMPDAIITTEVGQNQMWAAQFFKTKYPRQFITSGGLGTMGFGFPAAMGAQVA
HHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCHHHHHCCCCCCCCCCCHHHCCCEEE
FPEKTVIDIAGDGSFFMNIQELATCVKYEIPVKVLVLNNGYLGMVRQWQELFYRERYSAT
CCCCEEEEEECCCCEEEEHHHHHHHHEEECCEEEEEECCCCHHHHHHHHHHHHHHCCCEE
CIGCEKTGFEAIARGFGAIGMTVEKPSEVDDALKEAKEVDAPVVIDFRVDYQANVFPMVP
EEECCCCCHHHHHHCCCCCCEEECCCHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEECC
PGAALNEIIDEE
CCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]