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The map label for this gene is pcbD

Identifier: 11499296

GI number: 11499296

Start: 1527768

End: 1528484

Strand: Reverse

Name: pcbD

Synonym: AF1706

Alternate gene names: 11499296

Gene position: 1528484-1527768 (Counterclockwise)

Preceding gene: 11499297

Following gene: 11499294

Centisome position: 70.17

GC content: 48.4

Gene sequence:

>717_bases
GTGAAGAAAATTCAGGCTATGAGATTCGGCTTTTTCGACTCGGATGGTTTGAAGCTTCGCTACTTCGAGTGCGGCGAGGG
GGAGCCCTTAATCTTAATCCACGGACTTGGGGACTGCATAGAGGGGTGGACTTTTCAGTATGACGACTTCTCCAAGCACT
TCAGAGTTGTTGCGCTCGATTTGAGAGGATTCGGAATGTCAGACGTGCCTGAAAGCATTTCCGTAGAGGACTTCGCCAGA
GACGTGAAAAACCTCATAGACCACCTTGGAATTGAGAGGGCAAACCTCCTCGGCCTCTCAATGGGTGGAGTTGTCTGCAT
GGAGTTCTACAGGCAGTATCCGGAGATGGTGAAGTCTCTCGTTCTGGCAAACACCCTCCACAAGCTCCCAGATGCAGGAA
GGGCTATGTTTGAGCAGAGATTGAAGCTGCTGGAAAGCTCACCCGACATGACCCAAATTGCGGAATTCATAGCGGAAATG
TCCATACACCAAGACAGGCCTGAGCTGAAAGAGCTGGTGAAAACTATAATCAGAAAGAACAACAAGGAGTTTTACACAAA
GGTGACAGCCGAACTGGGAAGGATAAACTACGAAGACTTGCTTCCCAAAATAGAAGTCCCAACTCTGGTAATCACAGCTG
AGTTTGATGTAACAACACCACCCGCCTTAGGTAGGGAAATTGCCTCGCTGATTCCCAAATGTGATATCCCGAATTAA

Upstream 100 bases:

>100_bases
TTAGGACTTGATATTCTGTTTTTTTGCTTAAATAGACATTTCAGCTGTCTCCAGAGGTTACAGGCATAGATAAACAAAAA
AGTAATTGGCTTTTTAAATA

Downstream 100 bases:

>100_bases
TTTCACTCCTCAAACAACCACCTCCCGTACTCAAACACCCTTTCAGCAGGTAGCTTCCTCAAAAAGGCCTGATAGGGAGT
CTCCAATTCGTCGAAATTCA

Product: 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid hydrolase (pcbD)

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MKKIQAMRFGFFDSDGLKLRYFECGEGEPLILIHGLGDCIEGWTFQYDDFSKHFRVVALDLRGFGMSDVPESISVEDFAR
DVKNLIDHLGIERANLLGLSMGGVVCMEFYRQYPEMVKSLVLANTLHKLPDAGRAMFEQRLKLLESSPDMTQIAEFIAEM
SIHQDRPELKELVKTIIRKNNKEFYTKVTAELGRINYEDLLPKIEVPTLVITAEFDVTTPPALGREIASLIPKCDIPN

Sequences:

>Translated_238_residues
MKKIQAMRFGFFDSDGLKLRYFECGEGEPLILIHGLGDCIEGWTFQYDDFSKHFRVVALDLRGFGMSDVPESISVEDFAR
DVKNLIDHLGIERANLLGLSMGGVVCMEFYRQYPEMVKSLVLANTLHKLPDAGRAMFEQRLKLLESSPDMTQIAEFIAEM
SIHQDRPELKELVKTIIRKNNKEFYTKVTAELGRINYEDLLPKIEVPTLVITAEFDVTTPPALGREIASLIPKCDIPN
>Mature_238_residues
MKKIQAMRFGFFDSDGLKLRYFECGEGEPLILIHGLGDCIEGWTFQYDDFSKHFRVVALDLRGFGMSDVPESISVEDFAR
DVKNLIDHLGIERANLLGLSMGGVVCMEFYRQYPEMVKSLVLANTLHKLPDAGRAMFEQRLKLLESSPDMTQIAEFIAEM
SIHQDRPELKELVKTIIRKNNKEFYTKVTAELGRINYEDLLPKIEVPTLVITAEFDVTTPPALGREIASLIPKCDIPN

Specific function: 3-hydroxyphenylpropionate degradation. [C]

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dmpD/todF/xylF esterase family [H]

Homologues:

Organism=Homo sapiens, GI218777837, Length=118, Percent_Identity=36.4406779661017, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI218777840, Length=122, Percent_Identity=33.6065573770492, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI13376164, Length=122, Percent_Identity=33.6065573770492, Blast_Score=72, Evalue=4e-13,
Organism=Escherichia coli, GI87081721, Length=245, Percent_Identity=28.1632653061224, Blast_Score=71, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17554012, Length=179, Percent_Identity=31.2849162011173, Blast_Score=84, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17562298, Length=122, Percent_Identity=27.0491803278689, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24586385, Length=101, Percent_Identity=36.6336633663366, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24586387, Length=101, Percent_Identity=36.6336633663366, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24586391, Length=101, Percent_Identity=36.6336633663366, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24586389, Length=101, Percent_Identity=36.6336633663366, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 27048; Mature: 27048

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIQAMRFGFFDSDGLKLRYFECGEGEPLILIHGLGDCIEGWTFQYDDFSKHFRVVALD
CCCHHHHHCCCCCCCCCEEEEEECCCCCCEEEEECCHHHHCCCCCCCCCCCCCEEEEEEE
LRGFGMSDVPESISVEDFARDVKNLIDHLGIERANLLGLSMGGVVCMEFYRQYPEMVKSL
ECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHH
VLANTLHKLPDAGRAMFEQRLKLLESSPDMTQIAEFIAEMSIHQDRPELKELVKTIIRKN
HHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCC
NKEFYTKVTAELGRINYEDLLPKIEVPTLVITAEFDVTTPPALGREIASLIPKCDIPN
CHHHHHHHHHHHHCCCHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKKIQAMRFGFFDSDGLKLRYFECGEGEPLILIHGLGDCIEGWTFQYDDFSKHFRVVALD
CCCHHHHHCCCCCCCCCEEEEEECCCCCCEEEEECCHHHHCCCCCCCCCCCCCEEEEEEE
LRGFGMSDVPESISVEDFARDVKNLIDHLGIERANLLGLSMGGVVCMEFYRQYPEMVKSL
ECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHH
VLANTLHKLPDAGRAMFEQRLKLLESSPDMTQIAEFIAEMSIHQDRPELKELVKTIIRKN
HHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCC
NKEFYTKVTAELGRINYEDLLPKIEVPTLVITAEFDVTTPPALGREIASLIPKCDIPN
CHHHHHHHHHHHHCCCHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]