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The map label for this gene is est-2

Identifier: 11499132

GI number: 11499132

Start: 1385351

End: 1386106

Strand: Reverse

Name: est-2

Synonym: AF1537

Alternate gene names: 11499132

Gene position: 1386106-1385351 (Counterclockwise)

Preceding gene: 11499133

Following gene: 11499131

Centisome position: 63.63

GC content: 46.69

Gene sequence:

>756_bases
ATGCTGGAGAGAGTATTCATCGACGTGGATGGAGTAAAGGTCTCTCTCCTCAAAGGGAGGGAGAGGAAAGTTTTTTACAT
ACACAGCTCCGGGAGCGACGCCACTCAGTGGGTAAATCAGTTGACGGCAATCGGGGGTTACGCAATAGACCTCCCCAATC
ACGGCCAGAGCGACACCGTGGAGGTTAATTCGGTTGACGAATACGCTTATTATGCCAGTGAGAGCTTGAAGAAAACCGTT
GGGAAAGCCGTCGTAGTGGGTCATAGCTTAGGTGGGGCTGTAGCTCAAAAATTATATCTTAGAAATCCTGAAATCTGCTT
GGCTCTCGTTCTGGTAGGGACTGGAGCGAGGCTGAGGGTTCTTCCTGAGATCTTGGAAGGCCTAAAAAAGGAACCTGAAA
AGGCGGTTGACTTAATGTTAAGTATGGCGTTTGCCAGCAAAGGAGAGGAATACGAGAAGAAAAGACGAGAGTTCTTGGAT
AGAGTTGATGTGCTACACCTCGATCTCAGTTTGTGTGATAGATTTGATTTGCTTGAAGACTACAGGAACGGGAAGTTGAA
AATAGGTGTCCCAACTCTCGTAATTGTCGGCGAGGAGGACAAGCTAACGCCGCTGAAGTATCACGAATTTTTTCACAAGC
ACATTCCAAATTCAGAACTAGTCGTAATACCTGGCGCCAGCCACATGGTAATGCTTGAAAAGCACGTGGAATTCAACGAG
GCGCTTGAAAAGTTTCTCAAAAAAGTTGGGGTTTAA

Upstream 100 bases:

>100_bases
CACGATCTCCGTGATGATATTGGCAACCATTCCGCTCTCGCTCGGTTTAGTTCTCGTTCCATATTGATCTAATTTTTTTT
ATATTGAGGAGGGAAAAGCT

Downstream 100 bases:

>100_bases
TTTAGACTAAGCTTTTCCCCACAACTTAACCACAGCAAAATAGCGAAGAGCCTAAACAGCGTCTTTGAATTAAAATCAAA
TTCACCAAAGTTATTAAAAA

Product: carboxylesterase (est-2)

Products: alcohol; carboxylate

Alternate protein names: Alpha/Beta Hydrolase Fold; Hydrolase; Carboxylesterase; Alpha/Beta Hydrolase; 3-Oxoadipate Enol-Lactonase; Alpha/Beta Hydrolase Fold-Containing Protein; Hydrolase Or Acyltransferase Protein; Biotin Biosynthesis Protein BioH; Hydrolase Alpha/Beta Fold Family; Hydrolase Alpha/Beta Fold Family Protein; Hydrolase Or Acyltransferase Of Alpha/Beta Superfamily

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MLERVFIDVDGVKVSLLKGRERKVFYIHSSGSDATQWVNQLTAIGGYAIDLPNHGQSDTVEVNSVDEYAYYASESLKKTV
GKAVVVGHSLGGAVAQKLYLRNPEICLALVLVGTGARLRVLPEILEGLKKEPEKAVDLMLSMAFASKGEEYEKKRREFLD
RVDVLHLDLSLCDRFDLLEDYRNGKLKIGVPTLVIVGEEDKLTPLKYHEFFHKHIPNSELVVIPGASHMVMLEKHVEFNE
ALEKFLKKVGV

Sequences:

>Translated_251_residues
MLERVFIDVDGVKVSLLKGRERKVFYIHSSGSDATQWVNQLTAIGGYAIDLPNHGQSDTVEVNSVDEYAYYASESLKKTV
GKAVVVGHSLGGAVAQKLYLRNPEICLALVLVGTGARLRVLPEILEGLKKEPEKAVDLMLSMAFASKGEEYEKKRREFLD
RVDVLHLDLSLCDRFDLLEDYRNGKLKIGVPTLVIVGEEDKLTPLKYHEFFHKHIPNSELVVIPGASHMVMLEKHVEFNE
ALEKFLKKVGV
>Mature_251_residues
MLERVFIDVDGVKVSLLKGRERKVFYIHSSGSDATQWVNQLTAIGGYAIDLPNHGQSDTVEVNSVDEYAYYASESLKKTV
GKAVVVGHSLGGAVAQKLYLRNPEICLALVLVGTGARLRVLPEILEGLKKEPEKAVDLMLSMAFASKGEEYEKKRREFLD
RVDVLHLDLSLCDRFDLLEDYRNGKLKIGVPTLVIVGEEDKLTPLKYHEFFHKHIPNSELVVIPGASHMVMLEKHVEFNE
ALEKFLKKVGV

Specific function: Not Clear. Seems To Be Implicated In The Early Steps Of Biotin Biosynthesis. [C]

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.1.1.1

Molecular weight: Translated: 28119; Mature: 28119

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLERVFIDVDGVKVSLLKGRERKVFYIHSSGSDATQWVNQLTAIGGYAIDLPNHGQSDTV
CCCEEEEEECCEEEEEECCCCEEEEEEECCCCHHHHHHHHHHHHCCEEEECCCCCCCCEE
EVNSVDEYAYYASESLKKTVGKAVVVGHSLGGAVAQKLYLRNPEICLALVLVGTGARLRV
EECCCHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHCCCCHHEEEEEECCCCCHHHH
LPEILEGLKKEPEKAVDLMLSMAFASKGEEYEKKRREFLDRVDVLHLDLSLCDRFDLLED
HHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YRNGKLKIGVPTLVIVGEEDKLTPLKYHEFFHKHIPNSELVVIPGASHMVMLEKHVEFNE
HCCCEEEECCCEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCEEEEHHHCCHHH
ALEKFLKKVGV
HHHHHHHHHCC
>Mature Secondary Structure
MLERVFIDVDGVKVSLLKGRERKVFYIHSSGSDATQWVNQLTAIGGYAIDLPNHGQSDTV
CCCEEEEEECCEEEEEECCCCEEEEEEECCCCHHHHHHHHHHHHCCEEEECCCCCCCCEE
EVNSVDEYAYYASESLKKTVGKAVVVGHSLGGAVAQKLYLRNPEICLALVLVGTGARLRV
EECCCHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHCCCCHHEEEEEECCCCCHHHH
LPEILEGLKKEPEKAVDLMLSMAFASKGEEYEKKRREFLDRVDVLHLDLSLCDRFDLLED
HHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YRNGKLKIGVPTLVIVGEEDKLTPLKYHEFFHKHIPNSELVVIPGASHMVMLEKHVEFNE
HCCCEEEECCCEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCEEEEHHHCCHHH
ALEKFLKKVGV
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: carboxylic ester; H2O

Specific reaction: A carboxylic ester + H2O = an alcohol + a carboxylate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA