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The map label for this gene is gatB-1

Identifier: 11499035

GI number: 11499035

Start: 1297277

End: 1299118

Strand: Reverse

Name: gatB-1

Synonym: AF1440

Alternate gene names: 11499035

Gene position: 1299118-1297277 (Counterclockwise)

Preceding gene: 11499036

Following gene: 11499034

Centisome position: 59.64

GC content: 51.95

Gene sequence:

>1842_bases
ATGATGGATTACAAGAAGCTGGGACTTAAGGTTGGAATTGAGATTCACCAGCAGCTCGACACAAAGCACAAGCTCTTCTG
CCTCTGCCCTACCCTAACGAGGGAGGTTGAGGAGTCTAACTTTGAATTTTTCAGGTATTTGAGGCTGAAGAGGAGCGAAA
TTGGTGAAGAGGACAGGGCGGCAAAGGAGGAGGTTGAGAGGAGCAGGAGGTTTATTTACAAGTACTATGACACCACATGC
CTTGTCGAGGCCGACGAGGAGCCGCCGAGGGAGGTAAACAGAGAGGCTCTGCTTATAGCCATTCAGGTTGCGAAAATGCT
GAACATGGAGGTAGTTGATGAAGTGCATGTGATGAGAAAAATTGTCATCGACGGGAGCAACACAACGGGCTTTCAGAGGA
CGGCTCTGGTGGCATTTGGTGGTTTTCTGGAGGTTGACGGGAAAAGGGTGGGTGTTGCCACCCTCTGCCTTGAAGAGGAA
GCGTGCAGGAAAGTTGAGGACGGGGAGGGTTATGCTGTCTACTCCCTCGACAGGCTTGGAATTCCACTGGTTGAGATAGG
CACCGAGCCAGACATCGATTCGCCAGAAATGGCAAAGAAGGTTGCAGCGAGGCTCGGGATGATTTTGAGGTCAACGGGAA
AGGTTAAGAGGGGCTTGGGGACGATAAGGCAGGATGTAAACATCAGCATAAGGGATGGGGCGAGGGTGGAGATAAAGGGC
GTGCAGGAACTCGACATTCTCGACAAGATTGTTGAATACGAGGTTTTGAGGCAGGTCAACCTTCTCAAAATAAGGGAGGA
GCTCAAGCAGAGGGGAGCGAGGGTAGAGGAGAAGGTTTTTGATGTCACAGACGTTTTCAGCAACACGAAGTCGAAGATAA
TCAGAAACAAGACGGTGAAGGCAATTTTGCTGAGAGGTTTTGGAGGAATTGTCGGGAGAGAAATCCAGCCCGGAAGGAGG
CTCGGAACGGAGTTCGCAGACATCGCCAAGACCTACGGGCTTGGGGGAGTATTCCACACTGATGAGCTGCCTGCTTACGG
GATAAGCGAGGAAGAGGTGAGCAGGCTGAGAGATGCTGTGGGCGCTGAAGACGGCGATGCGGTTGTAATGGCGGCAGGAG
ATGCGGTGAGAGTTGAGAGGGCCTTGAGAAGGATAATAGAGAGGGCAAAACACTGCCTTGTTGGAGTTCCTGAGGAGACA
AGGAAGGCTAACGAGGATGGGACGACCTCTTACCTCCGCCCATTGCCGGGAGCGGCAAGAATGTATCCCGAAACGGATGT
GCCTCCCGTCGTTGTGACCGAGGAGATGCTGAACGTGGAGATTCCAGAGCTAATAGAGGAAAGGGCGAGGAGGTACGAGA
AGCTCCTGCCAAAGGACCTTGCATGGGAGATTGCAGATTCGCCATATTATAGAGTTTTTGAGGAGTATTCTGAGAAGCTG
CAGCCAACGGTTGTTGCGAGGGTGCTTTACCTTGCACCGGCTGCACTGAGGAGAGAAGGAGTAGAAATTGAAAGGCTTGA
GGAGAGGCATTACAGGTTAGTTCTCGACATGGTTCTTAGAGGGGACATAGCAAAGGAGGGAGCGGAGGAGGCTTTAAAGC
TCCTCTGTCAGAATCCGGAAATGTCTGCTGGGCAGTTGAAGGAGAAAATAGGTGCTGCAGAGGACATCGACGGCTTCATT
GCAAAGCTTATTGAGGAAAAGGCCGATTTGATTGCTGAGAGGGGAGAGGGGGCATTTAAGCCTCTCATGGGACTCGTGAT
GAAAGAGTTCAGGGGCAAGGTCGATGGAAAGGTGGTGGCTGAGAAGCTGAAGAAGGCCCTGAAGAGCGCGCTCTCTCAGT
AG

Upstream 100 bases:

>100_bases
GGGTGTTTACAGGCTACCATCTTCCCACAATAGCGCCTGAAGCTTACGTGGTGGATGTCAGAAGTGAGAACTACCTCCGA
AAGATTACGCTTAAGGTGAG

Downstream 100 bases:

>100_bases
TTTTTTAATAACTCATAGTTGAAGGGTATCCGTGGCGATATACGCATTCCCCGAGTTCGACGGAGAAAGCAGAAGAGCGA
GCAGAATTTACGCAAGTGCA

Product: glutamyl-tRNA(Gln) amidotransferase subunit E

Products: NA

Alternate protein names: Glu-ADT subunit E

Number of amino acids: Translated: 613; Mature: 613

Protein sequence:

>613_residues
MMDYKKLGLKVGIEIHQQLDTKHKLFCLCPTLTREVEESNFEFFRYLRLKRSEIGEEDRAAKEEVERSRRFIYKYYDTTC
LVEADEEPPREVNREALLIAIQVAKMLNMEVVDEVHVMRKIVIDGSNTTGFQRTALVAFGGFLEVDGKRVGVATLCLEEE
ACRKVEDGEGYAVYSLDRLGIPLVEIGTEPDIDSPEMAKKVAARLGMILRSTGKVKRGLGTIRQDVNISIRDGARVEIKG
VQELDILDKIVEYEVLRQVNLLKIREELKQRGARVEEKVFDVTDVFSNTKSKIIRNKTVKAILLRGFGGIVGREIQPGRR
LGTEFADIAKTYGLGGVFHTDELPAYGISEEEVSRLRDAVGAEDGDAVVMAAGDAVRVERALRRIIERAKHCLVGVPEET
RKANEDGTTSYLRPLPGAARMYPETDVPPVVVTEEMLNVEIPELIEERARRYEKLLPKDLAWEIADSPYYRVFEEYSEKL
QPTVVARVLYLAPAALRREGVEIERLEERHYRLVLDMVLRGDIAKEGAEEALKLLCQNPEMSAGQLKEKIGAAEDIDGFI
AKLIEEKADLIAERGEGAFKPLMGLVMKEFRGKVDGKVVAEKLKKALKSALSQ

Sequences:

>Translated_613_residues
MMDYKKLGLKVGIEIHQQLDTKHKLFCLCPTLTREVEESNFEFFRYLRLKRSEIGEEDRAAKEEVERSRRFIYKYYDTTC
LVEADEEPPREVNREALLIAIQVAKMLNMEVVDEVHVMRKIVIDGSNTTGFQRTALVAFGGFLEVDGKRVGVATLCLEEE
ACRKVEDGEGYAVYSLDRLGIPLVEIGTEPDIDSPEMAKKVAARLGMILRSTGKVKRGLGTIRQDVNISIRDGARVEIKG
VQELDILDKIVEYEVLRQVNLLKIREELKQRGARVEEKVFDVTDVFSNTKSKIIRNKTVKAILLRGFGGIVGREIQPGRR
LGTEFADIAKTYGLGGVFHTDELPAYGISEEEVSRLRDAVGAEDGDAVVMAAGDAVRVERALRRIIERAKHCLVGVPEET
RKANEDGTTSYLRPLPGAARMYPETDVPPVVVTEEMLNVEIPELIEERARRYEKLLPKDLAWEIADSPYYRVFEEYSEKL
QPTVVARVLYLAPAALRREGVEIERLEERHYRLVLDMVLRGDIAKEGAEEALKLLCQNPEMSAGQLKEKIGAAEDIDGFI
AKLIEEKADLIAERGEGAFKPLMGLVMKEFRGKVDGKVVAEKLKKALKSALSQ
>Mature_613_residues
MMDYKKLGLKVGIEIHQQLDTKHKLFCLCPTLTREVEESNFEFFRYLRLKRSEIGEEDRAAKEEVERSRRFIYKYYDTTC
LVEADEEPPREVNREALLIAIQVAKMLNMEVVDEVHVMRKIVIDGSNTTGFQRTALVAFGGFLEVDGKRVGVATLCLEEE
ACRKVEDGEGYAVYSLDRLGIPLVEIGTEPDIDSPEMAKKVAARLGMILRSTGKVKRGLGTIRQDVNISIRDGARVEIKG
VQELDILDKIVEYEVLRQVNLLKIREELKQRGARVEEKVFDVTDVFSNTKSKIIRNKTVKAILLRGFGGIVGREIQPGRR
LGTEFADIAKTYGLGGVFHTDELPAYGISEEEVSRLRDAVGAEDGDAVVMAAGDAVRVERALRRIIERAKHCLVGVPEET
RKANEDGTTSYLRPLPGAARMYPETDVPPVVVTEEMLNVEIPELIEERARRYEKLLPKDLAWEIADSPYYRVFEEYSEKL
QPTVVARVLYLAPAALRREGVEIERLEERHYRLVLDMVLRGDIAKEGAEEALKLLCQNPEMSAGQLKEKIGAAEDIDGFI
AKLIEEKADLIAERGEGAFKPLMGLVMKEFRGKVDGKVVAEKLKKALKSALSQ

Specific function: Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-ph

COG id: COG2511

COG function: function code J; Archaeal Glu-tRNAGln amidotransferase subunit E (contains GAD domain)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatE subfamily

Homologues:

Organism=Saccharomyces cerevisiae, GI6319391, Length=276, Percent_Identity=25, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATE_ARCFU (O28832)

Other databases:

- EMBL:   AE000782
- PIR:   G69429
- RefSeq:   NP_070269.1
- ProteinModelPortal:   O28832
- SMR:   O28832
- GeneID:   1484664
- GenomeReviews:   AE000782_GR
- KEGG:   afu:AF1440
- NMPDR:   fig|224325.1.peg.1430
- TIGR:   AF_1440
- HOGENOM:   HBG297405
- OMA:   TSGFQRT
- ProtClustDB:   PRK04028
- BioCyc:   AFUL224325:AF_1440-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00588
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004115
- InterPro:   IPR023168
- InterPro:   IPR004414
- InterPro:   IPR006075
- InterPro:   IPR017958
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:1.10.10.410
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00134

Pfam domain/function: PF02938 GAD; PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 69025; Mature: 69025

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMDYKKLGLKVGIEIHQQLDTKHKLFCLCPTLTREVEESNFEFFRYLRLKRSEIGEEDRA
CCCHHHHCHHHHHHHHHHCCCCCCEEEECHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHH
AKEEVERSRRFIYKYYDTTCLVEADEEPPREVNREALLIAIQVAKMLNMEVVDEVHVMRK
HHHHHHHHHHHHHHEECCEEEEECCCCCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVIDGSNTTGFQRTALVAFGGFLEVDGKRVGVATLCLEEEACRKVEDGEGYAVYSLDRLG
HHCCCCCCCCHHHHHHHHCCCEEEECCCEECHHHHHCCHHHHHCCCCCCCEEEEEEHHCC
IPLVEIGTEPDIDSPEMAKKVAARLGMILRSTGKVKRGLGTIRQDVNISIRDGARVEIKG
CCEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEECCCCEEEECC
VQELDILDKIVEYEVLRQVNLLKIREELKQRGARVEEKVFDVTDVFSNTKSKIIRNKTVK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AILLRGFGGIVGREIQPGRRLGTEFADIAKTYGLGGVFHTDELPAYGISEEEVSRLRDAV
HHHHHHHCCCCCCCCCCCHHHCHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHH
GAEDGDAVVMAAGDAVRVERALRRIIERAKHCLVGVPEETRKANEDGTTSYLRPLPGAAR
CCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCHHHHCCCCCCCC
MYPETDVPPVVVTEEMLNVEIPELIEERARRYEKLLPKDLAWEIADSPYYRVFEEYSEKL
CCCCCCCCCEEEEHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHH
QPTVVARVLYLAPAALRREGVEIERLEERHYRLVLDMVLRGDIAKEGAEEALKLLCQNPE
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
MSAGQLKEKIGAAEDIDGFIAKLIEEKADLIAERGEGAFKPLMGLVMKEFRGKVDGKVVA
CCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHH
EKLKKALKSALSQ
HHHHHHHHHHHCC
>Mature Secondary Structure
MMDYKKLGLKVGIEIHQQLDTKHKLFCLCPTLTREVEESNFEFFRYLRLKRSEIGEEDRA
CCCHHHHCHHHHHHHHHHCCCCCCEEEECHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHH
AKEEVERSRRFIYKYYDTTCLVEADEEPPREVNREALLIAIQVAKMLNMEVVDEVHVMRK
HHHHHHHHHHHHHHEECCEEEEECCCCCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVIDGSNTTGFQRTALVAFGGFLEVDGKRVGVATLCLEEEACRKVEDGEGYAVYSLDRLG
HHCCCCCCCCHHHHHHHHCCCEEEECCCEECHHHHHCCHHHHHCCCCCCCEEEEEEHHCC
IPLVEIGTEPDIDSPEMAKKVAARLGMILRSTGKVKRGLGTIRQDVNISIRDGARVEIKG
CCEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEECCCCEEEECC
VQELDILDKIVEYEVLRQVNLLKIREELKQRGARVEEKVFDVTDVFSNTKSKIIRNKTVK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AILLRGFGGIVGREIQPGRRLGTEFADIAKTYGLGGVFHTDELPAYGISEEEVSRLRDAV
HHHHHHHCCCCCCCCCCCHHHCHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHH
GAEDGDAVVMAAGDAVRVERALRRIIERAKHCLVGVPEETRKANEDGTTSYLRPLPGAAR
CCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCHHHHCCCCCCCC
MYPETDVPPVVVTEEMLNVEIPELIEERARRYEKLLPKDLAWEIADSPYYRVFEEYSEKL
CCCCCCCCCEEEEHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHH
QPTVVARVLYLAPAALRREGVEIERLEERHYRLVLDMVLRGDIAKEGAEEALKLLCQNPE
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
MSAGQLKEKIGAAEDIDGFIAKLIEEKADLIAERGEGAFKPLMGLVMKEFRGKVDGKVVA
CCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHH
EKLKKALKSALSQ
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475