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The map label for this gene is asnB

Identifier: 11499034

GI number: 11499034

Start: 1296130

End: 1297245

Strand: Reverse

Name: asnB

Synonym: AF1439

Alternate gene names: 11499034

Gene position: 1297245-1296130 (Counterclockwise)

Preceding gene: 11499035

Following gene: 11499033

Centisome position: 59.55

GC content: 50.0

Gene sequence:

>1116_bases
GTGGCGATATACGCATTCCCCGAGTTCGACGGAGAAAGCAGAAGAGCGAGCAGAATTTACGCAAGTGCAAGATATCCATC
AAGGCAGGAGGGAAACGTCTTCTTCGACTCGGAAGAGGTTCCGAATCTGAAGTATGCGGACGAGATGCCTCTTAAAATCA
GAAATTTTTACTCAGTTGTTGCCTTCCGCCCGTCCCACATTTTTCTTTCCAGAGACGTGCTCGGCGGGAGGCCCCTCTAC
TACGGCAGCGACATGAGCATTTCGTCCTTCAAATCCTACATTGACGGAGAGGTTTACGAGGTGATGCCGGGAGAAGTGGT
CAAGATGGACTATACCGGCGAAATTCTGGAAAGGAGAAAGTATGCCTTCGAGGACGTCTTCAGAGTTGGCCGGGAAGACG
TTAACGAAATGGCAGACAGAATTCTGAACTCCCTTAGCAGCTACAAGCCGAGAGTTGGGTGCATAGCCTTTTCTGGAGGG
GTTGATTCATCCCTGCTCGCCGCTCTTTACGATGCCCCACTCGTTTCTGTAACAGCAAGCGATGCAGAGGAGGAGTGGCT
TAAAACCGCCGCAAGAATGCTCGGAAAGGAGATTGAAATACTGAAGGTGGGAGAGAGTGCGATAAGGGAAGTTGTGGATA
CGGTGAGAAAAACAATAGAGACGGGCGATTTTCTGCAGCTTTCCATAGCCGTTCCGGTTTACCTAACCATGCAGTTCGCC
AAATCCCTTGGCTTCACGGAGATCATTTTCGGTCAGGGAGCGGACGAGCTTTTTGGTGGGTACAAAAGGTACGAGGGTCT
GAGTGAGCACGAGCTTGAGGACGCCTTGGTGAAGGACTTGAAGTCCATTGGTGAGTCAAATCTCGTGAGGGATTGCAAGC
TGGCTTACAAGAACGAGATCAAGCTGGTAACGCCTTACTTGTGCTGGGATGTAATTGATGCGGCCCTGAAAATTCCTCCT
GAGTACAAGGTCAGAAGGGAGGGTGGGAAGGTAGTCAGGAAGTACATTCTGAGAAAAATTGCCGAGAAATTCCTTCCGGA
GGAGATAGCGTGGAGGGACAAGAAGGCGATTCAGTACTCAACAGGAATAGCGAAAATTCTGAAGAAGTGTCTGTGA

Upstream 100 bases:

>100_bases
GGCAAGGTCGATGGAAAGGTGGTGGCTGAGAAGCTGAAGAAGGCCCTGAAGAGCGCGCTCTCTCAGTAGTTTTTTAATAA
CTCATAGTTGAAGGGTATCC

Downstream 100 bases:

>100_bases
ACTGCTTCGACGGCTCTTCCTTCTGCGGAAAATGCTGCTACGAGACGGAGATGCCGCTTACAGAAGAGGACATTGCAAGG
ATAGAAGCTTTGGGCTATTC

Product: asparagine synthetase (asnB)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 371; Mature: 370

Protein sequence:

>371_residues
MAIYAFPEFDGESRRASRIYASARYPSRQEGNVFFDSEEVPNLKYADEMPLKIRNFYSVVAFRPSHIFLSRDVLGGRPLY
YGSDMSISSFKSYIDGEVYEVMPGEVVKMDYTGEILERRKYAFEDVFRVGREDVNEMADRILNSLSSYKPRVGCIAFSGG
VDSSLLAALYDAPLVSVTASDAEEEWLKTAARMLGKEIEILKVGESAIREVVDTVRKTIETGDFLQLSIAVPVYLTMQFA
KSLGFTEIIFGQGADELFGGYKRYEGLSEHELEDALVKDLKSIGESNLVRDCKLAYKNEIKLVTPYLCWDVIDAALKIPP
EYKVRREGGKVVRKYILRKIAEKFLPEEIAWRDKKAIQYSTGIAKILKKCL

Sequences:

>Translated_371_residues
MAIYAFPEFDGESRRASRIYASARYPSRQEGNVFFDSEEVPNLKYADEMPLKIRNFYSVVAFRPSHIFLSRDVLGGRPLY
YGSDMSISSFKSYIDGEVYEVMPGEVVKMDYTGEILERRKYAFEDVFRVGREDVNEMADRILNSLSSYKPRVGCIAFSGG
VDSSLLAALYDAPLVSVTASDAEEEWLKTAARMLGKEIEILKVGESAIREVVDTVRKTIETGDFLQLSIAVPVYLTMQFA
KSLGFTEIIFGQGADELFGGYKRYEGLSEHELEDALVKDLKSIGESNLVRDCKLAYKNEIKLVTPYLCWDVIDAALKIPP
EYKVRREGGKVVRKYILRKIAEKFLPEEIAWRDKKAIQYSTGIAKILKKCL
>Mature_370_residues
AIYAFPEFDGESRRASRIYASARYPSRQEGNVFFDSEEVPNLKYADEMPLKIRNFYSVVAFRPSHIFLSRDVLGGRPLYY
GSDMSISSFKSYIDGEVYEVMPGEVVKMDYTGEILERRKYAFEDVFRVGREDVNEMADRILNSLSSYKPRVGCIAFSGGV
DSSLLAALYDAPLVSVTASDAEEEWLKTAARMLGKEIEILKVGESAIREVVDTVRKTIETGDFLQLSIAVPVYLTMQFAK
SLGFTEIIFGQGADELFGGYKRYEGLSEHELEDALVKDLKSIGESNLVRDCKLAYKNEIKLVTPYLCWDVIDAALKIPPE
YKVRREGGKVVRKYILRKIAEKFLPEEIAWRDKKAIQYSTGIAKILKKCL

Specific function: Asparagine biosynthesis. [C]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1786889, Length=335, Percent_Identity=27.7611940298507, Blast_Score=85, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17560178, Length=336, Percent_Identity=25.5952380952381, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI25147557, Length=231, Percent_Identity=23.8095238095238, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI71993933, Length=231, Percent_Identity=23.8095238095238, Blast_Score=72, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6321563, Length=164, Percent_Identity=28.0487804878049, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6325403, Length=164, Percent_Identity=28.0487804878049, Blast_Score=68, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323542, Length=381, Percent_Identity=23.6220472440945, Blast_Score=67, Evalue=5e-12,
Organism=Drosophila melanogaster, GI45553209, Length=338, Percent_Identity=28.1065088757396, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 42074; Mature: 41943

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIYAFPEFDGESRRASRIYASARYPSRQEGNVFFDSEEVPNLKYADEMPLKIRNFYSVV
CEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHE
AFRPSHIFLSRDVLGGRPLYYGSDMSISSFKSYIDGEVYEVMPGEVVKMDYTGEILERRK
EECCCEEEEECCCCCCCCEEECCCCCHHHHHHHHCCCEEEECCCCEEEECCCHHHHHHHH
YAFEDVFRVGREDVNEMADRILNSLSSYKPRVGCIAFSGGVDSSLLAALYDAPLVSVTAS
HHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHCCCEEEEECC
DAEEEWLKTAARMLGKEIEILKVGESAIREVVDTVRKTIETGDFLQLSIAVPVYLTMQFA
CHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHH
KSLGFTEIIFGQGADELFGGYKRYEGLSEHELEDALVKDLKSIGESNLVRDCKLAYKNEI
HHCCCEEEEECCCHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCC
KLVTPYLCWDVIDAALKIPPEYKVRREGGKVVRKYILRKIAEKFLPEEIAWRDKKAIQYS
EEECHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHH
TGIAKILKKCL
HHHHHHHHHHC
>Mature Secondary Structure 
AIYAFPEFDGESRRASRIYASARYPSRQEGNVFFDSEEVPNLKYADEMPLKIRNFYSVV
EEEECCCCCCCCHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHE
AFRPSHIFLSRDVLGGRPLYYGSDMSISSFKSYIDGEVYEVMPGEVVKMDYTGEILERRK
EECCCEEEEECCCCCCCCEEECCCCCHHHHHHHHCCCEEEECCCCEEEECCCHHHHHHHH
YAFEDVFRVGREDVNEMADRILNSLSSYKPRVGCIAFSGGVDSSLLAALYDAPLVSVTAS
HHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHCCCEEEEECC
DAEEEWLKTAARMLGKEIEILKVGESAIREVVDTVRKTIETGDFLQLSIAVPVYLTMQFA
CHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHH
KSLGFTEIIFGQGADELFGGYKRYEGLSEHELEDALVKDLKSIGESNLVRDCKLAYKNEI
HHCCCEEEEECCCHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCC
KLVTPYLCWDVIDAALKIPPEYKVRREGGKVVRKYILRKIAEKFLPEEIAWRDKKAIQYS
EEECHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHH
TGIAKILKKCL
HHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]