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The map label for this gene is glpF

Identifier: 11499021

GI number: 11499021

Start: 1287436

End: 1288176

Strand: Reverse

Name: glpF

Synonym: AF1426

Alternate gene names: 11499021

Gene position: 1288176-1287436 (Counterclockwise)

Preceding gene: 11499022

Following gene: 11499018

Centisome position: 59.13

GC content: 54.93

Gene sequence:

>741_bases
GTGACTATGACTCTTGCCAAGCGCTTTACCGCTGAGGTTGTTGGAACCTTTATCCTTGTCTTCTTTGGCCCTGGTGCGGC
AGTAATTACGCTGATGATTGCAAATGGGGCAGATAAGCCCAACGAGTTCAACATCGGCATTGGAGCTCTCGGCGGTCTTG
GAGACTGGTTCGCCATAGGAATGGCCTTCGCTCTGGCGATTGCAGCAGTCATCTACTCTCTCGGAAGAATCAGCGGTGCT
CACATCAATCCGGCAGTGACGATAGCTCTGTGGAGCATCGGGAGGTTTCCGGGGAGAGAGGTGGTTCCATACATAGTGGC
ACAGTTTATTGGTGCAGCACTTGGAAGCCTGCTGTTCCTCGCCTGTGTGGGGCCTGCAGCAGCAACGGTTGGAGGCCTGG
GAGCGACTGCACCATTTCCGGGAATAGGCTACGGACAGGCGATACTGACGGAAGCTATCGGAACTTTCCTGCTGATGCTG
GTTATAATGGGAGTTGCGGTTGACGAGCGCGCCCCTCCGGGATTTGCCGGGCTTGTCATAGGTCTGACGGTTGGAGGAAT
CATCACGACCATCGGTAACATTACGGGCTCATCGCTCAACCCCGCGAGAACCTTCGGGCCATACCTTGGAGACTCGCTGA
TGGGGATAAACCTCTGGCAGTACTTCCCGATTTACGTCATCGGGCCGATTGTTGGTGCTGTAGCTGCGGCATGGCTGTAC
AACTATCTGGCCAAAGAGTAA

Upstream 100 bases:

>100_bases
TTCCTCTCTGGAAGGGAGACTATCCCTTCCCCGCAAGGAAGATGAAGAAGACCGTGCCGAGAATTCTGTGTGTTATCTGC
CATGAAGCTTACCAGGAGGA

Downstream 100 bases:

>100_bases
CCTATTTTTTTATAAGTAAGTCCATAAGCCTTAAACCATCCACAAGGCCGAATTTTCCCCTTCTGCAGCTTTTTTCCGTG
TAAACCTTAACCTCATCTCT

Product: glycerol uptake facilitator, MIP channel (glpF)

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MTMTLAKRFTAEVVGTFILVFFGPGAAVITLMIANGADKPNEFNIGIGALGGLGDWFAIGMAFALAIAAVIYSLGRISGA
HINPAVTIALWSIGRFPGREVVPYIVAQFIGAALGSLLFLACVGPAAATVGGLGATAPFPGIGYGQAILTEAIGTFLLML
VIMGVAVDERAPPGFAGLVIGLTVGGIITTIGNITGSSLNPARTFGPYLGDSLMGINLWQYFPIYVIGPIVGAVAAAWLY
NYLAKE

Sequences:

>Translated_246_residues
MTMTLAKRFTAEVVGTFILVFFGPGAAVITLMIANGADKPNEFNIGIGALGGLGDWFAIGMAFALAIAAVIYSLGRISGA
HINPAVTIALWSIGRFPGREVVPYIVAQFIGAALGSLLFLACVGPAAATVGGLGATAPFPGIGYGQAILTEAIGTFLLML
VIMGVAVDERAPPGFAGLVIGLTVGGIITTIGNITGSSLNPARTFGPYLGDSLMGINLWQYFPIYVIGPIVGAVAAAWLY
NYLAKE
>Mature_245_residues
TMTLAKRFTAEVVGTFILVFFGPGAAVITLMIANGADKPNEFNIGIGALGGLGDWFAIGMAFALAIAAVIYSLGRISGAH
INPAVTIALWSIGRFPGREVVPYIVAQFIGAALGSLLFLACVGPAAATVGGLGATAPFPGIGYGQAILTEAIGTFLLMLV
IMGVAVDERAPPGFAGLVIGLTVGGIITTIGNITGSSLNPARTFGPYLGDSLMGINLWQYFPIYVIGPIVGAVAAAWLYN
YLAKE

Specific function: Channel that permits osmotically driven movement of water in both directions

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family

Homologues:

Organism=Homo sapiens, GI4755125, Length=192, Percent_Identity=40.625, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI4502181, Length=192, Percent_Identity=40.625, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI157266307, Length=265, Percent_Identity=29.4339622641509, Blast_Score=88, Evalue=8e-18,
Organism=Homo sapiens, GI4502183, Length=239, Percent_Identity=36.4016736401674, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI4502179, Length=245, Percent_Identity=33.469387755102, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI4826645, Length=266, Percent_Identity=28.9473684210526, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI22538420, Length=265, Percent_Identity=27.9245283018868, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI37694062, Length=191, Percent_Identity=32.4607329842932, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI6912506, Length=194, Percent_Identity=32.9896907216495, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI4502187, Length=258, Percent_Identity=27.5193798449612, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI45446752, Length=174, Percent_Identity=34.4827586206897, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI86792455, Length=189, Percent_Identity=31.7460317460317, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1790362, Length=272, Percent_Identity=32.7205882352941, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787101, Length=209, Percent_Identity=37.3205741626794, Blast_Score=97, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI71994009, Length=260, Percent_Identity=34.2307692307692, Blast_Score=94, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17558372, Length=197, Percent_Identity=34.010152284264, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71993722, Length=184, Percent_Identity=33.695652173913, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17531431, Length=260, Percent_Identity=30, Blast_Score=81, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17531429, Length=260, Percent_Identity=30, Blast_Score=80, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI32564052, Length=262, Percent_Identity=29.3893129770992, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17533613, Length=262, Percent_Identity=29.3893129770992, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI71992966, Length=261, Percent_Identity=29.1187739463602, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17544068, Length=219, Percent_Identity=28.310502283105, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6321054, Length=218, Percent_Identity=32.1100917431193, Blast_Score=80, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24652747, Length=235, Percent_Identity=31.063829787234, Blast_Score=93, Evalue=1e-19,
Organism=Drosophila melanogaster, GI45551084, Length=235, Percent_Identity=31.063829787234, Blast_Score=93, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24762344, Length=248, Percent_Identity=30.241935483871, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24762346, Length=248, Percent_Identity=30.241935483871, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24762348, Length=248, Percent_Identity=30.241935483871, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI20130305, Length=248, Percent_Identity=30.241935483871, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24762342, Length=204, Percent_Identity=32.843137254902, Blast_Score=77, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24652751, Length=232, Percent_Identity=32.3275862068966, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI19922038, Length=241, Percent_Identity=31.5352697095436, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI45550503, Length=239, Percent_Identity=26.7782426778243, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AQPM_ARCFU (O28846)

Other databases:

- EMBL:   AE000782
- PIR:   A69428
- RefSeq:   NP_070255.1
- PDB:   3NE2
- PDBsum:   3NE2
- ProteinModelPortal:   O28846
- SMR:   O28846
- GeneID:   1484650
- GenomeReviews:   AE000782_GR
- KEGG:   afu:AF1426
- NMPDR:   fig|224325.1.peg.1416
- TIGR:   AF_1426
- HOGENOM:   HBG705794
- OMA:   LNPARTF
- ProtClustDB:   CLSK862689
- BioCyc:   AFUL224325:AF_1426-MONOMER
- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357
- PANTHER:   PTHR19139
- PRINTS:   PR00783
- TIGRFAMs:   TIGR00861

Pfam domain/function: PF00230 MIP

EC number: NA

Molecular weight: Translated: 25212; Mature: 25081

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x45274208)-; HASH(0x452fa0a4)-; HASH(0x452155c4)-; HASH(0x452fab24)-; HASH(0x452f2ab4)-; HASH(0x452f9d08)-;

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHCH
>Mature Secondary Structure 
TMTLAKRFTAEVVGTFILVFFGPGAAVITLMIANGADKPNEFNIGIGALGGLGDWFAIG
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCEECCCCCCCCCHHHHHHH
MAFALAIAAVIYSLGRISGAHINPAVTIALWSIGRFPGREVVPYIVAQFIGAALGSLLFL
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
ACVGPAAATVGGLGATAPFPGIGYGQAILTEAIGTFLLMLVIMGVAVDERAPPGFAGLVI
HHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
GLTVGGIITTIGNITGSSLNPARTFGPYLGDSLMGINLWQYFPIYVIGPIVGAVAAAWLY
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NYLAKE
HHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9389475