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The map label for this gene is prsA-2

Identifier: 11499014

GI number: 11499014

Start: 1278165

End: 1278980

Strand: Reverse

Name: prsA-2

Synonym: AF1419

Alternate gene names: 11499014

Gene position: 1278980-1278165 (Counterclockwise)

Preceding gene: 11499015

Following gene: 11499013

Centisome position: 58.71

GC content: 49.75

Gene sequence:

>816_bases
ATGAAAATAATTCCGTGTCCGTCTTCTCCGCTGCTGGCGAGGAGGGTTGCTGAAGCCGCAGGAATTGAGATTGGAGGGGC
GGTTTTCAAAAAATTTCCCGATGGAGAGCTTTACGTGAGGGTGATGGAGAAAGAAGGGGTGGTTGTGGGAAGCATAAACT
CCAATGAAGACCTCATTGCCCTAATCTTCGCCTTGGATGTTCTGGAAAATGCCAAGGCTGTGGTTCCATACATGGGCTAC
GCAAGGCAGGACAAGGTGTTTCAGGAAGGAGAAGCTGTGAGCATTAAAATTGTGGCTGAGATGCTGGAAAGCAGGGCTGA
CGAAGTTGTTACTGTAAACATCCACAGCAGCGATGCGGCATCGCACTTCAGCAAGCTGAAGAACCTCGATGCAATGCCCT
TGGTTGGTGAGTACTTTGCAGGGAAGGATGTTGTTATGATTTCCCCCGATAAAGGCTCGATGGAGAGAGTAAAGACTGCT
GCAAAGCATGCAGGATGTGAGTGGGACTACATGGAGAAGCGCAGGATTGACGCCACAACTGTCGAAATCACTCCCAAGAC
AATCGACGTTGAGGGAAGAGACGTTGTCATTGTGGACGACATAATCTCCACTGGCGGCACAGTTGCTGAGGCTGCGAGAA
TTCTTTACGGTCTTGGAGCTAAAAGCGTCAGTGCGGCATGCGTTCACGCAGTTCTGGCTGAGAATGCTGCAATAAAGCTC
TTCAATGCCGGAATTAAGGACATCATCGCTACCGACACTGTTGAGTGTGCCTTCAGCAGGATAAGCGTTGCTGAGCTTAT
AGCGGAGAATATCTGA

Upstream 100 bases:

>100_bases
GGAGCAGAACACGACCATCGTGATGCCCATTCCTGTGGAGCTTCTGAAGTTCTTCGTGGAGAAAGCTAAAAGTTAAAAGC
CCTTCCTCTTTTTTTGATTC

Downstream 100 bases:

>100_bases
AAAACTTTTATGCCTACCTGAATTTGGATTGGAGATGAAGAAAAGGGTCGTGGCTACAGGGACTTTCGACATAATCCATC
CGGGACACATAACCTTCCTC

Product: ribose-phosphate pyrophosphokinase (prsA-2)

Products: NA

Alternate protein names: RPPK 2; Phosphoribosyl pyrophosphate synthase 2; P-Rib-PP synthase 2; PRPP synthase 2

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MKIIPCPSSPLLARRVAEAAGIEIGGAVFKKFPDGELYVRVMEKEGVVVGSINSNEDLIALIFALDVLENAKAVVPYMGY
ARQDKVFQEGEAVSIKIVAEMLESRADEVVTVNIHSSDAASHFSKLKNLDAMPLVGEYFAGKDVVMISPDKGSMERVKTA
AKHAGCEWDYMEKRRIDATTVEITPKTIDVEGRDVVIVDDIISTGGTVAEAARILYGLGAKSVSAACVHAVLAENAAIKL
FNAGIKDIIATDTVECAFSRISVAELIAENI

Sequences:

>Translated_271_residues
MKIIPCPSSPLLARRVAEAAGIEIGGAVFKKFPDGELYVRVMEKEGVVVGSINSNEDLIALIFALDVLENAKAVVPYMGY
ARQDKVFQEGEAVSIKIVAEMLESRADEVVTVNIHSSDAASHFSKLKNLDAMPLVGEYFAGKDVVMISPDKGSMERVKTA
AKHAGCEWDYMEKRRIDATTVEITPKTIDVEGRDVVIVDDIISTGGTVAEAARILYGLGAKSVSAACVHAVLAENAAIKL
FNAGIKDIIATDTVECAFSRISVAELIAENI
>Mature_271_residues
MKIIPCPSSPLLARRVAEAAGIEIGGAVFKKFPDGELYVRVMEKEGVVVGSINSNEDLIALIFALDVLENAKAVVPYMGY
ARQDKVFQEGEAVSIKIVAEMLESRADEVVTVNIHSSDAASHFSKLKNLDAMPLVGEYFAGKDVVMISPDKGSMERVKTA
AKHAGCEWDYMEKRRIDATTVEITPKTIDVEGRDVVIVDDIISTGGTVAEAARILYGLGAKSVSAACVHAVLAENAAIKL
FNAGIKDIIATDTVECAFSRISVAELIAENI

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family

Homologues:

Organism=Homo sapiens, GI4506129, Length=261, Percent_Identity=32.183908045977, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI84875539, Length=264, Percent_Identity=32.5757575757576, Blast_Score=113, Evalue=1e-25,
Organism=Homo sapiens, GI4506127, Length=272, Percent_Identity=30.5147058823529, Blast_Score=107, Evalue=9e-24,
Organism=Homo sapiens, GI28557709, Length=272, Percent_Identity=29.4117647058824, Blast_Score=101, Evalue=7e-22,
Organism=Escherichia coli, GI1787458, Length=274, Percent_Identity=32.8467153284672, Blast_Score=125, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI25149168, Length=272, Percent_Identity=30.8823529411765, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17554702, Length=272, Percent_Identity=30.8823529411765, Blast_Score=108, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17554704, Length=265, Percent_Identity=31.3207547169811, Blast_Score=107, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI71989924, Length=272, Percent_Identity=30.8823529411765, Blast_Score=107, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6321776, Length=286, Percent_Identity=29.020979020979, Blast_Score=100, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6320946, Length=273, Percent_Identity=27.8388278388278, Blast_Score=97, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6319403, Length=274, Percent_Identity=28.1021897810219, Blast_Score=96, Evalue=7e-21,
Organism=Drosophila melanogaster, GI21355239, Length=272, Percent_Identity=30.8823529411765, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI45551540, Length=295, Percent_Identity=28.8135593220339, Blast_Score=100, Evalue=1e-21,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): KPRS2_ARCFU (O28853)

Other databases:

- EMBL:   AE000782
- PIR:   B69427
- RefSeq:   NP_070248.1
- ProteinModelPortal:   O28853
- SMR:   O28853
- GeneID:   1484643
- GenomeReviews:   AE000782_GR
- KEGG:   afu:AF1419
- NMPDR:   fig|224325.1.peg.1409
- TIGR:   AF_1419
- HOGENOM:   HBG519284
- OMA:   FPDGEIY
- ProtClustDB:   CLSK2746896
- BioCyc:   AFUL224325:AF_1419-MONOMER
- BRENDA:   2.7.6.1
- GO:   GO:0005737
- HAMAP:   MF_00583_A
- InterPro:   IPR005946
- InterPro:   IPR000836
- TIGRFAMs:   TIGR01251

Pfam domain/function: PF00156 Pribosyltran

EC number: =2.7.6.1

Molecular weight: Translated: 29059; Mature: 29059

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00114 PRPP_SYNTHASE; PS00103 PUR_PYR_PR_TRANSFER ; PS00144 ASN_GLN_ASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIIPCPSSPLLARRVAEAAGIEIGGAVFKKFPDGELYVRVMEKEGVVVGSINSNEDLIA
CEEECCCCCHHHHHHHHHHCCCEECHHHHHCCCCCEEEEEEEECCCEEEEECCCCCCHHH
LIFALDVLENAKAVVPYMGYARQDKVFQEGEAVSIKIVAEMLESRADEVVTVNIHSSDAA
HHHHHHHHHCCCEEECCCCCCHHCHHHHCCCEEEHHHHHHHHHCCCCCEEEEEEECCHHH
SHFSKLKNLDAMPLVGEYFAGKDVVMISPDKGSMERVKTAAKHAGCEWDYMEKRRIDATT
HHHHHHHCCCCCCCHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCCCCCHHHHHCCCCEE
VEITPKTIDVEGRDVVIVDDIISTGGTVAEAARILYGLGAKSVSAACVHAVLAENAAIKL
EEEECCEEECCCCEEEEEEHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEE
FNAGIKDIIATDTVECAFSRISVAELIAENI
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKIIPCPSSPLLARRVAEAAGIEIGGAVFKKFPDGELYVRVMEKEGVVVGSINSNEDLIA
CEEECCCCCHHHHHHHHHHCCCEECHHHHHCCCCCEEEEEEEECCCEEEEECCCCCCHHH
LIFALDVLENAKAVVPYMGYARQDKVFQEGEAVSIKIVAEMLESRADEVVTVNIHSSDAA
HHHHHHHHHCCCEEECCCCCCHHCHHHHCCCEEEHHHHHHHHHCCCCCEEEEEEECCHHH
SHFSKLKNLDAMPLVGEYFAGKDVVMISPDKGSMERVKTAAKHAGCEWDYMEKRRIDATT
HHHHHHHCCCCCCCHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCCCCCHHHHHCCCCEE
VEITPKTIDVEGRDVVIVDDIISTGGTVAEAARILYGLGAKSVSAACVHAVLAENAAIKL
EEEECCEEECCCCEEEEEEHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEE
FNAGIKDIIATDTVECAFSRISVAELIAENI
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475