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The map label for this gene is hemU-2

Identifier: 11498997

GI number: 11498997

Start: 1252908

End: 1253894

Strand: Reverse

Name: hemU-2

Synonym: AF1402

Alternate gene names: 11498997

Gene position: 1253894-1252908 (Counterclockwise)

Preceding gene: 11498998

Following gene: 11498996

Centisome position: 57.56

GC content: 52.28

Gene sequence:

>987_bases
ATGACCAATTCTTTTGACTTCGTGAAGGGTAAAGTTGTAGCAGCCCTGCTCGCACTGAATCTTCTCGCTCTGCTGCTCTC
CCTTGGAGTGGGGAGCGGGGGCTATACATACCTCTTTCAGGTTATTACGGGAAATGCCGATGAGACTGCAACAAAAATCG
TTCTCAGCTACAGACTGCCGAGGTATCTGATGGCGGTGATAGCCGGAGCGTCTCTCTCTGCATCCGGCTGTGCCATGCAG
GCTCTGTTCAGAAATCCACTTGCCGAGCCCTACGTTCTTGGGGTGGCGAGCGGGGCGAGTGTTGGCGCTGCCCTTTCGGT
TGCTCTTGGCATTGAGACTATTTACTCAAGAATCGGAATGGCCTTTTTCTCCGCTCTTATTACCGCATACGTCGTTTATC
TAATTGGCTCATCCTCAAAATTTCGGGATCAGACGTACGCAATTCTTCTTGCCGGGGTTGCCATAGCAAGCTTCTTCTCC
GGTTTAACTTCCGTTCTAATTTACTTCTCCGCTCAAAGCATGCACAAAGTGATTTTCTGGCTCATGGGAAGCTTCTCGAA
CCCCTTCGTTGAAGAGGTGCTTTTCGCCCTGCCCATTACCATTGCCGGGCTGCTCTACCTCCTGATTACCTCATGGAACC
TTAACGCCCTTTTGCTCGGCGATGAGCACGCTGCAGCGGTGGGGATAAACGTCGAAAGCTTCAGGAAGTCCCTGATAGCT
GTCGTTGCTCTTCTTACTTCAACAACCGTTGCGATCAGCGGTGTCATAGGCTTTGTTGGCATAATCGTTCCCCACACAAT
GAGGATACTGGTGGGAGAGGAGCACCAGAAGCTTCTCCCCTCCACAATATTCCTCTCCACGGCTTTCATGCCGCTCATCG
ACGTCGTTGCCAGAATTTCAACGAGCGGTGAGCTGCCTGTGGGGGCGATAACGGCCATGCTCGGAGGGCCGTTTTTCCTC
TACCTGCTGATGAGGAGGTCGGTATGA

Upstream 100 bases:

>100_bases
ATGGCGGGAACGCTGGCTGTGATGCACATAAGCGAGAAGGCGAAGTTTGACCTTGACAGGGTGAAGGGCAGGAGGATTTA
GTTCGGAAAAAGTTTATCTG

Downstream 100 bases:

>100_bases
GGCTCGTGGTGAAGGGAGTCAGCGTAAAGCTGGGGAGCAGAGAGGTGCTGAAGGACGTAACCTTTGAGGTGGGGGAGGGA
GAGCTTCTCGCCCTGCTCGG

Product: iron (III) ABC transporter, permease protein (hemU-2)

Products: ADP; phosphate; cob(I)alamin [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MTNSFDFVKGKVVAALLALNLLALLLSLGVGSGGYTYLFQVITGNADETATKIVLSYRLPRYLMAVIAGASLSASGCAMQ
ALFRNPLAEPYVLGVASGASVGAALSVALGIETIYSRIGMAFFSALITAYVVYLIGSSSKFRDQTYAILLAGVAIASFFS
GLTSVLIYFSAQSMHKVIFWLMGSFSNPFVEEVLFALPITIAGLLYLLITSWNLNALLLGDEHAAAVGINVESFRKSLIA
VVALLTSTTVAISGVIGFVGIIVPHTMRILVGEEHQKLLPSTIFLSTAFMPLIDVVARISTSGELPVGAITAMLGGPFFL
YLLMRRSV

Sequences:

>Translated_328_residues
MTNSFDFVKGKVVAALLALNLLALLLSLGVGSGGYTYLFQVITGNADETATKIVLSYRLPRYLMAVIAGASLSASGCAMQ
ALFRNPLAEPYVLGVASGASVGAALSVALGIETIYSRIGMAFFSALITAYVVYLIGSSSKFRDQTYAILLAGVAIASFFS
GLTSVLIYFSAQSMHKVIFWLMGSFSNPFVEEVLFALPITIAGLLYLLITSWNLNALLLGDEHAAAVGINVESFRKSLIA
VVALLTSTTVAISGVIGFVGIIVPHTMRILVGEEHQKLLPSTIFLSTAFMPLIDVVARISTSGELPVGAITAMLGGPFFL
YLLMRRSV
>Mature_327_residues
TNSFDFVKGKVVAALLALNLLALLLSLGVGSGGYTYLFQVITGNADETATKIVLSYRLPRYLMAVIAGASLSASGCAMQA
LFRNPLAEPYVLGVASGASVGAALSVALGIETIYSRIGMAFFSALITAYVVYLIGSSSKFRDQTYAILLAGVAIASFFSG
LTSVLIYFSAQSMHKVIFWLMGSFSNPFVEEVLFALPITIAGLLYLLITSWNLNALLLGDEHAAAVGINVESFRKSLIAV
VALLTSTTVAISGVIGFVGIIVPHTMRILVGEEHQKLLPSTIFLSTAFMPLIDVVARISTSGELPVGAITAMLGGPFFLY
LLMRRSV

Specific function: Part of the binding-protein-dependent transport system for hemin; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0609

COG function: function code P; ABC-type Fe3+-siderophore transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. FecCD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788004, Length=276, Percent_Identity=35.5072463768116, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1786805, Length=297, Percent_Identity=35.3535353535354, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1790741, Length=320, Percent_Identity=31.5625, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1790740, Length=308, Percent_Identity=33.1168831168831, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1786804, Length=304, Percent_Identity=26.9736842105263, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1786347, Length=309, Percent_Identity=28.1553398058252, Blast_Score=86, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000522 [H]

Pfam domain/function: PF01032 FecCD [H]

EC number: NA

Molecular weight: Translated: 34744; Mature: 34612

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNSFDFVKGKVVAALLALNLLALLLSLGVGSGGYTYLFQVITGNADETATKIVLSYRLP
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHEEEEEHCCCCCHHHHHHHHHHHHH
RYLMAVIAGASLSASGCAMQALFRNPLAEPYVLGVASGASVGAALSVALGIETIYSRIGM
HHHHHHHHCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
AFFSALITAYVVYLIGSSSKFRDQTYAILLAGVAIASFFSGLTSVLIYFSAQSMHKVIFW
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LMGSFSNPFVEEVLFALPITIAGLLYLLITSWNLNALLLGDEHAAAVGINVESFRKSLIA
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEECCHHHHHHHHHH
VVALLTSTTVAISGVIGFVGIIVPHTMRILVGEEHQKLLPSTIFLSTAFMPLIDVVARIS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
TSGELPVGAITAMLGGPFFLYLLMRRSV
CCCCCCHHHHHHHHCCHHHHHHHHHHCC
>Mature Secondary Structure 
TNSFDFVKGKVVAALLALNLLALLLSLGVGSGGYTYLFQVITGNADETATKIVLSYRLP
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHEEEEEHCCCCCHHHHHHHHHHHHH
RYLMAVIAGASLSASGCAMQALFRNPLAEPYVLGVASGASVGAALSVALGIETIYSRIGM
HHHHHHHHCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
AFFSALITAYVVYLIGSSSKFRDQTYAILLAGVAIASFFSGLTSVLIYFSAQSMHKVIFW
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LMGSFSNPFVEEVLFALPITIAGLLYLLITSWNLNALLLGDEHAAAVGINVESFRKSLIA
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEECCHHHHHHHHHH
VVALLTSTTVAISGVIGFVGIIVPHTMRILVGEEHQKLLPSTIFLSTAFMPLIDVVARIS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
TSGELPVGAITAMLGGPFFLYLLMRRSV
CCCCCCHHHHHHHHCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; cob(I)alamin [Periplasm]; H2O [C]

Specific reaction: ATP + cob(I)alamin [Periplasm] + H2O = ADP + phosphate + cob(I)alamin [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9026634; 11586360; 12142430 [H]