Click here to switch to the map view.

The map label for this gene is gcp

Identifier: 11498712

GI number: 11498712

Start: 995286

End: 996257

Strand: Reverse

Name: gcp

Synonym: AF1112

Alternate gene names: 11498712

Gene position: 996257-995286 (Counterclockwise)

Preceding gene: 11498713

Following gene: 11498710

Centisome position: 45.73

GC content: 52.57

Gene sequence:

>972_bases
ATGATTGCTCTCGGAATAGAGGGCACGGCCTGGAGTTTGAGCATAGGTGTTGTTGATGAGGAGGGCGTTATAGCCCTCGA
AAATGACCCCTACATCCCGAAAGAGGGAGGGATACACCCGAGAGAGGCTTCACAGCATCACAGCGAAAGGCTCCCCTCCC
TGCTTTCAAGAGTTTTTGAGAAAGTCGATAAGAATTCCATCGACGTCGTTGCCTTTTCTCAGGGCCCCGGAATGGGGCCT
TGTCTCAGAGTTGTGGCGACCGCAGCCAGACTTCTGGCCATTAAGCTCGAAAAACCCCTTGTCGGTGTAAACCACTGCCT
CGCCCACGTTGAGGTAGGGAGGTGGCAAACTGGTGCAAGAAAGCCGGTTTCTCTCTACGTGAGCGGCGGAAACAGTCAGG
TCATTGCAAGGAGGGGGAACAGGTACAGAGTTTTCGGCGAGACGCTCGACATAGGTATAGGCAACGCCTTGGACAAATTG
GCGAGGCACATGGGACTCAAACATCCCGGTGGGCCTAAGATAGAGGAGCTTGCAAAGAAGGGGCAAAAATACCACTTTCT
GCCATACGTGGTAAAGGGGATGGACTTCTCCTTCAGCGGTATGGTTACTGCAGCACAGCGCCTTTTTGATTCAGGAGTTA
GAATGGAGGACGTTGCATTCAGCTTCCAGGAGACTGCCTTCGCAATGCTGACGGAGGTGACGGAAAGAGCCCTCGCCTAT
CTTGATCTGAACGAGGTGCTGCTTGTTGGTGGTGTAGCTGCAAACAAAAGGCTGCAGGAGATGCTCAGAATTATGTGCGA
GGACAGGGGAGCAAAATTTTATGTGCCGCCAAAAGAGCTCGCCGGAGACAACGGGGCGATGATTGCCTATACCGGCCTGC
TGATGTACAAGCACGGTCACCAGACTCCCGTAGAGAAATCCTACGTCAGGCCCGATTTCAGAATAGAGGATGTTGAGGTA
AATTGGGATTAA

Upstream 100 bases:

>100_bases
AGAAGAAATTCTGTCCGAGATGTGGTGAGGGAGTCTTCTTAGCTGAGCACAAGGACAGGCTAAGCTGCGGCAAGTGCGGC
TACACTGAGTTCAAGAAGAA

Downstream 100 bases:

>100_bases
TCCTTCCCTTCAATCTCCTTCTTCATCAGCTTCTGCCTCTCCTTTGCAGTGTAACCGGTAAGCTCTTCGAGAAACTTGTT
GTAGGTCTTAAGCGTTTTTA

Product: putative DNA-binding/iron metalloprotein/AP endonuclease

Products: NA

Alternate protein names: Glycoprotease

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MIALGIEGTAWSLSIGVVDEEGVIALENDPYIPKEGGIHPREASQHHSERLPSLLSRVFEKVDKNSIDVVAFSQGPGMGP
CLRVVATAARLLAIKLEKPLVGVNHCLAHVEVGRWQTGARKPVSLYVSGGNSQVIARRGNRYRVFGETLDIGIGNALDKL
ARHMGLKHPGGPKIEELAKKGQKYHFLPYVVKGMDFSFSGMVTAAQRLFDSGVRMEDVAFSFQETAFAMLTEVTERALAY
LDLNEVLLVGGVAANKRLQEMLRIMCEDRGAKFYVPPKELAGDNGAMIAYTGLLMYKHGHQTPVEKSYVRPDFRIEDVEV
NWD

Sequences:

>Translated_323_residues
MIALGIEGTAWSLSIGVVDEEGVIALENDPYIPKEGGIHPREASQHHSERLPSLLSRVFEKVDKNSIDVVAFSQGPGMGP
CLRVVATAARLLAIKLEKPLVGVNHCLAHVEVGRWQTGARKPVSLYVSGGNSQVIARRGNRYRVFGETLDIGIGNALDKL
ARHMGLKHPGGPKIEELAKKGQKYHFLPYVVKGMDFSFSGMVTAAQRLFDSGVRMEDVAFSFQETAFAMLTEVTERALAY
LDLNEVLLVGGVAANKRLQEMLRIMCEDRGAKFYVPPKELAGDNGAMIAYTGLLMYKHGHQTPVEKSYVRPDFRIEDVEV
NWD
>Mature_323_residues
MIALGIEGTAWSLSIGVVDEEGVIALENDPYIPKEGGIHPREASQHHSERLPSLLSRVFEKVDKNSIDVVAFSQGPGMGP
CLRVVATAARLLAIKLEKPLVGVNHCLAHVEVGRWQTGARKPVSLYVSGGNSQVIARRGNRYRVFGETLDIGIGNALDKL
ARHMGLKHPGGPKIEELAKKGQKYHFLPYVVKGMDFSFSGMVTAAQRLFDSGVRMEDVAFSFQETAFAMLTEVTERALAY
LDLNEVLLVGGVAANKRLQEMLRIMCEDRGAKFYVPPKELAGDNGAMIAYTGLLMYKHGHQTPVEKSYVRPDFRIEDVEV
NWD

Specific function: Could Be A Metalloprotease. [C]

COG id: COG0533

COG function: function code O; Metal-dependent proteases with possible chaperone activity

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M22 family

Homologues:

Organism=Homo sapiens, GI8923380, Length=329, Percent_Identity=45.8966565349544, Blast_Score=289, Evalue=3e-78,
Organism=Homo sapiens, GI116812636, Length=331, Percent_Identity=30.2114803625378, Blast_Score=120, Evalue=2e-27,
Organism=Escherichia coli, GI1789445, Length=337, Percent_Identity=33.5311572700297, Blast_Score=147, Evalue=9e-37,
Organism=Caenorhabditis elegans, GI71995670, Length=331, Percent_Identity=43.202416918429, Blast_Score=267, Evalue=5e-72,
Organism=Caenorhabditis elegans, GI17557464, Length=325, Percent_Identity=26.4615384615385, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6322891, Length=368, Percent_Identity=40.7608695652174, Blast_Score=251, Evalue=1e-67,
Organism=Saccharomyces cerevisiae, GI6320099, Length=306, Percent_Identity=29.4117647058824, Blast_Score=116, Evalue=4e-27,
Organism=Drosophila melanogaster, GI21357207, Length=343, Percent_Identity=45.1895043731778, Blast_Score=299, Evalue=2e-81,
Organism=Drosophila melanogaster, GI20129063, Length=334, Percent_Identity=29.0419161676647, Blast_Score=95, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCP_ARCFU (O29153)

Other databases:

- EMBL:   AE000782
- PIR:   G69388
- RefSeq:   NP_069941.1
- ProteinModelPortal:   O29153
- SMR:   O29153
- MEROPS:   M22.950
- GeneID:   1484334
- GenomeReviews:   AE000782_GR
- KEGG:   afu:AF1112
- NMPDR:   fig|224325.1.peg.1100
- TIGR:   AF_1112
- HOGENOM:   HBG304663
- OMA:   TQRFRTD
- ProtClustDB:   PRK14878
- BioCyc:   AFUL224325:AF_1112-MONOMER
- BRENDA:   3.4.24.57
- GO:   GO:0006508
- HAMAP:   MF_01446
- InterPro:   IPR022449
- InterPro:   IPR000905
- InterPro:   IPR017861
- PANTHER:   PTHR11735
- PRINTS:   PR00789
- TIGRFAMs:   TIGR03722
- TIGRFAMs:   TIGR00329

Pfam domain/function: PF00814 Peptidase_M22

EC number: =3.4.24.57

Molecular weight: Translated: 35605; Mature: 35605

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: PS01016 GLYCOPROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIALGIEGTAWSLSIGVVDEEGVIALENDPYIPKEGGIHPREASQHHSERLPSLLSRVFE
CEEEECCCCEEEEEEEEECCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
KVDKNSIDVVAFSQGPGMGPCLRVVATAARLLAIKLEKPLVGVNHCLAHVEVGRWQTGAR
HHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHCCCCCCCCCC
KPVSLYVSGGNSQVIARRGNRYRVFGETLDIGIGNALDKLARHMGLKHPGGPKIEELAKK
CCEEEEEECCCCEEEEECCCEEEEECCEEECCCCHHHHHHHHHCCCCCCCCCCHHHHHHC
GQKYHFLPYVVKGMDFSFSGMVTAAQRLFDSGVRMEDVAFSFQETAFAMLTEVTERALAY
CCCEEECHHHEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LDLNEVLLVGGVAANKRLQEMLRIMCEDRGAKFYVPPKELAGDNGAMIAYTGLLMYKHGH
CCCCCEEEECCCHHHHHHHHHHHHHHHCCCCEEEECHHHHCCCCCCEEEEEEEEEEECCC
QTPVEKSYVRPDFRIEDVEVNWD
CCCCHHHHCCCCCEEEEEEECCC
>Mature Secondary Structure
MIALGIEGTAWSLSIGVVDEEGVIALENDPYIPKEGGIHPREASQHHSERLPSLLSRVFE
CEEEECCCCEEEEEEEEECCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
KVDKNSIDVVAFSQGPGMGPCLRVVATAARLLAIKLEKPLVGVNHCLAHVEVGRWQTGAR
HHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHCCCCCCCCCC
KPVSLYVSGGNSQVIARRGNRYRVFGETLDIGIGNALDKLARHMGLKHPGGPKIEELAKK
CCEEEEEECCCCEEEEECCCEEEEECCEEECCCCHHHHHHHHHCCCCCCCCCCHHHHHHC
GQKYHFLPYVVKGMDFSFSGMVTAAQRLFDSGVRMEDVAFSFQETAFAMLTEVTERALAY
CCCEEECHHHEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LDLNEVLLVGGVAANKRLQEMLRIMCEDRGAKFYVPPKELAGDNGAMIAYTGLLMYKHGH
CCCCCEEEECCCHHHHHHHHHHHHHHHCCCCEEEECHHHHCCCCCCEEEEEEEEEEECCC
QTPVEKSYVRPDFRIEDVEVNWD
CCCCHHHHCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475