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The map label for this gene is ilvD

Identifier: 11498619

GI number: 11498619

Start: 914249

End: 915892

Strand: Reverse

Name: ilvD

Synonym: AF1014

Alternate gene names: 11498619

Gene position: 915892-914249 (Counterclockwise)

Preceding gene: 11498622

Following gene: 11498618

Centisome position: 42.04

GC content: 54.26

Gene sequence:

>1644_bases
ATGAGAAGTGATGAGGTTAAGAAAGGCATCGACAGAGTCGCTCACAGGGCTCTTCTGAAGGCCCTCGGCGTTACTGACGA
TGAGATGGATAAGCCCTTCATTGGAGTTGCCAACGCCTACAACACCATCGTTCCCGGGCACATGACTCTCGACAAGCTCA
CTCAGGCTGTTAAGGAGGGGGTTTATGCTGCGGGAGGTGTGCCCTTCGAGTTCGGCATAATAGGCATCTGTGACGGCATT
GCCATGGGGCATGAGGGGATGTGCTTCTCCCTACCATCGAGAGAGCTTGTTGCGGACACAATTGAGGCGATGGTTGAGGC
TCACCGCTTTGATGGGTTAGTTGTGGTTGCGAGCTGCGACAAAATCATTCCGGGAATGCTCATGGCGATGCTCCGCCTCA
ACATCCCCGCAATTGCCGTCACAGGTGGCCCAATGCCCTACGAGAGAGTAGGGGGAGAGAAAGTCTCCATAAAAGATGCC
TTTGAGGCAGCGGGGATGTACAAGGCGGGAAAGCTCGATGACGCTGGCTTAAAGCTCTACGAGGACTACTGCGCCCCCTA
CTGCGGAAGCTGTCAGGGGCTTTACACGGCCAACAGTATGCAGATTCTCACCGAAACTCTTGGCCTAAGCCTGCCCTACT
GCTCCACCTCTCCCTGCCCATCGTCGAGAAAGCTCAGAATAGCGAAGCAGAGCGGGAAGAGGGTAGTTGAGCTTGTTATG
CAGAACATCAAACCCCTCGACTTCGTTAACGAGCGCTCCTTTGAGAACGCCATCACCATGGACATGCTGGTTGGGGGTTC
AACCAACACAGTTCTCCATTTGCCCGCAATAGCCAAAGAGGCGGGAATAAGGCTGAGCCTCGACCTCTTCGACGAGATAA
GCAGGAGAACTCCCCACATAGTCTCCATCGACCCTGCGAGCAAGGAGATGGTTGTTGATTTAGATGAGAGCGGTGGGGTG
CCGATGCTGATAAAGAAGGCAAGGAAGTACTTCCACGATGAAATGACAGTGAGCGGAAAAACGCTCTACGAGATTGCGGA
GATGGCCGTGCTGAGAGGGAGGGACATTATCGCCTCACCCGACAACCCGCTGCACAAAGAGGGAGGAATAGCCATTCTGA
AAGGCAATCTGGCCGAAAATGGTGCCGTAATCAAGGCTGCTGCAGTGAGCGAGGACATGATGAGGTTTGAGGGAACTGCA
AAGGTTTACGACAGCGAGAAGGAGGCTCTGAACGCCATACTTGATGGAAAGGTTGAGGAAGGGGATGTGGTGGTTATCAG
GTATATGGGGCCTAAAGGAGCTCCGGGAATGCCTGAGATGCTCTTGCCAACAGCAGCAATCTCAGGGCTGGGATTGCAGA
AGGTGGCTTTGATCACAGACGGAAGGTTTAGTGGAGCGACGAGGGGGCCGTGCATCGGCCACGTGTCGCCTGAGGCTGCT
GTCGGTGGAAACATAGCTCTGGTTGAGGATGGAGATAAAATTTCCATAGACATCCCCGCAAGAAAGCTGGAAGTGAAGCT
CAGCGATGAGGAGCTGGCAGAGAGGAGGGCCAAGTGGAAGCCTAAGGAGAAGGAGCTGAAGGGCTACTTGGCAAAGTATG
CAAAGCTCGTGAGGGGGGCTGAGGAAGGTGCCGCCCTTCTTTGA

Upstream 100 bases:

>100_bases
ATAAATGTTTTGTTTTTTGTCAAAATCTTCCCGATGCTTTCTTATTGTTCTTCACACCATCCAACCAAAACTTAAAAAAT
AATGAAGCACTTCTAAAACC

Downstream 100 bases:

>100_bases
TTTTTTAATGTCAGCAGGCATCGCCTTCTTTGTTTTTATCATTTCGGCAATAATACTGCCGGGCTTCTTCATCTGGATTG
GGCTGAAGGTTGTGGGGAAG

Product: dihydroxy-acid dehydratase (ilvD)

Products: NA

Alternate protein names: DAD

Number of amino acids: Translated: 547; Mature: 547

Protein sequence:

>547_residues
MRSDEVKKGIDRVAHRALLKALGVTDDEMDKPFIGVANAYNTIVPGHMTLDKLTQAVKEGVYAAGGVPFEFGIIGICDGI
AMGHEGMCFSLPSRELVADTIEAMVEAHRFDGLVVVASCDKIIPGMLMAMLRLNIPAIAVTGGPMPYERVGGEKVSIKDA
FEAAGMYKAGKLDDAGLKLYEDYCAPYCGSCQGLYTANSMQILTETLGLSLPYCSTSPCPSSRKLRIAKQSGKRVVELVM
QNIKPLDFVNERSFENAITMDMLVGGSTNTVLHLPAIAKEAGIRLSLDLFDEISRRTPHIVSIDPASKEMVVDLDESGGV
PMLIKKARKYFHDEMTVSGKTLYEIAEMAVLRGRDIIASPDNPLHKEGGIAILKGNLAENGAVIKAAAVSEDMMRFEGTA
KVYDSEKEALNAILDGKVEEGDVVVIRYMGPKGAPGMPEMLLPTAAISGLGLQKVALITDGRFSGATRGPCIGHVSPEAA
VGGNIALVEDGDKISIDIPARKLEVKLSDEELAERRAKWKPKEKELKGYLAKYAKLVRGAEEGAALL

Sequences:

>Translated_547_residues
MRSDEVKKGIDRVAHRALLKALGVTDDEMDKPFIGVANAYNTIVPGHMTLDKLTQAVKEGVYAAGGVPFEFGIIGICDGI
AMGHEGMCFSLPSRELVADTIEAMVEAHRFDGLVVVASCDKIIPGMLMAMLRLNIPAIAVTGGPMPYERVGGEKVSIKDA
FEAAGMYKAGKLDDAGLKLYEDYCAPYCGSCQGLYTANSMQILTETLGLSLPYCSTSPCPSSRKLRIAKQSGKRVVELVM
QNIKPLDFVNERSFENAITMDMLVGGSTNTVLHLPAIAKEAGIRLSLDLFDEISRRTPHIVSIDPASKEMVVDLDESGGV
PMLIKKARKYFHDEMTVSGKTLYEIAEMAVLRGRDIIASPDNPLHKEGGIAILKGNLAENGAVIKAAAVSEDMMRFEGTA
KVYDSEKEALNAILDGKVEEGDVVVIRYMGPKGAPGMPEMLLPTAAISGLGLQKVALITDGRFSGATRGPCIGHVSPEAA
VGGNIALVEDGDKISIDIPARKLEVKLSDEELAERRAKWKPKEKELKGYLAKYAKLVRGAEEGAALL
>Mature_547_residues
MRSDEVKKGIDRVAHRALLKALGVTDDEMDKPFIGVANAYNTIVPGHMTLDKLTQAVKEGVYAAGGVPFEFGIIGICDGI
AMGHEGMCFSLPSRELVADTIEAMVEAHRFDGLVVVASCDKIIPGMLMAMLRLNIPAIAVTGGPMPYERVGGEKVSIKDA
FEAAGMYKAGKLDDAGLKLYEDYCAPYCGSCQGLYTANSMQILTETLGLSLPYCSTSPCPSSRKLRIAKQSGKRVVELVM
QNIKPLDFVNERSFENAITMDMLVGGSTNTVLHLPAIAKEAGIRLSLDLFDEISRRTPHIVSIDPASKEMVVDLDESGGV
PMLIKKARKYFHDEMTVSGKTLYEIAEMAVLRGRDIIASPDNPLHKEGGIAILKGNLAENGAVIKAAAVSEDMMRFEGTA
KVYDSEKEALNAILDGKVEEGDVVVIRYMGPKGAPGMPEMLLPTAAISGLGLQKVALITDGRFSGATRGPCIGHVSPEAA
VGGNIALVEDGDKISIDIPARKLEVKLSDEELAERRAKWKPKEKELKGYLAKYAKLVRGAEEGAALL

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family

Homologues:

Organism=Escherichia coli, GI48994964, Length=603, Percent_Identity=42.620232172471, Blast_Score=432, Evalue=1e-122,
Organism=Escherichia coli, GI1788157, Length=509, Percent_Identity=31.8271119842829, Blast_Score=217, Evalue=1e-57,
Organism=Escherichia coli, GI2367371, Length=512, Percent_Identity=33.7890625, Blast_Score=215, Evalue=7e-57,
Organism=Escherichia coli, GI1786464, Length=497, Percent_Identity=32.9979879275654, Blast_Score=207, Evalue=1e-54,
Organism=Saccharomyces cerevisiae, GI6322476, Length=548, Percent_Identity=40.6934306569343, Blast_Score=369, Evalue=1e-103,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ILVD_ARCFU (O29248)

Other databases:

- EMBL:   AE000782
- PIR:   F69376
- RefSeq:   NP_069847.1
- GeneID:   1484237
- GenomeReviews:   AE000782_GR
- KEGG:   afu:AF1014
- NMPDR:   fig|224325.1.peg.1006
- TIGR:   AF_1014
- HOGENOM:   HBG671001
- OMA:   AKETPQL
- ProtClustDB:   CLSK2746713
- BioCyc:   AFUL224325:AF_1014-MONOMER
- BRENDA:   4.2.1.9
- HAMAP:   MF_00012
- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558
- PANTHER:   PTHR21000
- TIGRFAMs:   TIGR00110

Pfam domain/function: PF00920 ILVD_EDD; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.9

Molecular weight: Translated: 58743; Mature: 58743

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS00886 ILVD_EDD_1; PS00887 ILVD_EDD_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSDEVKKGIDRVAHRALLKALGVTDDEMDKPFIGVANAYNTIVPGHMTLDKLTQAVKEG
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHCC
VYAAGGVPFEFGIIGICDGIAMGHEGMCFSLPSRELVADTIEAMVEAHRFDGLVVVASCD
CEECCCCCEECCEEEEECCHHCCCCCCEEECCCHHHHHHHHHHHHHHHHCCCEEEEECCC
KIIPGMLMAMLRLNIPAIAVTGGPMPYERVGGEKVSIKDAFEAAGMYKAGKLDDAGLKLY
HHHHHHHHHHHHCCCCEEEEECCCCCHHHCCCCEEEHHHHHHHCCCCCCCCCCCCHHHHH
EDYCAPYCGSCQGLYTANSMQILTETLGLSLPYCSTSPCPSSRKLRIAKQSGKRVVELVM
HHHCCCCCCCCCCEEECCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEHHCCHHHHHHHH
QNIKPLDFVNERSFENAITMDMLVGGSTNTVLHLPAIAKEAGIRLSLDLFDEISRRTPHI
HCCCCCCHHCCCCCCCCEEEEEEECCCCCCEEECCHHHHHCCCEEEHHHHHHHHHCCCCE
VSIDPASKEMVVDLDESGGVPMLIKKARKYFHDEMTVSGKTLYEIAEMAVLRGRDIIASP
EEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCEECCHHHHHHHHHHHHCCCCEEECC
DNPLHKEGGIAILKGNLAENGAVIKAAAVSEDMMRFEGTAKVYDSEKEALNAILDGKVEE
CCCCCCCCCEEEEECCCCCCCCEEEEHHHHHHHHHHCCCHHCCCCHHHHHHHHHCCCCCC
GDVVVIRYMGPKGAPGMPEMLLPTAAISGLGLQKVALITDGRFSGATRGPCIGHVSPEAA
CCEEEEEEECCCCCCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCEEECCCCCHH
VGGNIALVEDGDKISIDIPARKLEVKLSDEELAERRAKWKPKEKELKGYLAKYAKLVRGA
CCCCEEEEECCCEEEEECCCEEEEEEECHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
EEGAALL
CCCCCCC
>Mature Secondary Structure
MRSDEVKKGIDRVAHRALLKALGVTDDEMDKPFIGVANAYNTIVPGHMTLDKLTQAVKEG
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHCC
VYAAGGVPFEFGIIGICDGIAMGHEGMCFSLPSRELVADTIEAMVEAHRFDGLVVVASCD
CEECCCCCEECCEEEEECCHHCCCCCCEEECCCHHHHHHHHHHHHHHHHCCCEEEEECCC
KIIPGMLMAMLRLNIPAIAVTGGPMPYERVGGEKVSIKDAFEAAGMYKAGKLDDAGLKLY
HHHHHHHHHHHHCCCCEEEEECCCCCHHHCCCCEEEHHHHHHHCCCCCCCCCCCCHHHHH
EDYCAPYCGSCQGLYTANSMQILTETLGLSLPYCSTSPCPSSRKLRIAKQSGKRVVELVM
HHHCCCCCCCCCCEEECCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEHHCCHHHHHHHH
QNIKPLDFVNERSFENAITMDMLVGGSTNTVLHLPAIAKEAGIRLSLDLFDEISRRTPHI
HCCCCCCHHCCCCCCCCEEEEEEECCCCCCEEECCHHHHHCCCEEEHHHHHHHHHCCCCE
VSIDPASKEMVVDLDESGGVPMLIKKARKYFHDEMTVSGKTLYEIAEMAVLRGRDIIASP
EEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCEECCHHHHHHHHHHHHCCCCEEECC
DNPLHKEGGIAILKGNLAENGAVIKAAAVSEDMMRFEGTAKVYDSEKEALNAILDGKVEE
CCCCCCCCCEEEEECCCCCCCCEEEEHHHHHHHHHHCCCHHCCCCHHHHHHHHHCCCCCC
GDVVVIRYMGPKGAPGMPEMLLPTAAISGLGLQKVALITDGRFSGATRGPCIGHVSPEAA
CCEEEEEEECCCCCCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCEEECCCCCHH
VGGNIALVEDGDKISIDIPARKLEVKLSDEELAERRAKWKPKEKELKGYLAKYAKLVRGA
CCCCEEEEECCCEEEEECCCEEEEEEECHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
EEGAALL
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475