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The map label for this gene is nadE

Identifier: 11498605

GI number: 11498605

Start: 902708

End: 903451

Strand: Reverse

Name: nadE

Synonym: AF1000

Alternate gene names: 11498605

Gene position: 903451-902708 (Counterclockwise)

Preceding gene: 11498606

Following gene: 11498604

Centisome position: 41.47

GC content: 49.87

Gene sequence:

>744_bases
ATGAACTTTGAGAAGGTCGTTGAGAGAATTTGTGATTTCATAAGGGGTGTTGTTTCATCATCAGGTTCAACGGGAGTTGT
TCTGGGATTGAGTGGTGGTGTCGACAGCGCAACCGTTGCTTATCTCTGCGTGAGGGCTCTTGGGAGCGAGAGAGTTTTTG
CGCTCATCATGCCTGAGACTGGAGTGACTCCTGAGCAGGATGTTGAGGATGCGATAAATGTCGCGGAGAGCCTCGGAATG
GAATACAAGCTGATTGAAATCAATGACATCGTTAGAGTTTTCAAAGAAAAGGCCGGAGAGGGAAGCAAGATCGCGGAGGC
GAATTTAAAGCCGAGGATAAGAATGGTGCTCAACTACTATCATGCCAACAGCATGAACAGGCTTGTTGCGGGGACCGGAA
ACAAAAGCGAGCTAATGGTGGGGTACTTTACCAAATACGGGGATGGCGGAGTGGATTTCCTGCCAATAGGAGATCTCTAC
AAGACCGAGGTATTTCAGCTTGCCGCCTATCTCGGAGTTCCAAGGAGAATAATCGAGAAGAAGCCTTCTGCAAGGCTGTG
GCCTGGCCAGACCGATGAGGAGGAGATGGGAATAAGCTACGCCGAGCTTGACGAAATCTTAAAGCTGATCGAGAAGGGGG
AGAGGAGAGACGATGAGAAGTTCAGGAGAGTTGTGCAGATGGTTGAGAGGTCGAGGCACAAGAGGGAGATGCCTCCCGTA
GCAAGGGTGAGAGATTTGCTATGA

Upstream 100 bases:

>100_bases
CCAGCCGGATGGGAAGGTTTGCCAGTTCTCCTACGGAAGCTAACCCCTTTATTTTTTAACTGGTAAAAAGATTTTAATCC
CCACTCCCCTACAGCTTTCC

Downstream 100 bases:

>100_bases
AAGGAGCTTTGCTTTTAGTTCTTCTGATACTCACGATTTCAACGACCTCCGCTGTAGAAATTTACGGGAAGGTTTACAGG
TGGGACACGCTTGACGTTTT

Product: NAD synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 247; Mature: 247

Protein sequence:

>247_residues
MNFEKVVERICDFIRGVVSSSGSTGVVLGLSGGVDSATVAYLCVRALGSERVFALIMPETGVTPEQDVEDAINVAESLGM
EYKLIEINDIVRVFKEKAGEGSKIAEANLKPRIRMVLNYYHANSMNRLVAGTGNKSELMVGYFTKYGDGGVDFLPIGDLY
KTEVFQLAAYLGVPRRIIEKKPSARLWPGQTDEEEMGISYAELDEILKLIEKGERRDDEKFRRVVQMVERSRHKREMPPV
ARVRDLL

Sequences:

>Translated_247_residues
MNFEKVVERICDFIRGVVSSSGSTGVVLGLSGGVDSATVAYLCVRALGSERVFALIMPETGVTPEQDVEDAINVAESLGM
EYKLIEINDIVRVFKEKAGEGSKIAEANLKPRIRMVLNYYHANSMNRLVAGTGNKSELMVGYFTKYGDGGVDFLPIGDLY
KTEVFQLAAYLGVPRRIIEKKPSARLWPGQTDEEEMGISYAELDEILKLIEKGERRDDEKFRRVVQMVERSRHKREMPPV
ARVRDLL
>Mature_247_residues
MNFEKVVERICDFIRGVVSSSGSTGVVLGLSGGVDSATVAYLCVRALGSERVFALIMPETGVTPEQDVEDAINVAESLGM
EYKLIEINDIVRVFKEKAGEGSKIAEANLKPRIRMVLNYYHANSMNRLVAGTGNKSELMVGYFTKYGDGGVDFLPIGDLY
KTEVFQLAAYLGVPRRIIEKKPSARLWPGQTDEEEMGISYAELDEILKLIEKGERRDDEKFRRVVQMVERSRHKREMPPV
ARVRDLL

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family

Homologues:

Organism=Homo sapiens, GI41393551, Length=272, Percent_Identity=29.7794117647059, Blast_Score=72, Evalue=7e-13,
Organism=Escherichia coli, GI1788036, Length=263, Percent_Identity=29.6577946768061, Blast_Score=97, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17538756, Length=144, Percent_Identity=36.1111111111111, Blast_Score=67, Evalue=6e-12,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NADE_ARCFU (O29262)

Other databases:

- EMBL:   AE000782
- PIR:   H69374
- RefSeq:   NP_069833.1
- ProteinModelPortal:   O29262
- SMR:   O29262
- GeneID:   1484223
- GenomeReviews:   AE000782_GR
- KEGG:   afu:AF1000
- NMPDR:   fig|224325.1.peg.992
- TIGR:   AF_1000
- HOGENOM:   HBG351567
- OMA:   FRYARDW
- ProtClustDB:   PRK13980
- BioCyc:   AFUL224325:AF_1000-MONOMER
- BRENDA:   6.3.1.5
- HAMAP:   MF_00193
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- TIGRFAMs:   TIGR00552

Pfam domain/function: PF02540 NAD_synthase

EC number: =6.3.1.5

Molecular weight: Translated: 27653; Mature: 27653

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: NA

Important sites: ACT_SITE 31-31

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFEKVVERICDFIRGVVSSSGSTGVVLGLSGGVDSATVAYLCVRALGSERVFALIMPET
CCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCC
GVTPEQDVEDAINVAESLGMEYKLIEINDIVRVFKEKAGEGSKIAEANLKPRIRMVLNYY
CCCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCCCCHHCCCCHHHHHHHHHH
HANSMNRLVAGTGNKSELMVGYFTKYGDGGVDFLPIGDLYKTEVFQLAAYLGVPRRIIEK
HHCCCCCEEECCCCCCCEEEEEEECCCCCCCCEEECCHHHHHHHHHHHHHHCCCHHHHCC
KPSARLWPGQTDEEEMGISYAELDEILKLIEKGERRDDEKFRRVVQMVERSRHKREMPPV
CCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHH
ARVRDLL
HHHHHCC
>Mature Secondary Structure
MNFEKVVERICDFIRGVVSSSGSTGVVLGLSGGVDSATVAYLCVRALGSERVFALIMPET
CCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCC
GVTPEQDVEDAINVAESLGMEYKLIEINDIVRVFKEKAGEGSKIAEANLKPRIRMVLNYY
CCCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCCCCHHCCCCHHHHHHHHHH
HANSMNRLVAGTGNKSELMVGYFTKYGDGGVDFLPIGDLYKTEVFQLAAYLGVPRRIIEK
HHCCCCCEEECCCCCCCEEEEEEECCCCCCCCEEECCHHHHHHHHHHHHHHCCCHHHHCC
KPSARLWPGQTDEEEMGISYAELDEILKLIEKGERRDDEKFRRVVQMVERSRHKREMPPV
CCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHH
ARVRDLL
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475