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The map label for this gene is radA

Identifier: 11498598

GI number: 11498598

Start: 894676

End: 895689

Strand: Reverse

Name: radA

Synonym: AF0993

Alternate gene names: 11498598

Gene position: 895689-894676 (Counterclockwise)

Preceding gene: 11498600

Following gene: 11498596

Centisome position: 41.12

GC content: 49.21

Gene sequence:

>1014_bases
ATGAGTGAAGAGAGCAACGAAGAGACGAAGATTATTGAGCTTGAGGATATTCCCGGAGTCGGTCCTGAGACGGCAAGAAA
GCTTAGAGAGGCTGGATATTCAACAATTGAGGCTGTTGCCGTAGCATCACCCTCAGAACTCGCCAATGTGGGTGGAATCA
CTGAAGGTAACGCGGTAAAGATAATTCAGGCGGCGAGGAAATTGGCCAACATAGGTGGTTTTGAGAGCGGAGATAAGGTT
TTGGAGAGAAGAAGGTCTGTTAAGAAAATAACTACTGGGAGCAAAGACCTCGACGAGTTGCTTGGTGGTGGAGTTGAGAC
CCAAGCCATAACCGAGTTTTTTGGAGAGTTTGGTAGTGGTAAGACTCAAATCTGCCATCAGCTCGCTGTAAACGTTCAGC
TTCCTGAGGATGAGGGAGGGCTTGAAGGCTCGGTGATTATCATCGACACAGAGAACACATTCAGGCCTGAGAGGATAATC
CAGATGGCTGAGGCGAAGGGGCTTGATGGCAATGAGGTTCTGAAGAACATCTACGTTGCTCAGGCTTACAACTCCAACCA
CCAGATGCTTCTTGTTGATAACGCGAAGGAGCTGGCTGAGAAACTGAAAAAGGAGGGGAGGCCAGTAAGGCTAATAATCG
TCGATTCTCTCATGTCACACTTCAGGGCGGAGTATGTCGGCAGGGGAACGCTGGCAGACAGACAGCAGAAGCTCAACAGA
CACCTCCACGACCTGATGAAATTCGGTGAGCTATACAACGCTGCAATTGTTGTGACGAATCAGGTTATGGCGAGGCCTGA
CGTGCTTTTCGGCGACCCGACGAAGCCTGTGGGGGGACACATCGTAGCCCACACGGCAACTTTCAGAATTTACCTCAAGA
AGGGCAAGGATGACCTTAGAATTGCCAGGCTGATCGACTCTCCACATTTGCCTGAAGGGGAGGCGATTTTCAGGGTGACT
GAGAGAGGAATAGAGGATGCTGAAGAGAAAGACAAAAAGAAAAGGAAAAAATAA

Upstream 100 bases:

>100_bases
CAGCTTGATAACCCTCTCCACGTGTCCCTCATCGTGCGATGACACCCTTTACGTCTGCCGAGACCAAAAAAATTTAATGG
ATTGTGTATGAGGTAGGGTA

Downstream 100 bases:

>100_bases
ATAATTTTTTACTCCTCAACAACCTCCTCCACGACCTGCATCAGCTCTTCGCTGTCCTCTGCATCGCCGTACTGGTTGAT
TAGCTGAATTATCTCCCTCA

Product: DNA repair and recombination protein RadA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 336

Protein sequence:

>337_residues
MSEESNEETKIIELEDIPGVGPETARKLREAGYSTIEAVAVASPSELANVGGITEGNAVKIIQAARKLANIGGFESGDKV
LERRRSVKKITTGSKDLDELLGGGVETQAITEFFGEFGSGKTQICHQLAVNVQLPEDEGGLEGSVIIIDTENTFRPERII
QMAEAKGLDGNEVLKNIYVAQAYNSNHQMLLVDNAKELAEKLKKEGRPVRLIIVDSLMSHFRAEYVGRGTLADRQQKLNR
HLHDLMKFGELYNAAIVVTNQVMARPDVLFGDPTKPVGGHIVAHTATFRIYLKKGKDDLRIARLIDSPHLPEGEAIFRVT
ERGIEDAEEKDKKKRKK

Sequences:

>Translated_337_residues
MSEESNEETKIIELEDIPGVGPETARKLREAGYSTIEAVAVASPSELANVGGITEGNAVKIIQAARKLANIGGFESGDKV
LERRRSVKKITTGSKDLDELLGGGVETQAITEFFGEFGSGKTQICHQLAVNVQLPEDEGGLEGSVIIIDTENTFRPERII
QMAEAKGLDGNEVLKNIYVAQAYNSNHQMLLVDNAKELAEKLKKEGRPVRLIIVDSLMSHFRAEYVGRGTLADRQQKLNR
HLHDLMKFGELYNAAIVVTNQVMARPDVLFGDPTKPVGGHIVAHTATFRIYLKKGKDDLRIARLIDSPHLPEGEAIFRVT
ERGIEDAEEKDKKKRKK
>Mature_336_residues
SEESNEETKIIELEDIPGVGPETARKLREAGYSTIEAVAVASPSELANVGGITEGNAVKIIQAARKLANIGGFESGDKVL
ERRRSVKKITTGSKDLDELLGGGVETQAITEFFGEFGSGKTQICHQLAVNVQLPEDEGGLEGSVIIIDTENTFRPERIIQ
MAEAKGLDGNEVLKNIYVAQAYNSNHQMLLVDNAKELAEKLKKEGRPVRLIIVDSLMSHFRAEYVGRGTLADRQQKLNRH
LHDLMKFGELYNAAIVVTNQVMARPDVLFGDPTKPVGGHIVAHTATFRIYLKKGKDDLRIARLIDSPHLPEGEAIFRVTE
RGIEDAEEKDKKKRKK

Specific function: Involved in DNA repair and in homologous recombination. Binds and assemble on single-stranded DNA to form a nucleoprotein filament. Hydrolyzes ATP in a ssDNA-dependent manner and promotes DNA strand exchange between homologous DNA molecules

COG id: COG0468

COG function: function code L; RecA/RadA recombinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the eukaryotic recA-like protein family

Homologues:

Organism=Homo sapiens, GI19924133, Length=330, Percent_Identity=46.6666666666667, Blast_Score=288, Evalue=5e-78,
Organism=Homo sapiens, GI23238219, Length=321, Percent_Identity=46.1059190031153, Blast_Score=273, Evalue=1e-73,
Organism=Homo sapiens, GI256017143, Length=336, Percent_Identity=42.8571428571429, Blast_Score=254, Evalue=6e-68,
Organism=Homo sapiens, GI256017141, Length=336, Percent_Identity=42.8571428571429, Blast_Score=254, Evalue=6e-68,
Organism=Homo sapiens, GI256017145, Length=248, Percent_Identity=47.1774193548387, Blast_Score=207, Evalue=9e-54,
Organism=Homo sapiens, GI17402896, Length=339, Percent_Identity=29.7935103244838, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI19924117, Length=334, Percent_Identity=24.5508982035928, Blast_Score=98, Evalue=9e-21,
Organism=Homo sapiens, GI46255039, Length=334, Percent_Identity=24.5508982035928, Blast_Score=98, Evalue=9e-21,
Organism=Homo sapiens, GI10835029, Length=334, Percent_Identity=24.5508982035928, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI153946430, Length=264, Percent_Identity=25.7575757575758, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI4885659, Length=264, Percent_Identity=25.7575757575758, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI153946427, Length=264, Percent_Identity=25.7575757575758, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI217416415, Length=181, Percent_Identity=31.4917127071823, Blast_Score=65, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI71997301, Length=307, Percent_Identity=48.5342019543974, Blast_Score=286, Evalue=8e-78,
Organism=Caenorhabditis elegans, GI71997295, Length=307, Percent_Identity=48.5342019543974, Blast_Score=286, Evalue=1e-77,
Organism=Saccharomyces cerevisiae, GI6321027, Length=315, Percent_Identity=45.0793650793651, Blast_Score=264, Evalue=1e-71,
Organism=Saccharomyces cerevisiae, GI6320942, Length=340, Percent_Identity=42.0588235294118, Blast_Score=251, Evalue=2e-67,
Organism=Saccharomyces cerevisiae, GI6320207, Length=201, Percent_Identity=32.8358208955224, Blast_Score=90, Evalue=5e-19,
Organism=Drosophila melanogaster, GI17864108, Length=304, Percent_Identity=45.7236842105263, Blast_Score=256, Evalue=1e-68,
Organism=Drosophila melanogaster, GI24651285, Length=282, Percent_Identity=46.4539007092199, Blast_Score=242, Evalue=3e-64,
Organism=Drosophila melanogaster, GI17136506, Length=277, Percent_Identity=30.3249097472924, Blast_Score=98, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24650551, Length=233, Percent_Identity=29.1845493562232, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: 1548 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800-1200 (L-broth) 40,000-60,000 (L-broth + Nalidixate) 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RADA_ARCFU (O29269)

Other databases:

- EMBL:   AE000782
- EMBL:   AF090198
- PIR:   A69374
- RefSeq:   NP_069826.1
- ProteinModelPortal:   O29269
- SMR:   O29269
- GeneID:   1484216
- GenomeReviews:   AE000782_GR
- KEGG:   afu:AF0993
- NMPDR:   fig|224325.1.peg.985
- TIGR:   AF_0993
- HOGENOM:   HBG413235
- OMA:   FVARAYN
- ProtClustDB:   PRK04301
- BioCyc:   AFUL224325:AF_0993-MONOMER
- HAMAP:   MF_00348
- InterPro:   IPR003593
- InterPro:   IPR013632
- InterPro:   IPR011938
- InterPro:   IPR016467
- InterPro:   IPR020588
- InterPro:   IPR010995
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR020587
- PIRSF:   PIRSF005856
- SMART:   SM00382
- SMART:   SM00278
- TIGRFAMs:   TIGR02236

Pfam domain/function: PF00633 HHH; PF08423 Rad51; SSF47794 Rad51_N

EC number: NA

Molecular weight: Translated: 37163; Mature: 37032

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS50162 RECA_2; PS50163 RECA_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEESNEETKIIELEDIPGVGPETARKLREAGYSTIEAVAVASPSELANVGGITEGNAVK
CCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCHHHHHHEECCCHHHHHCCCCCCCCHHH
IIQAARKLANIGGFESGDKVLERRRSVKKITTGSKDLDELLGGGVETQAITEFFGEFGSG
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHHHHCCC
KTQICHQLAVNVQLPEDEGGLEGSVIIIDTENTFRPERIIQMAEAKGLDGNEVLKNIYVA
HHHHHHHHHHEEECCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHH
QAYNSNHQMLLVDNAKELAEKLKKEGRPVRLIIVDSLMSHFRAEYVGRGTLADRQQKLNR
EEECCCCCEEEEECHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHH
HLHDLMKFGELYNAAIVVTNQVMARPDVLFGDPTKPVGGHIVAHTATFRIYLKKGKDDLR
HHHHHHHHHHHHCEEEEEECHHHCCCCCEECCCCCCCCCEEEEEEEEEEEEEECCCCHHH
IARLIDSPHLPEGEAIFRVTERGIEDAEEKDKKKRKK
HHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHCCC
>Mature Secondary Structure 
SEESNEETKIIELEDIPGVGPETARKLREAGYSTIEAVAVASPSELANVGGITEGNAVK
CCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCHHHHHHEECCCHHHHHCCCCCCCCHHH
IIQAARKLANIGGFESGDKVLERRRSVKKITTGSKDLDELLGGGVETQAITEFFGEFGSG
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHHHHCCC
KTQICHQLAVNVQLPEDEGGLEGSVIIIDTENTFRPERIIQMAEAKGLDGNEVLKNIYVA
HHHHHHHHHHEEECCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHH
QAYNSNHQMLLVDNAKELAEKLKKEGRPVRLIIVDSLMSHFRAEYVGRGTLADRQQKLNR
EEECCCCCEEEEECHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHH
HLHDLMKFGELYNAAIVVTNQVMARPDVLFGDPTKPVGGHIVAHTATFRIYLKKGKDDLR
HHHHHHHHHHHHCEEEEEECHHHCCCCCEECCCCCCCCCEEEEEEEEEEEEEECCCCHHH
IARLIDSPHLPEGEAIFRVTERGIEDAEEKDKKKRKK
HHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475; 9922255