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The map label for this gene is glnA

Identifier: 11498554

GI number: 11498554

Start: 849669

End: 851144

Strand: Reverse

Name: glnA

Synonym: AF0949

Alternate gene names: 11498554

Gene position: 851144-849669 (Counterclockwise)

Preceding gene: 11498560

Following gene: 11498553

Centisome position: 39.07

GC content: 52.57

Gene sequence:

>1476_bases
ATGGTAAGGCGGCTGAGAGGTGATTGTATGGAGGAGGTTGAGAGAGCTAAGGCGGTGTTGAAGGAAAATAACGTCAGACA
GGTACTCTGTGCATTTGCGGATGTTAGGGGATACCTTCAGATGTTCTCAATTCCCGCGAGGGAGTTTGTTGACGGCAGCG
CCTTCGAGAACGGAATCGGTTTTGACGGTTCCTCAGTCAGAGGGTTCAGAACGATAGAGAAGAGCGACATGGTCTGGATG
CCTGATGCATCATCGCTTAAAATCATTCCGTGGATAGATGACCCTATTCAGAAGTCCGCGATAATGTTCGGCAACGTTTA
CGAGGCGTGGGGCACTGAGATTGCTGACTGTGATCCAAGAGGTTACGTGGCCAAGAGGTACGAGGACATGCTGAAGAGCG
AGGGAATGTCAGCCATATTCGGGCCAGAAATTGAGTTCTTCCTTTTCGAGGGTGTTGACTTCACAAGGCTGAGCTGGGAC
ATGTGGGTCTCGCCCAACGGCGGTGCGGGAGACAGCTGGGGTCCGCCGAGAATCATGCCCATCAGCTCTGAACTTGAGAG
TGGGTACATGATAAGGCCCAAGGAGGGTTACTTCAGACCTCCACCTGAAGATACAACGGTTGAATATAGAAACGAGCTGG
TTTACTATTTGGAGCAGCTCGGAATAGACATTGAGTACCACCACCATGAGGTTGCCACAGCAGGGCAGGTGGAGCTGGAT
TTCAAGCCAAAACAGCTTGTGGATGTTGGTGATGCTTTCTACCTCTACAAGTTCGCAGCGAAGAACATTGCAGCGATGCA
CGGGTTGTATGCGACCTTCATGCCAAAGCCGCTTTACCTCGACAACGCGAGCGGAATGCACACCCACCAGAGCCTCTGGA
AGGGAGAGCCCTTCAGCGGCGAGGCTGTTTTTGCAGATCCTGATGATGAGTACATGCTGAGCCAGAAGGCCCGCTACTAC
ATAGGTGGGTTGCTGGAGCATGCAAAGGCTCTGACAGCACTCTGCGCTCCGACAGTGAACAGCTACAAGCGCCTTGTGCC
GGGCTTTGAAGCTCCCATCTACATCTGCTGGAGCCCGAGGAACAGGTCCGCTTTAGTGAGGGTGCCGATGTATGTCAAGA
AGCCCTCAGCAATAAGGGTGGAGTACAGGGGTGTTGACCCGAGCTGCAATCCTTATCTTGCCATCACAGCCCAGCTTGCT
GCCGGCTTAGACGGAATAAAGAAGAAGATAGACCCCGGTGACCCGCTGCTTGAGGACGTTTACGAGCTGACACCAGCACA
GAAGAGAGAGCTTGGAGTGGGAGAGCTGCCTACAACGCTTAGAGATGCGATAGACCATCTGGCAAGCGATGAGCTTATGC
AGGAAGTGCTGGGCAGCCACATCTTCGACGCCTTCATGGAGCTGAAGATTGACGAGTGGAACCAGTACTGTCTCTACATC
ACTCCGTGGGAATTCATGAAGTATTTTGATATCTGA

Upstream 100 bases:

>100_bases
GGTATGTATTTGAGAATTGGTGAAGATTTTTCAGATTTACCTACGCTTGCCTGCAGGTAATTTAACAAATCCCCTTGAAT
GCAAAAAAATATATATCGAA

Downstream 100 bases:

>100_bases
TTTCTTCAATTTGTTTTTCAGTTCATTTTTCAGGTTCTACCACTGATAATCAAAAACAAAATAAAGGAAATGTCAGAGAA
TGTATGTGGCTCCCGAAATC

Product: glutamine synthetase (glnA)

Products: NA

Alternate protein names: GS; Glutamate--ammonia ligase

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MVRRLRGDCMEEVERAKAVLKENNVRQVLCAFADVRGYLQMFSIPAREFVDGSAFENGIGFDGSSVRGFRTIEKSDMVWM
PDASSLKIIPWIDDPIQKSAIMFGNVYEAWGTEIADCDPRGYVAKRYEDMLKSEGMSAIFGPEIEFFLFEGVDFTRLSWD
MWVSPNGGAGDSWGPPRIMPISSELESGYMIRPKEGYFRPPPEDTTVEYRNELVYYLEQLGIDIEYHHHEVATAGQVELD
FKPKQLVDVGDAFYLYKFAAKNIAAMHGLYATFMPKPLYLDNASGMHTHQSLWKGEPFSGEAVFADPDDEYMLSQKARYY
IGGLLEHAKALTALCAPTVNSYKRLVPGFEAPIYICWSPRNRSALVRVPMYVKKPSAIRVEYRGVDPSCNPYLAITAQLA
AGLDGIKKKIDPGDPLLEDVYELTPAQKRELGVGELPTTLRDAIDHLASDELMQEVLGSHIFDAFMELKIDEWNQYCLYI
TPWEFMKYFDI

Sequences:

>Translated_491_residues
MVRRLRGDCMEEVERAKAVLKENNVRQVLCAFADVRGYLQMFSIPAREFVDGSAFENGIGFDGSSVRGFRTIEKSDMVWM
PDASSLKIIPWIDDPIQKSAIMFGNVYEAWGTEIADCDPRGYVAKRYEDMLKSEGMSAIFGPEIEFFLFEGVDFTRLSWD
MWVSPNGGAGDSWGPPRIMPISSELESGYMIRPKEGYFRPPPEDTTVEYRNELVYYLEQLGIDIEYHHHEVATAGQVELD
FKPKQLVDVGDAFYLYKFAAKNIAAMHGLYATFMPKPLYLDNASGMHTHQSLWKGEPFSGEAVFADPDDEYMLSQKARYY
IGGLLEHAKALTALCAPTVNSYKRLVPGFEAPIYICWSPRNRSALVRVPMYVKKPSAIRVEYRGVDPSCNPYLAITAQLA
AGLDGIKKKIDPGDPLLEDVYELTPAQKRELGVGELPTTLRDAIDHLASDELMQEVLGSHIFDAFMELKIDEWNQYCLYI
TPWEFMKYFDI
>Mature_491_residues
MVRRLRGDCMEEVERAKAVLKENNVRQVLCAFADVRGYLQMFSIPAREFVDGSAFENGIGFDGSSVRGFRTIEKSDMVWM
PDASSLKIIPWIDDPIQKSAIMFGNVYEAWGTEIADCDPRGYVAKRYEDMLKSEGMSAIFGPEIEFFLFEGVDFTRLSWD
MWVSPNGGAGDSWGPPRIMPISSELESGYMIRPKEGYFRPPPEDTTVEYRNELVYYLEQLGIDIEYHHHEVATAGQVELD
FKPKQLVDVGDAFYLYKFAAKNIAAMHGLYATFMPKPLYLDNASGMHTHQSLWKGEPFSGEAVFADPDDEYMLSQKARYY
IGGLLEHAKALTALCAPTVNSYKRLVPGFEAPIYICWSPRNRSALVRVPMYVKKPSAIRVEYRGVDPSCNPYLAITAQLA
AGLDGIKKKIDPGDPLLEDVYELTPAQKRELGVGELPTTLRDAIDHLASDELMQEVLGSHIFDAFMELKIDEWNQYCLYI
TPWEFMKYFDI

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family

Homologues:

Organism=Homo sapiens, GI221307485, Length=424, Percent_Identity=23.8207547169811, Blast_Score=105, Evalue=8e-23,
Organism=Escherichia coli, GI1790301, Length=487, Percent_Identity=40.6570841889117, Blast_Score=321, Evalue=8e-89,
Organism=Escherichia coli, GI87081870, Length=257, Percent_Identity=29.5719844357977, Blast_Score=98, Evalue=1e-21,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): GLNA_ARCFU (O29313)

Other databases:

- EMBL:   AE000782
- PIR:   E69368
- RefSeq:   NP_069782.1
- ProteinModelPortal:   O29313
- GeneID:   1484172
- GenomeReviews:   AE000782_GR
- KEGG:   afu:AF0949
- NMPDR:   fig|224325.1.peg.941
- TIGR:   AF_0949
- HOGENOM:   HBG553755
- OMA:   NIYLLEA
- ProtClustDB:   CLSK2746682
- BioCyc:   AFUL224325:AF_0949-MONOMER
- BRENDA:   6.3.1.2
- GO:   GO:0005737
- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- Gene3D:   G3DSA:3.30.590.10
- TIGRFAMs:   TIGR00653

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N; SSF54368 Gln_synt_beta

EC number: =6.3.1.2

Molecular weight: Translated: 55723; Mature: 55723

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00180 GLNA_1; PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRRLRGDCMEEVERAKAVLKENNVRQVLCAFADVRGYLQMFSIPAREFVDGSAFENGIG
CCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCC
FDGSSVRGFRTIEKSDMVWMPDASSLKIIPWIDDPIQKSAIMFGNVYEAWGTEIADCDPR
CCCCCCCCEEEECCCCEEECCCCCCEEEEECCCCHHHHHHHHCCCHHHHHCCCCCCCCCC
GYVAKRYEDMLKSEGMSAIFGPEIEFFLFEGVDFTRLSWDMWVSPNGGAGDSWGPPRIMP
CHHHHHHHHHHHHCCCCEEECCCEEEEEECCCCEEEEEEEEEECCCCCCCCCCCCCEEEE
ISSELESGYMIRPKEGYFRPPPEDTTVEYRNELVYYLEQLGIDIEYHHHEVATAGQVELD
ECHHHCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEECCCEECCCCEEEEC
FKPKQLVDVGDAFYLYKFAAKNIAAMHGLYATFMPKPLYLDNASGMHTHQSLWKGEPFSG
CCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHCCCCCCC
EAVFADPDDEYMLSQKARYYIGGLLEHAKALTALCAPTVNSYKRLVPGFEAPIYICWSPR
CEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCEEEEECCC
NRSALVRVPMYVKKPSAIRVEYRGVDPSCNPYLAITAQLAAGLDGIKKKIDPGDPLLEDV
CCCEEEEECEEEECCCEEEEEEECCCCCCCCEEEEHHHHHHCHHHHHHCCCCCCHHHHHH
YELTPAQKRELGVGELPTTLRDAIDHLASDELMQEVLGSHIFDAFMELKIDEWNQYCLYI
HHCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEEE
TPWEFMKYFDI
EHHHHHHHHCC
>Mature Secondary Structure
MVRRLRGDCMEEVERAKAVLKENNVRQVLCAFADVRGYLQMFSIPAREFVDGSAFENGIG
CCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCC
FDGSSVRGFRTIEKSDMVWMPDASSLKIIPWIDDPIQKSAIMFGNVYEAWGTEIADCDPR
CCCCCCCCEEEECCCCEEECCCCCCEEEEECCCCHHHHHHHHCCCHHHHHCCCCCCCCCC
GYVAKRYEDMLKSEGMSAIFGPEIEFFLFEGVDFTRLSWDMWVSPNGGAGDSWGPPRIMP
CHHHHHHHHHHHHCCCCEEECCCEEEEEECCCCEEEEEEEEEECCCCCCCCCCCCCEEEE
ISSELESGYMIRPKEGYFRPPPEDTTVEYRNELVYYLEQLGIDIEYHHHEVATAGQVELD
ECHHHCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEECCCEECCCCEEEEC
FKPKQLVDVGDAFYLYKFAAKNIAAMHGLYATFMPKPLYLDNASGMHTHQSLWKGEPFSG
CCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHCCCCCCC
EAVFADPDDEYMLSQKARYYIGGLLEHAKALTALCAPTVNSYKRLVPGFEAPIYICWSPR
CEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCEEEEECCC
NRSALVRVPMYVKKPSAIRVEYRGVDPSCNPYLAITAQLAAGLDGIKKKIDPGDPLLEDV
CCCEEEEECEEEECCCEEEEEEECCCCCCCCEEEEHHHHHHCHHHHHHCCCCCCHHHHHH
YELTPAQKRELGVGELPTTLRDAIDHLASDELMQEVLGSHIFDAFMELKIDEWNQYCLYI
HHCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEEE
TPWEFMKYFDI
EHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475