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The map label for this gene is purA

Identifier: 11498447

GI number: 11498447

Start: 748015

End: 749028

Strand: Reverse

Name: purA

Synonym: AF0841

Alternate gene names: 11498447

Gene position: 749028-748015 (Counterclockwise)

Preceding gene: 11498448

Following gene: 11498441

Centisome position: 34.38

GC content: 53.85

Gene sequence:

>1014_bases
ATGGGAGCTACGATAATTGTTGGCGGATTCTGGGGAGATGAGGGGAAGGGAAAGATAGTGGCTCACGTTGCACACAGCGA
TAAGCCTGTTATTATTGCCAGGGGAGGCGTAGGGCCAAATGCGGGCCACACAGTGGAGATTGACGGTCAGAAGTTTGGAG
TGAGGATGATTCCGTCGGGGTTCGTTTACAAGGACGCGAAACTGCTGATCGGGGCCGGAGTTCTCGTCAATCCCGAGGTT
TTTCTCAAAGAGGTTGAACTTCTGAAGGTTGGGGACAGGGCGAGGGTTGATTACAGGTGTGCGATAATTGAGCCGAAGCA
CATTGAGGCTGACAAGGGAAGCGAGCACCTGAGCAAGAAAATCGGCACGACCGGAACTGGCTGCGGGCCGGCGAATGTTG
ACAGGGTTAACAGAGTTGCAAAGCAGGCGAAGGACATTCCAGAACTCAAAGACTACCTTGCTGACGTCCCCCTTGAGGTG
AATCAAGCTATAGAGAACGGACAGTTTGTGCTGATAGAGGGGAGCCAGGGGTTCGGTCTCAGCCTGTACTACGGAACCTA
CCCGTATGTGACGTCTAAGGACACAACTGCCTCGGCCATAGCCTCGGACGTTGGGGTTGGGCCGACGAGGGTTGATGACG
TTATTGTCGTCTTCAAGTGCTTCCCGACGAGAGTTGGTGCTGGTCCGTTCCCCACGGAAATGCCTCAGGAGGAGGCCGAG
AAGCTCGGCATAGTTGAGTACGGCACCGTGACCGGAAGGAGGAGGAGAATAGGCTACTGGGACGGAGAGTTCGCTCGCTA
TTCCGCAATGGTTAACGGCGCAACGCAGGTTGCGATAACCGGAGTGGACAAGCTCGACAAGGAGTGCTACGGGGTTACGG
AGTGGGAGAAACTTACTCCCAAAGCGAAAAAGTTCATTGAGCAGGTTGAGGAGGACGTCAGGGTTCCGGTAACGCTCATC
TCCACCGGACCTGAGCTGAAGCAGATTATAGATTTAAGAAAAGAAAAGCTTTAA

Upstream 100 bases:

>100_bases
GCTTGCTGAGCTTCATTTGATCTATTAAACCTTAACTCAAACCTACCATTTTTCCAAAAATCGCAAACCAAATAAACCCC
TGCGAAGGACTGTCAGTGTT

Downstream 100 bases:

>100_bases
CTCTTTTTCTTTAATTTCTCTGCTTCCTCCTCCCTCAGAACCCTCCTCAGCACCTTCCCAACGAGGGACTTCGGAAGCTC
ATCTCTGAACTCGTACAGCC

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 337; Mature: 336

Protein sequence:

>337_residues
MGATIIVGGFWGDEGKGKIVAHVAHSDKPVIIARGGVGPNAGHTVEIDGQKFGVRMIPSGFVYKDAKLLIGAGVLVNPEV
FLKEVELLKVGDRARVDYRCAIIEPKHIEADKGSEHLSKKIGTTGTGCGPANVDRVNRVAKQAKDIPELKDYLADVPLEV
NQAIENGQFVLIEGSQGFGLSLYYGTYPYVTSKDTTASAIASDVGVGPTRVDDVIVVFKCFPTRVGAGPFPTEMPQEEAE
KLGIVEYGTVTGRRRRIGYWDGEFARYSAMVNGATQVAITGVDKLDKECYGVTEWEKLTPKAKKFIEQVEEDVRVPVTLI
STGPELKQIIDLRKEKL

Sequences:

>Translated_337_residues
MGATIIVGGFWGDEGKGKIVAHVAHSDKPVIIARGGVGPNAGHTVEIDGQKFGVRMIPSGFVYKDAKLLIGAGVLVNPEV
FLKEVELLKVGDRARVDYRCAIIEPKHIEADKGSEHLSKKIGTTGTGCGPANVDRVNRVAKQAKDIPELKDYLADVPLEV
NQAIENGQFVLIEGSQGFGLSLYYGTYPYVTSKDTTASAIASDVGVGPTRVDDVIVVFKCFPTRVGAGPFPTEMPQEEAE
KLGIVEYGTVTGRRRRIGYWDGEFARYSAMVNGATQVAITGVDKLDKECYGVTEWEKLTPKAKKFIEQVEEDVRVPVTLI
STGPELKQIIDLRKEKL
>Mature_336_residues
GATIIVGGFWGDEGKGKIVAHVAHSDKPVIIARGGVGPNAGHTVEIDGQKFGVRMIPSGFVYKDAKLLIGAGVLVNPEVF
LKEVELLKVGDRARVDYRCAIIEPKHIEADKGSEHLSKKIGTTGTGCGPANVDRVNRVAKQAKDIPELKDYLADVPLEVN
QAIENGQFVLIEGSQGFGLSLYYGTYPYVTSKDTTASAIASDVGVGPTRVDDVIVVFKCFPTRVGAGPFPTEMPQEEAEK
LGIVEYGTVTGRRRRIGYWDGEFARYSAMVNGATQVAITGVDKLDKECYGVTEWEKLTPKAKKFIEQVEEDVRVPVTLIS
TGPELKQIIDLRKEKL

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=432, Percent_Identity=28.0092592592593, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI34577063, Length=342, Percent_Identity=30.7017543859649, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI40316944, Length=398, Percent_Identity=26.6331658291457, Blast_Score=125, Evalue=8e-29,
Organism=Escherichia coli, GI1790620, Length=429, Percent_Identity=32.6340326340326, Blast_Score=192, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI25146553, Length=422, Percent_Identity=30.3317535545024, Blast_Score=172, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI25146555, Length=422, Percent_Identity=30.3317535545024, Blast_Score=171, Evalue=4e-43,
Organism=Saccharomyces cerevisiae, GI6324109, Length=428, Percent_Identity=29.6728971962617, Blast_Score=181, Evalue=1e-46,
Organism=Drosophila melanogaster, GI21356033, Length=336, Percent_Identity=31.25, Blast_Score=150, Evalue=1e-36,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_ARCFU (O29417)

Other databases:

- EMBL:   AE000782
- PIR:   A69355
- RefSeq:   NP_069675.1
- ProteinModelPortal:   O29417
- SMR:   O29417
- GeneID:   1484060
- GenomeReviews:   AE000782_GR
- KEGG:   afu:AF0841
- NMPDR:   fig|224325.1.peg.834
- TIGR:   AF_0841
- HOGENOM:   HBG658237
- OMA:   IDPHVGI
- ProtClustDB:   PRK04293
- BioCyc:   AFUL224325:AF_0841-MONOMER
- BRENDA:   6.3.4.4
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 36563; Mature: 36432

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 13-13 ACT_SITE 43-43 BINDING 124-124 BINDING 138-138 BINDING 176-176 BINDING 191-191 BINDING 253-253 BINDING 255-255

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGATIIVGGFWGDEGKGKIVAHVAHSDKPVIIARGGVGPNAGHTVEIDGQKFGVRMIPSG
CCCEEEEEEEECCCCCCEEEEEEECCCCCEEEEECCCCCCCCCEEEECCCEECEEECCCC
FVYKDAKLLIGAGVLVNPEVFLKEVELLKVGDRARVDYRCAIIEPKHIEADKGSEHLSKK
CEEECCEEEEECCEEECHHHHHHHHHHEECCCCCCEEEEEEEECCCCCCCCCCHHHHHHH
IGTTGTGCGPANVDRVNRVAKQAKDIPELKDYLADVPLEVNQAIENGQFVLIEGSQGFGL
CCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHCCCCEEEEECCCCCEE
SLYYGTYPYVTSKDTTASAIASDVGVGPTRVDDVIVVFKCFPTRVGAGPFPTEMPQEEAE
EEEECCCCEEECCCCHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHH
KLGIVEYGTVTGRRRRIGYWDGEFARYSAMVNGATQVAITGVDKLDKECYGVTEWEKLTP
HCCCEEECCCCCCCEECCCCCCCHHHHHHHHCCCEEEEEECHHHHHHHHCCCCHHHHCCH
KAKKFIEQVEEDVRVPVTLISTGPELKQIIDLRKEKL
HHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHCC
>Mature Secondary Structure 
GATIIVGGFWGDEGKGKIVAHVAHSDKPVIIARGGVGPNAGHTVEIDGQKFGVRMIPSG
CCEEEEEEEECCCCCCEEEEEEECCCCCEEEEECCCCCCCCCEEEECCCEECEEECCCC
FVYKDAKLLIGAGVLVNPEVFLKEVELLKVGDRARVDYRCAIIEPKHIEADKGSEHLSKK
CEEECCEEEEECCEEECHHHHHHHHHHEECCCCCCEEEEEEEECCCCCCCCCCHHHHHHH
IGTTGTGCGPANVDRVNRVAKQAKDIPELKDYLADVPLEVNQAIENGQFVLIEGSQGFGL
CCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHCCCCEEEEECCCCCEE
SLYYGTYPYVTSKDTTASAIASDVGVGPTRVDDVIVVFKCFPTRVGAGPFPTEMPQEEAE
EEEECCCCEEECCCCHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHH
KLGIVEYGTVTGRRRRIGYWDGEFARYSAMVNGATQVAITGVDKLDKECYGVTEWEKLTP
HCCCEEECCCCCCCEECCCCCCCHHHHHHHHCCCEEEEEECHHHHHHHHCCCCHHHHCCH
KAKKFIEQVEEDVRVPVTLISTGPELKQIIDLRKEKL
HHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475