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The map label for this gene is thi1

Identifier: 11498309

GI number: 11498309

Start: 633910

End: 634692

Strand: Reverse

Name: thi1

Synonym: AF0702

Alternate gene names: NA

Gene position: 634692-633910 (Counterclockwise)

Preceding gene: 11498310

Following gene: 11498308

Centisome position: 29.14

GC content: 52.75

Gene sequence:

>783_bases
ATGCCGCTAAAATTTACTGAGGTTATGGAAGCAGAAATCACAAAGGCAATAGTCGAAACCGCTTCGGAGGAATGGGTCGA
ATACGCGGAGTCCGACGTCATTGTTGTTGGTGCCGGGCCATCGGGGCTAACCGCTGCGAGATACCTTGCGGAAAAGGGCC
TTAAGACGCTCGTTCTTGAGCGCAGGCTGAGTTTTGGCGGTGGGATAGGCGGAGGAGGAATGCTCTTTCATAAAGTCGTC
GTGGAGAGGGAGGCGAAGGACATCCTCGACGACTTTGGTATCAGGTACACCGAGCACAGAAACTTTCTCGTTGCGGATTC
TGCCGAGTTCATGGCAAAGCTCGCCGCAAAGGCCATTGATGCCGGAGCAAAGATAATTCACGGAGTTTCGGTAGAGGACG
TTATATTCAGGGACGACCCGCTTGGGGTTAGGGGTGTTTGCATTCAGTGGAGCGCGGTTGAGATTTCTGGACTGCACGTT
GACCCGCTTTTCCTGAGAAGCAGGGCTGTTGTGGATGCAACCGGGCATGATGCGGAGGTTATATCCGTTGCTGCAAGAAA
GATACCGCTGGAAGTCTCCGTTGTCGGCGAAAGGTCCGCTTACTCCGAAGTTGCGGAGAGGGAAATCGTGGAAAAGACTG
GAAAAATCGTTAAAGGGCTTTACGCTGCAGGAATGGCCGTTGCAGCAGTCCATAACCTGCCGAGAATGGGGCCGATTTTT
GGCGGAATGCTGCTGAGCGGAAAGAAGGTTGCGGAAATAGTTGCTGAAGACCTGAAGTTATGA

Upstream 100 bases:

>100_bases
TTTCGCAAAGTTCTACGGAATTGGCCGTGAGCCTCATAGGATTCGTGGGGCTGATGTACTACTTTTTGAGGGGCTAAAGC
GGGTACGATGAAAGTTATAA

Downstream 100 bases:

>100_bases
GGGTGGTTTACACATCCCCGAGGATTGAGAATCTCCAATTTTTAAAATCGGAGTTCTCGAATTACAGCGTGATAGTTCTC
GAAGAGCCGAAAAACGACCT

Product: ribulose-1,5-biphosphate synthetase

Products: NA

Alternate protein names: Ribulose 1,5-bisphosphate synthase; RuBP synthase

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MPLKFTEVMEAEITKAIVETASEEWVEYAESDVIVVGAGPSGLTAARYLAEKGLKTLVLERRLSFGGGIGGGGMLFHKVV
VEREAKDILDDFGIRYTEHRNFLVADSAEFMAKLAAKAIDAGAKIIHGVSVEDVIFRDDPLGVRGVCIQWSAVEISGLHV
DPLFLRSRAVVDATGHDAEVISVAARKIPLEVSVVGERSAYSEVAEREIVEKTGKIVKGLYAAGMAVAAVHNLPRMGPIF
GGMLLSGKKVAEIVAEDLKL

Sequences:

>Translated_260_residues
MPLKFTEVMEAEITKAIVETASEEWVEYAESDVIVVGAGPSGLTAARYLAEKGLKTLVLERRLSFGGGIGGGGMLFHKVV
VEREAKDILDDFGIRYTEHRNFLVADSAEFMAKLAAKAIDAGAKIIHGVSVEDVIFRDDPLGVRGVCIQWSAVEISGLHV
DPLFLRSRAVVDATGHDAEVISVAARKIPLEVSVVGERSAYSEVAEREIVEKTGKIVKGLYAAGMAVAAVHNLPRMGPIF
GGMLLSGKKVAEIVAEDLKL
>Mature_259_residues
PLKFTEVMEAEITKAIVETASEEWVEYAESDVIVVGAGPSGLTAARYLAEKGLKTLVLERRLSFGGGIGGGGMLFHKVVV
EREAKDILDDFGIRYTEHRNFLVADSAEFMAKLAAKAIDAGAKIIHGVSVEDVIFRDDPLGVRGVCIQWSAVEISGLHVD
PLFLRSRAVVDATGHDAEVISVAARKIPLEVSVVGERSAYSEVAEREIVEKTGKIVKGLYAAGMAVAAVHNLPRMGPIFG
GMLLSGKKVAEIVAEDLKL

Specific function: Catalyzes the conversion of ribose 1,5-bisphosphate to ribulose 1,5-bisphosphate (RuBP), the CO(2) acceptor and substrate for RubisCO

COG id: COG1635

COG function: function code H; Flavoprotein involved in thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the THI4 family

Homologues:

Organism=Saccharomyces cerevisiae, GI6321583, Length=283, Percent_Identity=29.3286219081272, Blast_Score=102, Evalue=9e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUBPS_ARCFU (O29556)

Other databases:

- EMBL:   AE000782
- PIR:   F69337
- RefSeq:   NP_069536.1
- ProteinModelPortal:   O29556
- SMR:   O29556
- GeneID:   1483920
- GenomeReviews:   AE000782_GR
- KEGG:   afu:AF0702
- NMPDR:   fig|224325.1.peg.695
- TIGR:   AF_0702
- HOGENOM:   HBG325902
- OMA:   GLHVDPL
- ProtClustDB:   PRK04176
- BioCyc:   AFUL224325:AF_0702-MONOMER
- HAMAP:   MF_00304
- InterPro:   IPR013027
- InterPro:   IPR002922
- InterPro:   IPR022828
- PRINTS:   PR00368
- TIGRFAMs:   TIGR00292

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 27858; Mature: 27726

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLKFTEVMEAEITKAIVETASEEWVEYAESDVIVVGAGPSGLTAARYLAEKGLKTLVLE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHH
RRLSFGGGIGGGGMLFHKVVVEREAKDILDDFGIRYTEHRNFLVADSAEFMAKLAAKAID
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEECCCCEEEECCHHHHHHHHHHHHH
AGAKIIHGVSVEDVIFRDDPLGVRGVCIQWSAVEISGLHVDPLFLRSRAVVDATGHDAEV
CCHHHHCCCCHHHEEECCCCCCCCEEEEEEEEEEEECCEECHHHHHCCEEEECCCCCHHH
ISVAARKIPLEVSVVGERSAYSEVAEREIVEKTGKIVKGLYAAGMAVAAVHNLPRMGPIF
HHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
GGMLLSGKKVAEIVAEDLKL
HHHHHCCHHHHHHHHHHHCC
>Mature Secondary Structure 
PLKFTEVMEAEITKAIVETASEEWVEYAESDVIVVGAGPSGLTAARYLAEKGLKTLVLE
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHH
RRLSFGGGIGGGGMLFHKVVVEREAKDILDDFGIRYTEHRNFLVADSAEFMAKLAAKAID
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEECCCCEEEECCHHHHHHHHHHHHH
AGAKIIHGVSVEDVIFRDDPLGVRGVCIQWSAVEISGLHVDPLFLRSRAVVDATGHDAEV
CCHHHHCCCCHHHEEECCCCCCCCEEEEEEEEEEEECCEECHHHHHCCEEEECCCCCHHH
ISVAARKIPLEVSVVGERSAYSEVAEREIVEKTGKIVKGLYAAGMAVAAVHNLPRMGPIF
HHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
GGMLLSGKKVAEIVAEDLKL
HHHHHCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475