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The map label for this gene is gspE-2

Identifier: 11498267

GI number: 11498267

Start: 593473

End: 595008

Strand: Reverse

Name: gspE-2

Synonym: AF0659

Alternate gene names: 11498267

Gene position: 595008-593473 (Counterclockwise)

Preceding gene: 11498268

Following gene: 11498266

Centisome position: 27.31

GC content: 48.7

Gene sequence:

>1536_bases
ATGGCAAAAAACCACTACGATATACTTAGAAGGCACATCAGGAGTGAGGACTTGCTTGAGACACCAGAGTTCGGTAGCGG
TAGCAGGATCGTGGAAGAGTACTGGATACAGGAACCATTCACAAAGGCCATAATTGTTGAAAACGAGGATGAGTTCAGAA
ACGTCTATTACGCTCTCGAACCGACTGTAAGCTCCGAGGAGGCGGAAGTAATCTCCGCCCTTTACGATGACCTGAAGAAA
ATTTTGGTTCTGCAGGACGTCTCTGTTGATCTGGAAGAGAGAGCAGAAGTGCTTGTCAGAGCGATTGAAAAGCTCTCCAA
GGAGTACGCTGTGAGTTTTACTGACAACTTCTACTCGAGAATGCTTTACTACCTTTTCCGCGACTTTTTCGGTTACGGTT
TGATAGACCCCTTGATGGAGGACACCAATGTTGAGGATATCTCGTGTGATGGCTACAACATTCCAATTTTCATATACCAC
CAGAAGTACGGAAATGTGGAGACGAACATCGTTCTTGATCAGGAAAAGCTTGACAGGATGGTTCTGAGGCTGACGCAGAG
AAGCGGGAAGCACATTTCAATAGCAAATCCGATTGTTGATGCCACGCTTCCCGACGGGAGCAGGTTGCAGGCGACCTTTG
GCACCGAAGTAACGCCTCGGGGTTCAAGTTTTACGATAAGGAAGTTCACGATTGAACCGCTCACACCCATAGATTTGATA
GAAAAGGGAACAGTTCCTTCAGGAGTTCTCGCCTACCTCTGGCTGGCGATAGAGCACAAGTTCTCCGCAATCGTTGTGGG
AGAGACTGCGAGCGGAAAGACGACCACGCTAAACGCCATTATGATGTTCATTCCCCCGGATGCAAAGGTCGTCTCCATCG
AGGATACGAGAGAGATAAAGCTCTACCACGAAAACTGGATCGCAGAGGTTACGAGGACGGGAATGGGTGAGGGAGAAATA
GACATGTACGACCTTCTGAGGGCTGCGCTAAGGCAGAGACCCGACTACATCATTGTGGGTGAGGTTAGAGGAAGGGAGGC
ACAAACGCTCTTCCAGGCTATGTCTACCGGCCACGCGAGCTACTCCACACTCCATGCCGGAGATATCAACCAGATGGTTT
ACAGACTCGAATCTGAGCCGCTTAAGGTGCCGAGAAGCATGCTTCAGTTCCTCGATATCGCTCTCGTGCAGACGATGTGG
GTGAGGGGAAACACAAGGCTAAGGAGGACGAAGGAGGTTAACGAAATCCTCGGCATAGACCCTGTTGACAAAAACCTTCT
GGTGAACCAGTTTGTTAAGTGGGACCCTAAGGAGGATAAGCACATTGAGGTTAGCATGCCTAAGAAGCTTGAAAAGATGG
CCGATTTCTTAGGTGTCAGCGTTCAGGAGGTTTACGATGAAATGCTGAGCAGAAAGAGGTACCTTGAACTCATGCTGAAA
AGGGGAATCAGAAACTACAAAGAGGTTACGAGATATATCCACGCCTACTATCGCAATCCTGAGCTTGCTATGACGAAAAT
GGAGGAAGGCCTATGA

Upstream 100 bases:

>100_bases
AGAGAAGTACGTAAGGGGCGACCCCAGCCCCCGCTGGGACCTGCCTGACTTGTAAATTTGCAAGAAAAGCATATATTTTG
GGAAATATAACATTTAGTCA

Downstream 100 bases:

>100_bases
GCCTCTACACTCCATTGGCGCTGAGGAGATTTTACAGGAAAAGATACATCCGAAGACCGGGAAGCTACTCGGAGCTGTCT
TCAGCGATAGAAAGCGCGAG

Product: type II secretion system protein (gspE-2)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 511; Mature: 510

Protein sequence:

>511_residues
MAKNHYDILRRHIRSEDLLETPEFGSGSRIVEEYWIQEPFTKAIIVENEDEFRNVYYALEPTVSSEEAEVISALYDDLKK
ILVLQDVSVDLEERAEVLVRAIEKLSKEYAVSFTDNFYSRMLYYLFRDFFGYGLIDPLMEDTNVEDISCDGYNIPIFIYH
QKYGNVETNIVLDQEKLDRMVLRLTQRSGKHISIANPIVDATLPDGSRLQATFGTEVTPRGSSFTIRKFTIEPLTPIDLI
EKGTVPSGVLAYLWLAIEHKFSAIVVGETASGKTTTLNAIMMFIPPDAKVVSIEDTREIKLYHENWIAEVTRTGMGEGEI
DMYDLLRAALRQRPDYIIVGEVRGREAQTLFQAMSTGHASYSTLHAGDINQMVYRLESEPLKVPRSMLQFLDIALVQTMW
VRGNTRLRRTKEVNEILGIDPVDKNLLVNQFVKWDPKEDKHIEVSMPKKLEKMADFLGVSVQEVYDEMLSRKRYLELMLK
RGIRNYKEVTRYIHAYYRNPELAMTKMEEGL

Sequences:

>Translated_511_residues
MAKNHYDILRRHIRSEDLLETPEFGSGSRIVEEYWIQEPFTKAIIVENEDEFRNVYYALEPTVSSEEAEVISALYDDLKK
ILVLQDVSVDLEERAEVLVRAIEKLSKEYAVSFTDNFYSRMLYYLFRDFFGYGLIDPLMEDTNVEDISCDGYNIPIFIYH
QKYGNVETNIVLDQEKLDRMVLRLTQRSGKHISIANPIVDATLPDGSRLQATFGTEVTPRGSSFTIRKFTIEPLTPIDLI
EKGTVPSGVLAYLWLAIEHKFSAIVVGETASGKTTTLNAIMMFIPPDAKVVSIEDTREIKLYHENWIAEVTRTGMGEGEI
DMYDLLRAALRQRPDYIIVGEVRGREAQTLFQAMSTGHASYSTLHAGDINQMVYRLESEPLKVPRSMLQFLDIALVQTMW
VRGNTRLRRTKEVNEILGIDPVDKNLLVNQFVKWDPKEDKHIEVSMPKKLEKMADFLGVSVQEVYDEMLSRKRYLELMLK
RGIRNYKEVTRYIHAYYRNPELAMTKMEEGL
>Mature_510_residues
AKNHYDILRRHIRSEDLLETPEFGSGSRIVEEYWIQEPFTKAIIVENEDEFRNVYYALEPTVSSEEAEVISALYDDLKKI
LVLQDVSVDLEERAEVLVRAIEKLSKEYAVSFTDNFYSRMLYYLFRDFFGYGLIDPLMEDTNVEDISCDGYNIPIFIYHQ
KYGNVETNIVLDQEKLDRMVLRLTQRSGKHISIANPIVDATLPDGSRLQATFGTEVTPRGSSFTIRKFTIEPLTPIDLIE
KGTVPSGVLAYLWLAIEHKFSAIVVGETASGKTTTLNAIMMFIPPDAKVVSIEDTREIKLYHENWIAEVTRTGMGEGEID
MYDLLRAALRQRPDYIIVGEVRGREAQTLFQAMSTGHASYSTLHAGDINQMVYRLESEPLKVPRSMLQFLDIALVQTMWV
RGNTRLRRTKEVNEILGIDPVDKNLLVNQFVKWDPKEDKHIEVSMPKKLEKMADFLGVSVQEVYDEMLSRKRYLELMLKR
GIRNYKEVTRYIHAYYRNPELAMTKMEEGL

Specific function: Unknown

COG id: COG0630

COG function: function code NU; Type IV secretory pathway, VirB11 components, and related ATPases involved in archaeal flagella biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1786296, Length=170, Percent_Identity=31.1764705882353, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1789723, Length=121, Percent_Identity=38.0165289256198, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 58980; Mature: 58849

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKNHYDILRRHIRSEDLLETPEFGSGSRIVEEYWIQEPFTKAIIVENEDEFRNVYYALE
CCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCCEEEEEECCHHHCEEEEEEC
PTVSSEEAEVISALYDDLKKILVLQDVSVDLEERAEVLVRAIEKLSKEYAVSFTDNFYSR
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHEEEHHHHHHHH
MLYYLFRDFFGYGLIDPLMEDTNVEDISCDGYNIPIFIYHQKYGNVETNIVLDQEKLDRM
HHHHHHHHHHCCCHHHHHHCCCCCCEEECCCCCCCEEEEEECCCCCEEEEEECHHHHHHH
VLRLTQRSGKHISIANPIVDATLPDGSRLQATFGTEVTPRGSSFTIRKFTIEPLTPIDLI
HHHHHHCCCCEEEECCCEEECCCCCCCEEEEECCCEECCCCCCEEEEEEEECCCCHHHHH
EKGTVPSGVLAYLWLAIEHKFSAIVVGETASGKTTTLNAIMMFIPPDAKVVSIEDTREIK
HCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEEEEEECCCCEEEEECCCCEEE
LYHENWIAEVTRTGMGEGEIDMYDLLRAALRQRPDYIIVGEVRGREAQTLFQAMSTGHAS
EEHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCCC
YSTLHAGDINQMVYRLESEPLKVPRSMLQFLDIALVQTMWVRGNTRLRRTKEVNEILGID
CCEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCC
PVDKNLLVNQFVKWDPKEDKHIEVSMPKKLEKMADFLGVSVQEVYDEMLSRKRYLELMLK
CCCHHHHHHHHHCCCCCCCCEEEEECHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
RGIRNYKEVTRYIHAYYRNPELAMTKMEEGL
HHHHHHHHHHHHHHHHHCCCCHHHHHHHCCC
>Mature Secondary Structure 
AKNHYDILRRHIRSEDLLETPEFGSGSRIVEEYWIQEPFTKAIIVENEDEFRNVYYALE
CCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCCEEEEEECCHHHCEEEEEEC
PTVSSEEAEVISALYDDLKKILVLQDVSVDLEERAEVLVRAIEKLSKEYAVSFTDNFYSR
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHEEEHHHHHHHH
MLYYLFRDFFGYGLIDPLMEDTNVEDISCDGYNIPIFIYHQKYGNVETNIVLDQEKLDRM
HHHHHHHHHHCCCHHHHHHCCCCCCEEECCCCCCCEEEEEECCCCCEEEEEECHHHHHHH
VLRLTQRSGKHISIANPIVDATLPDGSRLQATFGTEVTPRGSSFTIRKFTIEPLTPIDLI
HHHHHHCCCCEEEECCCEEECCCCCCCEEEEECCCEECCCCCCEEEEEEEECCCCHHHHH
EKGTVPSGVLAYLWLAIEHKFSAIVVGETASGKTTTLNAIMMFIPPDAKVVSIEDTREIK
HCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEEEEEECCCCEEEEECCCCEEE
LYHENWIAEVTRTGMGEGEIDMYDLLRAALRQRPDYIIVGEVRGREAQTLFQAMSTGHAS
EEHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCCC
YSTLHAGDINQMVYRLESEPLKVPRSMLQFLDIALVQTMWVRGNTRLRRTKEVNEILGID
CCEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCC
PVDKNLLVNQFVKWDPKEDKHIEVSMPKKLEKMADFLGVSVQEVYDEMLSRKRYLELMLK
CCCHHHHHHHHHCCCCCCCCEEEEECHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
RGIRNYKEVTRYIHAYYRNPELAMTKMEEGL
HHHHHHHHHHHHHHHHHCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]