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The map label for this gene is nrtB-2

Identifier: 11498247

GI number: 11498247

Start: 576332

End: 577078

Strand: Reverse

Name: nrtB-2

Synonym: AF0639

Alternate gene names: 11498247

Gene position: 577078-576332 (Counterclockwise)

Preceding gene: 11498248

Following gene: 11498246

Centisome position: 26.49

GC content: 51.0

Gene sequence:

>747_bases
ATGAGGCTGAAGGGAGCGTTTGTACTCATTTTTTCCTGGTATCTGATTTCCGCCCTAACCGACCTCCCCCACCCCCACGA
GGTTGCGGAGGTTTTTTTCACGCTGCTTGTTAACCCAGAGCCGGTTCTTGGCAAGACCCTCGTTCAGCACGCTGTTGCGA
GTCTTTTCAGAGTTCTTGCGGCCTCTTTTCTTGCCTTCTCTATTGCAGTACCTCTCGGAATAATTTCGGGGTGGAACAGA
ACGCTTATGGAGTTTATTGCACCCGCAGTTGAGGCTTTGCGCCCCATACCCCCCTTGGCTTGGATTCCGCTCGCCTACAT
AATCTTTGCTCCACTGCCCAATACCGTTGAAGTTTCCCAGATTTTCATAGTCTTTGTTGGAGCTTTCTTCCCCTGCTTCA
TCTCCGTTTTTGACTACGCAAGAAATGTCCCGGAGGAGCTTTTGGAGATGGCCGAGGTCTTCAAGGCTCCTGAAAGGCTA
ATAATCACGGACATAGTCGTACCACACTCCCTGCAGGGGATTATGAGCGGTGTCAGAATTGGTCTCGGTGTGGGATGGAT
GAGCATCATTGCAGCAGAGATGATTGCTACCTCCGGAGAGGGCTTGGGTTACTTCATTATGGTAATGTACAATGTTGGCG
GGAGGACTGCTGAAATTGTTGCGGGCATGGCGATGATTGGAATTATTGGGTACATGATGAACTGGCTGCTTCTGAGGGCT
GAGAGGTGGGTGATGCCGTGGCGCTAA

Upstream 100 bases:

>100_bases
AAGAGCTTGCAGGGAAGAAGGTTGCCATTCCGGGCTTTGGAACAGTTCAGGACTTTCTGCTCAGAATGGCAGCTGAAAAG
GCGGGGCTGGAGATAACGAC

Downstream 100 bases:

>100_bases
TTGTAGAGGGGCTGAAGAAAAGCTACGGAAATCTGAGGGTTCTTGACGGGGTGACTTTCAGCGTTGAGAAGGGGGAGTTT
GTCTGCCTTATTGGGGAGAG

Product: nitrate ABC transporter, permease protein (nrtB-2)

Products: taurine [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MRLKGAFVLIFSWYLISALTDLPHPHEVAEVFFTLLVNPEPVLGKTLVQHAVASLFRVLAASFLAFSIAVPLGIISGWNR
TLMEFIAPAVEALRPIPPLAWIPLAYIIFAPLPNTVEVSQIFIVFVGAFFPCFISVFDYARNVPEELLEMAEVFKAPERL
IITDIVVPHSLQGIMSGVRIGLGVGWMSIIAAEMIATSGEGLGYFIMVMYNVGGRTAEIVAGMAMIGIIGYMMNWLLLRA
ERWVMPWR

Sequences:

>Translated_248_residues
MRLKGAFVLIFSWYLISALTDLPHPHEVAEVFFTLLVNPEPVLGKTLVQHAVASLFRVLAASFLAFSIAVPLGIISGWNR
TLMEFIAPAVEALRPIPPLAWIPLAYIIFAPLPNTVEVSQIFIVFVGAFFPCFISVFDYARNVPEELLEMAEVFKAPERL
IITDIVVPHSLQGIMSGVRIGLGVGWMSIIAAEMIATSGEGLGYFIMVMYNVGGRTAEIVAGMAMIGIIGYMMNWLLLRA
ERWVMPWR
>Mature_248_residues
MRLKGAFVLIFSWYLISALTDLPHPHEVAEVFFTLLVNPEPVLGKTLVQHAVASLFRVLAASFLAFSIAVPLGIISGWNR
TLMEFIAPAVEALRPIPPLAWIPLAYIIFAPLPNTVEVSQIFIVFVGAFFPCFISVFDYARNVPEELLEMAEVFKAPERL
IITDIVVPHSLQGIMSGVRIGLGVGWMSIIAAEMIATSGEGLGYFIMVMYNVGGRTAEIVAGMAMIGIIGYMMNWLLLRA
ERWVMPWR

Specific function: Probably part of a binding-protein-dependent transport system. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0600

COG function: function code P; ABC-type nitrate/sulfonate/bicarbonate transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786564, Length=204, Percent_Identity=33.3333333333333, Blast_Score=110, Evalue=9e-26,
Organism=Escherichia coli, GI87081802, Length=186, Percent_Identity=33.3333333333333, Blast_Score=77, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 27469; Mature: 27469

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHCCCCC
>Mature Secondary Structure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HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: taurine [Periplasm]; ATP; H2O [C]

Specific reaction: taurine [Periplasm] + ATP + H2O = taurine [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]