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Identifier: 11498052

GI number: 11498052

Start: 396387

End: 396734

Strand: Reverse

Name: Not Available

Synonym: AF0440

Alternate gene names: 11498052

Gene position: 396734-396387 (Counterclockwise)

Preceding gene: 11498053

Following gene: 11498051

Centisome position: 18.21

GC content: 47.7

Gene sequence:

>348_bases
ATGGAAGAGGAGATGATAATTGGCATATCCACATCCTTCAGCGCAGCACACAGCATCCCGGGCCACAAAAAGTGTGGAAA
AGTTCACGGCCACAACTTCAAGGTTGAAGTGGAGATATCGGGCAAAGTAAAGGAGAACGGGATGGTCATGGACTTCTTCG
ACCTAAAGAGAATTGTCAACGAGGTGGTGGCTAAGTTCGACCACACACTGCTCAACGAGCAGATCGAAATTCCAACCTCG
GAGAACATCTGCCTGAGAATATTCAGCGAACTTGCGGAAAAGGGACTTAACGTTCGCAGAGTGAGAGTTGCTGAAAATGA
AGACAAATGGGCGGAAATTCGAAGGTGA

Upstream 100 bases:

>100_bases
GCAGCAGGGTGGAAAAAATTCTTAAGCTGCAGAAGCGTATGATTGACTTCGCCGACACGAGGGTGATTCCGCAGGTTCAC
AAGTATCTGGGGGTGAGATG

Downstream 100 bases:

>100_bases
GATACTTACACTTTAATTGCAATTAAAATTGCCATTTAATGGCAAACATAAAATTTTTATATTTTTAGTTTACTTTCATA
CTATGCGAAAAGGCCAGCTT

Product: hypothetical protein

Products: 6-pyruvoyl-5,6,7,8-tetrahydropterin; triphosphate

Alternate protein names: CPH4 synthase; Archaeosine biosynthesis protein queD

Number of amino acids: Translated: 115; Mature: 115

Protein sequence:

>115_residues
MEEEMIIGISTSFSAAHSIPGHKKCGKVHGHNFKVEVEISGKVKENGMVMDFFDLKRIVNEVVAKFDHTLLNEQIEIPTS
ENICLRIFSELAEKGLNVRRVRVAENEDKWAEIRR

Sequences:

>Translated_115_residues
MEEEMIIGISTSFSAAHSIPGHKKCGKVHGHNFKVEVEISGKVKENGMVMDFFDLKRIVNEVVAKFDHTLLNEQIEIPTS
ENICLRIFSELAEKGLNVRRVRVAENEDKWAEIRR
>Mature_115_residues
MEEEMIIGISTSFSAAHSIPGHKKCGKVHGHNFKVEVEISGKVKENGMVMDFFDLKRIVNEVVAKFDHTLLNEQIEIPTS
ENICLRIFSELAEKGLNVRRVRVAENEDKWAEIRR

Specific function: Catalyzes the conversion of 7,8-dihydroneopterin triphosphate (H2NTP) to 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) and acetaldehyde

COG id: COG0720

COG function: function code H; 6-pyruvoyl-tetrahydropterin synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTPS family. QueD subfamily

Homologues:

Organism=Escherichia coli, GI1789124, Length=87, Percent_Identity=39.0804597701149, Blast_Score=61, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUED_ARCFU (O29809)

Other databases:

- EMBL:   AE000782
- PIR:   H69304
- RefSeq:   NP_069276.1
- ProteinModelPortal:   O29809
- SMR:   O29809
- GeneID:   1483656
- GenomeReviews:   AE000782_GR
- KEGG:   afu:AF0440
- NMPDR:   fig|224325.1.peg.435
- TIGR:   AF_0440
- HOGENOM:   HBG727155
- OMA:   IDSAHYL
- ProtClustDB:   CLSK2746434
- BioCyc:   AFUL224325:AF_0440-MONOMER
- BRENDA:   4.2.3.12
- InterPro:   IPR007115
- PANTHER:   PTHR12589

Pfam domain/function: PF01242 PTPS

EC number: 4.2.3.12

Molecular weight: Translated: 13150; Mature: 13150

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: ACT_SITE 25-25 ACT_SITE 68-68 ACT_SITE 105-105

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEEMIIGISTSFSAAHSIPGHKKCGKVHGHNFKVEVEISGKVKENGMVMDFFDLKRIVN
CCCCEEEEEECCCCHHHCCCCCCCCCCCCCCCEEEEEEECCEECCCCEEEEHHHHHHHHH
EVVAKFDHTLLNEQIEIPTSENICLRIFSELAEKGLNVRRVRVAENEDKWAEIRR
HHHHHHHHHHHCCEECCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCHHHHCCC
>Mature Secondary Structure
MEEEMIIGISTSFSAAHSIPGHKKCGKVHGHNFKVEVEISGKVKENGMVMDFFDLKRIVN
CCCCEEEEEECCCCHHHCCCCCCCCCCCCCCCEEEEEEECCEECCCCEEEEHHHHHHHHH
EVVAKFDHTLLNEQIEIPTSENICLRIFSELAEKGLNVRRVRVAENEDKWAEIRR
HHHHHHHHHHHCCEECCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Mg2+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 17.7 {6-(L-erythro-1,2-dihydroxypropyl} 5 {6-(L-erythro-1,2-dihydroxypropyl} 0.0091 {6-(L-erythro-1,2-dihydroxypropyl} 0.008 {6-(L-erythro-1,2-dihydroxypropyl} [C]

Substrates: 6-[(1S,2R)-1,2-dihydroxy-3-triphosphooxypropyl]-7,8-dihydropterin

Specific reaction: 6-[(1S,2R)-1,2-dihydroxy-3-triphosphooxypropyl]-7,8-dihydropterin = 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate

General reaction: Intramolecular redox reaction; P-O bond cleavage; Triphosphate elimination [C]

Inhibitor: (NH4)2SO4 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475