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The map label for this gene is leuA-2

Identifier: 11497835

GI number: 11497835

Start: 195581

End: 197092

Strand: Reverse

Name: leuA-2

Synonym: AF0219

Alternate gene names: 11497835

Gene position: 197092-195581 (Counterclockwise)

Preceding gene: 11497837

Following gene: 11497834

Centisome position: 9.05

GC content: 50.99

Gene sequence:

>1512_bases
ATGTCAGCAGTGATGACGACAATCAGAGTTTTCGACACCACCCTGAGAGATGGAGAGCAGATGCCCGGAGTTTCGCTCCC
CCTTGAATACAAAATCAGAATAGCCAAGCAACTCGACAAGCTCGGCGTGGATGTTATCGAGGCCGGATTCCCTTCAGCAA
CGAAGGGGGAGTTCGAGTCGGTTAAGGAGATAAGCTCGCTCAGCCTTAATGCTGAAATTTGCGGTCTTGCGAGAATCGTT
AAGGAGGATATTGATGCTGCCATTAACGCTAACGTTGATATGGTTCACATTTTCGTCCCCACCTCCAGAATACAGATAGA
GCACACCGTAAAGATGTCGAAAGAAGAGATTATTGAAAAATCGGTCGAATGCGTTGAGTACATCAAATCTCACGGCGTAA
AATGCATGTTCTCCGCGATGGATGCGACAAGGACGGAGGTTGAATACCTCAAGCAGATTTTCAAGGCCGTTGAAAATGCA
AAAGTTGACATCGTGAATGTTCCAGACACCGTCGGTGTAGCGACGCCCTTCAAGTTTTACGAGCTCATCAAGCAGCTGAG
GGAGCACCTCAAGGTTCCGATAGACGTCCACTGCCACAACGATTTCGGGCTGGCGGTTGCGAACACCTACGCGGCAGTGA
TGGCCGGAGCCAATGAGGTGCAGGTGACAGTGAACGGAATTGGGGAGAGGGCCGGTAACGCTGACCTCGCGCAGGTTGTG
ATGATTCTCCACGCAATCGAGGGGATAAAGACGAACATCAAAACGGAGCACCTCTTCGAGACCTCAAAGCTCGTTGAGAG
GCTCACCGGAGTTAAGATGCCACCAAACCACCCGATTGTGGGGGAGAACGCATTCAGCCACGAGAGCGGGATACACGCCC
ACGGAGTGCTGAAAGAGTACTCAACCTTCGAGCCGGGTGTAGTTACGCCGGAGATGGTCGGACATAAAAGGAGAATCGTC
ATAGGAAAGCATGCGGGGAGGTATCAAATAAAGAAGATTCTTGAGGAGGCCGGCTACCATCTTGACGACGAGAAGCTGAA
CCAGATATTCGAGAAGGTAAAGGAGATGGGGGACAAGGGGAAGAGAGTTACAGACCTGGATTTATTTGCCATAGCTGAAG
TGGTCATAGGCGAACTGAAGAGGGAAGAGAAGGCCATTCTCGTTGACGAGGTTACAGTTTTGACCGGCAACAAAATCACG
CCAACCGCAGTTCTGAACGCCGAGGTTTTCGGTCACAGAAAGGTCACCTCAGCAATCGGTGTGGGGCCTGTTGACGCCTC
CCTCAAGGCTGTTACATCTCTGGTTGGGGAGAGCATAAGAATCACGGAATTCAGAATGGACGCCATAACTGGAGGCAGCG
ATGCTCTTGCTGAGGTTTACGTAACGGTTGAAGACGATGAAGGGCATTCGTTCACATCCAGAGGAGCGGCACAGGACATA
GTGATGGCCTCAATCGATGCGGTCATCAATGCAGTTAACTACCTCCTGAGGATGAAGAGGAGAAAGAGCTGA

Upstream 100 bases:

>100_bases
CAACAAGAATACGTATTTGATTTGTAGCAGTTGCCGAGAATTTTATTTATTTCGATAAATTGTTATCTAAGGCACCGAAA
ACGATTTACAGCTTAGCCTA

Downstream 100 bases:

>100_bases
TGTACATTGTCGTATCAACCGGAGACAGAGCAGCAAGGATAGCCTCAAAGCTACCAGACTGCATAATCATAGACAGAGAC
CCTGACTCAGCCGAGAGGCT

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase 2; Alpha-isopropylmalate synthase 2

Number of amino acids: Translated: 503; Mature: 502

Protein sequence:

>503_residues
MSAVMTTIRVFDTTLRDGEQMPGVSLPLEYKIRIAKQLDKLGVDVIEAGFPSATKGEFESVKEISSLSLNAEICGLARIV
KEDIDAAINANVDMVHIFVPTSRIQIEHTVKMSKEEIIEKSVECVEYIKSHGVKCMFSAMDATRTEVEYLKQIFKAVENA
KVDIVNVPDTVGVATPFKFYELIKQLREHLKVPIDVHCHNDFGLAVANTYAAVMAGANEVQVTVNGIGERAGNADLAQVV
MILHAIEGIKTNIKTEHLFETSKLVERLTGVKMPPNHPIVGENAFSHESGIHAHGVLKEYSTFEPGVVTPEMVGHKRRIV
IGKHAGRYQIKKILEEAGYHLDDEKLNQIFEKVKEMGDKGKRVTDLDLFAIAEVVIGELKREEKAILVDEVTVLTGNKIT
PTAVLNAEVFGHRKVTSAIGVGPVDASLKAVTSLVGESIRITEFRMDAITGGSDALAEVYVTVEDDEGHSFTSRGAAQDI
VMASIDAVINAVNYLLRMKRRKS

Sequences:

>Translated_503_residues
MSAVMTTIRVFDTTLRDGEQMPGVSLPLEYKIRIAKQLDKLGVDVIEAGFPSATKGEFESVKEISSLSLNAEICGLARIV
KEDIDAAINANVDMVHIFVPTSRIQIEHTVKMSKEEIIEKSVECVEYIKSHGVKCMFSAMDATRTEVEYLKQIFKAVENA
KVDIVNVPDTVGVATPFKFYELIKQLREHLKVPIDVHCHNDFGLAVANTYAAVMAGANEVQVTVNGIGERAGNADLAQVV
MILHAIEGIKTNIKTEHLFETSKLVERLTGVKMPPNHPIVGENAFSHESGIHAHGVLKEYSTFEPGVVTPEMVGHKRRIV
IGKHAGRYQIKKILEEAGYHLDDEKLNQIFEKVKEMGDKGKRVTDLDLFAIAEVVIGELKREEKAILVDEVTVLTGNKIT
PTAVLNAEVFGHRKVTSAIGVGPVDASLKAVTSLVGESIRITEFRMDAITGGSDALAEVYVTVEDDEGHSFTSRGAAQDI
VMASIDAVINAVNYLLRMKRRKS
>Mature_502_residues
SAVMTTIRVFDTTLRDGEQMPGVSLPLEYKIRIAKQLDKLGVDVIEAGFPSATKGEFESVKEISSLSLNAEICGLARIVK
EDIDAAINANVDMVHIFVPTSRIQIEHTVKMSKEEIIEKSVECVEYIKSHGVKCMFSAMDATRTEVEYLKQIFKAVENAK
VDIVNVPDTVGVATPFKFYELIKQLREHLKVPIDVHCHNDFGLAVANTYAAVMAGANEVQVTVNGIGERAGNADLAQVVM
ILHAIEGIKTNIKTEHLFETSKLVERLTGVKMPPNHPIVGENAFSHESGIHAHGVLKEYSTFEPGVVTPEMVGHKRRIVI
GKHAGRYQIKKILEEAGYHLDDEKLNQIFEKVKEMGDKGKRVTDLDLFAIAEVVIGELKREEKAILVDEVTVLTGNKITP
TAVLNAEVFGHRKVTSAIGVGPVDASLKAVTSLVGESIRITEFRMDAITGGSDALAEVYVTVEDDEGHSFTSRGAAQDIV
MASIDAVINAVNYLLRMKRRKS

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786261, Length=500, Percent_Identity=36, Blast_Score=305, Evalue=4e-84,
Organism=Saccharomyces cerevisiae, GI6320019, Length=367, Percent_Identity=33.7874659400545, Blast_Score=204, Evalue=3e-53,
Organism=Saccharomyces cerevisiae, GI6320071, Length=355, Percent_Identity=34.0845070422535, Blast_Score=202, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6324682, Length=558, Percent_Identity=24.7311827956989, Blast_Score=130, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6324225, Length=557, Percent_Identity=23.8779174147217, Blast_Score=119, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24582381, Length=293, Percent_Identity=22.1843003412969, Blast_Score=69, Evalue=8e-12,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU12_ARCFU (O30020)

Other databases:

- EMBL:   AE000782
- PIR:   C69277
- RefSeq:   NP_069057.1
- ProteinModelPortal:   O30020
- GeneID:   1483430
- GenomeReviews:   AE000782_GR
- KEGG:   afu:AF0219
- NMPDR:   fig|224325.1.peg.216
- TIGR:   AF_0219
- HOGENOM:   HBG701974
- OMA:   ENSFAHE
- ProtClustDB:   PRK00915
- BioCyc:   AFUL224325:AF_0219-MONOMER
- BRENDA:   2.3.3.13
- HAMAP:   MF_01025_A
- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR011830
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00917
- TIGRFAMs:   TIGR02090

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer; SSF110921 2-isopropylmalate_synth_dimer

EC number: =2.3.3.13

Molecular weight: Translated: 55212; Mature: 55081

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1; PS00816 AIPM_HOMOCIT_SYNTH_2; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAVMTTIRVFDTTLRDGEQMPGVSLPLEYKIRIAKQLDKLGVDVIEAGFPSATKGEFES
CCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHCCCCCCCCCHHHH
VKEISSLSLNAEICGLARIVKEDIDAAINANVDMVHIFVPTSRIQIEHTVKMSKEEIIEK
HHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEEHHHHHCCHHHHHHH
SVECVEYIKSHGVKCMFSAMDATRTEVEYLKQIFKAVENAKVDIVNVPDTVGVATPFKFY
HHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHH
ELIKQLREHLKVPIDVHCHNDFGLAVANTYAAVMAGANEVQVTVNGIGERAGNADLAQVV
HHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHH
MILHAIEGIKTNIKTEHLFETSKLVERLTGVKMPPNHPIVGENAFSHESGIHAHGVLKEY
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
STFEPGVVTPEMVGHKRRIVIGKHAGRYQIKKILEEAGYHLDDEKLNQIFEKVKEMGDKG
CCCCCCCCCHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCC
KRVTDLDLFAIAEVVIGELKREEKAILVDEVTVLTGNKITPTAVLNAEVFGHRKVTSAIG
CEEEHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCCCEEEEHHHHCCHHHHHHCC
VGPVDASLKAVTSLVGESIRITEFRMDAITGGSDALAEVYVTVEDDEGHSFTSRGAAQDI
CCCCCHHHHHHHHHHCCCEEEEEEEEHHCCCCCCCEEEEEEEEECCCCCCCCCCCCHHHH
VMASIDAVINAVNYLLRMKRRKS
HHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SAVMTTIRVFDTTLRDGEQMPGVSLPLEYKIRIAKQLDKLGVDVIEAGFPSATKGEFES
CHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHCCCCCCCCCHHHH
VKEISSLSLNAEICGLARIVKEDIDAAINANVDMVHIFVPTSRIQIEHTVKMSKEEIIEK
HHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEEHHHHHCCHHHHHHH
SVECVEYIKSHGVKCMFSAMDATRTEVEYLKQIFKAVENAKVDIVNVPDTVGVATPFKFY
HHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHH
ELIKQLREHLKVPIDVHCHNDFGLAVANTYAAVMAGANEVQVTVNGIGERAGNADLAQVV
HHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHH
MILHAIEGIKTNIKTEHLFETSKLVERLTGVKMPPNHPIVGENAFSHESGIHAHGVLKEY
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
STFEPGVVTPEMVGHKRRIVIGKHAGRYQIKKILEEAGYHLDDEKLNQIFEKVKEMGDKG
CCCCCCCCCHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCC
KRVTDLDLFAIAEVVIGELKREEKAILVDEVTVLTGNKITPTAVLNAEVFGHRKVTSAIG
CEEEHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCCCEEEEHHHHCCHHHHHHCC
VGPVDASLKAVTSLVGESIRITEFRMDAITGGSDALAEVYVTVEDDEGHSFTSRGAAQDI
CCCCCHHHHHHHHHHCCCEEEEEEEEHHCCCCCCCEEEEEEEEECCCCCCCCCCCCHHHH
VMASIDAVINAVNYLLRMKRRKS
HHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475