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Identifier: 11497792

GI number: 11497792

Start: 153893

End: 154648

Strand: Reverse

Name: Not Available

Synonym: AF0175

Alternate gene names: 11497792

Gene position: 154648-153893 (Counterclockwise)

Preceding gene: 11497793

Following gene: 11497791

Centisome position: 7.1

GC content: 49.74

Gene sequence:

>756_bases
ATGGTGAGGTATGGAATGGTTATAGACCTGAACAGATGTATAGGCTGCAGAAGCTGCGCTGTTATTTGCAAAATTCACAA
CTCTCTCCCGCCGGGCACTTGGTGGCACAGAGTTGAGACCGTAGGGAGCAAAGAACATCAAACTCCGTCGGGAGATTACC
CCAATCTGACTGAACTTTACCTCCCAGTCCCCTGCATGCATTGCGATAATCCACCCTGCGTTAAGGTATGCCCCGTCGGT
GCAACGTGGAAAAGAGAGGACGGGGTTGTGCTGGTGGACTTTGAGAGGTGCATTGGCTGCAGGTACTGCATGACTGCCTG
CCCTTATGGTGTGAGGCAGTTTAACTGGGAAGATAAGGATAAGGCGGTAGAGAAAGGTTTCAAGGCGGCAGGATTTGAAG
GTGAGTACGTAGAAGGATACAGGCACGGATATCCCATTGATCACAGAACCAAAGACGGAAGGCTGGTTTACACGCCCAAG
AGACCAAGAGGAGTGGTTGAAAAGTGCACATTCTGTGTGCAGTATATTGACAAAGGTCTAAAGCCCGCATGTGTTAGAGG
CTGTCCAGGGAATGCGAGGATTTTCGGAGACCTCGATGACCCTAACAGTGAGATTTCAAAGGTGTTGAGGGACGGGGCAC
ACACGAGATTGCTGGAAGATTTGGGGACGGAGCCAAAGGTGTTCTACCTTTTACCTATGAAAAGGGCTGAAAGAGGAGAA
GTTAGGTATTCTCCAGAACTGGAGGTGAAATTGTGA

Upstream 100 bases:

>100_bases
AAACCAACCCATATTGTCTACTTCGTCCCCGGCATCTGGGAACCCACTACAGCGTGGAATGAGGTTGCGTGTGCAGTAAG
GAAGGTGGAGGTGTAGGTGC

Downstream 100 bases:

>100_bases
AGTTTAAAGCTTTAGTTGCGGTTCTGGTTGCCTTAGCAGCACTGGGAATGGCAGCATGGCTGTATCAGCTTCAGGAAGGA
TTGATAGTAACGAACATGCG

Product: molybdopterin oxidoreductase, iron-sulfur binding subunit

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: Hme subunit A [H]

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MVRYGMVIDLNRCIGCRSCAVICKIHNSLPPGTWWHRVETVGSKEHQTPSGDYPNLTELYLPVPCMHCDNPPCVKVCPVG
ATWKREDGVVLVDFERCIGCRYCMTACPYGVRQFNWEDKDKAVEKGFKAAGFEGEYVEGYRHGYPIDHRTKDGRLVYTPK
RPRGVVEKCTFCVQYIDKGLKPACVRGCPGNARIFGDLDDPNSEISKVLRDGAHTRLLEDLGTEPKVFYLLPMKRAERGE
VRYSPELEVKL

Sequences:

>Translated_251_residues
MVRYGMVIDLNRCIGCRSCAVICKIHNSLPPGTWWHRVETVGSKEHQTPSGDYPNLTELYLPVPCMHCDNPPCVKVCPVG
ATWKREDGVVLVDFERCIGCRYCMTACPYGVRQFNWEDKDKAVEKGFKAAGFEGEYVEGYRHGYPIDHRTKDGRLVYTPK
RPRGVVEKCTFCVQYIDKGLKPACVRGCPGNARIFGDLDDPNSEISKVLRDGAHTRLLEDLGTEPKVFYLLPMKRAERGE
VRYSPELEVKL
>Mature_251_residues
MVRYGMVIDLNRCIGCRSCAVICKIHNSLPPGTWWHRVETVGSKEHQTPSGDYPNLTELYLPVPCMHCDNPPCVKVCPVG
ATWKREDGVVLVDFERCIGCRYCMTACPYGVRQFNWEDKDKAVEKGFKAAGFEGEYVEGYRHGYPIDHRTKDGRLVYTPK
RPRGVVEKCTFCVQYIDKGLKPACVRGCPGNARIFGDLDDPNSEISKVLRDGAHTRLLEDLGTEPKVFYLLPMKRAERGE
VRYSPELEVKL

Specific function: Has menaquinol-oxidizing activity. HmeA, hmeB and hmeE subunits may together catalyze electron transfer from menaquinol to cytochrome c [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Extracellular side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Escherichia coli, GI2367345, Length=241, Percent_Identity=34.4398340248963, Blast_Score=128, Evalue=5e-31,
Organism=Escherichia coli, GI1787122, Length=200, Percent_Identity=33.5, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1787872, Length=200, Percent_Identity=33.5, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI226510944, Length=202, Percent_Identity=32.1782178217822, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1789370, Length=227, Percent_Identity=29.5154185022026, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787740, Length=149, Percent_Identity=32.2147651006711, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1787749, Length=203, Percent_Identity=28.0788177339901, Blast_Score=77, Evalue=8e-16,
Organism=Escherichia coli, GI1787478, Length=149, Percent_Identity=32.2147651006711, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1790326, Length=203, Percent_Identity=28.5714285714286, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87082114, Length=191, Percent_Identity=29.8429319371728, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1787963, Length=123, Percent_Identity=34.9593495934959, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI87082180, Length=114, Percent_Identity=35.9649122807018, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789067, Length=121, Percent_Identity=34.7107438016529, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI2367245, Length=121, Percent_Identity=33.8842975206612, Blast_Score=68, Evalue=5e-13,
Organism=Escherichia coli, GI87082179, Length=106, Percent_Identity=35.8490566037736, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788811, Length=115, Percent_Identity=31.304347826087, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 28465; Mature: 28465

Theoretical pI: Translated: 8.01; Mature: 8.01

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
8.4 %Cys+Met (Translated Protein)
6.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
8.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRYGMVIDLNRCIGCRSCAVICKIHNSLPPGTWWHRVETVGSKEHQTPSGDYPNLTELY
CCEEEEEEEHHHHCCHHHHHEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEE
LPVPCMHCDNPPCVKVCPVGATWKREDGVVLVDFERCIGCRYCMTACPYGVRQFNWEDKD
ECCCEEECCCCCEEEEECCCCCEECCCCEEEEEHHHHHCHHHHHHHCCCCHHCCCCCCHH
KAVEKGFKAAGFEGEYVEGYRHGYPIDHRTKDGRLVYTPKRPRGVVEKCTFCVQYIDKGL
HHHHCCCHHCCCCCHHHHHHHCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCC
KPACVRGCPGNARIFGDLDDPNSEISKVLRDGAHTRLLEDLGTEPKVFYLLPMKRAERGE
CCHHHCCCCCCCEEEECCCCCHHHHHHHHHCCHHHHHHHHCCCCCEEEEEEECCCCCCCC
VRYSPELEVKL
EEECCCEEEEC
>Mature Secondary Structure
MVRYGMVIDLNRCIGCRSCAVICKIHNSLPPGTWWHRVETVGSKEHQTPSGDYPNLTELY
CCEEEEEEEHHHHCCHHHHHEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEE
LPVPCMHCDNPPCVKVCPVGATWKREDGVVLVDFERCIGCRYCMTACPYGVRQFNWEDKD
ECCCEEECCCCCEEEEECCCCCEECCCCEEEEEHHHHHCHHHHHHHCCCCHHCCCCCCHH
KAVEKGFKAAGFEGEYVEGYRHGYPIDHRTKDGRLVYTPKRPRGVVEKCTFCVQYIDKGL
HHHHCCCHHCCCCCHHHHHHHCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCC
KPACVRGCPGNARIFGDLDDPNSEISKVLRDGAHTRLLEDLGTEPKVFYLLPMKRAERGE
CCHHHCCCCCCCEEEECCCCCHHHHHHHHHCCHHHHHHHHCCCCCEEEEEEECCCCCCCC
VRYSPELEVKL
EEECCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9389475; 11952791 [H]