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The map label for this gene is cysT

Identifier: 11497713

GI number: 11497713

Start: 90591

End: 91376

Strand: Reverse

Name: cysT

Synonym: AF0093

Alternate gene names: 11497713

Gene position: 91376-90591 (Counterclockwise)

Preceding gene: 11497714

Following gene: 11497712

Centisome position: 4.19

GC content: 50.76

Gene sequence:

>786_bases
ATGAGGCTGCTTTTCTCAGCCCTTCTGGCCCTTCTTTCTTCAATAATCCTCCTTTTCGTACTGCTGCCAGTTGCTGCCAC
CGTTACTCTCCAGCTCTTCAACTTTGACGAATTTTTGAAAGCTGCATCAGACCCGGCAGTTTGGAAAGTCGTCCTCACAA
CCTACTACGCAGCTTTGATTTCCACTCTAATCGCAGTAATTTTTGGCACACCGCTCGCCTACATTCTCGCAAGGAAGAGC
TTTCCGGGAAAAAGCGTTGTGGAGGGGATTGTTGATTTGCCCGTAGTTATTCCCCACACCGTGGCCGGAATAGCCCTGCT
TGTCGTTTTTGGGTCCAGCGGGCTGATTGGAAGCTTCTCCCCTCTCAAATTCGTCGATGCTCTTCCGGGAATCGTTGTTG
CAATGCTGTTCGTATCCGTGCCAATTTACATAAATCAGGCAAAGGAAGGCTTTGCCAGCGTTGACGTCAGGCTTGAGCAC
GTTGCAAGGACTCTTGGCTCCTCTCCGCTTAGGGTTTTCTTCACTGTCAGCCTCCCCCTCTCCGTAAGGCATATTGTGGC
AGGGGCAATAATGTCGTGGGCGAGGGGAATAAGCGAGTTTGGGGCTGTGGTGGTTATAGCATACTATCCCATGATTGCTC
CGACGCTGATTTACGAGCGCTACCTTTCTGAGGGCTTGTCGGCAGCCATGCCGGTTGCAGCAATCCTGATACTGCTCAGT
TTGGCAGTTTTTGTGGCCTTAAGAATTATTGTGGGGAGAGAAGATGTTTCTGAAGGTCAGGGCTGA

Upstream 100 bases:

>100_bases
AGGAGGGGCAGGAAATTCTAAGAGAGCTCGGGCAGGAGCCGCTTGTCCCACCAAGGGCCGACACAGCAGTTCCAAGCCTG
AAAGCAATGGTGGAGGTATC

Downstream 100 bases:

>100_bases
GAAAAGGCTGGGGAACTTCAGGCTTAACGTGGACTTTGAGATGGGCAGAGACTACTGCGTGCTGCTCGGCCCGACTGGTG
CTGGAAAGAGCGTTTTTCTC

Product: sulfate ABC transporter, permease protein (cysT)

Products: ADP; phosphate; MoO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MRLLFSALLALLSSIILLFVLLPVAATVTLQLFNFDEFLKAASDPAVWKVVLTTYYAALISTLIAVIFGTPLAYILARKS
FPGKSVVEGIVDLPVVIPHTVAGIALLVVFGSSGLIGSFSPLKFVDALPGIVVAMLFVSVPIYINQAKEGFASVDVRLEH
VARTLGSSPLRVFFTVSLPLSVRHIVAGAIMSWARGISEFGAVVVIAYYPMIAPTLIYERYLSEGLSAAMPVAAILILLS
LAVFVALRIIVGREDVSEGQG

Sequences:

>Translated_261_residues
MRLLFSALLALLSSIILLFVLLPVAATVTLQLFNFDEFLKAASDPAVWKVVLTTYYAALISTLIAVIFGTPLAYILARKS
FPGKSVVEGIVDLPVVIPHTVAGIALLVVFGSSGLIGSFSPLKFVDALPGIVVAMLFVSVPIYINQAKEGFASVDVRLEH
VARTLGSSPLRVFFTVSLPLSVRHIVAGAIMSWARGISEFGAVVVIAYYPMIAPTLIYERYLSEGLSAAMPVAAILILLS
LAVFVALRIIVGREDVSEGQG
>Mature_261_residues
MRLLFSALLALLSSIILLFVLLPVAATVTLQLFNFDEFLKAASDPAVWKVVLTTYYAALISTLIAVIFGTPLAYILARKS
FPGKSVVEGIVDLPVVIPHTVAGIALLVVFGSSGLIGSFSPLKFVDALPGIVVAMLFVSVPIYINQAKEGFASVDVRLEH
VARTLGSSPLRVFFTVSLPLSVRHIVAGAIMSWARGISEFGAVVVIAYYPMIAPTLIYERYLSEGLSAAMPVAAILILLS
LAVFVALRIIVGREDVSEGQG

Specific function: Part of the ABC transporter complex wtpABC involved in molybdate/tungstate import. Probably responsible for the translocation of the substrate across the membrane (Probable)

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain

Homologues:

Organism=Escherichia coli, GI1786980, Length=167, Percent_Identity=35.9281437125749, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1788764, Length=183, Percent_Identity=30.0546448087432, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI87082099, Length=172, Percent_Identity=29.6511627906977, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI1787080, Length=175, Percent_Identity=24.5714285714286, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): WTPB_ARCFU (O30143)

Other databases:

- EMBL:   AE000782
- PIR:   E69261
- RefSeq:   NP_068934.1
- PDB:   2ONK
- PDBsum:   2ONK
- ProteinModelPortal:   O30143
- SMR:   O30143
- GeneID:   1483305
- GenomeReviews:   AE000782_GR
- KEGG:   afu:AF0093
- NMPDR:   fig|224325.1.peg.93
- TIGR:   AF_0093
- HOGENOM:   HBG685300
- OMA:   NGLINGW
- BioCyc:   AFUL224325:AF_0093-MONOMER
- InterPro:   IPR000515

Pfam domain/function: PF00528 BPD_transp_1

EC number: NA

Molecular weight: Translated: 27834; Mature: 27834

Theoretical pI: Translated: 8.77; Mature: 8.77

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xc026c64)-; HASH(0xaf9bcfc)-; HASH(0xaf9bd80)-; HASH(0xaf8ad5c)-; HASH(0xaf925a0)-; HASH(0xaf88174)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLLFSALLALLSSIILLFVLLPVAATVTLQLFNFDEFLKAASDPAVWKVVLTTYYAALI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCHHHHHHHHHHHHHHHH
STLIAVIFGTPLAYILARKSFPGKSVVEGIVDLPVVIPHTVAGIALLVVFGSSGLIGSFS
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCC
PLKFVDALPGIVVAMLFVSVPIYINQAKEGFASVDVRLEHVARTLGSSPLRVFFTVSLPL
HHHHHHHHHHHHHHHHHHHHHHHEEHHHCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCH
SVRHIVAGAIMSWARGISEFGAVVVIAYYPMIAPTLIYERYLSEGLSAAMPVAAILILLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAVFVALRIIVGREDVSEGQG
HHHHHHHHHHHCCCHHCCCCH
>Mature Secondary Structure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HHHHHHHHHHHCCCHHCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; MoO42- [Periplasm]; H2O [C]

Specific reaction: ATP + MoO42- [Periplasm] + H2O = ADP + phosphate + MoO42- [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9389475