Click here to switch to the map view.

The map label for this gene is Not Available

Identifier: 11497649

GI number: 11497649

Start: 31091

End: 32206

Strand: Reverse

Name: Not Available

Synonym: AF0028

Alternate gene names: NA

Gene position: 32206-31091 (Counterclockwise)

Preceding gene: 11497650

Following gene: 11497646

Centisome position: 1.48

GC content: 50.99

Gene sequence:

>1116_bases
ATGTTCAAAAGGGAGACAAAGGACTTCATAAACATCGACCCTTTGCAGACTGGCGGAAAGCTCACCGAGGAGGCGAGGCA
GGCTCTGCTTGAGTGGGGGGATGGCTACAGCGTCTGCGACTTCTGCACCACAGGAAGGCTAGACGAGATAAAGACTCCCC
CCATTCACGACTTCATCCACAATCAGCTTCCAAAATTCTTGGGCTGTGATGTGGCGAGAGTAACAAACGGGGCGAGGGAG
GCGAAGTTTGCAGTGATGCACTCCCTCGCCAAAAAAGATGCCTGGGTTGTCATGGATGAGAACTGCCACTACTCCAGCTA
CGTTGCTGCTGAGAGGGCCGGGCTCAACATTGCGCTGGTTCCGAAAACTGACTACCCCGACTACGCCATTACCCCCGAAA
ACTTCGCCCAAACAATCGAGGAGACCAAGAAAAGGGGTGAGGTTGTCCTTGCCCTCATAACCTACCCCGATGGCAACTAC
GGCAACCTTCCCGATGTGAAGAAAATTGCAAAAGTTTGCTCCGAGTATGACGTTCCCTTACTCGTTAACGGAGCATATGC
CATCGGAAGAATGCCAGTAAGCCTGAAGGAAATCGGTGCTGACTTCATAGTCGGCAGCGGGCACAAGAGCATGGCCGCAA
GCGGACCCATAGGTGTGATGGGAATGAAGGAGGAGTGGGCTGAAATTGTGCTGAGAAGATCCGAGAAGTACAAAAACAAG
GAGGTTGAGCTCCTCGGCTGCACCGCAAGGGGTGCGACAATCATAACGCTGATGGCATCCTTCCCGCATGTGAGGGAGAG
GATTAAGAGGTGGGATGAGGAGGTAGAGAAGGCCAGGAGGTTCGCTGCTGAAATGGAAAAACTCGGAATAAAGCAGCTCG
GCGATAATCCTCACAACCACGATTTGATGTTCTTCCATGCTGAGGTTCTCTACGAGATTTCCAAAAAAGCAAAGGGGGGA
AGGTTCTTCCTTTACAGGGAGCTCAAGTCGAGAAAAATACACGGAATAAAGCCCGGCCTAACAAGGTACTTCAAGCTCTC
CACATACGGGCTGAGCGATGAAGAGGTGGATTACGTGCTCAATGCTTTTAAAGAAATAATAGAAAAATACTCATGA

Upstream 100 bases:

>100_bases
GTAAAGCGCTACAACAAACTGCTCAAAGCCGGTAGGAGAGTTGTTCTCGCAGCTCATCTGACATGCTAAACTTTTTTAAG
TTCTGCATCTGCCTTAAGGC

Downstream 100 bases:

>100_bases
CTTCAATTTTTCCAGAAATTCATCCACGGGTATGGCTGGCATTGTTACCGTCCTTATTGTCCCTCTGCTGGCCAGCTCGA
CCATCGCCTTCATAACAACC

Product: Sep-tRNA:Cys-tRNA synthetase

Products: NA

Alternate protein names: Sep-tRNA:Cys-tRNA synthase 1; SepCysS 1

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MFKRETKDFINIDPLQTGGKLTEEARQALLEWGDGYSVCDFCTTGRLDEIKTPPIHDFIHNQLPKFLGCDVARVTNGARE
AKFAVMHSLAKKDAWVVMDENCHYSSYVAAERAGLNIALVPKTDYPDYAITPENFAQTIEETKKRGEVVLALITYPDGNY
GNLPDVKKIAKVCSEYDVPLLVNGAYAIGRMPVSLKEIGADFIVGSGHKSMAASGPIGVMGMKEEWAEIVLRRSEKYKNK
EVELLGCTARGATIITLMASFPHVRERIKRWDEEVEKARRFAAEMEKLGIKQLGDNPHNHDLMFFHAEVLYEISKKAKGG
RFFLYRELKSRKIHGIKPGLTRYFKLSTYGLSDEEVDYVLNAFKEIIEKYS

Sequences:

>Translated_371_residues
MFKRETKDFINIDPLQTGGKLTEEARQALLEWGDGYSVCDFCTTGRLDEIKTPPIHDFIHNQLPKFLGCDVARVTNGARE
AKFAVMHSLAKKDAWVVMDENCHYSSYVAAERAGLNIALVPKTDYPDYAITPENFAQTIEETKKRGEVVLALITYPDGNY
GNLPDVKKIAKVCSEYDVPLLVNGAYAIGRMPVSLKEIGADFIVGSGHKSMAASGPIGVMGMKEEWAEIVLRRSEKYKNK
EVELLGCTARGATIITLMASFPHVRERIKRWDEEVEKARRFAAEMEKLGIKQLGDNPHNHDLMFFHAEVLYEISKKAKGG
RFFLYRELKSRKIHGIKPGLTRYFKLSTYGLSDEEVDYVLNAFKEIIEKYS
>Mature_371_residues
MFKRETKDFINIDPLQTGGKLTEEARQALLEWGDGYSVCDFCTTGRLDEIKTPPIHDFIHNQLPKFLGCDVARVTNGARE
AKFAVMHSLAKKDAWVVMDENCHYSSYVAAERAGLNIALVPKTDYPDYAITPENFAQTIEETKKRGEVVLALITYPDGNY
GNLPDVKKIAKVCSEYDVPLLVNGAYAIGRMPVSLKEIGADFIVGSGHKSMAASGPIGVMGMKEEWAEIVLRRSEKYKNK
EVELLGCTARGATIITLMASFPHVRERIKRWDEEVEKARRFAAEMEKLGIKQLGDNPHNHDLMFFHAEVLYEISKKAKGG
RFFLYRELKSRKIHGIKPGLTRYFKLSTYGLSDEEVDYVLNAFKEIIEKYS

Specific function: Converts O-phospho-L-seryl-tRNA(Cys) (Sep-tRNA(Cys)) to L-cysteinyl-tRNA(Cys) (Cys-tRNA(Cys))

COG id: COG1103

COG function: function code R; Archaea-specific pyridoxal phosphate-dependent enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SepCysS family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SPSS1_ARCFU (O30207)

Other databases:

- EMBL:   AE000782
- PIR:   D69253
- RefSeq:   NP_068869.1
- PDB:   2E7I
- PDB:   2E7J
- PDBsum:   2E7I
- PDBsum:   2E7J
- ProteinModelPortal:   O30207
- SMR:   O30207
- GeneID:   1483238
- GenomeReviews:   AE000782_GR
- KEGG:   afu:AF0028
- NMPDR:   fig|224325.1.peg.28
- TIGR:   AF_0028
- HOGENOM:   HBG539014
- OMA:   HKSMAAS
- ProtClustDB:   PRK09331
- BioCyc:   AFUL224325:AF_0028-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_01675
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR013375
- InterPro:   IPR008829
- Gene3D:   G3DSA:3.40.640.10
- TIGRFAMs:   TIGR02539

Pfam domain/function: PF05889 SLA_LP_auto_ag; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.5.1.73

Molecular weight: Translated: 41931; Mature: 41931

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: NA

Important sites: BINDING 79-79 BINDING 103-103 BINDING 104-104 BINDING 183-183 BINDING 267-267 BINDING 270-270

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKRETKDFINIDPLQTGGKLTEEARQALLEWGDGYSVCDFCTTGRLDEIKTPPIHDFIH
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCHHHHHH
NQLPKFLGCDVARVTNGAREAKFAVMHSLAKKDAWVVMDENCHYSSYVAAERAGLNIALV
HHCHHHHCCCHHHHCCCHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHCCCEEEEE
PKTDYPDYAITPENFAQTIEETKKRGEVVLALITYPDGNYGNLPDVKKIAKVCSEYDVPL
ECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHCCCCCE
LVNGAYAIGRMPVSLKEIGADFIVGSGHKSMAASGPIGVMGMKEEWAEIVLRRSEKYKNK
EEECCHHCCCCCCCHHHCCCCEEEECCCCCCCCCCCEEECCCHHHHHHHHHHHHHHCCCC
EVELLGCTARGATIITLMASFPHVRERIKRWDEEVEKARRFAAEMEKLGIKQLGDNPHNH
CEEEEEECCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
DLMFFHAEVLYEISKKAKGGRFFLYRELKSRKIHGIKPGLTRYFKLSTYGLSDEEVDYVL
CEEEEHHHHHHHHHHHCCCCEEEEEEEHHCCCCCCCCCCHHHEEEEEECCCCHHHHHHHH
NAFKEIIEKYS
HHHHHHHHHCC
>Mature Secondary Structure
MFKRETKDFINIDPLQTGGKLTEEARQALLEWGDGYSVCDFCTTGRLDEIKTPPIHDFIH
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCHHHHHH
NQLPKFLGCDVARVTNGAREAKFAVMHSLAKKDAWVVMDENCHYSSYVAAERAGLNIALV
HHCHHHHCCCHHHHCCCHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHCCCEEEEE
PKTDYPDYAITPENFAQTIEETKKRGEVVLALITYPDGNYGNLPDVKKIAKVCSEYDVPL
ECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHCCCCCE
LVNGAYAIGRMPVSLKEIGADFIVGSGHKSMAASGPIGVMGMKEEWAEIVLRRSEKYKNK
EEECCHHCCCCCCCHHHCCCCEEEECCCCCCCCCCCEEECCCHHHHHHHHHHHHHHCCCC
EVELLGCTARGATIITLMASFPHVRERIKRWDEEVEKARRFAAEMEKLGIKQLGDNPHNH
CEEEEEECCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
DLMFFHAEVLYEISKKAKGGRFFLYRELKSRKIHGIKPGLTRYFKLSTYGLSDEEVDYVL
CEEEEHHHHHHHHHHHCCCCEEEEEEEHHCCCCCCCCCCHHHEEEEEECCCCHHHHHHHH
NAFKEIIEKYS
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475