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Identifier: 11497645

GI number: 11497645

Start: 27284

End: 28534

Strand: Reverse

Name: Not Available

Synonym: AF0024

Alternate gene names: 11497645

Gene position: 28534-27284 (Counterclockwise)

Preceding gene: 11497646

Following gene: 11497644

Centisome position: 1.31

GC content: 53.08

Gene sequence:

>1251_bases
GTGTTGTGTATGGCTGAATGGCCGATGGACAGACCTGAGTTGAGAACCATCATAACGACCTACAGCGTTCCAGAGTCGAA
GCTTCTGCTGAACTCATTCATCATGCCGAAGACCTTCTACGGGTTGGCCGGTTTGCAGATGTTTGCTGGAGCCATGGTGC
AGTTCGGAAGAAGGAGGGCTTTCATAGTCACCGATAAGGGAGTGAGAGCCCTTGCTCTGACAGTGCAAAAAATGCTTAAG
GGTGTCGGCATTGAAGTCGAGATTTTCGACGAAGCGAAGCCTGAGGTGCCGTTGAGCTCGATAAAGCCCGCTGCAGAAAA
AATGGAAGCCCTCCAGCCCGACTTGATTGTGGCAGTTGGTGGTGGAAGCGTTATGGACATGGCGAAGGCCTCGTGGATTC
TCTACGAGGCTCCGCAGACAGACCTGACAAACGTCGATGCGATGCAGCCGATCGGGCTGAGGAGAAAGGCGTTTCTGGCG
TGCATCCCAACAACTGCCGGAACGGGAAGCGAGGCAACAAACGTTGCGGTCATTACAGATGACACTGTGGAGCCGCACAG
GAAAGAGGGTGTTGTGCATCCTGAGCTTTATCCTGACTTCGCCGTCCTCGACCCGCGATTTGTTTTGAAAATGCCTCCGA
ATCTCACACTTGGGACGGGGCTGGATGCCCTGTCGCATGCAGTTGACTGCTACCTCTGCAGGTCGAGCAACGAAATTTCC
GACGCTCTGGCGGTGAGAGCGACAAAGCTCGTTTTCAAGTACCTCCCGTTAGCCCTGAAGTACCCCGACAACCCGCAGAT
CAGGCTCAAAACTCAGATTGCCGCCATGATTGCAGGTTCAGCCTTTTCGAACGGCGGAATAGGAACGACCCACGCAGTTG
CGCACAACATAGGCGCGCTCTTCGAGGTTCACCACGGTATGGCGTGTGGGGTTACAATACCCTACGTCATCAAGTTCTAC
GCGGAGGTGGATGATAAGTACCTCAGCTTTGCCGAGGAGCTTGGCGTGAGAGGAGGCAGCAACGAAGAAACGCTGAAAAA
GCTCATAGACATTTTCATGGACTTCTACAGGAAAATAGGGGCACCAACGACTATGAGAGACCTTGGCATAAAAGAGGATG
ACTTCAAGGAGAAGCTCGACACCTTCGCACAGTACGTTGTTAAAGACCCAACAGGGCCCTTCGCTCCGAGGCAGCTTACT
GTTGAGGAGGCGAAGGAGTTGTTGATGGATATGTATTATGGGAGGTTGTGA

Upstream 100 bases:

>100_bases
GCAAGCTCTGGCTTCACCACCGCCTTCACCGTAGCCGCGATATTCTTCGCCGCTCAGGGCATATTTGCGGTCCTTATTCC
GGAACCGCCCAAATAAGGAG

Downstream 100 bases:

>100_bases
GTATGATGGGATTTCATGGTAAAATTCTGGAAGTGAACCTTTCAAACTCGACCGTTAGCGAGATAGAGGTTACAGAGGAG
ATGATAAAGAAGTTCGTCGG

Product: alcohol dehydrogenase, iron-containing

Products: aldehyde; ketone; NADH; H+

Alternate protein names: BDH [H]

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MLCMAEWPMDRPELRTIITTYSVPESKLLLNSFIMPKTFYGLAGLQMFAGAMVQFGRRRAFIVTDKGVRALALTVQKMLK
GVGIEVEIFDEAKPEVPLSSIKPAAEKMEALQPDLIVAVGGGSVMDMAKASWILYEAPQTDLTNVDAMQPIGLRRKAFLA
CIPTTAGTGSEATNVAVITDDTVEPHRKEGVVHPELYPDFAVLDPRFVLKMPPNLTLGTGLDALSHAVDCYLCRSSNEIS
DALAVRATKLVFKYLPLALKYPDNPQIRLKTQIAAMIAGSAFSNGGIGTTHAVAHNIGALFEVHHGMACGVTIPYVIKFY
AEVDDKYLSFAEELGVRGGSNEETLKKLIDIFMDFYRKIGAPTTMRDLGIKEDDFKEKLDTFAQYVVKDPTGPFAPRQLT
VEEAKELLMDMYYGRL

Sequences:

>Translated_416_residues
MLCMAEWPMDRPELRTIITTYSVPESKLLLNSFIMPKTFYGLAGLQMFAGAMVQFGRRRAFIVTDKGVRALALTVQKMLK
GVGIEVEIFDEAKPEVPLSSIKPAAEKMEALQPDLIVAVGGGSVMDMAKASWILYEAPQTDLTNVDAMQPIGLRRKAFLA
CIPTTAGTGSEATNVAVITDDTVEPHRKEGVVHPELYPDFAVLDPRFVLKMPPNLTLGTGLDALSHAVDCYLCRSSNEIS
DALAVRATKLVFKYLPLALKYPDNPQIRLKTQIAAMIAGSAFSNGGIGTTHAVAHNIGALFEVHHGMACGVTIPYVIKFY
AEVDDKYLSFAEELGVRGGSNEETLKKLIDIFMDFYRKIGAPTTMRDLGIKEDDFKEKLDTFAQYVVKDPTGPFAPRQLT
VEEAKELLMDMYYGRL
>Mature_416_residues
MLCMAEWPMDRPELRTIITTYSVPESKLLLNSFIMPKTFYGLAGLQMFAGAMVQFGRRRAFIVTDKGVRALALTVQKMLK
GVGIEVEIFDEAKPEVPLSSIKPAAEKMEALQPDLIVAVGGGSVMDMAKASWILYEAPQTDLTNVDAMQPIGLRRKAFLA
CIPTTAGTGSEATNVAVITDDTVEPHRKEGVVHPELYPDFAVLDPRFVLKMPPNLTLGTGLDALSHAVDCYLCRSSNEIS
DALAVRATKLVFKYLPLALKYPDNPQIRLKTQIAAMIAGSAFSNGGIGTTHAVAHNIGALFEVHHGMACGVTIPYVIKFY
AEVDDKYLSFAEELGVRGGSNEETLKKLIDIFMDFYRKIGAPTTMRDLGIKEDDFKEKLDTFAQYVVKDPTGPFAPRQLT
VEEAKELLMDMYYGRL

Specific function: This enzyme has activity using butanol and ethanol as substrates [H]

COG id: COG1454

COG function: function code C; Alcohol dehydrogenase, class IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI133922590, Length=337, Percent_Identity=28.7833827893175, Blast_Score=108, Evalue=8e-24,
Organism=Escherichia coli, GI1787493, Length=413, Percent_Identity=32.6876513317191, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI48994951, Length=361, Percent_Identity=31.5789473684211, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1789163, Length=387, Percent_Identity=29.7157622739018, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI87082107, Length=391, Percent_Identity=28.1329923273657, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1789386, Length=302, Percent_Identity=26.8211920529801, Blast_Score=92, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17537053, Length=367, Percent_Identity=28.0653950953678, Blast_Score=130, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6321181, Length=384, Percent_Identity=29.1666666666667, Blast_Score=176, Evalue=7e-45,
Organism=Drosophila melanogaster, GI24657991, Length=406, Percent_Identity=27.3399014778325, Blast_Score=107, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: 1.1.1.1

Molecular weight: Translated: 45785; Mature: 45785

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS00060 ADH_IRON_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLCMAEWPMDRPELRTIITTYSVPESKLLLNSFIMPKTFYGLAGLQMFAGAMVQFGRRRA
CEECCCCCCCCHHHHEEEEECCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCEE
FIVTDKGVRALALTVQKMLKGVGIEVEIFDEAKPEVPLSSIKPAAEKMEALQPDLIVAVG
EEEECCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHCCHHHHHHHHCCCCEEEEEC
GGSVMDMAKASWILYEAPQTDLTNVDAMQPIGLRRKAFLACIPTTAGTGSEATNVAVITD
CCCHHHHHHHCEEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCEEEEEEC
DTVEPHRKEGVVHPELYPDFAVLDPRFVLKMPPNLTLGTGLDALSHAVDCYLCRSSNEIS
CCCCCHHHCCCCCCCCCCCCEEECCEEEEECCCCCEECCCHHHHHHHHHEEEECCCCCHH
DALAVRATKLVFKYLPLALKYPDNPQIRLKTQIAAMIAGSAFSNGGIGTTHAVAHNIGAL
HHHHHHHHHHHHHHHCCEEECCCCCCEEEHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
FEVHHGMACGVTIPYVIKFYAEVDDKYLSFAEELGVRGGSNEETLKKLIDIFMDFYRKIG
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHC
APTTMRDLGIKEDDFKEKLDTFAQYVVKDPTGPFAPRQLTVEEAKELLMDMYYGRL
CCCCHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEECHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLCMAEWPMDRPELRTIITTYSVPESKLLLNSFIMPKTFYGLAGLQMFAGAMVQFGRRRA
CEECCCCCCCCHHHHEEEEECCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCEE
FIVTDKGVRALALTVQKMLKGVGIEVEIFDEAKPEVPLSSIKPAAEKMEALQPDLIVAVG
EEEECCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHCCHHHHHHHHCCCCEEEEEC
GGSVMDMAKASWILYEAPQTDLTNVDAMQPIGLRRKAFLACIPTTAGTGSEATNVAVITD
CCCHHHHHHHCEEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCEEEEEEC
DTVEPHRKEGVVHPELYPDFAVLDPRFVLKMPPNLTLGTGLDALSHAVDCYLCRSSNEIS
CCCCCHHHCCCCCCCCCCCCEEECCEEEEECCCCCEECCCHHHHHHHHHEEEECCCCCHH
DALAVRATKLVFKYLPLALKYPDNPQIRLKTQIAAMIAGSAFSNGGIGTTHAVAHNIGAL
HHHHHHHHHHHHHHHCCEEECCCCCCEEEHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
FEVHHGMACGVTIPYVIKFYAEVDDKYLSFAEELGVRGGSNEETLKKLIDIFMDFYRKIG
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHC
APTTMRDLGIKEDDFKEKLDTFAQYVVKDPTGPFAPRQLTVEEAKELLMDMYYGRL
CCCCHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEECHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 1000 [C]

Specific activity: NA

Km value (mM): 0.15 {ethanol}} 7.05 {acetaldehyde}} 0.1 {acetaldehyde}} [C]

Substrates: alcohol; NAD+

Specific reaction: an alcohol + NAD+ = an aldehyde or ketone + NADH + H+

General reaction: Redox reaction [C]

Inhibitor: 8-Hydroxy quinoline-5-sulfonic acid; Dipicolinic acid; EDTA; Ethanol; GSH; Heptafluorbutanol; Imidazole; Iodoacetamide; Iodoacetate; Isobutyramide EDTA; NAD+; NADH; NEM; o-Phenanthroline of Zn2+; PCMB; Pyrazole; Substituted pyrazoles 4-iodo-; Zn2+ [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2673928 [H]