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The map label for this gene is pyrE

Identifier: 15679848

GI number: 15679848

Start: 1697314

End: 1697856

Strand: Direct

Name: pyrE

Synonym: MTH1860

Alternate gene names: 15679848

Gene position: 1697314-1697856 (Clockwise)

Preceding gene: 15679847

Following gene: 15679850

Centisome position: 96.91

GC content: 49.54

Gene sequence:

>543_bases
ATGCAGGTCAAAAATACAGAGGAACTCAGAAGGGAGCTTATAGAACTTCTGTCTGAAATGGATGTCGTTCAGAGGGGTAA
ATTCATCCTCTCATCAGGCAGGGAAAGCGATTACTACGTTGATATAAAGAGGGCGGTAACGGAGCCGGCTGTCCTTGACG
TTATAGCGAGGTTAATAGCCGATGCGGCCGGTGAAGTGGACAGGATAGCAGGTCCTGCCCTTGGAGCGGTCCCAATAGCC
ACTGCGGTGTCACTCTACTCCAGAAAACCGCTCCTGATGATAAGAAAGGAGAAAAAGGGATACGGAACATCAAAACTCAT
TGAGGGGGATCTGCAGAAAGGTGACAGGGTTGCCGTGGTTGAGGACGTTACAACGACTGGCGGGTCTCTTCTTAAGGCTG
TTAGGGCAATTCAGGAGAACGGTGGGATCGTTGAGAAGGCATTTGTTATTGTTGACCGTGAGGAAGGGGCTGTAGACGAA
TTTAAAAGGGAGGGCATAACCCTCATACCCCTCCTTTCGGTATCTGACTTCAACCATTCCTGA

Upstream 100 bases:

>100_bases
GCAGCATCTGCGGGCGTGCAGGTTTTCTTCACACGTGTAGACTTTGCGGGGCACAGGTATGTTCAGATTGCTACGTTCCA
GAGCTGGGAGTCTGTAAGAT

Downstream 100 bases:

>100_bases
ACATTCTCTTATTATTTTTTTCACTATTCCCGCAGGTGCTTCACAAGGTGACCGATCTCCGGGACCACAATTTCGATTCC
AAGCTTAACCGCGTTGGGCG

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase

Number of amino acids: Translated: 180; Mature: 180

Protein sequence:

>180_residues
MQVKNTEELRRELIELLSEMDVVQRGKFILSSGRESDYYVDIKRAVTEPAVLDVIARLIADAAGEVDRIAGPALGAVPIA
TAVSLYSRKPLLMIRKEKKGYGTSKLIEGDLQKGDRVAVVEDVTTTGGSLLKAVRAIQENGGIVEKAFVIVDREEGAVDE
FKREGITLIPLLSVSDFNHS

Sequences:

>Translated_180_residues
MQVKNTEELRRELIELLSEMDVVQRGKFILSSGRESDYYVDIKRAVTEPAVLDVIARLIADAAGEVDRIAGPALGAVPIA
TAVSLYSRKPLLMIRKEKKGYGTSKLIEGDLQKGDRVAVVEDVTTTGGSLLKAVRAIQENGGIVEKAFVIVDREEGAVDE
FKREGITLIPLLSVSDFNHS
>Mature_180_residues
MQVKNTEELRRELIELLSEMDVVQRGKFILSSGRESDYYVDIKRAVTEPAVLDVIARLIADAAGEVDRIAGPALGAVPIA
TAVSLYSRKPLLMIRKEKKGYGTSKLIEGDLQKGDRVAVVEDVTTTGGSLLKAVRAIQENGGIVEKAFVIVDREEGAVDE
FKREGITLIPLLSVSDFNHS

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP)

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily

Homologues:

Organism=Homo sapiens, GI4507835, Length=167, Percent_Identity=33.5329341317365, Blast_Score=106, Evalue=1e-23,
Organism=Escherichia coli, GI1790073, Length=183, Percent_Identity=30.6010928961749, Blast_Score=70, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17554964, Length=171, Percent_Identity=37.4269005847953, Blast_Score=111, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17508631, Length=167, Percent_Identity=33.5329341317365, Blast_Score=104, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6323530, Length=196, Percent_Identity=26.530612244898, Blast_Score=64, Evalue=2e-11,
Organism=Drosophila melanogaster, GI17933654, Length=179, Percent_Identity=35.195530726257, Blast_Score=97, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYRE_METTH (O27888)

Other databases:

- EMBL:   AE000666
- PIR:   H69115
- RefSeq:   NP_276966.1
- ProteinModelPortal:   O27888
- SMR:   O27888
- STRING:   O27888
- GeneID:   1470945
- GenomeReviews:   AE000666_GR
- KEGG:   mth:MTH1860
- NMPDR:   fig|187420.1.peg.1821
- eggNOG:   arNOG09150
- HOGENOM:   HBG404341
- OMA:   GKEADYY
- PhylomeDB:   O27888
- ProtClustDB:   PRK00455
- BioCyc:   MTHE187420:MTH1860-MONOMER
- BRENDA:   2.4.2.10
- HAMAP:   MF_01208
- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836
- TIGRFAMs:   TIGR00336

Pfam domain/function: PF00156 Pribosyltran

EC number: =2.4.2.10

Molecular weight: Translated: 19639; Mature: 19639

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: BINDING 95-95 BINDING 96-96 BINDING 99-99 BINDING 125-125 BINDING 153-153

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVKNTEELRRELIELLSEMDVVQRGKFILSSGRESDYYVDIKRAVTEPAVLDVIARLIA
CCCCCHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCEEEEHHHHHCCHHHHHHHHHHHH
DAAGEVDRIAGPALGAVPIATAVSLYSRKPLLMIRKEKKGYGTSKLIEGDLQKGDRVAVV
HHCCCHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHCCCCCEEEEE
EDVTTTGGSLLKAVRAIQENGGIVEKAFVIVDREEGAVDEFKREGITLIPLLSVSDFNHS
ECCCCCCHHHHHHHHHHHHCCCEEEEEEEEEECCCCCHHHHHHCCCEEEEEEEECCCCCC
>Mature Secondary Structure
MQVKNTEELRRELIELLSEMDVVQRGKFILSSGRESDYYVDIKRAVTEPAVLDVIARLIA
CCCCCHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCEEEEHHHHHCCHHHHHHHHHHHH
DAAGEVDRIAGPALGAVPIATAVSLYSRKPLLMIRKEKKGYGTSKLIEGDLQKGDRVAVV
HHCCCHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHCCCCCEEEEE
EDVTTTGGSLLKAVRAIQENGGIVEKAFVIVDREEGAVDEFKREGITLIPLLSVSDFNHS
ECCCCCCHHHHHHHHHHHHCCCEEEEEEEEEECCCCCHHHHHHCCCEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463