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Identifier: 15679708

GI number: 15679708

Start: 1571377

End: 1571859

Strand: Direct

Name: Not Available

Synonym: MTH1714

Alternate gene names: 15679708

Gene position: 1571377-1571859 (Clockwise)

Preceding gene: 15679707

Following gene: 15679714

Centisome position: 89.72

GC content: 54.45

Gene sequence:

>483_bases
GTGAGGGGTGGTTCAGTGAAGATGCTGATGGTCATGGACCCTGCGAGGTGCAGTGGCTGCGATGACTGTATAAATGCGTG
CAGGGAAACCCATGGAGTCGCCAGGGCAAGGAAGGGAGATAGGATGCCCCTATTCTGTCTGCAGTGCCATCCAGAGAAGG
CTCCCTGCGCAAGGATATGTCCGGTGGGTGCCATAAGGGAGGTTGACGGGGCCCTTGTTGTTGATGAGGAGTCCTGCATA
CTCTGCAAGCTATGCATGGTGGCCTGCCCGGCTGGCATGATTGTGATGAACACTGAGAAGAAATCAGCAGAGAAATGCAC
ACTCTGCATGGACGCAGAAGTCATACTGCCAGCCTGTGTGGAGGCATGCAAGGAGAACGTTCTTAAACTGGTCTCCCTGG
ATGACCTTGCTGAGCTGAGGGATCGCGCTGACTTCAGTGAGGTCCTTGAGGAGGCGGTGAAGATATACGGGAAAAAGATC
TAG

Upstream 100 bases:

>100_bases
CTGCAAGCGGTGAGATAGAGGATGATACTGGCTGGAGAGTCCTTGTTGGACCAAGGGACTCCTCTGGAATACCAGACTAC
CTTGATAAACTTGCATCAGA

Downstream 100 bases:

>100_bases
CCATCCCCTCTCTCCCCGTAGCCTTGGATTTCAACGTGAAGGGTGTTTATGAACACCCTTATATATTTATATGATTCCTT
CAGGTCCTCAATGGGGCCCC

Product: formate hydrogenlyase, iron-sulfur subunit 2

Products: CO2; NADH

Alternate protein names: NA

Number of amino acids: Translated: 160; Mature: 160

Protein sequence:

>160_residues
MRGGSVKMLMVMDPARCSGCDDCINACRETHGVARARKGDRMPLFCLQCHPEKAPCARICPVGAIREVDGALVVDEESCI
LCKLCMVACPAGMIVMNTEKKSAEKCTLCMDAEVILPACVEACKENVLKLVSLDDLAELRDRADFSEVLEEAVKIYGKKI

Sequences:

>Translated_160_residues
MRGGSVKMLMVMDPARCSGCDDCINACRETHGVARARKGDRMPLFCLQCHPEKAPCARICPVGAIREVDGALVVDEESCI
LCKLCMVACPAGMIVMNTEKKSAEKCTLCMDAEVILPACVEACKENVLKLVSLDDLAELRDRADFSEVLEEAVKIYGKKI
>Mature_160_residues
MRGGSVKMLMVMDPARCSGCDDCINACRETHGVARARKGDRMPLFCLQCHPEKAPCARICPVGAIREVDGALVVDEESCI
LCKLCMVACPAGMIVMNTEKKSAEKCTLCMDAEVILPACVEACKENVLKLVSLDDLAELRDRADFSEVLEEAVKIYGKKI

Specific function: Unknown

COG id: COG1142

COG function: function code C; Fe-S-cluster-containing hydrogenase components 2

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI226510944, Length=174, Percent_Identity=30.4597701149425, Blast_Score=76, Evalue=9e-16,
Organism=Escherichia coli, GI2367345, Length=151, Percent_Identity=33.7748344370861, Blast_Score=74, Evalue=5e-15,
Organism=Escherichia coli, GI2367245, Length=151, Percent_Identity=36.4238410596026, Blast_Score=73, Evalue=9e-15,
Organism=Escherichia coli, GI1788811, Length=144, Percent_Identity=36.1111111111111, Blast_Score=72, Evalue=1e-14,
Organism=Escherichia coli, GI87082114, Length=174, Percent_Identity=28.1609195402299, Blast_Score=72, Evalue=1e-14,
Organism=Escherichia coli, GI87082179, Length=155, Percent_Identity=32.258064516129, Blast_Score=71, Evalue=3e-14,
Organism=Escherichia coli, GI1789079, Length=178, Percent_Identity=33.1460674157303, Blast_Score=69, Evalue=1e-13,
Organism=Escherichia coli, GI1787122, Length=159, Percent_Identity=30.8176100628931, Blast_Score=68, Evalue=3e-13,
Organism=Escherichia coli, GI1787872, Length=165, Percent_Identity=29.6969696969697, Blast_Score=66, Evalue=1e-12,
Organism=Escherichia coli, GI1787963, Length=159, Percent_Identity=30.188679245283, Blast_Score=64, Evalue=6e-12,
Organism=Escherichia coli, GI1789067, Length=163, Percent_Identity=32.5153374233129, Blast_Score=60, Evalue=7e-11,
Organism=Escherichia coli, GI87082180, Length=149, Percent_Identity=28.8590604026846, Blast_Score=60, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR000813 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: 1.2.1.2

Molecular weight: Translated: 17429; Mature: 17429

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

10.0 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
15.6 %Cys+Met (Translated Protein)
10.0 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
15.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGGSVKMLMVMDPARCSGCDDCINACRETHGVARARKGDRMPLFCLQCHPEKAPCARIC
CCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHC
PVGAIREVDGALVVDEESCILCKLCMVACPAGMIVMNTEKKSAEKCTLCMDAEVILPACV
CCCCHHHCCCEEEECCCCHHHHHHHHHHCCCCEEEEECCCCCHHHEEECCCHHHHHHHHH
EACKENVLKLVSLDDLAELRDRADFSEVLEEAVKIYGKKI
HHHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRGGSVKMLMVMDPARCSGCDDCINACRETHGVARARKGDRMPLFCLQCHPEKAPCARIC
CCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHC
PVGAIREVDGALVVDEESCILCKLCMVACPAGMIVMNTEKKSAEKCTLCMDAEVILPACV
CCCCHHHCCCEEEECCCCHHHHHHHHHHCCCCEEEEECCCCCHHHEEECCCHHHHHHHHH
EACKENVLKLVSLDDLAELRDRADFSEVLEEAVKIYGKKI
HHHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]