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Identifier: 15679550

GI number: 15679550

Start: 1421167

End: 1422177

Strand: Direct

Name: Not Available

Synonym: MTH1554

Alternate gene names: 15679550

Gene position: 1421167-1422177 (Clockwise)

Preceding gene: 15679549

Following gene: 15679551

Centisome position: 81.15

GC content: 48.86

Gene sequence:

>1011_bases
GTGGAGCGAACCGGTGAAGAAAACAGAAAGTTAATCTTCCAGGATTGCCTCTGCGCTGTTTGCGGACTCTGCGGTGAGAT
ATGTCCTGTGAACGCCATTGAAGTCAACCCGACAGGCGCAATGGTGAGAACCGAACAGGACGAGTCAAAGATATGCATCG
ATGAGAACAAATGCGTCCTCTGCGGTATGTGCAGCTCAATCTGCCCATTCCAGGCACTGGACCTTCAGATTGACGGAACA
TCAATAAAGGAACTTGCAGAATACCCAAAAATCATCAAATCAGCTGAGATAGATGATGAAACATGTATACAGTGCAAGGC
CTGTGAAACAGCATGTCCACAGGATGCCATAACCATAACAAGGGACCTGCCCGAAAGGAAGGACCTCATAACAGGTGAGA
TCGAGATAGACAAGGAAACCTGTATCTACTGTGGAATGTGTGAGGAGATGTGTCCGGTTGATGCGATAGAGATAGACCAC
CAGGTGCCGACCTCCTCAAGTCCAGCAGTCGCGACTGACATACGAGTCGATGAGGATAAATGTGTCCACTGCGGTATATG
TAAGAGGATATGCCCTGTTGACGCCATAACTCAGGTCTGCAGGATATGCCCATACGGTGAATACGAGATCAAGGTCCCGG
AGGTCACAGGAACCTCCTACATCGACCCTGAACTCTGTGTGAACTGTGGATGGTGCCAGGAGATATGCCCTGTTGACGCT
GCAACGGTGACAAAGCCCTTCGAGGGAGAACTCATAATCGACGAGGACACCTGCCAGGCCTGTGAAACCTGTGTGATGGC
ATGCCCATGCAACGTTTTATCATTCCCTAAACCAGAAAAACCAGGTGAAAAACCAGCCAAGCTCTACAAGGACGAAAGGT
TCTGCATATACTGCGGCGCATGTGAGAGGTCATGTCCCGTTGACGCAATAGAGGTTAAGAGGAGCAGGATAAATACCACT
CCAATCAAATCAAAGGCCTGGAAGAACGCCTTTGAATCATTACTCAAATAA

Upstream 100 bases:

>100_bases
TGTCTCATCTGTGTTGATATGTGTCCAACTGATTCTATAGAAATAAAGGAGGTAGAACTTGATGGAAACAACCGAAGTAA
TTGAAGGTAAGAACATTACC

Downstream 100 bases:

>100_bases
ATGGAGGTAATCAGATGGCAATAGGACTTAAGGCATACCCTGAACTCTGCCATGGATGTGGAAACTGCGTGATCGCATGT
CCAGTGAACGCCCTCAGAAG

Product: tungsten formylmethanofuran dehydrogenase, subunit F

Products: CO2; methanofuran; reduced acceptor

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MERTGEENRKLIFQDCLCAVCGLCGEICPVNAIEVNPTGAMVRTEQDESKICIDENKCVLCGMCSSICPFQALDLQIDGT
SIKELAEYPKIIKSAEIDDETCIQCKACETACPQDAITITRDLPERKDLITGEIEIDKETCIYCGMCEEMCPVDAIEIDH
QVPTSSSPAVATDIRVDEDKCVHCGICKRICPVDAITQVCRICPYGEYEIKVPEVTGTSYIDPELCVNCGWCQEICPVDA
ATVTKPFEGELIIDEDTCQACETCVMACPCNVLSFPKPEKPGEKPAKLYKDERFCIYCGACERSCPVDAIEVKRSRINTT
PIKSKAWKNAFESLLK

Sequences:

>Translated_336_residues
MERTGEENRKLIFQDCLCAVCGLCGEICPVNAIEVNPTGAMVRTEQDESKICIDENKCVLCGMCSSICPFQALDLQIDGT
SIKELAEYPKIIKSAEIDDETCIQCKACETACPQDAITITRDLPERKDLITGEIEIDKETCIYCGMCEEMCPVDAIEIDH
QVPTSSSPAVATDIRVDEDKCVHCGICKRICPVDAITQVCRICPYGEYEIKVPEVTGTSYIDPELCVNCGWCQEICPVDA
ATVTKPFEGELIIDEDTCQACETCVMACPCNVLSFPKPEKPGEKPAKLYKDERFCIYCGACERSCPVDAIEVKRSRINTT
PIKSKAWKNAFESLLK
>Mature_336_residues
MERTGEENRKLIFQDCLCAVCGLCGEICPVNAIEVNPTGAMVRTEQDESKICIDENKCVLCGMCSSICPFQALDLQIDGT
SIKELAEYPKIIKSAEIDDETCIQCKACETACPQDAITITRDLPERKDLITGEIEIDKETCIYCGMCEEMCPVDAIEIDH
QVPTSSSPAVATDIRVDEDKCVHCGICKRICPVDAITQVCRICPYGEYEIKVPEVTGTSYIDPELCVNCGWCQEICPVDA
ATVTKPFEGELIIDEDTCQACETCVMACPCNVLSFPKPEKPGEKPAKLYKDERFCIYCGACERSCPVDAIEVKRSRINTT
PIKSKAWKNAFESLLK

Specific function: Probable Electron Transfer Protein For Hydrogenase 3 [C]

COG id: COG1145

COG function: function code C; Ferredoxin

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 8 4Fe-4S ferredoxin-type domains [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR016459 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: 1.2.99.5

Molecular weight: Translated: 37130; Mature: 37130

Theoretical pI: Translated: 4.26; Mature: 4.26

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

11.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
12.8 %Cys+Met (Translated Protein)
11.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
12.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERTGEENRKLIFQDCLCAVCGLCGEICPVNAIEVNPTGAMVRTEQDESKICIDENKCVL
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEECCCCCCEEECCCCEEE
CGMCSSICPFQALDLQIDGTSIKELAEYPKIIKSAEIDDETCIQCKACETACPQDAITIT
EECHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCHHHHEEEEHHCCCCCCCCEEEE
RDLPERKDLITGEIEIDKETCIYCGMCEEMCPVDAIEIDHQVPTSSSPAVATDIRVDEDK
CCCCCCCCEEECEEEECCCCEEEECCHHHHCCCCEEECCCCCCCCCCCCEEEEEEECCCC
CVHCGICKRICPVDAITQVCRICPYGEYEIKVPEVTGTSYIDPELCVNCGWCQEICPVDA
CEEHHHHHHHCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHCCCCCHHHHCCCCH
ATVTKPFEGELIIDEDTCQACETCVMACPCNVLSFPKPEKPGEKPAKLYKDERFCIYCGA
HHCCCCCCCCEEEECHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCEEEEECC
CERSCPVDAIEVKRSRINTTPIKSKAWKNAFESLLK
CCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MERTGEENRKLIFQDCLCAVCGLCGEICPVNAIEVNPTGAMVRTEQDESKICIDENKCVL
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEECCCCCCEEECCCCEEE
CGMCSSICPFQALDLQIDGTSIKELAEYPKIIKSAEIDDETCIQCKACETACPQDAITIT
EECHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCHHHHEEEEHHCCCCCCCCEEEE
RDLPERKDLITGEIEIDKETCIYCGMCEEMCPVDAIEIDHQVPTSSSPAVATDIRVDEDK
CCCCCCCCEEECEEEECCCCEEEECCHHHHCCCCEEECCCCCCCCCCCCEEEEEEECCCC
CVHCGICKRICPVDAITQVCRICPYGEYEIKVPEVTGTSYIDPELCVNCGWCQEICPVDA
CEEHHHHHHHCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHCCCCCHHHHCCCCH
ATVTKPFEGELIIDEDTCQACETCVMACPCNVLSFPKPEKPGEKPAKLYKDERFCIYCGA
HHCCCCCCCCEEEECHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCEEEEECC
CERSCPVDAIEVKRSRINTTPIKSKAWKNAFESLLK
CCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formylmethanofuran; H2O; acceptor

Specific reaction: formylmethanofuran + H2O + acceptor = CO2 + methanofuran + reduced acceptor

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]