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Identifier: 15679478

GI number: 15679478

Start: 1337346

End: 1338863

Strand: Direct

Name: Not Available

Synonym: MTH1481

Alternate gene names: 15679478

Gene position: 1337346-1338863 (Clockwise)

Preceding gene: 15679477

Following gene: 15679479

Centisome position: 76.36

GC content: 51.19

Gene sequence:

>1518_bases
ATGTACATAGAAGAGGTTAAAGGAGAAATGAATCTCCCGGAGAATGTCAGGATATTTGACACAACACTGAGGGATGGTGA
ACAGACACCTGGAGTGGCCCTCACAGTGGAGGAAAAGACGGGTATAGCAAGGAAGCTCGACGCCCTTGGAGTCGATACAA
TCGAGGCAGGGTTCCCTGCAGCATCTCCAGGTGAAATGAACTCCATAAGGAGAATAGTTGAACTCGGACTTGAAGCAAAT
ATCTGTGGTTTGGCAAGGGTGCTCCGTGAGGACATAGACGCGGCAATAGACTGTGGAGTTGATTACATACACACCTTCAT
AGGGACATCACCACTCCACCGGGAATACAAGCTCAAAATGTCCCCTGATGAGATAATAGACAGGGCGGTCTTTGGAGTGG
AATATGCGGCTGAACATGGCCTGAAGGTTGAGTTCTCTGCGGAGGATGCAACAAGGACTGAATTTGATTACCTGGTTGAG
GTATACCGTGCAGCCGAGGATGCCGGTGCCGAGATCATAAACGTCCCGGATACCGTCGGGGTCATGATACCCCGGGCCAT
GAACCACCTGGTATCCCGGCTTAAGGAGGAACTCAGGGTACCCATAAGCGTGCACTGTCACAATGACTTCGGCCTGGCAG
TCGCCAACTCCCTTGCAGCGGTGGAGGCCGGGGCATCACAGGTACATGCAACAGTGAATGGTCTCGGTGAAAGGGCAGGA
AACGCTGCCCTTGAGGAGGTTGTAATGGCCCTCATAACCAGGTATGACCTCCCGGTGGATATAAGAACCGTGGAACTCGT
CAACATCTCAGAATTCGTCTCAAAGATAACCGGTGTCAGGATGCCCCCCAACAAGGCCATAGTCGGTGAAAACGCCTTTG
CACATGAAGCCGGCATACACGTGCATGGAGTCCTTGAAAAGGCTGAAACCTATGAACCAATAACCCCTGAGATGGTGGGC
CACAAGAGGAGGATAGTCCTTGGTAAACACACAGGAGCCAACGCCCTCAGATCAAAGCTCCAGGAATACGGAATAGAGAT
GAAGGAGGAACAGTTCTGCACACTCTATGAACAGGTCAAGAGGCTTGGAGATAAGGGTAAGAGGATCACGGATGCTGATC
TGAGGGCCATGGCCGTCACCATCCTTGGAAAGGCCAGCAGGGAAATAGTGAAGCTGGAGGGCATAGCAGTCATGACAGGG
GAGAGTGTGATGCCAACAGCCACAGTTAAGCTCAGAATCGGTGACGAGGTCAAGACAACATCCATGACAGGGGTTGGACC
TGTAGATGCTGCAATAAACGCCATACAAAGCCTTGTAAGTGAAACAGCAGACATAGAACTCGATGAATACAACATAGAGG
CCATAACTGGTGGTACAAACGCACTTGCAGAGGTCTTCGTTGTCATGAGTGACGGGGAGGGTAACAAGGCCACAGGAAGG
TCCACCAGGGAGGACATAGTCATGGCGAGTGTTGAGGCAGTACTGGACGCCATAAACAAGATTCTGAGCCTTAAATAG

Upstream 100 bases:

>100_bases
AACTTATAAGATATAACTTATAACATACTGTGTGTAAAAGCTTTTAATTGTAGCCCACAGAAATTTAAGCAGATAAAATG
CCAGACCACGGGGAATCAAG

Downstream 100 bases:

>100_bases
AAAAGTGATTGAGCTGGTATGATATGGGGCCAGTTTCAACCTGCAAGCTCTTCGGTGCCGTGAGGGCGGTGACAGGCATA
AAAAATGCAGTACCCCTCAT

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase 2; Alpha-isopropylmalate synthase 2

Number of amino acids: Translated: 505; Mature: 505

Protein sequence:

>505_residues
MYIEEVKGEMNLPENVRIFDTTLRDGEQTPGVALTVEEKTGIARKLDALGVDTIEAGFPAASPGEMNSIRRIVELGLEAN
ICGLARVLREDIDAAIDCGVDYIHTFIGTSPLHREYKLKMSPDEIIDRAVFGVEYAAEHGLKVEFSAEDATRTEFDYLVE
VYRAAEDAGAEIINVPDTVGVMIPRAMNHLVSRLKEELRVPISVHCHNDFGLAVANSLAAVEAGASQVHATVNGLGERAG
NAALEEVVMALITRYDLPVDIRTVELVNISEFVSKITGVRMPPNKAIVGENAFAHEAGIHVHGVLEKAETYEPITPEMVG
HKRRIVLGKHTGANALRSKLQEYGIEMKEEQFCTLYEQVKRLGDKGKRITDADLRAMAVTILGKASREIVKLEGIAVMTG
ESVMPTATVKLRIGDEVKTTSMTGVGPVDAAINAIQSLVSETADIELDEYNIEAITGGTNALAEVFVVMSDGEGNKATGR
STREDIVMASVEAVLDAINKILSLK

Sequences:

>Translated_505_residues
MYIEEVKGEMNLPENVRIFDTTLRDGEQTPGVALTVEEKTGIARKLDALGVDTIEAGFPAASPGEMNSIRRIVELGLEAN
ICGLARVLREDIDAAIDCGVDYIHTFIGTSPLHREYKLKMSPDEIIDRAVFGVEYAAEHGLKVEFSAEDATRTEFDYLVE
VYRAAEDAGAEIINVPDTVGVMIPRAMNHLVSRLKEELRVPISVHCHNDFGLAVANSLAAVEAGASQVHATVNGLGERAG
NAALEEVVMALITRYDLPVDIRTVELVNISEFVSKITGVRMPPNKAIVGENAFAHEAGIHVHGVLEKAETYEPITPEMVG
HKRRIVLGKHTGANALRSKLQEYGIEMKEEQFCTLYEQVKRLGDKGKRITDADLRAMAVTILGKASREIVKLEGIAVMTG
ESVMPTATVKLRIGDEVKTTSMTGVGPVDAAINAIQSLVSETADIELDEYNIEAITGGTNALAEVFVVMSDGEGNKATGR
STREDIVMASVEAVLDAINKILSLK
>Mature_505_residues
MYIEEVKGEMNLPENVRIFDTTLRDGEQTPGVALTVEEKTGIARKLDALGVDTIEAGFPAASPGEMNSIRRIVELGLEAN
ICGLARVLREDIDAAIDCGVDYIHTFIGTSPLHREYKLKMSPDEIIDRAVFGVEYAAEHGLKVEFSAEDATRTEFDYLVE
VYRAAEDAGAEIINVPDTVGVMIPRAMNHLVSRLKEELRVPISVHCHNDFGLAVANSLAAVEAGASQVHATVNGLGERAG
NAALEEVVMALITRYDLPVDIRTVELVNISEFVSKITGVRMPPNKAIVGENAFAHEAGIHVHGVLEKAETYEPITPEMVG
HKRRIVLGKHTGANALRSKLQEYGIEMKEEQFCTLYEQVKRLGDKGKRITDADLRAMAVTILGKASREIVKLEGIAVMTG
ESVMPTATVKLRIGDEVKTTSMTGVGPVDAAINAIQSLVSETADIELDEYNIEAITGGTNALAEVFVVMSDGEGNKATGR
STREDIVMASVEAVLDAINKILSLK

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786261, Length=506, Percent_Identity=41.304347826087, Blast_Score=347, Evalue=9e-97,
Organism=Saccharomyces cerevisiae, GI6320071, Length=356, Percent_Identity=33.4269662921348, Blast_Score=198, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6320019, Length=369, Percent_Identity=32.7913279132791, Blast_Score=197, Evalue=3e-51,
Organism=Saccharomyces cerevisiae, GI6324682, Length=559, Percent_Identity=24.865831842576, Blast_Score=125, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6324225, Length=556, Percent_Identity=23.9208633093525, Blast_Score=118, Evalue=2e-27,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU12_METTH (O27525)

Other databases:

- EMBL:   AE000666
- PIR:   D69064
- RefSeq:   NP_276595.1
- ProteinModelPortal:   O27525
- SMR:   O27525
- STRING:   O27525
- GeneID:   1471750
- GenomeReviews:   AE000666_GR
- KEGG:   mth:MTH1481
- NMPDR:   fig|187420.1.peg.1450
- eggNOG:   arNOG04336
- HOGENOM:   HBG701974
- OMA:   ENSFAHE
- PhylomeDB:   O27525
- ProtClustDB:   PRK00915
- BioCyc:   MTHE187420:MTH1481-MONOMER
- BRENDA:   2.3.3.13
- HAMAP:   MF_01025_A
- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR011830
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00917
- TIGRFAMs:   TIGR02090

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer; SSF110921 2-isopropylmalate_synth_dimer

EC number: =2.3.3.13

Molecular weight: Translated: 54675; Mature: 54675

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1; PS00816 AIPM_HOMOCIT_SYNTH_2; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYIEEVKGEMNLPENVRIFDTTLRDGEQTPGVALTVEEKTGIARKLDALGVDTIEAGFPA
CCHHHCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEECCCCHHHHHHHCCCCHHHCCCCC
ASPGEMNSIRRIVELGLEANICGLARVLREDIDAAIDCGVDYIHTFIGTSPLHREYKLKM
CCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCEEECC
SPDEIIDRAVFGVEYAAEHGLKVEFSAEDATRTEFDYLVEVYRAAEDAGAEIINVPDTVG
CHHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCHHH
VMIPRAMNHLVSRLKEELRVPISVHCHNDFGLAVANSLAAVEAGASQVHATVNGLGERAG
HHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCC
NAALEEVVMALITRYDLPVDIRTVELVNISEFVSKITGVRMPPNKAIVGENAFAHEAGIH
HHHHHHHHHHHHHHCCCCCCEEEEEEECHHHHHHHHHCCCCCCCCEEECCCCCHHHCCCE
VHGVLEKAETYEPITPEMVGHKRRIVLGKHTGANALRSKLQEYGIEMKEEQFCTLYEQVK
EEEHHHHHHCCCCCCHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHH
RLGDKGKRITDADLRAMAVTILGKASREIVKLEGIAVMTGESVMPTATVKLRIGDEVKTT
HHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEECCEEEEECCCCCCEEEEEEEECCCEEEC
SMTGVGPVDAAINAIQSLVSETADIELDEYNIEAITGGTNALAEVFVVMSDGEGNKATGR
CCCCCCHHHHHHHHHHHHHHHHCCCEEECEEEEEEECCHHHHEEEEEEEECCCCCCCCCC
STREDIVMASVEAVLDAINKILSLK
CCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MYIEEVKGEMNLPENVRIFDTTLRDGEQTPGVALTVEEKTGIARKLDALGVDTIEAGFPA
CCHHHCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEECCCCHHHHHHHCCCCHHHCCCCC
ASPGEMNSIRRIVELGLEANICGLARVLREDIDAAIDCGVDYIHTFIGTSPLHREYKLKM
CCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCEEECC
SPDEIIDRAVFGVEYAAEHGLKVEFSAEDATRTEFDYLVEVYRAAEDAGAEIINVPDTVG
CHHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCHHH
VMIPRAMNHLVSRLKEELRVPISVHCHNDFGLAVANSLAAVEAGASQVHATVNGLGERAG
HHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCC
NAALEEVVMALITRYDLPVDIRTVELVNISEFVSKITGVRMPPNKAIVGENAFAHEAGIH
HHHHHHHHHHHHHHCCCCCCEEEEEEECHHHHHHHHHCCCCCCCCEEECCCCCHHHCCCE
VHGVLEKAETYEPITPEMVGHKRRIVLGKHTGANALRSKLQEYGIEMKEEQFCTLYEQVK
EEEHHHHHHCCCCCCHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHH
RLGDKGKRITDADLRAMAVTILGKASREIVKLEGIAVMTGESVMPTATVKLRIGDEVKTT
HHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEECCEEEEECCCCCCEEEEEEEECCCEEEC
SMTGVGPVDAAINAIQSLVSETADIELDEYNIEAITGGTNALAEVFVVMSDGEGNKATGR
CCCCCCHHHHHHHHHHHHHHHHCCCEEECEEEEEEECCHHHHEEEEEEEECCCCCCCCCC
STREDIVMASVEAVLDAINKILSLK
CCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463