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The map label for this gene is ksgA

Identifier: 15679326

GI number: 15679326

Start: 1195304

End: 1196125

Strand: Direct

Name: ksgA

Synonym: MTH1326

Alternate gene names: 15679326

Gene position: 1195304-1196125 (Clockwise)

Preceding gene: 15679325

Following gene: 15679327

Centisome position: 68.25

GC content: 49.64

Gene sequence:

>822_bases
ATGTCAGGGCTCTACACCGAGACAAGGGAGGTGCTGAGGAAGTATGGTGTGAGGCTTAGAAGGAGCCTCGGCCAGAACTA
CCTCATAGATGAGGTCAAAAGGCAGCGCATACTTGAATACGCTGACCTCAGGGAAGATGACAGGGTCCTTGAGATAGGAC
CGGGTATAGGGACCCTCACACTTCCAATGGCAGAACTTGCTGGTCATGTCACCGCAATTGAGAGTGATCCCCTCATTGCA
GCTATCCTGATGGACAGGCTTCAGGTGGATAACGTTGATGTGATAGTCGGTGATGCCCTCAGGGTTGATTTTCCAGAATT
CAACAAGGTCGTATCCAACCTGCCCTACCAGATCTCCTCCCCCATAACCTTCAGGCTTCTGGAGCATGACTTTGAACTCG
CTGTTCTCATGTACCAGAAGGAATTTGCAAGGAGAATGGTTGCAGAGCCGGGTACAAGGGAGTACTCAAGACTGTCGGTC
ATGGTGCACTTCCTTGCAGAGGTTGAAATAGTTGACTACCTCAAACCGGGCTGTTTCTTCCCCCGTCCCAGGGTTGAATC
TGCGGTTGTAACCCTTAAACCTACGGGTTTCAGGGCCCCGGCATTCCTAGAGGATGTATGCCGTGCACTCTTCCAGCACA
GGAAGAAGAAAACCTCAAAATCCCTCAGAGAATCATTCCATGAAATAAGGACTGATCTGAGCTTTAATGAAGTTCTGAGG
GGTCTTCCACCAGAAATACTGGAGAAGAGGGTCTTTCAGCTGAGACCAGAGGATATACTTGAAATAGCAGAGCATATAGA
GGATCTCTCAGGTTCTTCCTGA

Upstream 100 bases:

>100_bases
TGAGGGCGCCAAACGGGGAAAACGTAAGTACAACATATTCACAAGACCGCCCCAGAAGAGGAGGGGCTGATCCTTCAATT
TAAATTTAGAATCTATTTTT

Downstream 100 bases:

>100_bases
GCTGGGCCTTCATCAGCACATCCCTGAGAAAACTATAAGTATTATGTAAACTATTTTTTACATAGGTGATTGTTTTGATA
ATAGCAAGACCCCAGTGGTT

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N',N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MSGLYTETREVLRKYGVRLRRSLGQNYLIDEVKRQRILEYADLREDDRVLEIGPGIGTLTLPMAELAGHVTAIESDPLIA
AILMDRLQVDNVDVIVGDALRVDFPEFNKVVSNLPYQISSPITFRLLEHDFELAVLMYQKEFARRMVAEPGTREYSRLSV
MVHFLAEVEIVDYLKPGCFFPRPRVESAVVTLKPTGFRAPAFLEDVCRALFQHRKKKTSKSLRESFHEIRTDLSFNEVLR
GLPPEILEKRVFQLRPEDILEIAEHIEDLSGSS

Sequences:

>Translated_273_residues
MSGLYTETREVLRKYGVRLRRSLGQNYLIDEVKRQRILEYADLREDDRVLEIGPGIGTLTLPMAELAGHVTAIESDPLIA
AILMDRLQVDNVDVIVGDALRVDFPEFNKVVSNLPYQISSPITFRLLEHDFELAVLMYQKEFARRMVAEPGTREYSRLSV
MVHFLAEVEIVDYLKPGCFFPRPRVESAVVTLKPTGFRAPAFLEDVCRALFQHRKKKTSKSLRESFHEIRTDLSFNEVLR
GLPPEILEKRVFQLRPEDILEIAEHIEDLSGSS
>Mature_272_residues
SGLYTETREVLRKYGVRLRRSLGQNYLIDEVKRQRILEYADLREDDRVLEIGPGIGTLTLPMAELAGHVTAIESDPLIAA
ILMDRLQVDNVDVIVGDALRVDFPEFNKVVSNLPYQISSPITFRLLEHDFELAVLMYQKEFARRMVAEPGTREYSRLSVM
VHFLAEVEIVDYLKPGCFFPRPRVESAVVTLKPTGFRAPAFLEDVCRALFQHRKKKTSKSLRESFHEIRTDLSFNEVLRG
LPPEILEKRVFQLRPEDILEIAEHIEDLSGSS

Specific function: Specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI7657198, Length=219, Percent_Identity=38.8127853881278, Blast_Score=146, Evalue=2e-35,
Organism=Homo sapiens, GI156415992, Length=252, Percent_Identity=26.5873015873016, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1786236, Length=243, Percent_Identity=32.5102880658436, Blast_Score=106, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI25146882, Length=209, Percent_Identity=43.0622009569378, Blast_Score=137, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI25141369, Length=257, Percent_Identity=30.3501945525292, Blast_Score=101, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6324989, Length=181, Percent_Identity=39.2265193370166, Blast_Score=127, Evalue=2e-30,
Organism=Drosophila melanogaster, GI21358017, Length=191, Percent_Identity=42.4083769633508, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI21357273, Length=242, Percent_Identity=28.099173553719, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_METTH (O27381)

Other databases:

- EMBL:   AE000666
- PIR:   C69043
- RefSeq:   NP_276443.1
- ProteinModelPortal:   O27381
- SMR:   O27381
- STRING:   O27381
- GeneID:   1471043
- GenomeReviews:   AE000666_GR
- KEGG:   mth:MTH1326
- NMPDR:   fig|187420.1.peg.1298
- eggNOG:   arNOG05894
- HOGENOM:   HBG319664
- OMA:   LAVLMYQ
- PhylomeDB:   O27381
- ProtClustDB:   PRK14896
- BioCyc:   MTHE187420:MTH1326-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: 2.1.1.- [C]

Molecular weight: Translated: 31345; Mature: 31213

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 26-26 BINDING 28-28 BINDING 53-53 BINDING 74-74 BINDING 98-98 BINDING 113-113

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGLYTETREVLRKYGVRLRRSLGQNYLIDEVKRQRILEYADLREDDRVLEIGPGIGTLT
CCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHH
LPMAELAGHVTAIESDPLIAAILMDRLQVDNVDVIVGDALRVDFPEFNKVVSNLPYQISS
HHHHHHCCCCEEECCCCHHHHHHHHHHCCCCCEEEECCHHCCCCHHHHHHHHCCCCCCCC
PITFRLLEHDFELAVLMYQKEFARRMVAEPGTREYSRLSVMVHFLAEVEIVDYLKPGCFF
CEEEHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PRPRVESAVVTLKPTGFRAPAFLEDVCRALFQHRKKKTSKSLRESFHEIRTDLSFNEVLR
CCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
GLPPEILEKRVFQLRPEDILEIAEHIEDLSGSS
CCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SGLYTETREVLRKYGVRLRRSLGQNYLIDEVKRQRILEYADLREDDRVLEIGPGIGTLT
CCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHH
LPMAELAGHVTAIESDPLIAAILMDRLQVDNVDVIVGDALRVDFPEFNKVVSNLPYQISS
HHHHHHCCCCEEECCCCHHHHHHHHHHCCCCCEEEECCHHCCCCHHHHHHHHCCCCCCCC
PITFRLLEHDFELAVLMYQKEFARRMVAEPGTREYSRLSVMVHFLAEVEIVDYLKPGCFF
CEEEHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PRPRVESAVVTLKPTGFRAPAFLEDVCRALFQHRKKKTSKSLRESFHEIRTDLSFNEVLR
CCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
GLPPEILEKRVFQLRPEDILEIAEHIEDLSGSS
CCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Dimethylation [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463