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Identifier: 15679265

GI number: 15679265

Start: 1145806

End: 1147005

Strand: Direct

Name: Not Available

Synonym: MTH1254

Alternate gene names: 15679265

Gene position: 1145806-1147005 (Clockwise)

Preceding gene: 15679245

Following gene: 15679266

Centisome position: 65.42

GC content: 52.92

Gene sequence:

>1200_bases
TTGAAGGTGATTGCTGTGGATAAAGTGGTTCTTGCTTTCAGTGGAGGCCTTGACACATCAGTATGTATCAAACTCCTCGA
GGAAAAGTATAACATGGAGGTAATAACGGCCTGTGTGGATGTTGGTCAGCCGCGGGATGAAATTGAAAGACCGGCCATGG
TTGCCAAGGAACTCGGAAACTACAGACACTACACAGTAGATGCAAGGCGGGAGTTCGCAGAGGACTACATATTTCCAGCC
ATAAAGGCCAATGCGGTTTACGAGGGTTACCCCCTCAGCACCGCCCTTGCAAGGCCCTTGATAGCTAAAAAGATAGTTGA
AGTGGCCGAGAAGGAGGGTGCATCTGCCATAGCCCATGGATGCACCGGTAAGGGTAACGACCAGTTCCGTTTCGAGGCCG
TTATAAGGTCAACGACGGACCTGGATGTCATCGCCCCCATAAGGGACCTCAACCTCACAAGGACAGAGGAGATGGAGTAC
GCCAGGTCCTGTGGGATACCGCTCCCCTCAGACAAGCTCTACAGTATCGATGAGAACCTCTGGGGACGTGCAATAGAGGG
GGACATCCTTGAAGACCCCATGGTTGAGCCCCCTGAGGATGCCTTTGAATGGACAAAGCCCATCCACGAGACCCCTGAGG
ATCCTGAGGTCATTGAAATCGGTTTCCACGGCGGGGTCCCGGTTGCATTGAATGGTGAGGAACTGGAGCCAGTTGAACTC
ATAGGCCTTGCAAATGAGGTGGCAGGAAAACACGGTATCGGTAGGGTTGACATAATGGAGGACCGTATAATAGGTATGAA
AAGCCGCGAAATCTATGAGACACCTGCAGCCTTCCTGCTCCTGGAGGCCCACAGGGGCCTGGAGCAGCTCACACTCACAA
GGAGTGAGCTCAGGTTTGCTGACATAATAAGCAGCACCTATGCTGAACTGGTGTACAGTGGGCTGTGGCATGACCCCCTC
CGTGAGGACCTTGACATGGCAGTAAACCACATGCAGAGGCGTGTCACCGGTACAGTCAGGGTTAAACTCCACAGGGGCAG
CATGCGCGTCATTGGAAGGGAGTCACCCTACAGCCTGTACAGTGAGGAGATAGTTTCATTTGAGGATAAAACCCTCGATC
AGAGGGAGATGGCCGGGATGGTCAAAAACTATGCCCTCCAGGCTGCGATATACAGCAGGGTGTGCAGGAAGGAGAACTGA

Upstream 100 bases:

>100_bases
ACCTGCGGCAGGTGGGACATACACTTATAACTTATCCCATATTAATATCTATAGTGCTGTAATAAAAACATGAGCGTATC
AGAATGATTCCATTTATAAG

Downstream 100 bases:

>100_bases
TGGAAGGTCTCTCATTCCTCATACTCATTGTAATCGCCACTTTCATAGTTGCGGGGGGCCTGGCATCCCTGCGTATACTC
ATGGGTTTTGGTAAGAAGAT

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MKVIAVDKVVLAFSGGLDTSVCIKLLEEKYNMEVITACVDVGQPRDEIERPAMVAKELGNYRHYTVDARREFAEDYIFPA
IKANAVYEGYPLSTALARPLIAKKIVEVAEKEGASAIAHGCTGKGNDQFRFEAVIRSTTDLDVIAPIRDLNLTRTEEMEY
ARSCGIPLPSDKLYSIDENLWGRAIEGDILEDPMVEPPEDAFEWTKPIHETPEDPEVIEIGFHGGVPVALNGEELEPVEL
IGLANEVAGKHGIGRVDIMEDRIIGMKSREIYETPAAFLLLEAHRGLEQLTLTRSELRFADIISSTYAELVYSGLWHDPL
REDLDMAVNHMQRRVTGTVRVKLHRGSMRVIGRESPYSLYSEEIVSFEDKTLDQREMAGMVKNYALQAAIYSRVCRKEN

Sequences:

>Translated_399_residues
MKVIAVDKVVLAFSGGLDTSVCIKLLEEKYNMEVITACVDVGQPRDEIERPAMVAKELGNYRHYTVDARREFAEDYIFPA
IKANAVYEGYPLSTALARPLIAKKIVEVAEKEGASAIAHGCTGKGNDQFRFEAVIRSTTDLDVIAPIRDLNLTRTEEMEY
ARSCGIPLPSDKLYSIDENLWGRAIEGDILEDPMVEPPEDAFEWTKPIHETPEDPEVIEIGFHGGVPVALNGEELEPVEL
IGLANEVAGKHGIGRVDIMEDRIIGMKSREIYETPAAFLLLEAHRGLEQLTLTRSELRFADIISSTYAELVYSGLWHDPL
REDLDMAVNHMQRRVTGTVRVKLHRGSMRVIGRESPYSLYSEEIVSFEDKTLDQREMAGMVKNYALQAAIYSRVCRKEN
>Mature_399_residues
MKVIAVDKVVLAFSGGLDTSVCIKLLEEKYNMEVITACVDVGQPRDEIERPAMVAKELGNYRHYTVDARREFAEDYIFPA
IKANAVYEGYPLSTALARPLIAKKIVEVAEKEGASAIAHGCTGKGNDQFRFEAVIRSTTDLDVIAPIRDLNLTRTEEMEY
ARSCGIPLPSDKLYSIDENLWGRAIEGDILEDPMVEPPEDAFEWTKPIHETPEDPEVIEIGFHGGVPVALNGEELEPVEL
IGLANEVAGKHGIGRVDIMEDRIIGMKSREIYETPAAFLLLEAHRGLEQLTLTRSELRFADIISSTYAELVYSGLWHDPL
REDLDMAVNHMQRRVTGTVRVKLHRGSMRVIGRESPYSLYSEEIVSFEDKTLDQREMAGMVKNYALQAAIYSRVCRKEN

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI53759107, Length=404, Percent_Identity=40.8415841584158, Blast_Score=273, Evalue=3e-73,
Organism=Homo sapiens, GI16950633, Length=404, Percent_Identity=40.8415841584158, Blast_Score=273, Evalue=3e-73,
Organism=Escherichia coli, GI1789563, Length=373, Percent_Identity=30.5630026809651, Blast_Score=117, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6324514, Length=368, Percent_Identity=37.7717391304348, Blast_Score=225, Evalue=1e-59,
Organism=Drosophila melanogaster, GI21358151, Length=384, Percent_Identity=38.0208333333333, Blast_Score=234, Evalue=8e-62,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): ASSY_METTH (O27322)

Other databases:

- EMBL:   AE000666
- PIR:   F69034
- RefSeq:   NP_276382.1
- ProteinModelPortal:   O27322
- SMR:   O27322
- STRING:   O27322
- GeneID:   1471662
- GenomeReviews:   AE000666_GR
- KEGG:   mth:MTH1254
- NMPDR:   fig|187420.1.peg.1237
- eggNOG:   arNOG04108
- HOGENOM:   HBG335267
- OMA:   ADLVTFE
- ProtClustDB:   PRK13820
- BioCyc:   MTHE187420:MTH1254-MONOMER
- BRENDA:   6.3.4.5
- GO:   GO:0005737
- HAMAP:   MF_00005
- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11587
- TIGRFAMs:   TIGR00032

Pfam domain/function: PF00764 Arginosuc_synth

EC number: =6.3.4.5

Molecular weight: Translated: 44761; Mature: 44761

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1; PS00565 ARGININOSUCCIN_SYN_2

Important sites: BINDING 90-90 BINDING 120-120 BINDING 122-122 BINDING 126-126 BINDING 126-126 BINDING 127-127 BINDING 130-130 BINDING 175-175 BINDING 260-260 BINDING 272-272

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVIAVDKVVLAFSGGLDTSVCIKLLEEKYNMEVITACVDVGQPRDEIERPAMVAKELGN
CEEEEEEHEEEEECCCCCHHHHHHHHHHHHCHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
YRHYTVDARREFAEDYIFPAIKANAVYEGYPLSTALARPLIAKKIVEVAEKEGASAIAHG
CCEEEEHHHHHHHHHHHCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHC
CTGKGNDQFRFEAVIRSTTDLDVIAPIRDLNLTRTEEMEYARSCGIPLPSDKLYSIDENL
CCCCCCCCEEEHEEHHCCCCCEEECCHHHCCCCHHHHHHHHHHCCCCCCCCCCEECCHHH
WGRAIEGDILEDPMVEPPEDAFEWTKPIHETPEDPEVIEIGFHGGVPVALNGEELEPVEL
CCCCCCCCCCCCCCCCCCHHHHHHHCHHCCCCCCCCEEEEECCCCEEEEECCCCCCHHHH
IGLANEVAGKHGIGRVDIMEDRIIGMKSREIYETPAAFLLLEAHRGLEQLTLTRSELRFA
HHHHHHHHCCCCCCCEEEECCHHCCCCCCHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHH
DIISSTYAELVYSGLWHDPLREDLDMAVNHMQRRVTGTVRVKLHRGSMRVIGRESPYSLY
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCEEEEEEEECCCEEEEECCCCHHHH
SEEIVSFEDKTLDQREMAGMVKNYALQAAIYSRVCRKEN
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKVIAVDKVVLAFSGGLDTSVCIKLLEEKYNMEVITACVDVGQPRDEIERPAMVAKELGN
CEEEEEEHEEEEECCCCCHHHHHHHHHHHHCHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
YRHYTVDARREFAEDYIFPAIKANAVYEGYPLSTALARPLIAKKIVEVAEKEGASAIAHG
CCEEEEHHHHHHHHHHHCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHC
CTGKGNDQFRFEAVIRSTTDLDVIAPIRDLNLTRTEEMEYARSCGIPLPSDKLYSIDENL
CCCCCCCCEEEHEEHHCCCCCEEECCHHHCCCCHHHHHHHHHHCCCCCCCCCCEECCHHH
WGRAIEGDILEDPMVEPPEDAFEWTKPIHETPEDPEVIEIGFHGGVPVALNGEELEPVEL
CCCCCCCCCCCCCCCCCCHHHHHHHCHHCCCCCCCCEEEEECCCCEEEEECCCCCCHHHH
IGLANEVAGKHGIGRVDIMEDRIIGMKSREIYETPAAFLLLEAHRGLEQLTLTRSELRFA
HHHHHHHHCCCCCCCEEEECCHHCCCCCCHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHH
DIISSTYAELVYSGLWHDPLREDLDMAVNHMQRRVTGTVRVKLHRGSMRVIGRESPYSLY
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCEEEEEEEECCCEEEEECCCCHHHH
SEEIVSFEDKTLDQREMAGMVKNYALQAAIYSRVCRKEN
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463