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Identifier: 15679063

GI number: 15679063

Start: 956402

End: 958213

Strand: Direct

Name: Not Available

Synonym: MTH1050

Alternate gene names: 15679063

Gene position: 956402-958213 (Clockwise)

Preceding gene: 15679062

Following gene: 15679064

Centisome position: 54.61

GC content: 51.16

Gene sequence:

>1812_bases
GTGAGTAAAACCAAAATTTACATTAACGGGAAGCTTATAGGAACCTGTGAAAACCCTGAAGAATTTGTTGAGGAGATGCG
GAAAAAGAGGAGATCCGGAGAAGTCTCCCCTGAAATGAACATAACCCATTACCCTGAAAACCATGAGATTTACATATTCA
CAGACCCTGGAAGGGCCAGGAGGCCCCTGATCATAGTTGAGGACGGAGAACCTCTCCTCACAGAAGAACACCTTGAAAAG
CTTGAAACAGGGGAAATGTCATGGGACGACCTCATAGCCGCTGGAATAATAGAGTACTTGGACGCAGAGGAGGAGGAAAA
CGCCTACATAGCCATGAGCCCTGATGAAATCAATGACGAACACACCCACCTCGAAATAGATCCCTCCACGATGCTCGGTA
TCTGCGCAGGTATCATCCCCTTTGCAAACCACAACTCATCACCGAGGAACACCATGGAGGCGGGGATGACCAAGCAGGCC
CTGGGTCTATACGCATCAAACTATGACTTGAGGACAGATACACGTGCACATCTCCTCCACCACCCCCAGGTCCCCATAGT
CAAGACAAGGATAATAGATGTGACAGGCTACGATGAAAGACCATCAGGTCAGAACTTCGTTGTTGCGGTAATGTCCTATG
AGGGCTACAACATGGAGGACGCCCTCATACTCAACAAGGCATCCCTTGAGAGGGGCCTTGCAAGATCATCATTCTTCAGA
TCCTATGAGGCCGCCGAGAGGAGATACCCCGGTGGTCAGGAGGACCGCTTCGAGATACCAGAGAAGGGTGTCAGGGGCTA
CAGGTCAGAGAGCGACTACAGGCACCTCGATGAGGACGGTATAATAAACCCTGAGGCAGAGGTCTCATCCGGTGACGTCC
TGATAGGTAAAACATCACCACCACGTTTCCTTGAAGAGATAGATGAATTCGGGACCGTGGCAGAGCGAAGAAGGGAAACA
TCAGTAACCGTGCGGCATGGTGAAAAGGGTATAGTGGACGCCGTTCTCCTTACAGAAACCGTCGAGGGCAGCAGACTCGC
CAAGATCAGGGTCAGAGAACAGAGGCAGCCCGAGCTAGGTGATAAATTCGCCTCAAGACACGGTCAGAAGGGGGTTGTCG
GCCTCATAGTATCCCAGGAGGACATGCCCTTCACCGAGGACGGAATAGTGCCCGACCTCATAGTCAACCCCCACGCCATA
CCATCAAGGATGTCTGTGGGACAGGTCCTCGAGATGCTCGCAGGTAAGGCTGCATGCATGGAGGGCCGCCGGGTTGATGG
AACACCCTTCACAGGAGAGGACGAGGATGACATAAAGGAGGCCCTCAGGGCCAACGGATTTGAAACTGCAGGTGTTGAAT
CACTCTACAACGGCATAACCGGTGAGAGGATAGAGGCAGAGATATTCATTGGAGTGGCCTACTACCAGAAACTGCACCAC
ATGACAACAGACAAGGTGTATGCAAGATCCAGGGGTCCGGTCCAGGTCCTCACAAGACAGCCGACAGAGGGGAGGGCCAG
GGAAGGAGGTCTCAGATTCGGTGAAATGGAAAGAGACTGCCTTATCGCACATGGAGCAGCCCTCGCCCTTAAGGAAAGGC
TCCTTGATGAATCAGACAAGTATGAGGCACTCGTATGTGCAGACTGTGGTATGATAGCAGTCTATGACAAGATAAGGGAC
AAGAAATACTGTCCTATATGTGAGGACTCAGAATCATTCCCTGTGGAAGTATCATACGCCTTCAAATTACTCCTCGACGA
ACTCAAGAGTCTCTGTATCTTCCCGAAACTCATACTGGAAGATAAGGCATGA

Upstream 100 bases:

>100_bases
GCCCTGCTGGCAAAGATATCCGAGGGCTCAGATGCCAGTGAGGTTGAGGAGGTCATTAAAATGGGGGTTGTGAACTAATT
TTCCACCGGGAGGCTGTTAA

Downstream 100 bases:

>100_bases
TAATGGATTTAAGGGAATAACAAAAAGGAGAGAATACCTTGAGAGGAATTTTAAAGAAAATTTCCCAGATAAACTTTGGC
CTCATGTCCCCCGAGGATAT

Product: DNA-directed RNA polymerase subunit B'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 603; Mature: 602

Protein sequence:

>603_residues
MSKTKIYINGKLIGTCENPEEFVEEMRKKRRSGEVSPEMNITHYPENHEIYIFTDPGRARRPLIIVEDGEPLLTEEHLEK
LETGEMSWDDLIAAGIIEYLDAEEEENAYIAMSPDEINDEHTHLEIDPSTMLGICAGIIPFANHNSSPRNTMEAGMTKQA
LGLYASNYDLRTDTRAHLLHHPQVPIVKTRIIDVTGYDERPSGQNFVVAVMSYEGYNMEDALILNKASLERGLARSSFFR
SYEAAERRYPGGQEDRFEIPEKGVRGYRSESDYRHLDEDGIINPEAEVSSGDVLIGKTSPPRFLEEIDEFGTVAERRRET
SVTVRHGEKGIVDAVLLTETVEGSRLAKIRVREQRQPELGDKFASRHGQKGVVGLIVSQEDMPFTEDGIVPDLIVNPHAI
PSRMSVGQVLEMLAGKAACMEGRRVDGTPFTGEDEDDIKEALRANGFETAGVESLYNGITGERIEAEIFIGVAYYQKLHH
MTTDKVYARSRGPVQVLTRQPTEGRAREGGLRFGEMERDCLIAHGAALALKERLLDESDKYEALVCADCGMIAVYDKIRD
KKYCPICEDSESFPVEVSYAFKLLLDELKSLCIFPKLILEDKA

Sequences:

>Translated_603_residues
MSKTKIYINGKLIGTCENPEEFVEEMRKKRRSGEVSPEMNITHYPENHEIYIFTDPGRARRPLIIVEDGEPLLTEEHLEK
LETGEMSWDDLIAAGIIEYLDAEEEENAYIAMSPDEINDEHTHLEIDPSTMLGICAGIIPFANHNSSPRNTMEAGMTKQA
LGLYASNYDLRTDTRAHLLHHPQVPIVKTRIIDVTGYDERPSGQNFVVAVMSYEGYNMEDALILNKASLERGLARSSFFR
SYEAAERRYPGGQEDRFEIPEKGVRGYRSESDYRHLDEDGIINPEAEVSSGDVLIGKTSPPRFLEEIDEFGTVAERRRET
SVTVRHGEKGIVDAVLLTETVEGSRLAKIRVREQRQPELGDKFASRHGQKGVVGLIVSQEDMPFTEDGIVPDLIVNPHAI
PSRMSVGQVLEMLAGKAACMEGRRVDGTPFTGEDEDDIKEALRANGFETAGVESLYNGITGERIEAEIFIGVAYYQKLHH
MTTDKVYARSRGPVQVLTRQPTEGRAREGGLRFGEMERDCLIAHGAALALKERLLDESDKYEALVCADCGMIAVYDKIRD
KKYCPICEDSESFPVEVSYAFKLLLDELKSLCIFPKLILEDKA
>Mature_602_residues
SKTKIYINGKLIGTCENPEEFVEEMRKKRRSGEVSPEMNITHYPENHEIYIFTDPGRARRPLIIVEDGEPLLTEEHLEKL
ETGEMSWDDLIAAGIIEYLDAEEEENAYIAMSPDEINDEHTHLEIDPSTMLGICAGIIPFANHNSSPRNTMEAGMTKQAL
GLYASNYDLRTDTRAHLLHHPQVPIVKTRIIDVTGYDERPSGQNFVVAVMSYEGYNMEDALILNKASLERGLARSSFFRS
YEAAERRYPGGQEDRFEIPEKGVRGYRSESDYRHLDEDGIINPEAEVSSGDVLIGKTSPPRFLEEIDEFGTVAERRRETS
VTVRHGEKGIVDAVLLTETVEGSRLAKIRVREQRQPELGDKFASRHGQKGVVGLIVSQEDMPFTEDGIVPDLIVNPHAIP
SRMSVGQVLEMLAGKAACMEGRRVDGTPFTGEDEDDIKEALRANGFETAGVESLYNGITGERIEAEIFIGVAYYQKLHHM
TTDKVYARSRGPVQVLTRQPTEGRAREGGLRFGEMERDCLIAHGAALALKERLLDESDKYEALVCADCGMIAVYDKIRDK
KYCPICEDSESFPVEVSYAFKLLLDELKSLCIFPKLILEDKA

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. The B (B''+B' and beta) subunits have been implicated in DNA promoter recognition and also in nucleotide binding [H]

COG id: COG0085

COG function: function code K; DNA-directed RNA polymerase, beta subunit/140 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase beta chain family [H]

Homologues:

Organism=Homo sapiens, GI4505941, Length=610, Percent_Identity=45.5737704918033, Blast_Score=550, Evalue=1e-156,
Organism=Homo sapiens, GI238908505, Length=600, Percent_Identity=42.6666666666667, Blast_Score=506, Evalue=1e-143,
Organism=Homo sapiens, GI238908503, Length=600, Percent_Identity=42.6666666666667, Blast_Score=505, Evalue=1e-143,
Organism=Homo sapiens, GI212286172, Length=596, Percent_Identity=34.8993288590604, Blast_Score=339, Evalue=5e-93,
Organism=Homo sapiens, GI33469941, Length=596, Percent_Identity=34.8993288590604, Blast_Score=338, Evalue=6e-93,
Organism=Escherichia coli, GI1790419, Length=303, Percent_Identity=32.013201320132, Blast_Score=144, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17552304, Length=613, Percent_Identity=43.5562805872757, Blast_Score=544, Evalue=1e-155,
Organism=Caenorhabditis elegans, GI25144348, Length=607, Percent_Identity=44.3163097199341, Blast_Score=527, Evalue=1e-150,
Organism=Caenorhabditis elegans, GI17506623, Length=605, Percent_Identity=34.0495867768595, Blast_Score=306, Evalue=2e-83,
Organism=Saccharomyces cerevisiae, GI6324725, Length=641, Percent_Identity=44.1497659906396, Blast_Score=557, Evalue=1e-159,
Organism=Saccharomyces cerevisiae, GI6324781, Length=601, Percent_Identity=45.0915141430948, Blast_Score=521, Evalue=1e-148,
Organism=Saccharomyces cerevisiae, GI6325267, Length=470, Percent_Identity=39.3617021276596, Blast_Score=318, Evalue=1e-87,
Organism=Drosophila melanogaster, GI17136444, Length=611, Percent_Identity=45.8265139116203, Blast_Score=552, Evalue=1e-157,
Organism=Drosophila melanogaster, GI17647877, Length=599, Percent_Identity=43.7395659432387, Blast_Score=523, Evalue=1e-148,
Organism=Drosophila melanogaster, GI17136446, Length=620, Percent_Identity=32.5806451612903, Blast_Score=319, Evalue=4e-87,

Paralogues:

None

Copy number: 4233 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015712
- InterPro:   IPR007120
- InterPro:   IPR019969
- InterPro:   IPR007121
- InterPro:   IPR007646
- InterPro:   IPR007647
- InterPro:   IPR007641
- InterPro:   IPR014724 [H]

Pfam domain/function: PF04566 RNA_pol_Rpb2_4; PF04567 RNA_pol_Rpb2_5; PF00562 RNA_pol_Rpb2_6; PF04560 RNA_pol_Rpb2_7 [H]

EC number: =2.7.7.6 [H]

Molecular weight: Translated: 67688; Mature: 67557

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS01166 RNA_POL_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKTKIYINGKLIGTCENPEEFVEEMRKKRRSGEVSPEMNITHYPENHEIYIFTDPGRAR
CCCCEEEECCEEEECCCCHHHHHHHHHHHHHCCCCCCCCCEEECCCCCEEEEEECCCCCC
RPLIIVEDGEPLLTEEHLEKLETGEMSWDDLIAAGIIEYLDAEEEENAYIAMSPDEINDE
CCEEEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCC
HTHLEIDPSTMLGICAGIIPFANHNSSPRNTMEAGMTKQALGLYASNYDLRTDTRAHLLH
CCEEEECHHHHHHHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHEECCCCCCCCCHHHHHC
HPQVPIVKTRIIDVTGYDERPSGQNFVVAVMSYEGYNMEDALILNKASLERGLARSSFFR
CCCCCEEEEEEEEEECCCCCCCCCCEEEEEEEECCCCCCHHEEEEHHHHHHHHHHHHHHH
SYEAAERRYPGGQEDRFEIPEKGVRGYRSESDYRHLDEDGIINPEAEVSSGDVLIGKTSP
HHHHHHHCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCC
PRFLEEIDEFGTVAERRRETSVTVRHGEKGIVDAVLLTETVEGSRLAKIRVREQRQPELG
HHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHEEEHHCCCCCEEEEEEECCCCCCCHH
DKFASRHGQKGVVGLIVSQEDMPFTEDGIVPDLIVNPHAIPSRMSVGQVLEMLAGKAACM
HHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHCCHHHHH
EGRRVDGTPFTGEDEDDIKEALRANGFETAGVESLYNGITGERIEAEIFIGVAYYQKLHH
CCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHCCCCCCEEEEEEEEEHHHHHHHHH
MTTDKVYARSRGPVQVLTRQPTEGRAREGGLRFGEMERDCLIAHGAALALKERLLDESDK
CCHHHHHHCCCCCEEEEECCCCCCCCHHCCCCCCCCCCCEEECCCHHHHHHHHHCCCCCC
YEALVCADCGMIAVYDKIRDKKYCPICEDSESFPVEVSYAFKLLLDELKSLCIFPKLILE
CCEEEEECCCEEEEHHHHCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHC
DKA
CCC
>Mature Secondary Structure 
SKTKIYINGKLIGTCENPEEFVEEMRKKRRSGEVSPEMNITHYPENHEIYIFTDPGRAR
CCCEEEECCEEEECCCCHHHHHHHHHHHHHCCCCCCCCCEEECCCCCEEEEEECCCCCC
RPLIIVEDGEPLLTEEHLEKLETGEMSWDDLIAAGIIEYLDAEEEENAYIAMSPDEINDE
CCEEEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCC
HTHLEIDPSTMLGICAGIIPFANHNSSPRNTMEAGMTKQALGLYASNYDLRTDTRAHLLH
CCEEEECHHHHHHHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHEECCCCCCCCCHHHHHC
HPQVPIVKTRIIDVTGYDERPSGQNFVVAVMSYEGYNMEDALILNKASLERGLARSSFFR
CCCCCEEEEEEEEEECCCCCCCCCCEEEEEEEECCCCCCHHEEEEHHHHHHHHHHHHHHH
SYEAAERRYPGGQEDRFEIPEKGVRGYRSESDYRHLDEDGIINPEAEVSSGDVLIGKTSP
HHHHHHHCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCC
PRFLEEIDEFGTVAERRRETSVTVRHGEKGIVDAVLLTETVEGSRLAKIRVREQRQPELG
HHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHEEEHHCCCCCEEEEEEECCCCCCCHH
DKFASRHGQKGVVGLIVSQEDMPFTEDGIVPDLIVNPHAIPSRMSVGQVLEMLAGKAACM
HHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHCCHHHHH
EGRRVDGTPFTGEDEDDIKEALRANGFETAGVESLYNGITGERIEAEIFIGVAYYQKLHH
CCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHCCCCCCEEEEEEEEEHHHHHHHHH
MTTDKVYARSRGPVQVLTRQPTEGRAREGGLRFGEMERDCLIAHGAALALKERLLDESDK
CCHHHHHHCCCCCEEEEECCCCCCCCHHCCCCCCCCCCCEEECCCHHHHHHHHHCCCCCC
YEALVCADCGMIAVYDKIRDKKYCPICEDSESFPVEVSYAFKLLLDELKSLCIFPKLILE
CCEEEEECCCEEEEHHHHCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHC
DKA
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2843811; 2834336 [H]