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Identifier: 15679062

GI number: 15679062

Start: 954820

End: 956379

Strand: Direct

Name: Not Available

Synonym: MTH1049

Alternate gene names: 15679062

Gene position: 954820-956379 (Clockwise)

Preceding gene: 15679061

Following gene: 15679063

Centisome position: 54.52

GC content: 51.67

Gene sequence:

>1560_bases
TTGTTTTGGAGGAAGTCCATGAAAAAAAGTGCATGGGGATTGGTAGACGCGTTTTTTGATAAATACGATCTGGTGGACCA
CCACATCCATTCATACAACGACTTCGTAAGTAACCGCATACAGGAAATAATAGATACAAGCGAACCCATTGAACTGGAAC
AGGGAAAATACCGGGTCGAAACAGGAAAGGTGAGCATAGAGAAGCCCTTCATCAAGGAGGCTGACGGCTCAAAGAGCAAG
ATATATCCCACAGAGGCCCGGCTAAGAAACCTCACCTACTCAGCCCACATGAGCCTGGAGATGAGACTCCTCAAGGAGGG
GGGACCTGAAACGGAATTCGAAAAGGTATACATCGGTGAGCTGCCGGTGATGCTGAAATCGGAGATATGCCACCTCCATG
GCCTCAGCAGGAAGGAACTGATGGAGAAGGGAGAGGACCCCGCGGACCCTGGAGGCTACTTCATTGTTAATGGATCAGAG
AGGTCCATCGTCACGATGGAGGAGATAGCTCCCAACAAGATAATCCTTGAAAGAATCGGTGAAGAGGACGAGAACAGAGC
AAGGGCAATAGTAACATCCATAAGGAGTGGATTCAGGGCCAGAATATCCCTGGAGTACCGTAAGCCAAGGAAGACCGGTG
TTTTCCTGAGAATATCCTTCCCCTACGTTCCAGGGGAAATCCCGCTCGTCATACTCCTCAGGGCCCTTGGACTTGCAACT
GACCAGGAAATCATCACCAGCATATCAGACGACTTCAACTACCAGATGATAGCTGCAGACGATATACAGGTCTCCCTCGA
CAGGCTCAACCTCAATAAAAAGGAGATGAAGGAACTCGATGAAGAGGACCGGAGAGAATACCTCATAAGGAGCGCCATAA
AGTACATCGGTAACCGCGTCGCCAAGGGGATGACCGAGGACTACCGTATAAAAAGGGCTGAAGACGTTATAGACCGTTAC
CTCCTGCCCCACATAGGAACAGAACCCGAAAAGAGGCTGGAAAAGGCAACATACCTTGCCGAGATGACCGAGATGCTCCT
TCAGGTCATATCAGGGGAGCGCAGGCCACACGACAAGGACCACTACACAAACAAGAGGCTCAGGGTTTCAGGCGACCTCA
TGGAGGACCTCCTCAGGGTGGCCTTCACAAGCCTGAGCAGGGATATGAGCTACCAGCTCGAGAGGAGCCTTGCCAGGGGT
AAGGAACCATCCGTAAAACAGGCTGTGAGATCCGACGTGCTGACAGAGAACCTCAAGCACGCCATTGCAACCGGTAACTG
GGTTGGTGGAAGGGCAGGTGTGAGCCAGCTGCTGGACCGGACAAGTTACATGGGTACACTCTCCCACATGAGGAGAGTCG
TTTCACCCCTCAGCAGAAGCCAGCCCCACTTCGAGGCAAGGGACCTTCACCCCACACAGTTCGGTAAGATCTGTCCAAAC
GAAACCCCTGAGGGTCCAAACTGTGGTCTTGTTAAGAACCTCGCCCTGCTGGCAAAGATATCCGAGGGCTCAGATGCCAG
TGAGGTTGAGGAGGTCATTAAAATGGGGGTTGTGAACTAA

Upstream 100 bases:

>100_bases
ATAATCCGTAAAAGTCCGACTGCAGAGGAATTCGTAACCTACAGGCTTGTTCAGGACTAGTTTTTAAGATCAACACTGCA
GGATCATTTCATCATCTAAT

Downstream 100 bases:

>100_bases
TTTTCCACCGGGAGGCTGTTAAGTGAGTAAAACCAAAATTTACATTAACGGGAAGCTTATAGGAACCTGTGAAAACCCTG
AAGAATTTGTTGAGGAGATG

Product: DNA-directed RNA polymerase subunit beta''

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 519; Mature: 519

Protein sequence:

>519_residues
MFWRKSMKKSAWGLVDAFFDKYDLVDHHIHSYNDFVSNRIQEIIDTSEPIELEQGKYRVETGKVSIEKPFIKEADGSKSK
IYPTEARLRNLTYSAHMSLEMRLLKEGGPETEFEKVYIGELPVMLKSEICHLHGLSRKELMEKGEDPADPGGYFIVNGSE
RSIVTMEEIAPNKIILERIGEEDENRARAIVTSIRSGFRARISLEYRKPRKTGVFLRISFPYVPGEIPLVILLRALGLAT
DQEIITSISDDFNYQMIAADDIQVSLDRLNLNKKEMKELDEEDRREYLIRSAIKYIGNRVAKGMTEDYRIKRAEDVIDRY
LLPHIGTEPEKRLEKATYLAEMTEMLLQVISGERRPHDKDHYTNKRLRVSGDLMEDLLRVAFTSLSRDMSYQLERSLARG
KEPSVKQAVRSDVLTENLKHAIATGNWVGGRAGVSQLLDRTSYMGTLSHMRRVVSPLSRSQPHFEARDLHPTQFGKICPN
ETPEGPNCGLVKNLALLAKISEGSDASEVEEVIKMGVVN

Sequences:

>Translated_519_residues
MFWRKSMKKSAWGLVDAFFDKYDLVDHHIHSYNDFVSNRIQEIIDTSEPIELEQGKYRVETGKVSIEKPFIKEADGSKSK
IYPTEARLRNLTYSAHMSLEMRLLKEGGPETEFEKVYIGELPVMLKSEICHLHGLSRKELMEKGEDPADPGGYFIVNGSE
RSIVTMEEIAPNKIILERIGEEDENRARAIVTSIRSGFRARISLEYRKPRKTGVFLRISFPYVPGEIPLVILLRALGLAT
DQEIITSISDDFNYQMIAADDIQVSLDRLNLNKKEMKELDEEDRREYLIRSAIKYIGNRVAKGMTEDYRIKRAEDVIDRY
LLPHIGTEPEKRLEKATYLAEMTEMLLQVISGERRPHDKDHYTNKRLRVSGDLMEDLLRVAFTSLSRDMSYQLERSLARG
KEPSVKQAVRSDVLTENLKHAIATGNWVGGRAGVSQLLDRTSYMGTLSHMRRVVSPLSRSQPHFEARDLHPTQFGKICPN
ETPEGPNCGLVKNLALLAKISEGSDASEVEEVIKMGVVN
>Mature_519_residues
MFWRKSMKKSAWGLVDAFFDKYDLVDHHIHSYNDFVSNRIQEIIDTSEPIELEQGKYRVETGKVSIEKPFIKEADGSKSK
IYPTEARLRNLTYSAHMSLEMRLLKEGGPETEFEKVYIGELPVMLKSEICHLHGLSRKELMEKGEDPADPGGYFIVNGSE
RSIVTMEEIAPNKIILERIGEEDENRARAIVTSIRSGFRARISLEYRKPRKTGVFLRISFPYVPGEIPLVILLRALGLAT
DQEIITSISDDFNYQMIAADDIQVSLDRLNLNKKEMKELDEEDRREYLIRSAIKYIGNRVAKGMTEDYRIKRAEDVIDRY
LLPHIGTEPEKRLEKATYLAEMTEMLLQVISGERRPHDKDHYTNKRLRVSGDLMEDLLRVAFTSLSRDMSYQLERSLARG
KEPSVKQAVRSDVLTENLKHAIATGNWVGGRAGVSQLLDRTSYMGTLSHMRRVVSPLSRSQPHFEARDLHPTQFGKICPN
ETPEGPNCGLVKNLALLAKISEGSDASEVEEVIKMGVVN

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. The B (B''+B' and beta) subunits have been implicated in DNA promoter recognition and also in nucleotide binding

COG id: COG0085

COG function: function code K; DNA-directed RNA polymerase, beta subunit/140 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase beta chain family

Homologues:

Organism=Homo sapiens, GI4505941, Length=542, Percent_Identity=34.6863468634686, Blast_Score=315, Evalue=6e-86,
Organism=Homo sapiens, GI238908503, Length=503, Percent_Identity=29.4234592445328, Blast_Score=224, Evalue=2e-58,
Organism=Homo sapiens, GI238908505, Length=445, Percent_Identity=30.3370786516854, Blast_Score=203, Evalue=3e-52,
Organism=Escherichia coli, GI1790419, Length=168, Percent_Identity=30.952380952381, Blast_Score=90, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17552304, Length=536, Percent_Identity=33.2089552238806, Blast_Score=301, Evalue=5e-82,
Organism=Caenorhabditis elegans, GI25144348, Length=524, Percent_Identity=29.9618320610687, Blast_Score=221, Evalue=1e-57,
Organism=Caenorhabditis elegans, GI17506623, Length=534, Percent_Identity=25.4681647940075, Blast_Score=126, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6324725, Length=542, Percent_Identity=32.8413284132841, Blast_Score=297, Evalue=2e-81,
Organism=Saccharomyces cerevisiae, GI6324781, Length=511, Percent_Identity=31.3111545988258, Blast_Score=218, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6325267, Length=510, Percent_Identity=26.6666666666667, Blast_Score=128, Evalue=3e-30,
Organism=Drosophila melanogaster, GI17136444, Length=543, Percent_Identity=34.4383057090239, Blast_Score=311, Evalue=7e-85,
Organism=Drosophila melanogaster, GI17647877, Length=517, Percent_Identity=29.9806576402321, Blast_Score=210, Evalue=2e-54,
Organism=Drosophila melanogaster, GI17136446, Length=493, Percent_Identity=22.920892494929, Blast_Score=101, Evalue=1e-21,

Paralogues:

None

Copy number: 4233 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RPOB2_METTH (O27123)

Other databases:

- EMBL:   AE000666
- PIR:   D69006
- RefSeq:   NP_276179.1
- ProteinModelPortal:   O27123
- STRING:   O27123
- GeneID:   1471457
- GenomeReviews:   AE000666_GR
- KEGG:   mth:MTH1049
- NMPDR:   fig|187420.1.peg.1034
- eggNOG:   arNOG04791
- HOGENOM:   HBG496841
- OMA:   LAGDLME
- ProtClustDB:   PRK09606
- BioCyc:   MTHE187420:MTH1049-MONOMER
- BRENDA:   2.7.7.6
- InterPro:   IPR015712
- InterPro:   IPR007644
- InterPro:   IPR007642
- InterPro:   IPR007645
- PANTHER:   PTHR20856

Pfam domain/function: PF04563 RNA_pol_Rpb2_1; PF04561 RNA_pol_Rpb2_2; PF04565 RNA_pol_Rpb2_3

EC number: =2.7.7.6

Molecular weight: Translated: 59192; Mature: 59192

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFWRKSMKKSAWGLVDAFFDKYDLVDHHIHSYNDFVSNRIQEIIDTSEPIELEQGKYRVE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCEEEE
TGKVSIEKPFIKEADGSKSKIYPTEARLRNLTYSAHMSLEMRLLKEGGPETEFEKVYIGE
ECCEEECCCCHHCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHEEEECC
LPVMLKSEICHLHGLSRKELMEKGEDPADPGGYFIVNGSERSIVTMEEIAPNKIILERIG
CCHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCEEEEECCCCCEEEHHHHCCCHHHHHHHC
EEDENRARAIVTSIRSGFRARISLEYRKPRKTGVFLRISFPYVPGEIPLVILLRALGLAT
CCCHHHHHHHHHHHHHCCEEEEEEEECCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCC
DQEIITSISDDFNYQMIAADDIQVSLDRLNLNKKEMKELDEEDRREYLIRSAIKYIGNRV
CHHHHHHHCCCCCEEEEEECCCEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKGMTEDYRIKRAEDVIDRYLLPHIGTEPEKRLEKATYLAEMTEMLLQVISGERRPHDKD
HHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH
HYTNKRLRVSGDLMEDLLRVAFTSLSRDMSYQLERSLARGKEPSVKQAVRSDVLTENLKH
HCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
AIATGNWVGGRAGVSQLLDRTSYMGTLSHMRRVVSPLSRSQPHFEARDLHPTQFGKICPN
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCC
ETPEGPNCGLVKNLALLAKISEGSDASEVEEVIKMGVVN
CCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MFWRKSMKKSAWGLVDAFFDKYDLVDHHIHSYNDFVSNRIQEIIDTSEPIELEQGKYRVE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCEEEE
TGKVSIEKPFIKEADGSKSKIYPTEARLRNLTYSAHMSLEMRLLKEGGPETEFEKVYIGE
ECCEEECCCCHHCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHEEEECC
LPVMLKSEICHLHGLSRKELMEKGEDPADPGGYFIVNGSERSIVTMEEIAPNKIILERIG
CCHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCEEEEECCCCCEEEHHHHCCCHHHHHHHC
EEDENRARAIVTSIRSGFRARISLEYRKPRKTGVFLRISFPYVPGEIPLVILLRALGLAT
CCCHHHHHHHHHHHHHCCEEEEEEEECCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCC
DQEIITSISDDFNYQMIAADDIQVSLDRLNLNKKEMKELDEEDRREYLIRSAIKYIGNRV
CHHHHHHHCCCCCEEEEEECCCEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKGMTEDYRIKRAEDVIDRYLLPHIGTEPEKRLEKATYLAEMTEMLLQVISGERRPHDKD
HHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH
HYTNKRLRVSGDLMEDLLRVAFTSLSRDMSYQLERSLARGKEPSVKQAVRSDVLTENLKH
HCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
AIATGNWVGGRAGVSQLLDRTSYMGTLSHMRRVVSPLSRSQPHFEARDLHPTQFGKICPN
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCC
ETPEGPNCGLVKNLALLAKISEGSDASEVEEVIKMGVVN
CCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463