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The map label for this gene is pgk

Identifier: 15679060

GI number: 15679060

Start: 952663

End: 953898

Strand: Direct

Name: pgk

Synonym: MTH1042

Alternate gene names: 15679060

Gene position: 952663-953898 (Clockwise)

Preceding gene: 15679059

Following gene: 15679061

Centisome position: 54.4

GC content: 52.83

Gene sequence:

>1236_bases
TTGATGGTGATCCCGGTGTCCTTTAAATTTAAAACCATGGATGACATTGAAGTCACAGGAAAGACAGTCCTTGTCCGCGT
GGACATAAATTCGCCTGTGGACCCCAATGATGGGACAATACTCGATGATACAAGGATGAGGCTCCATGCAGAGACCATAA
GGGAACTCTCAGATAGGGGTGCCAGGACAGTTGTGATGGCCCACCAGAGCAGACCCGGGAAGAATGACTTCACAACACTG
GAGCAGCATGCCAGGGCGCTCTCCGGGATACTCAGGAGGCCCGTTAAGTACGTTGAGGATATATTCGGATCCGCTGCAAG
GGAGAACATCTCAGGACTGGGTGATGGTGAGATAATCCTCCTTGAGAACGTCCGCTTCTACTCCGAGGAGGTGCTCAAGC
GAGACCCTGAGGAACAGGCGGAGACACACCTTGTTAGGAAGCTGGCGCCCCTCCTGGACTACTTCATCAACGACGCCTTC
GCGGCAGCCCACAGGTCCCAGCCCTCCCTTGTTGGCTTCGCCCTGAGGGTACCGTCAGCTGCGGGTCGTGTGATGGAGAG
GGAACTTCGAACACTTCAGGGTGCCCTTGAGAATGTTGAAAGGCCCTGTGTCTATGTTCTCGGCGGGGTGAAGGTTGATG
ACTCCATCATGGTCATGAAGAACGTCCTTGAAAACGGTAGCGCAGACCTTGTACTGACAACGGGCCTTGTTGCCAACATA
TTCCTGGCGGGATGCGGGGTTAAGATCGGTAAGGTGAACATGGACTTCATCAAGAGCAGGGGATACTGTGACTTCATCAA
GGTGGCGAAGAAACTGAAGAAGCGCTTCCCTGAAAGGATAGTTGTACCCGTTGATGTTGCAGTATGCAGGGACGGCAAGA
GGGTTGATGTTCCTGTGAAAAAGATACCCAACCATCCGATACAGGATATCGGAATGGAAACCATAAAGCTCTACGCCAGG
AGGATACGTGAGGCAAGGACCCTCTTTGCCAATGGACCGGCAGGCGTATTTGAGAACCCTGATTTCAGCATAGGAACCGA
GGACATACTGAATGCAATATCCTCTTCAGAAGGTTTCTCAATAATCGGCGGGGGTCACCTTGCTGCTGCAGCAGTGAAGA
TGGGCTTTGAGGACAGCATAAACCATATCAGCAGTGGAGGCGGTGCCAGCATAAGCCTCCTTGCAGGTGAGGAGCTTCCC
GCAGTCAGGGTGCTTGAGGAATCCAGGCACCCATAA

Upstream 100 bases:

>100_bases
AGGCTGCTGTTGACCTGGGGGCCGAGGGTGTTCTTCTTGCATCAGGCATCATCCTGGCTGACAGCCCACGGGACGCCCTT
CTGGATCTTGTAAGTAAGGT

Downstream 100 bases:

>100_bases
CCACAAACTATATAAATGAGTAGTTACTAACATTGGAAATGCCTCGGTAGCTCAGTCTGGTGGAGCGCGAGACTTGTAAT
CTCGTGGTCGCGGGTTCAAT

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 411; Mature: 411

Protein sequence:

>411_residues
MMVIPVSFKFKTMDDIEVTGKTVLVRVDINSPVDPNDGTILDDTRMRLHAETIRELSDRGARTVVMAHQSRPGKNDFTTL
EQHARALSGILRRPVKYVEDIFGSAARENISGLGDGEIILLENVRFYSEEVLKRDPEEQAETHLVRKLAPLLDYFINDAF
AAAHRSQPSLVGFALRVPSAAGRVMERELRTLQGALENVERPCVYVLGGVKVDDSIMVMKNVLENGSADLVLTTGLVANI
FLAGCGVKIGKVNMDFIKSRGYCDFIKVAKKLKKRFPERIVVPVDVAVCRDGKRVDVPVKKIPNHPIQDIGMETIKLYAR
RIREARTLFANGPAGVFENPDFSIGTEDILNAISSSEGFSIIGGGHLAAAAVKMGFEDSINHISSGGGASISLLAGEELP
AVRVLEESRHP

Sequences:

>Translated_411_residues
MMVIPVSFKFKTMDDIEVTGKTVLVRVDINSPVDPNDGTILDDTRMRLHAETIRELSDRGARTVVMAHQSRPGKNDFTTL
EQHARALSGILRRPVKYVEDIFGSAARENISGLGDGEIILLENVRFYSEEVLKRDPEEQAETHLVRKLAPLLDYFINDAF
AAAHRSQPSLVGFALRVPSAAGRVMERELRTLQGALENVERPCVYVLGGVKVDDSIMVMKNVLENGSADLVLTTGLVANI
FLAGCGVKIGKVNMDFIKSRGYCDFIKVAKKLKKRFPERIVVPVDVAVCRDGKRVDVPVKKIPNHPIQDIGMETIKLYAR
RIREARTLFANGPAGVFENPDFSIGTEDILNAISSSEGFSIIGGGHLAAAAVKMGFEDSINHISSGGGASISLLAGEELP
AVRVLEESRHP
>Mature_411_residues
MMVIPVSFKFKTMDDIEVTGKTVLVRVDINSPVDPNDGTILDDTRMRLHAETIRELSDRGARTVVMAHQSRPGKNDFTTL
EQHARALSGILRRPVKYVEDIFGSAARENISGLGDGEIILLENVRFYSEEVLKRDPEEQAETHLVRKLAPLLDYFINDAF
AAAHRSQPSLVGFALRVPSAAGRVMERELRTLQGALENVERPCVYVLGGVKVDDSIMVMKNVLENGSADLVLTTGLVANI
FLAGCGVKIGKVNMDFIKSRGYCDFIKVAKKLKKRFPERIVVPVDVAVCRDGKRVDVPVKKIPNHPIQDIGMETIKLYAR
RIREARTLFANGPAGVFENPDFSIGTEDILNAISSSEGFSIIGGGHLAAAAVKMGFEDSINHISSGGGASISLLAGEELP
AVRVLEESRHP

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI31543397, Length=416, Percent_Identity=33.8942307692308, Blast_Score=183, Evalue=3e-46,
Organism=Homo sapiens, GI4505763, Length=415, Percent_Identity=33.2530120481928, Blast_Score=182, Evalue=4e-46,
Organism=Escherichia coli, GI1789294, Length=404, Percent_Identity=35.8910891089109, Blast_Score=196, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI17508823, Length=413, Percent_Identity=33.4140435835351, Blast_Score=203, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6319857, Length=420, Percent_Identity=35, Blast_Score=189, Evalue=8e-49,
Organism=Drosophila melanogaster, GI24581203, Length=421, Percent_Identity=32.3040380047506, Blast_Score=197, Evalue=1e-50,
Organism=Drosophila melanogaster, GI17136394, Length=426, Percent_Identity=32.6291079812207, Blast_Score=184, Evalue=7e-47,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_METTH (O27121)

Other databases:

- EMBL:   AE000666
- PIR:   B69006
- RefSeq:   NP_276177.1
- ProteinModelPortal:   O27121
- SMR:   O27121
- STRING:   O27121
- GeneID:   1471450
- GenomeReviews:   AE000666_GR
- KEGG:   mth:MTH1042
- NMPDR:   fig|187420.1.peg.1032
- eggNOG:   arNOG04384
- HOGENOM:   HBG453500
- OMA:   PGKKDFT
- ProtClustDB:   PRK00073
- BioCyc:   MTHE187420:MTH1042-MONOMER
- BRENDA:   2.7.2.3
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 44998; Mature: 44998

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 45-45 BINDING 125-125 BINDING 165-165 BINDING 338-338

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMVIPVSFKFKTMDDIEVTGKTVLVRVDINSPVDPNDGTILDDTRMRLHAETIRELSDRG
CEEEEEEEEECCCCCCEECCCEEEEEEECCCCCCCCCCEEECCHHHHHHHHHHHHHHCCC
ARTVVMAHQSRPGKNDFTTLEQHARALSGILRRPVKYVEDIFGSAARENISGLGDGEIIL
CEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
LENVRFYSEEVLKRDPEEQAETHLVRKLAPLLDYFINDAFAAAHRSQPSLVGFALRVPSA
EECCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHH
AGRVMERELRTLQGALENVERPCVYVLGGVKVDDSIMVMKNVLENGSADLVLTTGLVANI
HHHHHHHHHHHHHHHHHHCCCCEEEEEECEEECCHHHHHHHHHCCCCCCEEEHHHHHHHH
FLAGCGVKIGKVNMDFIKSRGYCDFIKVAKKLKKRFPERIVVPVDVAVCRDGKRVDVPVK
HHHHCCCEEECCCHHHHHCCCCHHHHHHHHHHHHHCCCEEEEECCCEECCCCCEECCCHH
KIPNHPIQDIGMETIKLYARRIREARTLFANGPAGVFENPDFSIGTEDILNAISSSEGFS
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCE
IIGGGHLAAAAVKMGFEDSINHISSGGGASISLLAGEELPAVRVLEESRHP
EEECCHHHHHHHHHCCHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHCCCC
>Mature Secondary Structure
MMVIPVSFKFKTMDDIEVTGKTVLVRVDINSPVDPNDGTILDDTRMRLHAETIRELSDRG
CEEEEEEEEECCCCCCEECCCEEEEEEECCCCCCCCCCEEECCHHHHHHHHHHHHHHCCC
ARTVVMAHQSRPGKNDFTTLEQHARALSGILRRPVKYVEDIFGSAARENISGLGDGEIIL
CEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
LENVRFYSEEVLKRDPEEQAETHLVRKLAPLLDYFINDAFAAAHRSQPSLVGFALRVPSA
EECCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHH
AGRVMERELRTLQGALENVERPCVYVLGGVKVDDSIMVMKNVLENGSADLVLTTGLVANI
HHHHHHHHHHHHHHHHHHCCCCEEEEEECEEECCHHHHHHHHHCCCCCCEEEHHHHHHHH
FLAGCGVKIGKVNMDFIKSRGYCDFIKVAKKLKKRFPERIVVPVDVAVCRDGKRVDVPVK
HHHHCCCEEECCCHHHHHCCCCHHHHHHHHHHHHHCCCEEEEECCCEECCCCCEECCCHH
KIPNHPIQDIGMETIKLYARRIREARTLFANGPAGVFENPDFSIGTEDILNAISSSEGFS
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCE
IIGGGHLAAAAVKMGFEDSINHISSGGGASISLLAGEELPAVRVLEESRHP
EEECCHHHHHHHHHCCHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463