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Identifier: 15678504

GI number: 15678504

Start: 416307

End: 417968

Strand: Direct

Name: Not Available

Synonym: MTH476

Alternate gene names: 15678504

Gene position: 416307-417968 (Clockwise)

Preceding gene: 15678503

Following gene: 15678509

Centisome position: 23.77

GC content: 57.16

Gene sequence:

>1662_bases
ATGGCTGACTTCAGGTGCACTGTATGCAACTACATATTCCATGAGGAGGCTGATGGGGAATTCGATTCACTCCCTGCTGA
CTGGCGATGCCCTGTCTGCAATGCGCCACGCACAGCCTTTGTGAGGGTTCCATCCACTCTTGAGGAGGGCGGCAGGACGG
TTTCTGAGGTCTTCATCTCCCAGCTGGCAGCTTGGGGTCTTAAATACGTTTTCGGCATCCCGGGCACATCCACCCTGGGA
CTCGTTGATGCCCTCAGAAGGAACGATGAGATAAGGTACATACAGGTGCGTCATGAGGCTGCAGCGGCCTTCATGGCCTC
CAGCTACGGTAAACTTACTGGTCAGCCCGCGGTCTGCATGGCTGTGGCCGGCCCCCGGGCATCGAACCTTATAACAGGGC
TCCTTGACGCGGCCCTTGACCGGGCCCCGGTTCTTGCAGTCACAGGACATGTCGAAACCTACAGGATAGGTACAGGTGCA
AGCCAGGAGATAGATCAGCATAGCCTCTTTGAGTCTTTCTCGGTCTACAACATGACCCTTGTGAGCCCGGATGAGGCAGC
TGAGATTGCCAGGGAGGCTGTGAAGCACGCCATCCTTGAGCGGGGTGTTTCACATGTTGACGTCCCCCGGGATGTCCAGA
CCATGGAGTGCACAGCCCCGGTGAAGCCCCTCAGGGGCATGATGGCGGAGGCTGCAGTCACACCCCCCAGGAATCGTCTG
AGGGCTGCAGCTGATCTGATAAACAGAGCAGAGCGGCCCGTGATAATAGCAGGCTTCGGTGCCCTGGAAGCAGCTGACAG
TGTTGTTGAACTTGCGGAGAGGATAGGGGCAGCCATCGTATCCACCTTCAGGGGTAAGGGCATCGTTGATAATGACTACC
CCCTCTACCTGGGCTGTCATGGTAGCCTGGGCTCGGCTGCTGCGGCCGAGGCTGTTAGGAAAGCAGATCTTCTCCTGGTT
ATCGGGTCATCATTCTCTGACCTCACCCGGATCCCGCCTCGGAGGACCCTCCAGGTCGACATCGACCCCATGATGGTAGC
GAGGCGCCACCCGGTTGAGCAGGGCCTCACAGGAAGGTCCTCCCTCATAGTTCAGGAACTTATATCCATGGTGGAGGAGA
AGGAAAGGGGGCCCTACCTGGCAGAACTGGGGGAACTCAGGGATGAATGGCTGGGACTCCTTGAATCAGAGGCTGATCCT
TCACTGAGGCCCATCAGGCCCCAGTACATCATATCAGTCCTGAACAGGGAACTGGATGATGATGCCATCATAACCCTTGA
TGTTGGTGAGAACGGATGGTGGTTCGGCCGGAACTTCCAGATGAAGAGCACCAGAGGCTTCTCTTTTCAGGTTACCTGGG
GGTCCATGGGTTTCGGTCTTCCGGCGGCCATCGCAGCACAGCTTGAATTCCCGGATAGACAGGTCGTTTGCATCAACAAC
AGGAACCTTGCAATGATAATGCAGGAGCAGCGTGTTGAAGGATTCCCTGTATGGCAGACAGAGCTCCAGGACTGTGACTT
CGCAGGTTTTGCAGAGAACTGCGGGGGCCGGGGCCTGAGGGTGGATGATCCCGGTGAACTGGAGGACTCCGTGAGGGAGG
CCCTGGGAACTGATGGTCCGGTCCTCGTGGACATTGAAACAGATCCACGGCGCTTCATCTGA

Upstream 100 bases:

>100_bases
GGATCAGACCTCACAGAGGATATAAAGAAAGCCCCACATGGGGCTGAACTCCTTGAAAGGTTCCCGGTTGTTGGTGTACT
CGCTGAGGGGGGAAGTGCTG

Downstream 100 bases:

>100_bases
CCTTCACAGGAGCAGGAAGGTCTCATCTGATTACATGAACCGCAGAGGCCTTGAACTCTATCCAGACCCTGGACCCGGTG
GTGATCCCCATATCATTGAG

Product: pyruvate dehydrogenase / acetolactate synthase

Products: 2-acetolactate; CO2

Alternate protein names: NA

Number of amino acids: Translated: 553; Mature: 552

Protein sequence:

>553_residues
MADFRCTVCNYIFHEEADGEFDSLPADWRCPVCNAPRTAFVRVPSTLEEGGRTVSEVFISQLAAWGLKYVFGIPGTSTLG
LVDALRRNDEIRYIQVRHEAAAAFMASSYGKLTGQPAVCMAVAGPRASNLITGLLDAALDRAPVLAVTGHVETYRIGTGA
SQEIDQHSLFESFSVYNMTLVSPDEAAEIAREAVKHAILERGVSHVDVPRDVQTMECTAPVKPLRGMMAEAAVTPPRNRL
RAAADLINRAERPVIIAGFGALEAADSVVELAERIGAAIVSTFRGKGIVDNDYPLYLGCHGSLGSAAAAEAVRKADLLLV
IGSSFSDLTRIPPRRTLQVDIDPMMVARRHPVEQGLTGRSSLIVQELISMVEEKERGPYLAELGELRDEWLGLLESEADP
SLRPIRPQYIISVLNRELDDDAIITLDVGENGWWFGRNFQMKSTRGFSFQVTWGSMGFGLPAAIAAQLEFPDRQVVCINN
RNLAMIMQEQRVEGFPVWQTELQDCDFAGFAENCGGRGLRVDDPGELEDSVREALGTDGPVLVDIETDPRRFI

Sequences:

>Translated_553_residues
MADFRCTVCNYIFHEEADGEFDSLPADWRCPVCNAPRTAFVRVPSTLEEGGRTVSEVFISQLAAWGLKYVFGIPGTSTLG
LVDALRRNDEIRYIQVRHEAAAAFMASSYGKLTGQPAVCMAVAGPRASNLITGLLDAALDRAPVLAVTGHVETYRIGTGA
SQEIDQHSLFESFSVYNMTLVSPDEAAEIAREAVKHAILERGVSHVDVPRDVQTMECTAPVKPLRGMMAEAAVTPPRNRL
RAAADLINRAERPVIIAGFGALEAADSVVELAERIGAAIVSTFRGKGIVDNDYPLYLGCHGSLGSAAAAEAVRKADLLLV
IGSSFSDLTRIPPRRTLQVDIDPMMVARRHPVEQGLTGRSSLIVQELISMVEEKERGPYLAELGELRDEWLGLLESEADP
SLRPIRPQYIISVLNRELDDDAIITLDVGENGWWFGRNFQMKSTRGFSFQVTWGSMGFGLPAAIAAQLEFPDRQVVCINN
RNLAMIMQEQRVEGFPVWQTELQDCDFAGFAENCGGRGLRVDDPGELEDSVREALGTDGPVLVDIETDPRRFI
>Mature_552_residues
ADFRCTVCNYIFHEEADGEFDSLPADWRCPVCNAPRTAFVRVPSTLEEGGRTVSEVFISQLAAWGLKYVFGIPGTSTLGL
VDALRRNDEIRYIQVRHEAAAAFMASSYGKLTGQPAVCMAVAGPRASNLITGLLDAALDRAPVLAVTGHVETYRIGTGAS
QEIDQHSLFESFSVYNMTLVSPDEAAEIAREAVKHAILERGVSHVDVPRDVQTMECTAPVKPLRGMMAEAAVTPPRNRLR
AAADLINRAERPVIIAGFGALEAADSVVELAERIGAAIVSTFRGKGIVDNDYPLYLGCHGSLGSAAAAEAVRKADLLLVI
GSSFSDLTRIPPRRTLQVDIDPMMVARRHPVEQGLTGRSSLIVQELISMVEEKERGPYLAELGELRDEWLGLLESEADPS
LRPIRPQYIISVLNRELDDDAIITLDVGENGWWFGRNFQMKSTRGFSFQVTWGSMGFGLPAAIAAQLEFPDRQVVCINNR
NLAMIMQEQRVEGFPVWQTELQDCDFAGFAENCGGRGLRVDDPGELEDSVREALGTDGPVLVDIETDPRRFI

Specific function: Unknown

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=564, Percent_Identity=24.645390070922, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI21361361, Length=490, Percent_Identity=22.6530612244898, Blast_Score=93, Evalue=6e-19,
Organism=Escherichia coli, GI1787096, Length=524, Percent_Identity=32.0610687022901, Blast_Score=246, Evalue=3e-66,
Organism=Escherichia coli, GI1790104, Length=533, Percent_Identity=29.8311444652908, Blast_Score=223, Evalue=4e-59,
Organism=Escherichia coli, GI87081685, Length=546, Percent_Identity=27.2893772893773, Blast_Score=181, Evalue=9e-47,
Organism=Escherichia coli, GI1786717, Length=532, Percent_Identity=24.0601503759398, Blast_Score=128, Evalue=1e-30,
Organism=Escherichia coli, GI1788716, Length=437, Percent_Identity=22.4256292906178, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17531299, Length=551, Percent_Identity=23.956442831216, Blast_Score=96, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17531301, Length=549, Percent_Identity=23.6794171220401, Blast_Score=96, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6323755, Length=560, Percent_Identity=28.2142857142857, Blast_Score=205, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6320816, Length=287, Percent_Identity=28.5714285714286, Blast_Score=103, Evalue=9e-23,
Organism=Drosophila melanogaster, GI19922626, Length=451, Percent_Identity=25.9423503325942, Blast_Score=112, Evalue=5e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 2.2.1.6

Molecular weight: Translated: 60507; Mature: 60376

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS50903 RUBREDOXIN_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADFRCTVCNYIFHEEADGEFDSLPADWRCPVCNAPRTAFVRVPSTLEEGGRTVSEVFIS
CCCCEEHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHCCHHHHHHHH
QLAAWGLKYVFGIPGTSTLGLVDALRRNDEIRYIQVRHEAAAAFMASSYGKLTGQPAVCM
HHHHHHHHEEECCCCCCHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHCCCCCCCCCEEE
AVAGPRASNLITGLLDAALDRAPVLAVTGHVETYRIGTGASQEIDQHSLFESFSVYNMTL
EECCCCHHHHHHHHHHHHHCCCCEEEEECCEEEEEECCCCCHHHHHHHHHHHHCEEEEEE
VSPDEAAEIAREAVKHAILERGVSHVDVPRDVQTMECTAPVKPLRGMMAEAAVTPPRNRL
ECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHCCCCCHHHH
RAAADLINRAERPVIIAGFGALEAADSVVELAERIGAAIVSTFRGKGIVDNDYPLYLGCH
HHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECC
GSLGSAAAAEAVRKADLLLVIGSSFSDLTRIPPRRTLQVDIDPMMVARRHPVEQGLTGRS
CCCCHHHHHHHHHHCCEEEEECCCCHHHHCCCCCCEEEEECCHHHHHHCCCHHHCCCCHH
SLIVQELISMVEEKERGPYLAELGELRDEWLGLLESEADPSLRPIRPQYIISVLNRELDD
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCC
DAIITLDVGENGWWFGRNFQMKSTRGFSFQVTWGSMGFGLPAAIAAQLEFPDRQVVCINN
CEEEEEEECCCCEEECCCEEECCCCCCEEEEEECCCCCCCHHHHHEEECCCCCEEEEECC
RNLAMIMQEQRVEGFPVWQTELQDCDFAGFAENCGGRGLRVDDPGELEDSVREALGTDGP
CCEEEEEEHHCCCCCCCHHHHHCCCCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHCCCCC
VLVDIETDPRRFI
EEEEEECCCCCCC
>Mature Secondary Structure 
ADFRCTVCNYIFHEEADGEFDSLPADWRCPVCNAPRTAFVRVPSTLEEGGRTVSEVFIS
CCCEEHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHCCHHHHHHHH
QLAAWGLKYVFGIPGTSTLGLVDALRRNDEIRYIQVRHEAAAAFMASSYGKLTGQPAVCM
HHHHHHHHEEECCCCCCHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHCCCCCCCCCEEE
AVAGPRASNLITGLLDAALDRAPVLAVTGHVETYRIGTGASQEIDQHSLFESFSVYNMTL
EECCCCHHHHHHHHHHHHHCCCCEEEEECCEEEEEECCCCCHHHHHHHHHHHHCEEEEEE
VSPDEAAEIAREAVKHAILERGVSHVDVPRDVQTMECTAPVKPLRGMMAEAAVTPPRNRL
ECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHCCCCCHHHH
RAAADLINRAERPVIIAGFGALEAADSVVELAERIGAAIVSTFRGKGIVDNDYPLYLGCH
HHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECC
GSLGSAAAAEAVRKADLLLVIGSSFSDLTRIPPRRTLQVDIDPMMVARRHPVEQGLTGRS
CCCCHHHHHHHHHHCCEEEEECCCCHHHHCCCCCCEEEEECCHHHHHHCCCHHHCCCCHH
SLIVQELISMVEEKERGPYLAELGELRDEWLGLLESEADPSLRPIRPQYIISVLNRELDD
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCC
DAIITLDVGENGWWFGRNFQMKSTRGFSFQVTWGSMGFGLPAAIAAQLEFPDRQVVCINN
CEEEEEEECCCCEEECCCEEECCCCCCEEEEEECCCCCCCHHHHHEEECCCCCEEEEECC
RNLAMIMQEQRVEGFPVWQTELQDCDFAGFAENCGGRGLRVDDPGELEDSVREALGTDGP
CCEEEEEEHHCCCCCCCHHHHHCCCCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHCCCCC
VLVDIETDPRRFI
EEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pyruvate

Specific reaction: 2 pyruvate = 2-acetolactate + CO2

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]