Click here to switch to the map view.

The map label for this gene is Not Available

Identifier: 15678327

GI number: 15678327

Start: 233013

End: 235007

Strand: Direct

Name: Not Available

Synonym: MTH299

Alternate gene names: 15678327

Gene position: 233013-235007 (Clockwise)

Preceding gene: 15678326

Following gene: 15678328

Centisome position: 13.3

GC content: 50.38

Gene sequence:

>1995_bases
ATGAGGCCCTCAATCACCCTCAAAAACGGTGAAAGGTCAGAGGACGACCTGACCCATAAACTGGTCGATATACTCAGGAT
AAACCAGCGCCTCAAGGAGAACATGGAGGCAGGGGCTCCCCAGCTGATCGTGGAGGACCTCTGGGAACTCCTGCAGTACC
ATGTGACCACCTACTTCGATAACGAGGCCTCTGGTGTAACCCCTGCAAGGCAACGATCTGGAAGGCAGCTGCTGACGTTA
ATGCAGCGCTTAAAGAGCAAAAAGGGGATGTTCAGCAGTGAACTTTCAAAAGGACGGTTTAGGGGCAACCTTTCAGGTAA
GAGGGTTAACTTCTCTGCCCGTACTGTCATCTCCCCGGATCCCAACATCAGCATAAACGAGGTGGGTGTCCCTGAAATCA
TAGCCAGGGAGGTTACCGTACCGGTCTACGTCACAGAATGGAACATAGACCGTATGAGGGAGTACATAGAAAACGGGCCA
GACGTCCACCCGGGAGCCAACTACGTCATAAGACCCGACGGACGCAGGATAAGAATCTCCTCTAAAAACACGGAAACTGT
CATTGAGAACCTGAAACCTGGATACGTGGTTGAGAGACACCTCAAGGATGGTGACATCGTACTGTTCAACCGTCAACCAT
CCCTCCACAGGATGTCCCTCATGGCCCACCGGGCCCGTGTACTGCCCTACAAGACCTTCCGCCTCAACCTGTGTGTATGC
CCACCCTACAACGCCGACTTTGACGGTGACGAGATGAATATTCATGTACTGCAAACAGAGGAGTCCAGGGCAGAGGCAAA
AACCTTAATGCTCGTCCAAGAACACATGATATCCCCCAGATTCGGGGGGCCTATAATAGGTAGTATAAATGACCAGATAT
CTGGATTGTATCTCCTCACAAGAAAATTTGCAGAATTTGAAGAGGAAGAGGTATTCCAGCTACTTAGAAGCGCTGGAATT
TCATTACCATCTAGAAGGGGCACTAAATGGAGTGGTAAAGAAATATTTAGCCTGATACTCCCAGATGACCTCAGCATGAC
CTACAGGGCAGATGTATGCAGAAGATGTCAGGAATGCATCGAAGATGGATGTGAATATGATGCATATGTAGTAATAGAAA
ATGGAAGGCTCGAATCAGGCGTTATTGACAAAAAAACATGCGGGGCATTTTCAGGTAGATTGCTGGGACATATAATCAGG
GAATATGGTACTGATGAGGCCCGTGAATTCTTGGAATCAGCCAGCAGGCTGGCCATAGCAGTACTCATGAGGACAGGGTT
CACGACAAGTATCATGGACTTTGACGTGCCCGAAGAAGCTAGACAAATGATAGATTCAGTGCTTGGAATGGTTATGGATC
GTGTGGAAGGGCTCACTGAGGACAAGGAGGATGGGAGAGTCGAACCAGTGCAAGTAATGAGTTCGGAGAAGAACTTTGAG
ATTACGATAATGAAGGTCCTGGCCGGGGCAAGGGAGAGGGGAGGGGAAATAGCCAGGAAGTACCTTGGGGTGGATGGAAA
CCAGGGATATATGATAACCCGGACGGGTCTGAAGTGTTCATTGCTTAACTTCACCCAGATAACAGCCTGTTTGGGGCAGC
AGTCCGTCAGGGGTGAGCGTATCAGCAGGGGATACGATAACAGGACACTCCCTCACTTCAGGAAGGGAGAGCTCGGGGCA
AAGTCCCGGGGATTCGTACAATCAAGTTACAGGGAAGGTCTGGATCCCATTGAATTCTTCTTCCACGCCATGGGTGGAAG
GGATGCCCTCGTGGATACAGCCGTCCGCACAGCCCAGAGCGGTTACATGCAGAGGCGTCTCATAAACGCTCTCCAGGACC
TCACAGTCGATGAGGATGGCAGGGTAAGGGATAGCTGCGGTAACGTGATTCAGATACTCTACGGCGAGGACGGGGTAAGC
CCCTGGAGGGGGAAAAATGGCGAAATAGTGGATCTGGAACAGCTGGTCTATGAGATCAGGAGAAAAAGCCCATGA

Upstream 100 bases:

>100_bases
AGAGGATAAGCGATGACGACGCCCTCATCCTCGGTGTCAACCCCCAGGTTGCAAGGCCAGAATGGATGGTCCTGACAGTC
CTCCCGGTCCCCCCGTAACG

Downstream 100 bases:

>100_bases
ACTGATCTCTGATTCTGAAGCAGACCATTTAAGAGCCATCAGAGGAAATTCCTTAAAAAATACATACCAGCATCTAAAGA
CTTTATTTTTTTTGAATTGT

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 664; Mature: 664

Protein sequence:

>664_residues
MRPSITLKNGERSEDDLTHKLVDILRINQRLKENMEAGAPQLIVEDLWELLQYHVTTYFDNEASGVTPARQRSGRQLLTL
MQRLKSKKGMFSSELSKGRFRGNLSGKRVNFSARTVISPDPNISINEVGVPEIIAREVTVPVYVTEWNIDRMREYIENGP
DVHPGANYVIRPDGRRIRISSKNTETVIENLKPGYVVERHLKDGDIVLFNRQPSLHRMSLMAHRARVLPYKTFRLNLCVC
PPYNADFDGDEMNIHVLQTEESRAEAKTLMLVQEHMISPRFGGPIIGSINDQISGLYLLTRKFAEFEEEEVFQLLRSAGI
SLPSRRGTKWSGKEIFSLILPDDLSMTYRADVCRRCQECIEDGCEYDAYVVIENGRLESGVIDKKTCGAFSGRLLGHIIR
EYGTDEAREFLESASRLAIAVLMRTGFTTSIMDFDVPEEARQMIDSVLGMVMDRVEGLTEDKEDGRVEPVQVMSSEKNFE
ITIMKVLAGARERGGEIARKYLGVDGNQGYMITRTGLKCSLLNFTQITACLGQQSVRGERISRGYDNRTLPHFRKGELGA
KSRGFVQSSYREGLDPIEFFFHAMGGRDALVDTAVRTAQSGYMQRRLINALQDLTVDEDGRVRDSCGNVIQILYGEDGVS
PWRGKNGEIVDLEQLVYEIRRKSP

Sequences:

>Translated_664_residues
MRPSITLKNGERSEDDLTHKLVDILRINQRLKENMEAGAPQLIVEDLWELLQYHVTTYFDNEASGVTPARQRSGRQLLTL
MQRLKSKKGMFSSELSKGRFRGNLSGKRVNFSARTVISPDPNISINEVGVPEIIAREVTVPVYVTEWNIDRMREYIENGP
DVHPGANYVIRPDGRRIRISSKNTETVIENLKPGYVVERHLKDGDIVLFNRQPSLHRMSLMAHRARVLPYKTFRLNLCVC
PPYNADFDGDEMNIHVLQTEESRAEAKTLMLVQEHMISPRFGGPIIGSINDQISGLYLLTRKFAEFEEEEVFQLLRSAGI
SLPSRRGTKWSGKEIFSLILPDDLSMTYRADVCRRCQECIEDGCEYDAYVVIENGRLESGVIDKKTCGAFSGRLLGHIIR
EYGTDEAREFLESASRLAIAVLMRTGFTTSIMDFDVPEEARQMIDSVLGMVMDRVEGLTEDKEDGRVEPVQVMSSEKNFE
ITIMKVLAGARERGGEIARKYLGVDGNQGYMITRTGLKCSLLNFTQITACLGQQSVRGERISRGYDNRTLPHFRKGELGA
KSRGFVQSSYREGLDPIEFFFHAMGGRDALVDTAVRTAQSGYMQRRLINALQDLTVDEDGRVRDSCGNVIQILYGEDGVS
PWRGKNGEIVDLEQLVYEIRRKSP
>Mature_664_residues
MRPSITLKNGERSEDDLTHKLVDILRINQRLKENMEAGAPQLIVEDLWELLQYHVTTYFDNEASGVTPARQRSGRQLLTL
MQRLKSKKGMFSSELSKGRFRGNLSGKRVNFSARTVISPDPNISINEVGVPEIIAREVTVPVYVTEWNIDRMREYIENGP
DVHPGANYVIRPDGRRIRISSKNTETVIENLKPGYVVERHLKDGDIVLFNRQPSLHRMSLMAHRARVLPYKTFRLNLCVC
PPYNADFDGDEMNIHVLQTEESRAEAKTLMLVQEHMISPRFGGPIIGSINDQISGLYLLTRKFAEFEEEEVFQLLRSAGI
SLPSRRGTKWSGKEIFSLILPDDLSMTYRADVCRRCQECIEDGCEYDAYVVIENGRLESGVIDKKTCGAFSGRLLGHIIR
EYGTDEAREFLESASRLAIAVLMRTGFTTSIMDFDVPEEARQMIDSVLGMVMDRVEGLTEDKEDGRVEPVQVMSSEKNFE
ITIMKVLAGARERGGEIARKYLGVDGNQGYMITRTGLKCSLLNFTQITACLGQQSVRGERISRGYDNRTLPHFRKGELGA
KSRGFVQSSYREGLDPIEFFFHAMGGRDALVDTAVRTAQSGYMQRRLINALQDLTVDEDGRVRDSCGNVIQILYGEDGVS
PWRGKNGEIVDLEQLVYEIRRKSP

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates [H]

COG id: COG0086

COG function: function code K; DNA-directed RNA polymerase, beta' subunit/160 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase beta' chain family [H]

Homologues:

Organism=Homo sapiens, GI4505939, Length=674, Percent_Identity=41.5430267062315, Blast_Score=501, Evalue=1e-141,
Organism=Homo sapiens, GI39725938, Length=661, Percent_Identity=41.6036308623298, Blast_Score=491, Evalue=1e-139,
Organism=Homo sapiens, GI103471997, Length=683, Percent_Identity=32.6500732064422, Blast_Score=328, Evalue=1e-89,
Organism=Escherichia coli, GI2367335, Length=665, Percent_Identity=26.3157894736842, Blast_Score=175, Evalue=7e-45,
Organism=Caenorhabditis elegans, GI25145495, Length=684, Percent_Identity=39.766081871345, Blast_Score=489, Evalue=1e-138,
Organism=Caenorhabditis elegans, GI71987878, Length=656, Percent_Identity=39.9390243902439, Blast_Score=482, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI71998295, Length=612, Percent_Identity=31.8627450980392, Blast_Score=296, Evalue=3e-80,
Organism=Saccharomyces cerevisiae, GI6320061, Length=643, Percent_Identity=42.457231726283, Blast_Score=499, Evalue=1e-142,
Organism=Saccharomyces cerevisiae, GI6324690, Length=667, Percent_Identity=38.8305847076462, Blast_Score=457, Evalue=1e-129,
Organism=Saccharomyces cerevisiae, GI6324917, Length=601, Percent_Identity=32.7787021630616, Blast_Score=298, Evalue=2e-81,
Organism=Drosophila melanogaster, GI17530899, Length=669, Percent_Identity=40.0597907324365, Blast_Score=496, Evalue=1e-140,
Organism=Drosophila melanogaster, GI281360912, Length=684, Percent_Identity=40.2046783625731, Blast_Score=481, Evalue=1e-136,
Organism=Drosophila melanogaster, GI17647875, Length=614, Percent_Identity=34.8534201954397, Blast_Score=326, Evalue=3e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000722
- InterPro:   IPR006592
- InterPro:   IPR007080
- InterPro:   IPR007066
- InterPro:   IPR007083
- InterPro:   IPR007081
- InterPro:   IPR012758 [H]

Pfam domain/function: PF04997 RNA_pol_Rpb1_1; PF00623 RNA_pol_Rpb1_2; PF04983 RNA_pol_Rpb1_3; PF05000 RNA_pol_Rpb1_4; PF04998 RNA_pol_Rpb1_5 [H]

EC number: =2.7.7.6 [H]

Molecular weight: Translated: 75161; Mature: 75161

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPSITLKNGERSEDDLTHKLVDILRINQRLKENMEAGAPQLIVEDLWELLQYHVTTYFD
CCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEEC
NEASGVTPARQRSGRQLLTLMQRLKSKKGMFSSELSKGRFRGNLSGKRVNFSARTVISPD
CCCCCCCHHHHHCCHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCEEEEEEEEEECCC
PNISINEVGVPEIIAREVTVPVYVTEWNIDRMREYIENGPDVHPGANYVIRPDGRRIRIS
CCCEECCCCCHHHHHHHEEEEEEEEECCHHHHHHHHHCCCCCCCCCCEEECCCCCEEEEC
SKNTETVIENLKPGYVVERHLKDGDIVLFNRQPSLHRMSLMAHRARVLPYKTFRLNLCVC
CCCHHHHHHCCCCCCEEEEECCCCCEEEECCCCCHHHHHHHHHHHHHCEEEEEEEEEEEE
PPYNADFDGDEMNIHVLQTEESRAEAKTLMLVQEHMISPRFGGPIIGSINDQISGLYLLT
CCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCHHHHHHHHH
RKFAEFEEEEVFQLLRSAGISLPSRRGTKWSGKEIFSLILPDDLSMTYRADVCRRCQECI
HHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHEEECCCCCCHHHHHHHHHHHHHHH
EDGCEYDAYVVIENGRLESGVIDKKTCGAFSGRLLGHIIREYGTDEAREFLESASRLAIA
HCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
VLMRTGFTTSIMDFDVPEEARQMIDSVLGMVMDRVEGLTEDKEDGRVEPVQVMSSEKNFE
HHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCE
ITIMKVLAGARERGGEIARKYLGVDGNQGYMITRTGLKCSLLNFTQITACLGQQSVRGER
EEHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEECHHHHHHHHHCCHHHCHHH
ISRGYDNRTLPHFRKGELGAKSRGFVQSSYREGLDPIEFFFHAMGGRDALVDTAVRTAQS
HHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHH
GYMQRRLINALQDLTVDEDGRVRDSCGNVIQILYGEDGVSPWRGKNGEIVDLEQLVYEIR
HHHHHHHHHHHHHCCCCCCCCCHHCCCCEEEEEECCCCCCCCCCCCCCEEEHHHHHHHHH
RKSP
HCCC
>Mature Secondary Structure
MRPSITLKNGERSEDDLTHKLVDILRINQRLKENMEAGAPQLIVEDLWELLQYHVTTYFD
CCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEEC
NEASGVTPARQRSGRQLLTLMQRLKSKKGMFSSELSKGRFRGNLSGKRVNFSARTVISPD
CCCCCCCHHHHHCCHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCEEEEEEEEEECCC
PNISINEVGVPEIIAREVTVPVYVTEWNIDRMREYIENGPDVHPGANYVIRPDGRRIRIS
CCCEECCCCCHHHHHHHEEEEEEEEECCHHHHHHHHHCCCCCCCCCCEEECCCCCEEEEC
SKNTETVIENLKPGYVVERHLKDGDIVLFNRQPSLHRMSLMAHRARVLPYKTFRLNLCVC
CCCHHHHHHCCCCCCEEEEECCCCCEEEECCCCCHHHHHHHHHHHHHCEEEEEEEEEEEE
PPYNADFDGDEMNIHVLQTEESRAEAKTLMLVQEHMISPRFGGPIIGSINDQISGLYLLT
CCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCHHHHHHHHH
RKFAEFEEEEVFQLLRSAGISLPSRRGTKWSGKEIFSLILPDDLSMTYRADVCRRCQECI
HHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHEEECCCCCCHHHHHHHHHHHHHHH
EDGCEYDAYVVIENGRLESGVIDKKTCGAFSGRLLGHIIREYGTDEAREFLESASRLAIA
HCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
VLMRTGFTTSIMDFDVPEEARQMIDSVLGMVMDRVEGLTEDKEDGRVEPVQVMSSEKNFE
HHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCE
ITIMKVLAGARERGGEIARKYLGVDGNQGYMITRTGLKCSLLNFTQITACLGQQSVRGER
EEHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEECHHHHHHHHHCCHHHCHHH
ISRGYDNRTLPHFRKGELGAKSRGFVQSSYREGLDPIEFFFHAMGGRDALVDTAVRTAQS
HHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHH
GYMQRRLINALQDLTVDEDGRVRDSCGNVIQILYGEDGVSPWRGKNGEIVDLEQLVYEIR
HHHHHHHHHHHHHCCCCCCCCCHHCCCCEEEEEECCCCCCCCCCCCCCEEEHHHHHHHHH
RKSP
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]