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Identifier: 15678204

GI number: 15678204

Start: 122484

End: 124481

Strand: Direct

Name: Not Available

Synonym: MTH176

Alternate gene names: 15678204

Gene position: 122484-124481 (Clockwise)

Preceding gene: 15678203

Following gene: 15678205

Centisome position: 6.99

GC content: 53.9

Gene sequence:

>1998_bases
TTGATTTCACTGATCAGCAGGGGGAACACCCTGCTAATGACTTTATGGGAGGATTTCATGTTTGAAATAAAATCAAAGGA
CGGCCTCGGAAGGACGGGGATCCTTAAAACAGAGCATGGGACCGTAAGGACACCAGCGCTCATGCCTGTAATACATCCAG
GTAAACAGACCATCGATGTTAAGGGCCCCGGAGCCGAGATTGTGATAACGAATGCATACATAATCTACAGAAACCCTGAA
CTGAGGGAGAGGGCTCTCAGTGATGGAGTTCACAGACTGATAGACTTCGACGGACCCATAATGACGGATTCAGGTTCATT
CCAGCTATCAGAGTACGGGGACATTGAGGTCGAGAACCCTGAGATAATACGTTTCCAGGATGAGATAGGCACAGACATAG
GCACATCACTGGACATACCAACACCACCTGGTGTTAGCCACAGGAGGGCCATAGAGGAGGTTGAGGTCACCCTTGAGAGG
GCCAGGGAGTCCATTGAGTACAGGGAGAGAATGATGCTCAATGCCGTTGTACAGGGTTCAACACACCCTGACCTGAGGAG
GTACTGCGCATCACGCCTGGCCGAGCTACCGGTGGAGCTCCACCCCATCGGTGCTGTGGTGCCTCTAATGGAGTCCTACC
GCTACAGGGAACTCGTCGACGCCGTACTTTCATCGGTATCTGAGCTCCCACCCTCAAGGCCAAGGCACCTCATGGGGGCA
GGCCACCCAATGCTATTCGCCCTGGCAGTTTCAATGGGGTGCGACCTCTTTGATTCAGCGGCCTACATACTCTACGCAGA
GGATGACCGCCTCCTCAGCACTGAGGGAACATACAAACTTGAGAACCTCCAGGAGATGCCATGTTCCTGCAGTGTCTGCA
CGGATTACACACCATCAGAGCTCATGGGGATGGACAGGGAGGAGCGGAGGAACCTGATAGCGGAACACAACCTGCATGTG
AGCTTCGCAGAGATAAGGAAGGTCCGCCAGGCAATCCACGACGGAAACCTCATGGAACTGGTCGAAGAGCGCTGCAGGGC
ACACCCCCGCCTCCTTGAGGGGTACAGGCGCATGTCAGAGTACCTTGATCTCATTGAAAAATTTGAGCCCAGGTCCAAGA
GGTCGGCCTTCTTCTACACAGGCCCAGAGTCACTGGGGCGCGTGGAGGTCCACAGACACCTTAAAAGGGTTAAGGAGCAC
CTCGGGGAAAGATTAGCCCTCGTGGCCCCATCAAGGAGGCCCTACTCATCCAGCCTCCCCGCCAGGATCGGGGGTTTCAG
TTCACTGAGACCCCAGAGTGGGGGACCATGGAGGGTCGTGGTGGTGGACCTCCCCTTCGGTATAATCCCCCTGGAACTTG
ACCAGGTATACCCCCTGGCCCAGAGCGATGCCCCGGGTATAATGGACCTTGACGGGGAGGAGTTCCTTAGGGGCCTTGTC
AGGGACCTCATGGATGGAGATGCAATAGTGGACGATGCCCTCTGCAGCGAACTTGGAATTGAACTCCCCTACAAGTACAT
GGGTGAAGTTGAAACCACCGTTGATGACCTGGACCGTGTGAGGATGGTGGCAGACTACCAGTTCGGTATGGGCGCTGGTG
AACTCCTCTTCACCGATGATGTGAGGATTGAGAGGAGCCGCAACACGGGTAAGATAAGGCACATATATGCAGGGGATGAG
CTCATCTGCACCATGAGGGCATCAGATGGTCTCCTGGTCCTCGGGGCAGAGGGGGCGGTCAGGCTCCACAAGGGAACAGA
CTACCCTGCCTGGAGGGTGGCAGTAAATGAGGAGTCTGAACCCTTTGCAAGGAAGGGTAAAAGTGTTTTTGCAAAGTTTA
TAATAGACTGCGACAATAATATAAGGGCCAACGATGAAGTTTTAATTGTTAATGCTGATGATGAACTTCTGGCCACAGGC
AAGGCACTCCTCTGTGCCGAGGAGATGATGGACCTCAACCATGGCCAGGCAGTTAAAACCAGGAAAGGAGGATTTTAA

Upstream 100 bases:

>100_bases
ATTACATATTAATCAGTATCATCGTGGATATACACTGATCTGAGCATGTAGTATCTGGTGGTAGCCACTGCAGAGTACCT
GTTAATCAGTATCATTCTGC

Downstream 100 bases:

>100_bases
TTGATACCGGGTATGGGAATGAACCCCAAGCAGCTGAAACAGATGCAGAGGGCCATGAAGCAGATGGGAATGGATATGAA
GGATCTTCGTGGCGTTGAAG

Product: 7-cyano-7-deazaguanine tRNA-ribosyltransferase

Products: tRNAqueuine; guanine [C]

Alternate protein names: Archaeal tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 665; Mature: 665

Protein sequence:

>665_residues
MISLISRGNTLLMTLWEDFMFEIKSKDGLGRTGILKTEHGTVRTPALMPVIHPGKQTIDVKGPGAEIVITNAYIIYRNPE
LRERALSDGVHRLIDFDGPIMTDSGSFQLSEYGDIEVENPEIIRFQDEIGTDIGTSLDIPTPPGVSHRRAIEEVEVTLER
ARESIEYRERMMLNAVVQGSTHPDLRRYCASRLAELPVELHPIGAVVPLMESYRYRELVDAVLSSVSELPPSRPRHLMGA
GHPMLFALAVSMGCDLFDSAAYILYAEDDRLLSTEGTYKLENLQEMPCSCSVCTDYTPSELMGMDREERRNLIAEHNLHV
SFAEIRKVRQAIHDGNLMELVEERCRAHPRLLEGYRRMSEYLDLIEKFEPRSKRSAFFYTGPESLGRVEVHRHLKRVKEH
LGERLALVAPSRRPYSSSLPARIGGFSSLRPQSGGPWRVVVVDLPFGIIPLELDQVYPLAQSDAPGIMDLDGEEFLRGLV
RDLMDGDAIVDDALCSELGIELPYKYMGEVETTVDDLDRVRMVADYQFGMGAGELLFTDDVRIERSRNTGKIRHIYAGDE
LICTMRASDGLLVLGAEGAVRLHKGTDYPAWRVAVNEESEPFARKGKSVFAKFIIDCDNNIRANDEVLIVNADDELLATG
KALLCAEEMMDLNHGQAVKTRKGGF

Sequences:

>Translated_665_residues
MISLISRGNTLLMTLWEDFMFEIKSKDGLGRTGILKTEHGTVRTPALMPVIHPGKQTIDVKGPGAEIVITNAYIIYRNPE
LRERALSDGVHRLIDFDGPIMTDSGSFQLSEYGDIEVENPEIIRFQDEIGTDIGTSLDIPTPPGVSHRRAIEEVEVTLER
ARESIEYRERMMLNAVVQGSTHPDLRRYCASRLAELPVELHPIGAVVPLMESYRYRELVDAVLSSVSELPPSRPRHLMGA
GHPMLFALAVSMGCDLFDSAAYILYAEDDRLLSTEGTYKLENLQEMPCSCSVCTDYTPSELMGMDREERRNLIAEHNLHV
SFAEIRKVRQAIHDGNLMELVEERCRAHPRLLEGYRRMSEYLDLIEKFEPRSKRSAFFYTGPESLGRVEVHRHLKRVKEH
LGERLALVAPSRRPYSSSLPARIGGFSSLRPQSGGPWRVVVVDLPFGIIPLELDQVYPLAQSDAPGIMDLDGEEFLRGLV
RDLMDGDAIVDDALCSELGIELPYKYMGEVETTVDDLDRVRMVADYQFGMGAGELLFTDDVRIERSRNTGKIRHIYAGDE
LICTMRASDGLLVLGAEGAVRLHKGTDYPAWRVAVNEESEPFARKGKSVFAKFIIDCDNNIRANDEVLIVNADDELLATG
KALLCAEEMMDLNHGQAVKTRKGGF
>Mature_665_residues
MISLISRGNTLLMTLWEDFMFEIKSKDGLGRTGILKTEHGTVRTPALMPVIHPGKQTIDVKGPGAEIVITNAYIIYRNPE
LRERALSDGVHRLIDFDGPIMTDSGSFQLSEYGDIEVENPEIIRFQDEIGTDIGTSLDIPTPPGVSHRRAIEEVEVTLER
ARESIEYRERMMLNAVVQGSTHPDLRRYCASRLAELPVELHPIGAVVPLMESYRYRELVDAVLSSVSELPPSRPRHLMGA
GHPMLFALAVSMGCDLFDSAAYILYAEDDRLLSTEGTYKLENLQEMPCSCSVCTDYTPSELMGMDREERRNLIAEHNLHV
SFAEIRKVRQAIHDGNLMELVEERCRAHPRLLEGYRRMSEYLDLIEKFEPRSKRSAFFYTGPESLGRVEVHRHLKRVKEH
LGERLALVAPSRRPYSSSLPARIGGFSSLRPQSGGPWRVVVVDLPFGIIPLELDQVYPLAQSDAPGIMDLDGEEFLRGLV
RDLMDGDAIVDDALCSELGIELPYKYMGEVETTVDDLDRVRMVADYQFGMGAGELLFTDDVRIERSRNTGKIRHIYAGDE
LICTMRASDGLLVLGAEGAVRLHKGTDYPAWRVAVNEESEPFARKGKSVFAKFIIDCDNNIRANDEVLIVNADDELLATG
KALLCAEEMMDLNHGQAVKTRKGGF

Specific function: Exchanges the guanine residue with 7-cyano-7- deazaguanine (preQ0) at position 15 in the dihydrouridine loop (D- loop) of archaeal tRNAs [H]

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=353, Percent_Identity=24.6458923512748, Blast_Score=94, Evalue=4e-19,
Organism=Escherichia coli, GI1786607, Length=366, Percent_Identity=27.5956284153005, Blast_Score=124, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17544680, Length=364, Percent_Identity=23.3516483516484, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI19920510, Length=354, Percent_Identity=25.4237288135593, Blast_Score=97, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004804
- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01472 PUA; PF01702 TGT [H]

EC number: 2.4.2.29 [C]

Molecular weight: Translated: 74566; Mature: 74566

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISLISRGNTLLMTLWEDFMFEIKSKDGLGRTGILKTEHGTVRTPALMPVIHPGKQTIDV
CCEEECCCCEEEEHHHHHHHHHHHCCCCCCCCCEEECCCCCEECCCCCEEECCCCEEEEE
KGPGAEIVITNAYIIYRNPELRERALSDGVHRLIDFDGPIMTDSGSFQLSEYGDIEVENP
CCCCCEEEEEEEEEEEECCHHHHHHHHHHHHHHHCCCCCEEECCCCEEECCCCCEEECCC
EIIRFQDEIGTDIGTSLDIPTPPGVSHRRAIEEVEVTLERARESIEYRERMMLNAVVQGS
CEEEEEHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
THPDLRRYCASRLAELPVELHPIGAVVPLMESYRYRELVDAVLSSVSELPPSRPRHLMGA
CCHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCC
GHPMLFALAVSMGCDLFDSAAYILYAEDDRLLSTEGTYKLENLQEMPCSCSVCTDYTPSE
CCHHHHHHHHHHCCCCCCCCEEEEEECCCCEECCCCCEECCHHHHCCCCCEECCCCCCHH
LMGMDREERRNLIAEHNLHVSFAEIRKVRQAIHDGNLMELVEERCRAHPRLLEGYRRMSE
HHCCCHHHHHCHHHHCCCEEEHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHH
YLDLIEKFEPRSKRSAFFYTGPESLGRVEVHRHLKRVKEHLGERLALVAPSRRPYSSSLP
HHHHHHHCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCC
ARIGGFSSLRPQSGGPWRVVVVDLPFGIIPLELDQVYPLAQSDAPGIMDLDGEEFLRGLV
HHHCCCCCCCCCCCCCEEEEEEECCCCEEEEEHHCCCCCCCCCCCCCEECCHHHHHHHHH
RDLMDGDAIVDDALCSELGIELPYKYMGEVETTVDDLDRVRMVADYQFGMGAGELLFTDD
HHHCCCCHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHEEECCCCCCCCEEEECC
VRIERSRNTGKIRHIYAGDELICTMRASDGLLVLGAEGAVRLHKGTDYPAWRVAVNEESE
EEEEECCCCCCEEEEEECCEEEEEEECCCCEEEEECCCEEEEECCCCCCEEEEEECCCCC
PFARKGKSVFAKFIIDCDNNIRANDEVLIVNADDELLATGKALLCAEEMMDLNHGQAVKT
HHHHHHHHEEEHHEEECCCCCCCCCCEEEEECCCHHHHCCHHHHHHHHHHCCCCCCEECC
RKGGF
CCCCC
>Mature Secondary Structure
MISLISRGNTLLMTLWEDFMFEIKSKDGLGRTGILKTEHGTVRTPALMPVIHPGKQTIDV
CCEEECCCCEEEEHHHHHHHHHHHCCCCCCCCCEEECCCCCEECCCCCEEECCCCEEEEE
KGPGAEIVITNAYIIYRNPELRERALSDGVHRLIDFDGPIMTDSGSFQLSEYGDIEVENP
CCCCCEEEEEEEEEEEECCHHHHHHHHHHHHHHHCCCCCEEECCCCEEECCCCCEEECCC
EIIRFQDEIGTDIGTSLDIPTPPGVSHRRAIEEVEVTLERARESIEYRERMMLNAVVQGS
CEEEEEHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
THPDLRRYCASRLAELPVELHPIGAVVPLMESYRYRELVDAVLSSVSELPPSRPRHLMGA
CCHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCC
GHPMLFALAVSMGCDLFDSAAYILYAEDDRLLSTEGTYKLENLQEMPCSCSVCTDYTPSE
CCHHHHHHHHHHCCCCCCCCEEEEEECCCCEECCCCCEECCHHHHCCCCCEECCCCCCHH
LMGMDREERRNLIAEHNLHVSFAEIRKVRQAIHDGNLMELVEERCRAHPRLLEGYRRMSE
HHCCCHHHHHCHHHHCCCEEEHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHH
YLDLIEKFEPRSKRSAFFYTGPESLGRVEVHRHLKRVKEHLGERLALVAPSRRPYSSSLP
HHHHHHHCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCC
ARIGGFSSLRPQSGGPWRVVVVDLPFGIIPLELDQVYPLAQSDAPGIMDLDGEEFLRGLV
HHHCCCCCCCCCCCCCEEEEEEECCCCEEEEEHHCCCCCCCCCCCCCEECCHHHHHHHHH
RDLMDGDAIVDDALCSELGIELPYKYMGEVETTVDDLDRVRMVADYQFGMGAGELLFTDD
HHHCCCCHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHEEECCCCCCCCEEEECC
VRIERSRNTGKIRHIYAGDELICTMRASDGLLVLGAEGAVRLHKGTDYPAWRVAVNEESE
EEEEECCCCCCEEEEEECCEEEEEEECCCCEEEEECCCEEEEECCCCCCEEEEEECCCCC
PFARKGKSVFAKFIIDCDNNIRANDEVLIVNADDELLATGKALLCAEEMMDLNHGQAVKT
HHHHHHHHEEEHHEEECCCCCCCCCCEEEEECCCHHHHCCHHHHHHHHHHCCCCCCEECC
RKGGF
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Zn; Mg [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.00006 {guanine}} 0.000053 {guanine}} 0.000014 {7-(aminomethyl)-7-deazaguanine}} [C]

Substrates: tRNAguanine; queuine [C]

Specific reaction: tRNAguanine + queuine = tRNAqueuine + guanine [C]

General reaction: Pentosyl group transfer [C]

Inhibitor: Bisimidoester; Dimethyl suberimidate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]