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Identifier: 15678168

GI number: 15678168

Start: 91286

End: 92476

Strand: Direct

Name: Not Available

Synonym: MTH140

Alternate gene names: 15678168

Gene position: 91286-92476 (Clockwise)

Preceding gene: 15678167

Following gene: 15678169

Centisome position: 5.21

GC content: 54.07

Gene sequence:

>1191_bases
TTGAATGACAGTGAAATCCGCCAGTATGAGGATTTCTTCAGCGAAATCAATGAAAGACGCCTTGAACTTCTGGATAGGCC
AGAGGTGGCTGAACTGGCAGACAGGGTCAGGACGATACGTGAATCATCCATTGAGAACCTCAACGAACTCATAGCAGGGG
CATCTGAACGCTTCACGGAAAACGGCGTCGAGTTCCATCTTGCAGCTGACGCTGATGATGCCTGCAGGATAATATATGAG
AGCCTCGAAGGTGACACCATTGCAAAGTCAAAGTCCAACACCCTCTCCGAGATAGGGCTCGCAGATTACCTCAGGAGGAG
GGGGGTGGAGGTAATTGAGACGGACCTGGGGGACCGTATACTCCAGCTTGCAGGTATAGGAAAACCTGCGCACCCTGTTG
GACCGGCTCTCCACCTGGGTGTATCTGAAATAGCGGCCATAGTAAGGGATAAACTCGAAGCTGACATCAGGGATGATCCC
CATGAGATAATGGAGGCGGTGAGATCCGATATACTGCAGTCCATAGCTGAATGCAGTGCAGGGATAACCGGTGCCAATTC
TGTGGCAGCCTCTGATGGATCAGCTGTTATAGTCCATAACGAGGGAAACGTGGCCAGGCTCTCACTCATGGACACACACA
TCATGGTCTTTGGTGTGGATAAACTGGTCCCTCAGATCGAGGATGCTGTATCGGTGGTTAAACTGGAGACCGCCTATGCC
ACAGGTACCAGGGTGCCATCCTACATCAACGTGATCTCAGGACCCTCAAAGACCGCGGATATAGAGAAGATGCTCCTAAC
CGGGATGTACGGTGCATCAAGGGTCATAGCAGTTGCAGTTGACAACGGGAGGTCCGAAGCATACCCGGAGGCCCTCCTCT
GCATAGGGTGCGGCAGCTGCATAGTCAGCTGTCCCGTCTACAACACAGTCGGGAACAGGTTCGGTTACCGTGGATACCTG
GGGGGCCGTGGTGTTGTTATGAGCAGCTTCATTGATGGCAGGGAAGCTGCAGCTGAATCAGGCCTCTACATGTGCACCCT
GTGTGGTCTCTGCACCGAGAAATGCCCGGTTCGACCCCCCGCTGCTGAGATGATGGATAGGCTCCGGCGGAACTGCGTTG
CCGCGGGTATAAGGCACCCAAGCCACATGAGGATAGCGGCGCGGATACTCAGGAGGGGTTCACCCCTCTGA

Upstream 100 bases:

>100_bases
GCACCTCCTGTCCATTCTGCAGCCTCAACCTCGGAGCAAGATCCATGAGGGTCATGGACATTACAGATGCTCCTATCGGA
ACTTATCAGGAGGAGAAACG

Downstream 100 bases:

>100_bases
AAAGAAAGGTATAAAAAGGTGCTGTGCTAGATAAATTAAAAAAATTATCATATCATGTTTCTTGGGATATTCATTCCATG
ATTTTTACCAGGAGGTTATA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MNDSEIRQYEDFFSEINERRLELLDRPEVAELADRVRTIRESSIENLNELIAGASERFTENGVEFHLAADADDACRIIYE
SLEGDTIAKSKSNTLSEIGLADYLRRRGVEVIETDLGDRILQLAGIGKPAHPVGPALHLGVSEIAAIVRDKLEADIRDDP
HEIMEAVRSDILQSIAECSAGITGANSVAASDGSAVIVHNEGNVARLSLMDTHIMVFGVDKLVPQIEDAVSVVKLETAYA
TGTRVPSYINVISGPSKTADIEKMLLTGMYGASRVIAVAVDNGRSEAYPEALLCIGCGSCIVSCPVYNTVGNRFGYRGYL
GGRGVVMSSFIDGREAAAESGLYMCTLCGLCTEKCPVRPPAAEMMDRLRRNCVAAGIRHPSHMRIAARILRRGSPL

Sequences:

>Translated_396_residues
MNDSEIRQYEDFFSEINERRLELLDRPEVAELADRVRTIRESSIENLNELIAGASERFTENGVEFHLAADADDACRIIYE
SLEGDTIAKSKSNTLSEIGLADYLRRRGVEVIETDLGDRILQLAGIGKPAHPVGPALHLGVSEIAAIVRDKLEADIRDDP
HEIMEAVRSDILQSIAECSAGITGANSVAASDGSAVIVHNEGNVARLSLMDTHIMVFGVDKLVPQIEDAVSVVKLETAYA
TGTRVPSYINVISGPSKTADIEKMLLTGMYGASRVIAVAVDNGRSEAYPEALLCIGCGSCIVSCPVYNTVGNRFGYRGYL
GGRGVVMSSFIDGREAAAESGLYMCTLCGLCTEKCPVRPPAAEMMDRLRRNCVAAGIRHPSHMRIAARILRRGSPL
>Mature_396_residues
MNDSEIRQYEDFFSEINERRLELLDRPEVAELADRVRTIRESSIENLNELIAGASERFTENGVEFHLAADADDACRIIYE
SLEGDTIAKSKSNTLSEIGLADYLRRRGVEVIETDLGDRILQLAGIGKPAHPVGPALHLGVSEIAAIVRDKLEADIRDDP
HEIMEAVRSDILQSIAECSAGITGANSVAASDGSAVIVHNEGNVARLSLMDTHIMVFGVDKLVPQIEDAVSVVKLETAYA
TGTRVPSYINVISGPSKTADIEKMLLTGMYGASRVIAVAVDNGRSEAYPEALLCIGCGSCIVSCPVYNTVGNRFGYRGYL
GGRGVVMSSFIDGREAAAESGLYMCTLCGLCTEKCPVRPPAAEMMDRLRRNCVAAGIRHPSHMRIAARILRRGSPL

Specific function: Is essential for L-lactate degradation and allows cells to grow with lactate as the sole carbon source. Has probably a role as an electron transporter during oxidation of L-lactate. May also allow cells to utilize an alternative carbon source during biofi

COG id: COG1139

COG function: function code C; Uncharacterized conserved protein containing a ferredoxin-like domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1786498, Length=364, Percent_Identity=32.6923076923077, Blast_Score=177, Evalue=1e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017900
- InterPro:   IPR003741
- InterPro:   IPR004452
- InterPro:   IPR002698
- InterPro:   IPR012285
- InterPro:   IPR009051
- InterPro:   IPR022825 [H]

Pfam domain/function: PF02589 DUF162 [H]

EC number: NA

Molecular weight: Translated: 42841; Mature: 42841

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDSEIRQYEDFFSEINERRLELLDRPEVAELADRVRTIRESSIENLNELIAGASERFTE
CCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHC
NGVEFHLAADADDACRIIYESLEGDTIAKSKSNTLSEIGLADYLRRRGVEVIETDLGDRI
CCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHH
LQLAGIGKPAHPVGPALHLGVSEIAAIVRDKLEADIRDDPHEIMEAVRSDILQSIAECSA
HHHHCCCCCCCCCCCHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHC
GITGANSVAASDGSAVIVHNEGNVARLSLMDTHIMVFGVDKLVPQIEDAVSVVKLETAYA
CCCCCCCEECCCCCEEEEECCCCEEEEEEEHHEEEEEEHHHHHHHHHHHHHHHHHHHHHH
TGTRVPSYINVISGPSKTADIEKMLLTGMYGASRVIAVAVDNGRSEAYPEALLCIGCGSC
CCCCCCHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCHHHHEEECCHH
IVSCPVYNTVGNRFGYRGYLGGRGVVMSSFIDGREAAAESGLYMCTLCGLCTEKCPVRPP
HHCCCCHHHHHCCCCCCCCCCCCCHHHHHHHCHHHHHHHCCCHHHHHHHHHHCCCCCCCC
AAEMMDRLRRNCVAAGIRHPSHMRIAARILRRGSPL
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNDSEIRQYEDFFSEINERRLELLDRPEVAELADRVRTIRESSIENLNELIAGASERFTE
CCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHC
NGVEFHLAADADDACRIIYESLEGDTIAKSKSNTLSEIGLADYLRRRGVEVIETDLGDRI
CCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHH
LQLAGIGKPAHPVGPALHLGVSEIAAIVRDKLEADIRDDPHEIMEAVRSDILQSIAECSA
HHHHCCCCCCCCCCCHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHC
GITGANSVAASDGSAVIVHNEGNVARLSLMDTHIMVFGVDKLVPQIEDAVSVVKLETAYA
CCCCCCCEECCCCCEEEEECCCCEEEEEEEHHEEEEEEHHHHHHHHHHHHHHHHHHHHHH
TGTRVPSYINVISGPSKTADIEKMLLTGMYGASRVIAVAVDNGRSEAYPEALLCIGCGSC
CCCCCCHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCHHHHEEECCHH
IVSCPVYNTVGNRFGYRGYLGGRGVVMSSFIDGREAAAESGLYMCTLCGLCTEKCPVRPP
HHCCCCHHHHHCCCCCCCCCCCCCHHHHHHHCHHHHHHHCCCHHHHHHHHHHCCCCCCCC
AAEMMDRLRRNCVAAGIRHPSHMRIAARILRRGSPL
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]