Click here to switch to the map view.

The map label for this gene is Not Available

Identifier: 15678063

GI number: 15678063

Start: 16437

End: 17411

Strand: Direct

Name: Not Available

Synonym: MTH32

Alternate gene names: 15678063

Gene position: 16437-17411 (Clockwise)

Preceding gene: 15678062

Following gene: 15678064

Centisome position: 0.94

GC content: 55.79

Gene sequence:

>975_bases
TTGCAGGTATCCCTCATGGCAAACTTCATTGAACTAAAGGAGGCCACCACAAACCCTGACTACGGGTGCCCACCGGCTGA
ACGGGACATTGAGGCCCATATATCCATGGGTGTTGTGAACCTTGATAAACCCTCAGGTCCAACATCCCATCAGGTGGATT
CATGGGTCAGGGACATGCTCCACGTGGAGAAGGTGGGACACGGCGGCACACTCGACCCGAAGGTTACTGGTGTGCTCCCC
CTGGGAATAGACAGGGCCACCAGGGTCATGCAGCTCCTCCTGGAGGCTCCTAAGGAGTACGTTTGCCTCATGAGGGTCCA
CAGGGAGGTGGATGAGGAAAGAATAAGGGAGGTCCTGGGGGAATTTCAGGGGAAGATATTCCAGATACCCCCACTCAAAT
CCGCAGTTAAAAGGGATCTCCGCGTCAGAACAATCTACCGTGTGGATATACTGGAGGTGGATGGTCAGGATGTTCTCTTC
AGGATAGCCTGTGAGGCGGGGACCTATGTGAGAAAGTACTGCCACGATGTTGGCGAGGCCCTTGGTGCAGGTGCCCACAT
GGCTGAACTCAGACGCACAGCCGTGGGACCCTTCACAGAGGAGGGCCTTGTGACACTCCATGATCTCAAGGACGCATACC
AGTTCTGGGTTGAGGATGGAGATGAGACCTTCCTGAGGGAGTGCATCCTCCCCATGGAGTTTGCAGTGGGTCACCTCCCC
CGTGTGGTTATCCTGGACTCGGCGGTTGATGCAATATGCCATGGCGCTGATCTTGCACGTGGCGGTATAGCCGGACTGGA
TGATAACATAGCCTGGGGGGACACCGTCGCCATAATGACCCTCAAGGGCGAACTGGTGGGTGTTGGCGAGGCATCCATGT
CTGCACTGGATATTGCAGCGGCAGATGGTGGTCTTGTTATTGAAACCCGCAAGGTCTTCATGGAACCGGGAACCTACCCG
AGGATGTGGCGCTAG

Upstream 100 bases:

>100_bases
GCCACCTCGAACCCACTGAGAATAAGATAGAACTCAAGTCAGATGATATTGAGGAGATAAAGAAGGAACTTGAATCCCTG
GAATAAACTCACCTCTTTTT

Downstream 100 bases:

>100_bases
AAACCGACGGCAGCAATACTTAAATAGATGTCATAATATACAGTTATATGATACATCCTGTGCCGGGATAGCCTAGCCAG
GTAAGGCGCAAGACTGGAAA

Product: H/ACA RNA-protein complex component Cbf5p

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MQVSLMANFIELKEATTNPDYGCPPAERDIEAHISMGVVNLDKPSGPTSHQVDSWVRDMLHVEKVGHGGTLDPKVTGVLP
LGIDRATRVMQLLLEAPKEYVCLMRVHREVDEERIREVLGEFQGKIFQIPPLKSAVKRDLRVRTIYRVDILEVDGQDVLF
RIACEAGTYVRKYCHDVGEALGAGAHMAELRRTAVGPFTEEGLVTLHDLKDAYQFWVEDGDETFLRECILPMEFAVGHLP
RVVILDSAVDAICHGADLARGGIAGLDDNIAWGDTVAIMTLKGELVGVGEASMSALDIAAADGGLVIETRKVFMEPGTYP
RMWR

Sequences:

>Translated_324_residues
MQVSLMANFIELKEATTNPDYGCPPAERDIEAHISMGVVNLDKPSGPTSHQVDSWVRDMLHVEKVGHGGTLDPKVTGVLP
LGIDRATRVMQLLLEAPKEYVCLMRVHREVDEERIREVLGEFQGKIFQIPPLKSAVKRDLRVRTIYRVDILEVDGQDVLF
RIACEAGTYVRKYCHDVGEALGAGAHMAELRRTAVGPFTEEGLVTLHDLKDAYQFWVEDGDETFLRECILPMEFAVGHLP
RVVILDSAVDAICHGADLARGGIAGLDDNIAWGDTVAIMTLKGELVGVGEASMSALDIAAADGGLVIETRKVFMEPGTYP
RMWR
>Mature_324_residues
MQVSLMANFIELKEATTNPDYGCPPAERDIEAHISMGVVNLDKPSGPTSHQVDSWVRDMLHVEKVGHGGTLDPKVTGVLP
LGIDRATRVMQLLLEAPKEYVCLMRVHREVDEERIREVLGEFQGKIFQIPPLKSAVKRDLRVRTIYRVDILEVDGQDVLF
RIACEAGTYVRKYCHDVGEALGAGAHMAELRRTAVGPFTEEGLVTLHDLKDAYQFWVEDGDETFLRECILPMEFAVGHLP
RVVILDSAVDAICHGADLARGGIAGLDDNIAWGDTVAIMTLKGELVGVGEASMSALDIAAADGGLVIETRKVFMEPGTYP
RMWR

Specific function: Could be responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI4503337, Length=326, Percent_Identity=42.0245398773006, Blast_Score=243, Evalue=3e-64,
Organism=Homo sapiens, GI215599015, Length=326, Percent_Identity=42.0245398773006, Blast_Score=242, Evalue=3e-64,
Organism=Homo sapiens, GI21040257, Length=254, Percent_Identity=27.5590551181102, Blast_Score=95, Evalue=9e-20,
Organism=Escherichia coli, GI2367200, Length=254, Percent_Identity=30.7086614173228, Blast_Score=109, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17553978, Length=326, Percent_Identity=42.3312883435583, Blast_Score=263, Evalue=1e-70,
Organism=Saccharomyces cerevisiae, GI6323204, Length=306, Percent_Identity=45.7516339869281, Blast_Score=271, Evalue=9e-74,
Organism=Drosophila melanogaster, GI281364189, Length=326, Percent_Identity=41.4110429447853, Blast_Score=255, Evalue=3e-68,
Organism=Drosophila melanogaster, GI281364187, Length=326, Percent_Identity=41.4110429447853, Blast_Score=255, Evalue=3e-68,
Organism=Drosophila melanogaster, GI281364185, Length=326, Percent_Identity=41.4110429447853, Blast_Score=255, Evalue=3e-68,
Organism=Drosophila melanogaster, GI281364183, Length=326, Percent_Identity=41.4110429447853, Blast_Score=255, Evalue=3e-68,
Organism=Drosophila melanogaster, GI62471759, Length=326, Percent_Identity=41.4110429447853, Blast_Score=255, Evalue=3e-68,
Organism=Drosophila melanogaster, GI17975520, Length=326, Percent_Identity=41.4110429447853, Blast_Score=255, Evalue=3e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_METTH (O26140)

Other databases:

- EMBL:   AE000666
- PIR:   A69141
- RefSeq:   NP_275177.1
- ProteinModelPortal:   O26140
- SMR:   O26140
- STRING:   O26140
- GeneID:   1469994
- GenomeReviews:   AE000666_GR
- KEGG:   mth:MTH32
- NMPDR:   fig|187420.1.peg.32
- eggNOG:   arNOG04654
- HOGENOM:   HBG397258
- OMA:   SGPTSHE
- PhylomeDB:   O26140
- ProtClustDB:   PRK04270
- BioCyc:   MTHE187420:MTH32-MONOMER
- HAMAP:   MF_01081
- InterPro:   IPR012960
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR004802
- InterPro:   IPR002478
- InterPro:   IPR015947
- SMART:   SM00359
- TIGRFAMs:   TIGR00425

Pfam domain/function: PF08068 DKCLD; PF01472 PUA; PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom; SSF88697 PUA-like

EC number: 4.2.1.70

Molecular weight: Translated: 35733; Mature: 35733

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS50890 PUA

Important sites: ACT_SITE 72-72

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVSLMANFIELKEATTNPDYGCPPAERDIEAHISMGVVNLDKPSGPTSHQVDSWVRDML
CCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHEEEEEECCCCCCCCCHHHHHHHHHHH
HVEKVGHGGTLDPKVTGVLPLGIDRATRVMQLLLEAPKEYVCLMRVHREVDEERIREVLG
HHHHCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
EFQGKIFQIPPLKSAVKRDLRVRTIYRVDILEVDGQDVLFRIACEAGTYVRKYCHDVGEA
HHCCCEEECCCHHHHHHHCCEEEEEEEEEEEEECCCCEEEEEEHHCCHHHHHHHHHHHHH
LGAGAHMAELRRTAVGPFTEEGLVTLHDLKDAYQFWVEDGDETFLRECILPMEFAVGHLP
HCCCHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCC
RVVILDSAVDAICHGADLARGGIAGLDDNIAWGDTVAIMTLKGELVGVGEASMSALDIAA
EEEEEHHHHHHHHCCCHHHCCCCCCCCCCCCCCCEEEEEEECCCEEECCCHHHHHHEEEE
ADGGLVIETRKVFMEPGTYPRMWR
CCCCEEEEEHHEEECCCCCCCCCC
>Mature Secondary Structure
MQVSLMANFIELKEATTNPDYGCPPAERDIEAHISMGVVNLDKPSGPTSHQVDSWVRDML
CCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHEEEEEECCCCCCCCCHHHHHHHHHHH
HVEKVGHGGTLDPKVTGVLPLGIDRATRVMQLLLEAPKEYVCLMRVHREVDEERIREVLG
HHHHCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
EFQGKIFQIPPLKSAVKRDLRVRTIYRVDILEVDGQDVLFRIACEAGTYVRKYCHDVGEA
HHCCCEEECCCHHHHHHHCCEEEEEEEEEEEEECCCCEEEEEEHHCCHHHHHHHHHHHHH
LGAGAHMAELRRTAVGPFTEEGLVTLHDLKDAYQFWVEDGDETFLRECILPMEFAVGHLP
HCCCHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCC
RVVILDSAVDAICHGADLARGGIAGLDDNIAWGDTVAIMTLKGELVGVGEASMSALDIAA
EEEEEHHHHHHHHCCCHHHCCCCCCCCCCCCCCCEEEEEEECCCEEECCCHHHHHHEEEE
ADGGLVIETRKVFMEPGTYPRMWR
CCCCEEEEEHHEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463